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Q23W20

- Q23W20_TETTS

UniProt

Q23W20 - Q23W20_TETTS

Protein

Isocitrate dehydrogenase [NADP]

Gene

TTHERM_00794110

Organism
Tetrahymena thermophila (strain SB210)
Status
Unreviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 3 (16 Apr 2014)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.UniRule annotation

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei80 – 801SubstrateUniRule annotation
    Binding sitei85 – 851NADP
    Binding sitei112 – 1121SubstrateUniRule annotation
    Binding sitei126 – 1261Substrate
    Binding sitei135 – 1351SubstrateUniRule annotation
    Sitei142 – 1421Critical for catalysisUniRule annotation
    Binding sitei158 – 1581Substrate
    Sitei214 – 2141Critical for catalysisUniRule annotation
    Metal bindingi254 – 2541Magnesium or manganeseUniRule annotation
    Binding sitei262 – 2621NADP
    Metal bindingi277 – 2771Magnesium or manganeseUniRule annotation
    Binding sitei329 – 3291NADP; via amide nitrogen and carbonyl oxygen

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi78 – 803NADP
    Nucleotide bindingi311 – 3166NADP

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. isocitrate metabolic process Source: InterPro
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    OxidoreductaseUniRule annotation

    Keywords - Biological processi

    Tricarboxylic acid cycleUniRule annotation

    Keywords - Ligandi

    MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, NADPUniRule annotation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NADP]UniRule annotation (EC:1.1.1.42UniRule annotation)
    Gene namesi
    ORF Names:TTHERM_00794110Imported
    OrganismiTetrahymena thermophila (strain SB210)Imported
    Taxonomic identifieri312017 [NCBI]
    Taxonomic lineageiEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaHymenostomatidaTetrahymeninaTetrahymenidaeTetrahymena
    ProteomesiUP000009168: Unassembled WGS sequence

    Interactioni

    Protein-protein interaction databases

    STRINGi5911.XP_001020930.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni97 – 1037Substrate bindingUniRule annotation
    Regioni143 – 1497Substrate binding

    Sequence similaritiesi

    Belongs to the isocitrate and isopropylmalate dehydrogenases family.UniRule annotation

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11822. PTHR11822. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q23W20-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAELQKIKVT NPVTELDGDE MTRVIWQLIK DKLILPYLDL NIKYFDLGME    50
    YRDQTDDKVT LDAAQSIVET KVGIKCATIT PDEARVKEFN LKKMWKSPNG 100
    TIRNHIGGTV FREPILMKNV PRLVPAWTKP IVIGRHAFGD QYRATDFKVE 150
    EAGTFEIVFT PKNGGEKKVM KVFDYPAGGV GMGMYNTDES ITEFAHSSFK 200
    YALDRKMPLY LSTKNTILKA YDGKFKDIFQ AIYDKQYKEE FTKAGIWYEH 250
    RLIDDMVAYM MKSEGGFMWA CKNYDGDVQS DCLAQGFGSL GLMTSVLVGP 300
    NDIIEAEAAH GTVTRHFRQH QKGQETSTNS IASIFAWTQG LLHRAKLDNN 350
    KELDLFCKTL ESSVIETVET GFMTKDLAIC IQGSNDVPRS KYLTTQEFID 400
    KVAETLRKNL VAAKSNL 417
    Length:417
    Mass (Da):47,211
    Last modified:April 16, 2014 - v3
    Checksum:i91ED2EC90E256C3B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GG662609 Genomic DNA. Translation: EAS00685.3.
    UniGeneiTth.2460.

    Genome annotation databases

    EnsemblProtistsiEAS00685; EAS00685; TTHERM_00794110.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GG662609 Genomic DNA. Translation: EAS00685.3 .
    UniGenei Tth.2460.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5911.XP_001020930.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi EAS00685 ; EAS00685 ; TTHERM_00794110 .

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11822. PTHR11822. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a model eukaryote."
      Eisen J.A., Coyne R.S., Wu M., Wu D., Thiagarajan M., Wortman J.R., Badger J.H., Ren Q., Amedeo P., Jones K.M., Tallon L.J., Delcher A.L., Salzberg S.L., Silva J.C., Haas B.J., Majoros W.H., Farzad M., Carlton J.M.
      , Smith R.K. Jr., Garg J., Pearlman R.E., Karrer K.M., Sun L., Manning G., Elde N.C., Turkewitz A.P., Asai D.J., Wilkes D.E., Wang Y., Cai H., Collins K., Stewart B.A., Lee S.R., Wilamowska K., Weinberg Z., Ruzzo W.L., Wloga D., Gaertig J., Frankel J., Tsao C.-C., Gorovsky M.A., Keeling P.J., Waller R.F., Patron N.J., Cherry J.M., Stover N.A., Krieger C.J., del Toro C., Ryder H.F., Williamson S.C., Barbeau R.A., Hamilton E.P., Orias E.
      PLoS Biol. 4:1620-1642(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SB210Imported.

    Entry informationi

    Entry nameiQ23W20_TETTS
    AccessioniPrimary (citable) accession number: Q23W20
    Entry historyi
    Integrated into UniProtKB/TrEMBL: April 18, 2006
    Last sequence update: April 16, 2014
    Last modified: October 1, 2014
    This is version 65 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3