Q24048 (ATPB2_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sodium/potassium-transporting ATPase subunit beta-2

Alternative name(s):
Protein nervana 2
Sodium/potassium-dependent ATPase subunit beta-2

Gene names

Name:	nrv2
ORF Names:	CG9261

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. Ref.6
Subunit structure	Composed of two subunits: alpha (catalytic) and beta By similarity.
Subcellular location	Membrane; Single-pass type II membrane protein Probable.
Tissue specificity	In embryos, it is expressed in the neurons of the CNS and PNS, in Garland cells and posterior spiracles. In adults, it shows a nervous system specific distribution: optic lobes, brain, thoracic ganglia and axonal pathways in the leg. Both isoforms concentrate in the adult head, isoform 2.2 being predominant. Both isoforms are weakly expressed in the thorax and very poorly expressed in the abdomen. Ref.5 Ref.6
Developmental stage	Expression in embryos is first seen 12 hours after oviposition, peaks at 24 hours and decreases to a low level by 48 hours. Low levels are seen during larval and early pupal development. Levels increase during late pupae to maximal at the adult stage. Ref.5
Sequence similarities	Belongs to the X(+)/potassium ATPases subunit beta family.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Sodium transport Sodium/potassium transport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Potassium Sodium
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro establishment of glial blood-brain barrier Inferred from mutant phenotype. Source: FlyBase regulation of tube diameter, open tracheal system Inferred from mutant phenotype. Source: FlyBase regulation of tube length, open tracheal system Inferred from mutant phenotype. Source: FlyBase septate junction assembly Inferred from mutant phenotype. Source: FlyBase
Cellular component	septate junction Inferred from direct assay. Source: FlyBase sodium:potassium-exchanging ATPase complex Inferred by curator Ref.6. Source: FlyBase
Molecular function	sodium:potassium-exchanging ATPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 2.2 (identifier: Q24048-1)

Also known as: C; F;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2.1 (identifier: Q24048-2)

Also known as: A; D;

The sequence of this isoform differs from the canonical sequence as follows:
     1-46: MPTITEDCID...DNSYFGRSMD → MSKPVPMSPS...DGTVMGRDRS

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	Sodium/potassium-transporting ATPase subunit beta-2		PRO_0000219121
Regions
	Topological domain	1 – 50	50	Cytoplasmic Potential
	Transmembrane	51 – 71	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	72 – 323	252	Extracellular Potential
Amino acid modifications
	Glycosylation	180	1	N-linked (GlcNAc...) Potential
	Glycosylation	206	1	N-linked (GlcNAc...) Ref.7
	Disulfide bond	153 ↔ 165		By similarity
	Disulfide bond	175 ↔ 189		By similarity
	Disulfide bond	241 ↔ 298		By similarity
Natural variations
	Alternative sequence	1 – 46	46	MPTIT…GRSMD → MSKPVPMSPSFVDEDLHNLR KPKPFKLGQFLYNTEDGTVM GRDRS in isoform 2.1.		VSP_000350
Experimental info
	Sequence conflict	162	1	G → A in AAC46609. Ref.1
	Sequence conflict	162	1	G → A in AAC46610. Ref.1
	Sequence conflict	307	1	H → R in AAC46609. Ref.1
	Sequence conflict	307	1	H → R in AAC46610. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 2.2 (C) (F) [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 8926A9D1FC48D6EF	FASTA	323	37,283
	10 20 30 40 50 60 MPTITEDCID GFQQYYSRPP ERPKKKSLKQ MVYDSEDNSY FGRSMDSWAK IGIFYVAFYG 70 80 90 100 110 120 VLAALVAICM WAFFQTLDPR IPKWTLDRSL IGTNPGLGFR PLPPVDNVES TLIWYKGTQH 130 140 150 160 170 180 ENYKHWTDSL DDFLAVYKVP GLTPGRGQNI YNCDYNQPPP KGQVCDVDIK TWSPCTKENN 190 200 210 220 230 240 YSYHKSAPCI FLKLNKIYGW IPEYYNRSND LPANMPASLK TYIAEVEKTQ PEKLNTIWVS 250 260 270 280 290 300 CEGENPADQE NIGAVNYLPI RGFPGYFYPY QNSEGYLSPL VAVHFQRPKR GIIINVECRA 310 320 WARNIIHDRK ERIGSVHYEL LID
	Isoform 2.1 (A) (D) [UniParc]. Checksum: 040ECF8D0867E32F	FASTA	322	36,967
	10 20 30 40 50 60 MSKPVPMSPS FVDEDLHNLR KPKPFKLGQF LYNTEDGTVM GRDRSSWAKI GIFYVAFYGV 70 80 90 100 110 120 LAALVAICMW AFFQTLDPRI PKWTLDRSLI GTNPGLGFRP LPPVDNVEST LIWYKGTQHE 130 140 150 160 170 180 NYKHWTDSLD DFLAVYKVPG LTPGRGQNIY NCDYNQPPPK GQVCDVDIKT WSPCTKENNY 190 200 210 220 230 240 SYHKSAPCIF LKLNKIYGWI PEYYNRSNDL PANMPASLKT YIAEVEKTQP EKLNTIWVSC 250 260 270 280 290 300 EGENPADQEN IGAVNYLPIR GFPGYFYPYQ NSEGYLSPLV AVHFQRPKRG IIINVECRAW 310 320 ARNIIHDRKE RIGSVHYELL ID

