Q23985 (DTX_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein deltex

Gene names

Name:	dx
ORF Names:	CG3929

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	738 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Mainly acts as a positive regulator of Notch, but it may also act as a negative regulator, depending on the developmental and cell context. Mediates the antineural activity of Notch. May function as an ubiquitin ligase protein in the Notch pathway. Ref.7 Ref.8 Ref.9
Subunit structure	Homomultimer; the oligomerization is required for its function. Interacts with the ankyrin repeats of Notch. Ref.7 Ref.9
Subcellular location	Cytoplasm Ref.1.
Tissue specificity	Ubiquitous. Expressed at low levels throughout embryogenesis and in larvae. Ref.1
Domain	The WWE domains are thought to mediate some protein-protein interaction, and are frequently found in ubiquitin ligases By similarity.
Sequence similarities	Belongs to the Deltex family. Contains 1 RING-type zinc finger. Contains 2 WWE domains.
Sequence caution	The sequence AAO39479.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Notch signaling pathway
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Domain	Repeat SH3-binding Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Notch signaling pathway Inferred from genetic interaction. Source: FlyBase Wnt receptor signaling pathway Inferred from mutant phenotype Ref.8. Source: UniProtKB negative regulation of Notch signaling pathway Inferred from direct assay Ref.9Ref.7. Source: UniProtKB positive regulation of Notch signaling pathway Inferred from direct assay Ref.9Ref.7. Source: UniProtKB
Cellular component	cytoplasm Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	Notch binding Inferred from physical interaction Ref.9. Source: UniProtKB SH3 domain binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 738

738

Protein deltex

PRO_0000219079

Regions

Domain

35 – 118

84

WWE 1

Domain

119 – 201

83

WWE 2

Zinc finger

548 – 603

56

RING-type

Region

1 – 304

304

Interaction with Notch

Motif

480 – 484

5

SH3-binding Potential

Compositional bias

251 – 303

53

Gln/His-rich (OPA repeat)

Compositional bias

332 – 472

141

Ser-rich

Compositional bias

489 – 514

26

Poly-Gln (OPA repeat)

Amino acid modifications

Modified residue

425

1

Phosphoserine Ref.10

Natural variations

Natural variant

511

1

Q → L in strain: ZW141. Ref.6

Natural variant

514

1

Q → QQ in strain: ZW122. Ref.6

Experimental info

Mutagenesis

476 – 484

9

Missing: Dominant-negative mutant that inhibits Notch signaling. Ref.9

Mutagenesis

571

1

H → A: Loss of function. Abolishes homomultimerization; when associated with A-574. Ref.9

Mutagenesis

574

1

H → A: Loss of function. Abolishes homomultimerization; when associated with A-571. Ref.9

Sequence conflict

107 – 108

2

AG → R in AAA18501. Ref.1

Sequence conflict

120

1

F → L in AAA18501. Ref.1

Sequence conflict

188 – 189

2

QL → HV in AAA18501. Ref.1

Sequence conflict

268

1

H → L in AAA18501. Ref.1

Sequence conflict

444

1

R → S in AAO39479. Ref.5

Sequence conflict

468

1

L → V in AAA18501. Ref.1

Secondary structure

1

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738

Helix Strand Turn

Details...

