Q24025 (SOG_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dorsal-ventral patterning protein Sog

Alternative name(s):
Short gastrulation protein

Gene names

Name:	sog
ORF Names:	CG9224

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	1038 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Putative negative growth factor. Antagonist of dpp, a protein involved in patterning the dorsal region and in the development of the neuroectoderm; dpp inhibition is enhanced by tsg. Required for establishment of a narrow stripe of peak levels of BMP signaling in the dorsal midline of early embryos, that will give rise to the amnioserosa. Ref.1 Ref.5
Subunit structure	Component of a complex composed of dpp, sog and tsg.
Subcellular location	Membrane; Single-pass type II membrane protein Potential.
Tissue specificity	Abuts the dorsal dpp-expressing cells in a lateral stripe 14-16 cells wide. Later in embryogenesis it is expressed in neuroectoderm and in the endoderm spaced along the anterior-posterior axis of the developing gut. Ref.1
Developmental stage	Embryogenesis. Ref.1
Sequence similarities	Belongs to the chordin family. Contains 4 CHRD domains. Contains 4 VWFC domains.

Ontologies

Keywords
Biological process	Growth regulation
Cellular component	Membrane
Domain	Repeat Signal-anchor Transmembrane Transmembrane helix
Molecular function	Developmental protein Growth factor
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	BMP signaling pathway Non-traceable author statement PubMed 11290316. Source: FlyBase amnioserosa formation Traceable author statement PubMed 1765005. Source: FlyBase ectoderm development Non-traceable author statement PubMed 9712300. Source: FlyBase imaginal disc-derived wing vein morphogenesis Inferred from mutant phenotype PubMed 15766758PubMed 15872004. Source: FlyBase maternal specification of dorsal/ventral axis, oocyte, soma encoded Inferred from mutant phenotype PubMed 16781701. Source: FlyBase negative regulation of transforming growth factor beta receptor signaling pathway Traceable author statement PubMed 11700289PubMed 15380237. Source: FlyBase positive regulation of transforming growth factor beta receptor signaling pathway Traceable author statement PubMed 11700289. Source: FlyBase posterior Malpighian tubule development Inferred from mutant phenotype PubMed 17190812. Source: FlyBase regulation of BMP signaling pathway Traceable author statement PubMed 11532914. Source: FlyBase regulation of growth Inferred from electronic annotation. Source: UniProtKB-KW ring gland development Inferred from mutant phenotype PubMed 15385159. Source: FlyBase terminal region determination Inferred from genetic interaction PubMed 1905816. Source: FlyBase torso signaling pathway Inferred from genetic interaction PubMed 1905816. Source: FlyBase
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from sequence or structural similarity Ref.1. Source: FlyBase
Molecular_function	collagen binding Inferred from direct assay PubMed 22733779. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1038	1038	Dorsal-ventral patterning protein Sog		PRO_0000219089
Regions
	Topological domain	1 – 53	53	Cytoplasmic Potential
	Transmembrane	54 – 74	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	75 – 1038	964	Extracellular Potential
	Domain	100 – 175	76	VWFC 1
	Domain	197 – 337	141	CHRD 1
	Domain	339 – 471	133	CHRD 2
	Domain	474 – 588	115	CHRD 3
	Domain	592 – 713	122	CHRD 4
	Domain	742 – 804	63	VWFC 2
	Domain	830 – 899	70	VWFC 3
	Domain	939 – 1020	82	VWFC 4
Amino acid modifications
	Glycosylation	179	1	N-linked (GlcNAc...) Potential
	Glycosylation	287	1	N-linked (GlcNAc...) Potential
	Glycosylation	520	1	N-linked (GlcNAc...) Potential
	Glycosylation	666	1	N-linked (GlcNAc...) Potential
	Glycosylation	752	1	N-linked (GlcNAc...) Potential
	Glycosylation	821	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q24025 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B0E833AFD79A9037	FASTA	1,038	115,515
