Reviewed, UniProtKB/Swiss-Prot Q24106 (HID_DROME)

Names and origin

Protein names

Recommended name:
    Cell death protein W
Alternative name(s):
    Protein head involution defective
    Protein wrinkled

Gene names

Name:	W
Synonyms:	Hid
ORF Names:	CG5123

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Activator of apoptosis, with grim and rpr, that acts on the effector, Dredd. Seems to act genetically upstream of baculoviral anti-apoptotic p35. Ref.1 Ref.5
Developmental stage	Expression coincides with the onset of programmed cell death (PCD) at all stages of embryonic development, particularly in the head. Ref.1
Disruption phenotype	Mutants contain extra cells in the head owing to decreased levels of cell death and show a pronounced defect in the morphogenetic movements of head involution. Ectopic expression in the retina results in complete eye ablation. Ref.1
Sequence similarities	To D.melanogaster grim and rpr.

Ontologies

Keywords
Biological process	Apoptosis
Coding sequence diversity	Polymorphism
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	antennal morphogenesis Inferred from mutant phenotype. Source: FlyBase apoptosis Traceable author statement. Source: FlyBase compound eye retinal cell programmed cell death Traceable author statement. Source: FlyBase ecdysone-mediated induction of salivary gland cell autophagic cell death Inferred from expression pattern. Source: FlyBase entrainment of circadian clock by photoperiod Inferred from mutant phenotype. Source: FlyBase head involution Inferred from mutant phenotype. Source: FlyBase induction of apoptosis Inferred from mutant phenotype. Source: FlyBase larval midgut cell programmed cell death Inferred from genetic interaction. Source: FlyBase open tracheal system development Inferred from mutant phenotype. Source: FlyBase protein ubiquitination Traceable author statement. Source: FlyBase regulation of organ growth Inferred from mutant phenotype. Source: FlyBase regulation of proteolysis Traceable author statement. Source: FlyBase regulation of retinal cell programmed cell death Non-traceable author statement. Source: FlyBase response to red light Inferred from mutant phenotype. Source: FlyBase
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Ahcy13	Q27580	1	EBI-135509,EBI-171029
CG8414	Q9V7I8	1	EBI-135509,EBI-147266
th	Q24306	4	EBI-135509,EBI-456419

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 410	410	Cell death protein W		PRO_0000083972
Regions
	Compositional bias	17 – 79	63	Ser-rich
	Compositional bias	237 – 240	4	Poly-Ser
	Compositional bias	332 – 340	9	Poly-Ser
Amino acid modifications
	Modified residue	295	1	Phosphoserine Ref.6
Natural variations
	Natural variant	171	1	P → S in allele A22.
	Natural variant	261	1	S → L in allele A206.
Experimental info
	Sequence conflict	351	1	P → S in AAA79985. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q24106-1 [UniParc]. Last modified June 1, 2001. Version 2. Checksum: 63EBF913149E27E1	FASTA	410	43,877
	10 20 30 40 50 60 MAVPFYLPEG GADDVASSSS GASGNSSPHN HPLPSSASSS VSSSGVSSAS ASSASSSSSA 70 80 90 100 110 120 SSDGASSAAS QSPNTTTSSA TQTPMQSPLP TDQVLYALYE WVRMYQSQQS APQIFQYPPP 130 140 150 160 170 180 SPSCNFTGGD VFFPHGHPNP NSNPHPRTPR TSVSFSSGEE YNFFRQQQPQ PHPSYPAPST 190 200 210 220 230 240 PQPMPPQSAP PMHCSHSYPQ QSAHMMPHHS APFGMGGTYY AGYTPPPTPN TASAGTSSSS 250 260 270 280 290 300 AAFGWHGHPH SPFTSTSTPL SAPVAPKMRL QRSQSDAARR KRLTSTGEDE REYQSDHEAT 310 320 330 340 350 360 WDEFGDRYDN FTAGRERLQE FNGRIPPRKK KSSNSHSSSS NNPVCHTDSQ PGGTSQAESG 370 380 390 400 410 AIHGHISQQR QVERERQKAK AEKKKPQSFT WPTVVTVFVL AMGCGFFAAR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The head involution defective gene of Drosophila melanogaster functions in programmed cell death." Grether M.E., Abrams J.M., Agapite J., White K., Steller H. Genes Dev. 9:1694-1708(1995) [PubMed: 7622034] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE. Strain: Canton-S. Tissue: Eye imaginal disk.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Testis.
[5]	"Dredd, a novel effector of the apoptosis activators reaper, grim, and hid in Drosophila." Chen P., Rodriguez A., Erskine R., Thach T., Abrams J.M. Dev. Biol. 201:202-216(1998) [PubMed: 9740659] [Abstract] Cited for: FUNCTION. Tissue: Embryo.
[6]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-295, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U31226 mRNA. Translation: AAA79985.1. AE014296 Genomic DNA. Translation: AAF49270.1. AY075188 mRNA. Translation: AAL68057.1.
RefSeq	NP_524136.2.
UniGene	Dm.5624
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:20052N.
IntAct	Q24106. 6 interactions.
Genome annotation databases
Ensembl	FBgn0003997. Drosophila melanogaster. [Contig view]
GeneID	40009.
KEGG	dme:Dmel_CG5123.
NMPDR	fig|7227.3.peg.10409.
Organism-specific databases
FlyBase	FBgn0003997. W.
Phylogenomic databases
HOGENOM	Q24106.
OMA	Q24106. EDEREYQ.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-017211-MON.
Gene expression databases
ArrayExpress	Q24106.
GermOnline	CG5123. Drosophila melanogaster.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	816537.

Entry information

Entry name

HID_DROME

Accession

Primary (citable) accession number: Q24106
Secondary accession number(s): Q9VVP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project