Q24046 (ATPB1_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sodium/potassium-transporting ATPase subunit beta-1

Alternative name(s):
Protein nervana 1
Sodium/potassium-dependent ATPase subunit beta-1

Gene names

Name:	nrv1
ORF Names:	CG9258

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. Ref.6
Subunit structure	Composed of two subunits: alpha (catalytic) and beta By similarity. Interacts with nkain. Ref.8
Subcellular location	Membrane; Single-pass type II membrane protein Probable.
Tissue specificity	In embryos, it is expressed in the neurons of the CNS and PNS, in Garland cells and posterior spiracles. In adults, it is concentrated in the thorax and abdomen (muscle tissue, digestive system and Malpighian tubules) and weakly expressed in the head. Expression is diffuse in the nervous system. Ref.5 Ref.6
Developmental stage	Expression in embryos is first seen 12 hours after oviposition, peaks at 24 hours and decreases to a low level by 48 hours. Low levels are seen during larval and early pupal development. Levels increase during late pupae to maximal at the adult stage. Ref.5
Sequence similarities	Belongs to the X(+)/potassium ATPases subunit beta family.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Sodium transport Sodium/potassium transport Transport
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Potassium Sodium
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	sodium:potassium-exchanging ATPase complex Inferred by curator Ref.6. Source: FlyBase
Molecular function	protein binding Inferred from physical interaction Ref.8. Source: UniProtKB sodium:potassium-exchanging ATPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Sodium/potassium-transporting ATPase subunit beta-1		PRO_0000219120
Regions
	Topological domain	1 – 45	45	Cytoplasmic Potential
	Transmembrane	46 – 66	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	67 – 309	243	Extracellular Potential
Amino acid modifications
	Glycosylation	133	1	N-linked (GlcNAc...) Ref.7
	Glycosylation	211	1	N-linked (GlcNAc...) Ref.7
	Disulfide bond	143 ↔ 155		By similarity
	Disulfide bond	165 ↔ 179		By similarity
	Disulfide bond	225 ↔ 282		By similarity
Experimental info
	Sequence conflict	205	1	D → N in AAM52659. Ref.4
	Sequence conflict	219 – 220	2	QQ → HE in AAC46608. Ref.1
	Sequence conflict	226	1	N → F in AAC46608. Ref.1
	Sequence conflict	295	1	V → A in AAC46608. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q24046 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 35EA43910B86D84F	FASTA	309	35,309
	10 20 30 40 50 60 MSKNNGKGAK GEFEFPQPAK KQTFSEMIYN PQEGTFFGRT GKSWSQLLLF YTIFYIVLAA 70 80 90 100 110 120 LFTICMQGLL STISDTEPKW KLQDSLIGTN PGLGFRPLSE QTERGSVIAF DGKKPAESDY 130 140 150 160 170 180 WIELIDDFLR DYNHTEGRDM KHCGFGQVLE PTDVCVVNTD LFGGCSKANN YGYKTNQPCI 190 200 210 220 230 240 FLKLNKIFGW IPEVYDKEEK DMPDDLKKVI NETKTEERQQ VWVSCNGHLG KDKENFQNIR 250 260 270 280 290 300 YFPSQGFPSY YYPFLNQPGY LSPLVAVQFN SPPKGQMLDV ECRAWAKNIQ YSGSVRDRKG SVTFQILLD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two Drosophila nervous system antigens, Nervana 1 and 2, are homologous to the beta subunit of Na+,K(+)-ATPase." Sun B., Salvaterra P.M. Proc. Natl. Acad. Sci. U.S.A. 92:5396-5400(1995) [PubMed: 7777518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Canton-S. Tissue: Embryo.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"Characterization of nervana, a Drosophila melanogaster neuron-specific glycoprotein antigen recognized by anti-horseradish peroxidase antibodies." Sun B., Salvaterra P.M. J. Neurochem. 65:434-443(1995) [PubMed: 7540667] [Abstract] Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY. Strain: Canton-S. Tissue: Head.
[6]	"Functional analysis and tissue-specific expression of Drosophila Na+,K+-ATPase subunits." Sun B., Wang W., Salvaterra P.M. J. Neurochem. 71:142-151(1998) [PubMed: 9648860] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[7]	"Identification of N-glycosylated proteins from the central nervous system of Drosophila melanogaster." Koles K., Lim J.-M., Aoki K., Porterfield M., Tiemeyer M., Wells L., Panin V. Glycobiology 17:1388-1403(2007) [PubMed: 17893096] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-133 AND ASN-211, MASS SPECTROMETRY. Strain: Oregon-R. Tissue: Head.
[8]	"A novel family of transmembrane proteins interacting with beta subunits of the Na,K-ATPase." Gorokhova S., Bibert S., Geering K., Heintz N. Hum. Mol. Genet. 16:2394-2410(2007) [PubMed: 17606467] [Abstract] Cited for: INTERACTION WITH NKAIN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U22438 mRNA. Translation: AAC46608.1. AE014134 Genomic DNA. Translation: AAF52437.1. AY122147 mRNA. Translation: AAM52659.1.
RefSeq	NP_477167.1. NM_057819.3.
UniGene	Dm.4821.
3D structure databases
ProteinModelPortal	Q24046.
SMR	Q24046. Positions 30-309.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-20098N.
MINT	MINT-1012223.
STRING	Q24046.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0079384; FBpp0079012; FBgn0015776.
GeneID	33952.
KEGG	dme:Dmel_CG9258.
NMPDR	fig|7227.3.peg.1130.
Organism-specific databases
CTD	33952.
FlyBase	FBgn0015776. nrv1.
Phylogenomic databases
eggNOG	inNOG07024.
GeneTree	EMGT00050000007260.
InParanoid	Q24046.
OMA	KPAESDY.
OrthoDB	EOG4DNCM0.
PhylomeDB	Q24046.
Gene expression databases
ArrayExpress	Q24046.
Bgee	Q24046.
GermOnline	CG9258. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000402. ATPase_P-typ_cation-exchng_bsu. [Graphical view]
KO	K01540.
PANTHER	PTHR11523. ATPase_H_Na/K_b. 1 hit.
Pfam	PF00287. Na_K-ATPase. 1 hit. [Graphical view]
PROSITE	PS00390. ATPASE_NA_K_BETA_1. 1 hit. PS00391. ATPASE_NA_K_BETA_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	786100.

Entry information

Entry name

ATPB1_DROME

Accession

Primary (citable) accession number: Q24046
Secondary accession number(s): Q8MR34, Q9VM86

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project