Q23983 (SNAP_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Soluble NSF attachment protein
Short name=SNAP

Alternative name(s):
N-ethylmaleimide-sensitive factor attachment protein

Gene names

Name:	Snap
ORF Names:	CG6625

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus. Also between the endosome and phagosome. Ref.5
Subcellular location	Cytoplasmic vesicle. Membrane; Peripheral membrane protein Ref.5.
Sequence similarities	Belongs to the SNAP family.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasmic vesicle Membrane
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	compound eye morphogenesis Inferred from mutant phenotype PubMed 15102912. Source: FlyBase cytokinesis Inferred from mutant phenotype PubMed 15380073. Source: FlyBase imaginal disc-derived wing expansion Inferred from mutant phenotype PubMed 15102912. Source: FlyBase intra-Golgi vesicle-mediated transport Inferred from mutant phenotype Ref.5. Source: UniProtKB intracellular protein transport Inferred from mutant phenotype Ref.5. Source: UniProtKB neuron projection morphogenesis Inferred from mutant phenotype PubMed 18604272. Source: FlyBase phagocytosis, engulfment Inferred from mutant phenotype PubMed 16336044. Source: FlyBase synaptic vesicle priming Non-traceable author statement PubMed 10798391. Source: FlyBase
Cellular_component	axon terminus Inferred from direct assay PubMed 15102912. Source: FlyBase cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmic vesicle Inferred from direct assay Ref.5. Source: UniProtKB membrane Inferred from direct assay Ref.5. Source: UniProtKB
Molecular_function	soluble NSF attachment protein activity Non-traceable author statement PubMed 10798391. Source: FlyBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Syx1A	Q24547	4	EBI-195132,EBI-135062

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Soluble NSF attachment protein		PRO_0000219065

Sequences

	Sequence		Length	Mass (Da)
	Q23983 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4EE50F038ACFEC5F	FASTA	292	33,000
	10 20 30 40 50 60 MGDNEQKALQ LMAEAEKKLT QQKGFLGSLF GGSNKVEDAI ECYQRAGNMF KMSKNWTKAG 70 80 90 100 110 120 ECFCEAATLH ARAGSRHDAG TCYVDASNCY KKVDVESAVN CLMKSIDIYT DMGRFTMAAK 130 140 150 160 170 180 HHQSIAEMYE SDPNNLAKSI QHYEQAADYF KGEESVSSAN KCMLKVAQYA AQLEDYEKAI 190 200 210 220 230 240 SIYEQVAASS LESSLLKYSA KEYFFRAALC HLSVDLLNAQ HAIEKYAQQY PAFQDSREFK 250 260 270 280 290 LIKVLCENLE EQNIEGFTEA VKDYDSISRL DQWYTTILLR IKKAADEDPD LR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Neurally expressed Drosophila genes encoding homologs of the NSF and SNAP secretory proteins." Ordway R.W., Pallanck L., Ganetzky B. Proc. Natl. Acad. Sci. U.S.A. 91:5715-5719(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Head.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"A systems biology analysis of the Drosophila phagosome." Stuart L.M., Boulais J., Charriere G.M., Hennessy E.J., Brunet S., Jutras I., Goyette G., Rondeau C., Letarte S., Huang H., Ye P., Morales F., Kocks C., Bader J.S., Desjardins M., Ezekowitz R.A.B. Nature 445:95-101(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U09374 mRNA. Translation: AAA83414.1. AE014296 Genomic DNA. Translation: AAF49035.1. AY069477 mRNA. Translation: AAL39622.1.
RefSeq	NP_524180.1. NM_079456.4.
UniGene	Dm.2107.
3D structure databases
ProteinModelPortal	Q23983.
SMR	Q23983. Positions 9-290.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-19393N.
IntAct	Q23983. 32 interactions.
MINT	MINT-325149.
STRING	7227.FBpp0074609.
Proteomic databases
PaxDb	Q23983.
PRIDE	Q23983.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0074840; FBpp0074609; FBgn0250791.
GeneID	40233.
KEGG	dme:Dmel_CG6625.
Organism-specific databases
CTD	40233.
FlyBase	FBgn0250791. Snap.
Phylogenomic databases
eggNOG	NOG245914.
GeneTree	ENSGT00390000005826.
InParanoid	Q23983.
KO	K15296.
OMA	SIAEMYE.
OrthoDB	EOG4D51DF.
PhylomeDB	Q23983.
Gene expression databases
Bgee	Q23983.
GermOnline	CG6625. Drosophila melanogaster.
Family and domain databases
Gene3D	1.25.40.10. 1 hit.
InterPro	IPR000744. NSF_attach. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. [Graphical view]
PANTHER	PTHR13768. PTHR13768. 1 hit.
Pfam	PF02071. NSF. 5 hits. PF13176. TPR_7. 1 hit. [Graphical view]
PRINTS	PR00448. NSFATTACHMNT.
ProtoNet	Search...
Other
GenomeRNAi	40233.
NextBio	817705.

Entry information

Entry name

SNAP_DROME

Accession

Primary (citable) accession number: Q23983
Secondary accession number(s): Q541G5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project