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two Drosophila nervous system antigens, Nervana 1 and 2, are homologous to the beta subunit of Na+,K(+)-ATPase." Sun B., Salvaterra P.M. Proc. Natl. Acad. Sci. U.S.A. 92:5396-5400(1995) [PubMed: 7777518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2.1 AND 2.2). Strain: Canton-S. Tissue: Embryo.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2.2). Strain: Berkeley. Tissue: Head.
[5]	"Characterization of nervana, a Drosophila melanogaster neuron-specific glycoprotein antigen recognized by anti-horseradish peroxidase antibodies." Sun B., Salvaterra P.M. J. Neurochem. 65:434-443(1995) [PubMed: 7540667] [Abstract] Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY. Strain: Canton-S. Tissue: Head.
[6]	"Functional analysis and tissue-specific expression of Drosophila Na+,K+-ATPase subunits." Sun B., Wang W., Salvaterra P.M. J. Neurochem. 71:142-151(1998) [PubMed: 9648860] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[7]	"Identification of N-glycosylated proteins from the central nervous system of Drosophila melanogaster." Koles K., Lim J.-M., Aoki K., Porterfield M., Tiemeyer M., Wells L., Panin V. Glycobiology 17:1388-1403(2007) [PubMed: 17893096] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-206, MASS SPECTROMETRY. Strain: Oregon-R. Tissue: Head.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U22439 mRNA. Translation: AAC46609.1. U22440 mRNA. Translation: AAC46610.1. AE014134 Genomic DNA. Translation: AAF52438.2. AE014134 Genomic DNA. Translation: AAN10600.1. AY060289 mRNA. Translation: AAL25328.1.
RefSeq	NP_001014475.1. NM_001014475.1. NP_001014476.1. NM_001014476.1. NP_477168.1. NM_057820.3. NP_477169.1. NM_057821.3. NP_723216.1. NM_164711.1.
UniGene	Dm.4056.
3D structure databases
ProteinModelPortal	Q24048.
SMR	Q24048. Positions 34-323.
ModBase	Search...
Protein-protein interaction databases
STRING	Q24048.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0079381; FBpp0079009; FBgn0015777. FBtr0100382; FBpp0099794; FBgn0015777.
GeneID	33953.
KEGG	dme:Dmel_CG9261.
NMPDR	fig|7227.3.peg.1131.
Organism-specific databases
CTD	33953.
FlyBase	FBgn0015777. nrv2.
Phylogenomic databases
eggNOG	inNOG07024.
GeneTree	EMGT00050000007260.
InParanoid	Q24048.
OMA	DLKTYLW.
OrthoDB	EOG4JM65G.
PhylomeDB	Q24048.
Gene expression databases
ArrayExpress	Q24048.
Bgee	Q24048.
GermOnline	CG9261. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000402. ATPase_P-typ_cation-exchng_bsu. [Graphical view]
KO	K01540.
PANTHER	PTHR11523. ATPase_H_Na/K_b. 1 hit.
Pfam	PF00287. Na_K-ATPase. 1 hit. [Graphical view]
PROSITE	PS00390. ATPASE_NA_K_BETA_1. False negative. PS00391. ATPASE_NA_K_BETA_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	786105.

Entry information

Entry name

ATPB2_DROME

Accession

Primary (citable) accession number: Q24048
Secondary accession number(s): Q24047, Q9VM84, Q9VM85

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project