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Q23K14

- Q23K14_TETTS

UniProt

Q23K14 - Q23K14_TETTS

Protein

Isocitrate dehydrogenase [NADP]

Gene

TTHERM_00196200

Organism
Tetrahymena thermophila (strain SB210)
Status
Unreviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (18 Apr 2006)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.UniRule annotation

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei93 – 931SubstrateUniRule annotation
    Binding sitei98 – 981NADPUniRule annotation
    Binding sitei125 – 1251SubstrateUniRule annotation
    Binding sitei148 – 1481SubstrateUniRule annotation
    Sitei155 – 1551Critical for catalysisUniRule annotation
    Sitei228 – 2281Critical for catalysisUniRule annotation
    Metal bindingi268 – 2681Magnesium or manganese
    Binding sitei276 – 2761NADPUniRule annotation
    Metal bindingi291 – 2911Magnesium or manganese
    Binding sitei343 – 3431NADP; via amide nitrogen and carbonyl oxygenUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi91 – 933NADPUniRule annotation
    Nucleotide bindingi325 – 3306NADPUniRule annotation

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. isocitrate metabolic process Source: InterPro
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    OxidoreductaseUniRule annotation

    Keywords - Biological processi

    Tricarboxylic acid cycleUniRule annotation

    Keywords - Ligandi

    MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, NADPUniRule annotation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NADP]UniRule annotation (EC:1.1.1.42UniRule annotation)
    Gene namesi
    ORF Names:TTHERM_00196200Imported
    OrganismiTetrahymena thermophila (strain SB210)Imported
    Taxonomic identifieri312017 [NCBI]
    Taxonomic lineageiEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaHymenostomatidaTetrahymeninaTetrahymenidaeTetrahymena
    ProteomesiUP000009168: Unassembled WGS sequence

    Interactioni

    Protein-protein interaction databases

    STRINGi5911.XP_001017274.

    Structurei

    3D structure databases

    ProteinModelPortaliQ23K14.
    SMRiQ23K14. Positions 16-419.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni110 – 1167Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the isocitrate and isopropylmalate dehydrogenases family.UniRule annotation

    Phylogenomic databases

    KOiK00031.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11822. PTHR11822. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q23K14-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMNQTIKNAA SKYKYAGNKI NVKNPIVDLD GDEMTRIIWK MIKDKLILPF    50
    ISADIRYYDL SIENRDATND QVTFDAAYAI REHKVGIKCA TITADEARVK 100
    EFKLKKMWPS PNGTIRNILD GTVFREPIVC QNIPRLVPGW TKPIIIGRHS 150
    FGDQYKCTNF KVQGPGKLEI SFKPQDGGEE IRKTVFEFSG KGGVAMGMYN 200
    TIESIESFAN SCFKYALNRQ YPLYFSSKNT ILKQYDGLFK DIFEELYTTK 250
    YKQQFEKSGL WYEHRLIDDM VAQVLKGDGG IVWACKNYDG DVQSDIIAQG 300
    YGSLGMMTSE LVNNDNVYEA EAAHGTVTRH YRQHQKGQET STNSVASIYA 350
    WSRGLRHRAK LDNNQDLLTF SQCIEESVLD CISNGKMTKD LAILVYGQDN 400
    IKGKYLNTEE FIDAVAYRLK SQIHIEK 427
    Length:427
    Mass (Da):48,673
    Last modified:April 18, 2006 - v1
    Checksum:iBF378B6922FB9CC4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GG662673 Genomic DNA. Translation: EAR97029.1.
    RefSeqiXP_001017274.1. XM_001017274.1.
    UniGeneiTth.28000.

    Genome annotation databases

    EnsemblProtistsiEAR97029; EAR97029; TTHERM_00196200.
    GeneIDi7825818.
    KEGGitet:TTHERM_00196200.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GG662673 Genomic DNA. Translation: EAR97029.1 .
    RefSeqi XP_001017274.1. XM_001017274.1.
    UniGenei Tth.28000.

    3D structure databases

    ProteinModelPortali Q23K14.
    SMRi Q23K14. Positions 16-419.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5911.XP_001017274.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi EAR97029 ; EAR97029 ; TTHERM_00196200 .
    GeneIDi 7825818.
    KEGGi tet:TTHERM_00196200.

    Phylogenomic databases

    KOi K00031.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11822. PTHR11822. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a model eukaryote."
      Eisen J.A., Coyne R.S., Wu M., Wu D., Thiagarajan M., Wortman J.R., Badger J.H., Ren Q., Amedeo P., Jones K.M., Tallon L.J., Delcher A.L., Salzberg S.L., Silva J.C., Haas B.J., Majoros W.H., Farzad M., Carlton J.M.
      , Smith R.K. Jr., Garg J., Pearlman R.E., Karrer K.M., Sun L., Manning G., Elde N.C., Turkewitz A.P., Asai D.J., Wilkes D.E., Wang Y., Cai H., Collins K., Stewart B.A., Lee S.R., Wilamowska K., Weinberg Z., Ruzzo W.L., Wloga D., Gaertig J., Frankel J., Tsao C.-C., Gorovsky M.A., Keeling P.J., Waller R.F., Patron N.J., Cherry J.M., Stover N.A., Krieger C.J., del Toro C., Ryder H.F., Williamson S.C., Barbeau R.A., Hamilton E.P., Orias E.
      PLoS Biol. 4:1620-1642(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SB210Imported.

    Entry informationi

    Entry nameiQ23K14_TETTS
    AccessioniPrimary (citable) accession number: Q23K14
    Entry historyi
    Integrated into UniProtKB/TrEMBL: April 18, 2006
    Last sequence update: April 18, 2006
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3