Beta strand

45 – 53

9

Beta strand

56 – 59

4

Helix

62 – 73

12

Beta strand

78 – 80

3

Helix

81 – 83

3

Beta strand

92 – 94

3

Turn

95 – 98

4

Beta strand

99 – 101

3

Beta strand

115 – 121

7

Helix

126 – 129

4

Beta strand

131 – 136

6

Beta strand

138 – 141

4

Beta strand

144 – 148

5

Helix

151 – 162

12

Beta strand

166 – 169

4

Helix

170 – 173

4

Beta strand

179 – 182

4

Turn

183 – 186

4

Beta strand

187 – 190

4

Turn

191 – 193

3

Beta strand

196 – 203

8

Sequences

	Sequence		Length	Mass (Da)
	Q23985 [UniParc]. Last modified August 15, 2003. Version 2. Checksum: 7B1CF29E024D26AF	FASTA	738	82,186
	10 20 30 40 50 60 MASSAGSAAS GSVVPGGGGS AASSCATMAL STAGSGGPPV NHAHAVCVWE FESRGKWLPY 70 80 90 100 110 120 SPAVSQHLER AHAKKLTRVM LSDADPSLEQ YYVNVRTMTQ ESEAETAGSG LLTIGVRRMF 130 140 150 160 170 180 YAPSSPAGKG TKWEWSGGSA DSNNDWRPYN MHVQCIIEDA WARGEQTLDL CNTHIGLPYT 190 200 210 220 230 240 INFCNLTQLR QPSGPMRSIR RTQQAPYPLV KLTPQQANQL KSNSASVSSQ YNTLPKLGDT 250 260 270 280 290 300 KSLHRVPMTR QQHPLPTSHQ VQQQQHQHQH QQQQQQQHHH QHQQQQHQQQ QQHQMQHHQI 310 320 330 340 350 360 HHQTAPRKPP KKHSEISTTN LRQILNNLNI FSSSTKHQSN MSTAASASSS SSSASLHHAN 370 380 390 400 410 420 HLSHAHFSHA KNMLTASMNS HHSRCSEGSL QSQRSSRMGS HRSRSRTRTS DTDTNSVKSH 430 440 450 460 470 480 RRRPSVDTVS TYLSHESKES LRSRNFAISV NDLLDCSLGS DEVFVPSLPP SSLGERAPVP 490 500 510 520 530 540 PPLPLHPRQQ QQQQQQQQQL QMQQQQQAQQ QQQQSIAGSI VGVDPASDMI SRFVKVVEPP 550 560 570 580 590 600 LWPNAQPCPM CMEELVHSAQ NPAISLSRCQ HLMHLQCLNG MIIAQQNEMN KNLFIECPVC 610 620 630 640 650 660 GIVYGEKVGN QPIGSMSWSI ISKNLPGHEG QNTIQIVYDI ASGLQTEEHP HPGRAFFAVG 670 680 690 700 710 720 FPRICYLPDC PLGRKVLRFL KIAFDRRLLF SIGRSVTTGR EDVVIWNSVD HKTQFNMFPD 730 PTYLQRTMQQ LVHLGVTD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A member of the Notch group of interacting loci, deltex encodes a cytoplasmic basic protein." Busseau I., Diederich R.J., Xu T., Artavanis-Tsakonas S. Genetics 136:585-596(1994) [PubMed: 8150285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: Oregon-R. Tissue: Embryo.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 17-738. Strain: Berkeley. Tissue: Embryo.
[6]	"Hitchhiking effects of recurrent beneficial amino acid substitutions in the Drosophila melanogaster genome." Andolfatto P. Genome Res. 17:1755-1762(2007) [PubMed: 17989248] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 326-567, VARIANTS LEU-511 AND GLN-514 INS. Strain: ZW104, ZW109, ZW122, ZW123, ZW133, ZW136, ZW139, ZW140, ZW141, ZW142, ZW143 and ZW144.
[7]	"Deltex acts as a positive regulator of Notch signaling through interactions with the Notch ankyrin repeats." Matsuno K., Diederich R.J., Go M.J., Blaumueller C.M., Artavanis-Tsakonas S. Development 121:2633-2644(1995) [PubMed: 7671825] [Abstract] Cited for: FUNCTION, INTERACTION WITH NOTCH.
[8]	"Novel Notch alleles reveal a Deltex-dependent pathway repressing neural fate." Ramain P., Khechumian K., Seugnet L., Arbogast N., Ackermann C., Heitzler P. Curr. Biol. 11:1729-1738(2001) [PubMed: 11719214] [Abstract] Cited for: FUNCTION.
[9]	"Involvement of a proline-rich motif and RING-H2 finger of Deltex in the regulation of Notch signaling." Matsuno K., Ito M., Hori K., Miyashita F., Suzuki S., Kishi N., Artavanis-Tsakonas S., Okano H. Development 129:1049-1059(2002) [PubMed: 11861487] [Abstract] Cited for: FUNCTION, HOMOMULTIMERIZATION, MUTAGENESIS OF 476-ARG--PRO-484; HIS-571 AND HIS-574.
[10]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-425, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U09789 mRNA. Translation: AAA18501.1.
AE014298 Genomic DNA. Translation: AAF46170.1.
BT033059 mRNA. Translation: ACE82582.1.
BT003476 mRNA. Translation: AAO39479.1. Different initiation.
EU217842 Genomic DNA. Translation: ABW92761.1.
EU217843 Genomic DNA. Translation: ABW92762.1.
EU217844 Genomic DNA. Translation: ABW92763.1.
EU217845 Genomic DNA. Translation: ABW92764.1.
EU217846 Genomic DNA. Translation: ABW92765.1.
EU217847 Genomic DNA. Translation: ABW92766.1.
EU217848 Genomic DNA. Translation: ABW92767.1.
EU217849 Genomic DNA. Translation: ABW92768.1.
EU217850 Genomic DNA. Translation: ABW92769.1.
EU217851 Genomic DNA. Translation: ABW92770.1.
EU217852 Genomic DNA. Translation: ABW92771.1.
EU217853 Genomic DNA. Translation: ABW92772.1.

PIR

S47857.

RefSeq

NP_511064.2. NM_078509.2.

UniGene

Dm.2626.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2A90	X-ray	2.15	A	26-261	[»]

ProteinModelPortal

Q23985.

SMR

Q23985. Positions 43-211, 545-604, 607-737.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-18159N.

IntAct

Q23985. 6 interactions.

MINT

MINT-333005.

STRING

Q23985.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblMetazoa

FBtr0070914; FBpp0070876; FBgn0000524.

GeneID

31589.

KEGG

dme:Dmel_CG3929.

NMPDR

fig|7227.3.peg.16733.

UCSC

CG3929-RA. d. melanogaster.

Organism-specific databases

CTD

31589.

FlyBase

FBgn0000524. dx.

Phylogenomic databases

eggNOG

inNOG06857.

GeneTree

EMGT00050000001525.

InParanoid

Q23985.

OMA

SIRRTQQ.

OrthoDB

EOG4Z8WBQ.

PhylomeDB

Q23985.

Gene expression databases

Bgee

Q23985.

GermOnline

CG3929. Drosophila melanogaster.

Family and domain databases

InterPro

IPR004170. WWE-dom.
IPR018123. WWE-dom_subgr.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]

Gene3D

G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.

KO

K06058.

Pfam

PF02825. WWE. 2 hits.
[Graphical view]

SMART

SM00184. RING. 1 hit.
SM00678. WWE. 2 hits.
[Graphical view]

PROSITE

PS50918. WWE. 2 hits.
PS00518. ZF_RING_1. False negative.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

774342.

Entry information

Entry name

DTX_DROME

Accession

Primary (citable) accession number: Q23985
Secondary accession number(s): A9YJA9 , A9YJB1, A9YJB7, B3LF71, Q86P53, Q9W3Z1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	August 15, 2003
Last modified:	January 25, 2012
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project