	10 20 30 40 50 60 MANKLRKSNA IEWATATGTV PLLERSCCHS EDAALEPQAS KTSHREQAPI LRHLSQLSHL 70 80 90 100 110 120 LIIAGLLIVC LAGVTEGRRH APLMFEESDT GRRSNRPAVT ECQFGKVLRE LGSTWYADLG 130 140 150 160 170 180 PPFGVMYCIK CECVAIPKKR RIVARVQCRN IKNECPPAKC DDPISLPGKC CKTCPGDRND 190 200 210 220 230 240 TDVALDVPVP NEEEERNMKH YAALLTGRTS YFLKGEEMKS MYTTYNPQNV VATARFLFHK 250 260 270 280 290 300 KNLYYSFYTS SRIGRPRAIQ FVDDAGVILE EHQLETTLAG TLSVYQNATG KICGVWRRVP 310 320 330 340 350 360 RDYKRILRDD RLHVVLLWGN KQQAELALAG KVAKYTALQT ELFSSLLEAP LPDGKTDPQL 370 380 390 400 410 420 AGAGGTAIVS TSSGAASSMH LTLVFNGVFG AEEYADAALS VKIELAERKE VIFDEIPRVR 430 440 450 460 470 480 KPSAEINVLE LSSPISIQNL RLMSRGKLLL TVESKKYPHL RIQGHIVTRA SCEIFQTLLA 490 500 510 520 530 540 PHSAESSTKS SGLAWVYLNT DGSLAYNIET EHVNTRDRPN ISLIEEQGKR KAKLEDLTPS 550 560 570 580 590 600 FNFNQAIGSV EKLGPKVLES LYAGELGVNV ATEHETSLIR GRLVPRPVAD ARDSAEPILL 610 620 630 640 650 660 KRQEHTDAQN PHAVGMAWMS IDNECNLHYE VTLNGVPAQD LQLYLEEKPI EAIGAPVTRK 670 680 690 700 710 720 LLEEFNGSYL EGFFLSMPSA ELIKLEMSVC YLEVHSKHSK QLLLRGKLKS TKVPGHCFPV 730 740 750 760 770 780 YTDNNVPVPG DHNDNHLVNG ETKCFHSGRF YNESEQWRSA QDSCQMCACL RGQSSCEVIK 790 800 810 820 830 840 CPALKCKSTE QLLQRDGECC PSCVPKKEAA DYSAQSSPAT NATDLLQQRR GCRLGEQFHP 850 860 870 880 890 900 AGASWHPFLP PNGFDTCTTC SCDPLTLEIR CPRLVCPPLQ CSEKLAYRPD KKACCKICPE 910 920 930 940 950 960 GKQSSSNGHK TTPNNPNVLQ DQAMQRSPSH SAEEVLANGG CKVVNKVYEN GQEWHPILMS 970 980 990 1000 1010 1020 HGEQKCIKCR CKDSKVNCDR KRCSRSTCQQ QTRVTSKRRL FEKPDAAAPA IDECCSTQCR 1030 RSRRHHKRQP HHQQRSSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dorsal-ventral patterning of the Drosophila embryo depends on a putative negative growth factor encoded by the short gastrulation gene." Francois V., Solloway M., O'Neill J.W., Emery J., Bier E. Genes Dev. 8:2602-2616(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley.
[5]	"Twisted gastrulation is a conserved extracellular BMP antagonist." Ross J.J., Shimmi O., Vilmos P., Petryk A., Kim H., Gaudenz K., Hermanson S., Ekker S.C., O'Connor M.B., Marsh J.L. Nature 410:479-483(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH DPP AND TSG.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18774 mRNA. Translation: AAA89117.1. AE014298 Genomic DNA. Translation: AAF48481.1. BT053679 mRNA. Translation: ACK77594.1.
PIR	T13177.
RefSeq	NP_001259576.1. NM_001272647.1. NP_001259578.1. NM_001272649.1. NP_476736.1. NM_057388.3.
UniGene	Dm.3944.
3D structure databases
ProteinModelPortal	Q24025.
SMR	Q24025. Positions 101-177, 738-807, 828-903, 936-993.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-20760N.
IntAct	Q24025. 3 interactions.
MINT	MINT-299642.
Proteomic databases
PRIDE	Q24025.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0074063; FBpp0073879; FBgn0003463. FBtr0331760; FBpp0304148; FBgn0003463. FBtr0331762; FBpp0304150; FBgn0003463.
GeneID	32498.
KEGG	dme:Dmel_CG9224.
Organism-specific databases
CTD	32498.
FlyBase	FBgn0003463. sog.
Phylogenomic databases
eggNOG	NOG85639.
GeneTree	ENSGT00700000104447.
InParanoid	Q24025.
KO	K04657.
OMA	GARWAPN.
OrthoDB	EOG4K0P37.
PhylomeDB	Q24025.
Gene expression databases
Bgee	Q24025.
GermOnline	CG9224. Drosophila melanogaster.
Family and domain databases
InterPro	IPR016353. Chordin. IPR010895. CHRD. IPR001007. VWF_C. [Graphical view]
Pfam	PF07452. CHRD. 4 hits. PF00093. VWC. 4 hits. [Graphical view]
PIRSF	PIRSF002496. Chordin. 1 hit.
SMART	SM00754. CHRD. 4 hits. SM00214. VWC. 3 hits. [Graphical view]
PROSITE	PS50933. CHRD. 4 hits. PS01208. VWFC_1. 2 hits. PS50184. VWFC_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	32498.
NextBio	778773.

Entry information

Entry name

SOG_DROME

Accession

Primary (citable) accession number: Q24025
Secondary accession number(s): B7FNI9, Q9VXS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project