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Q23892

- GLUA_DICDI

UniProt

Q23892 - GLUA_DICDI

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Protein

Lysosomal beta glucosidase

Gene

gluA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei363 – 3631By similarity

GO - Molecular functioni

  1. beta-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH3. Glycoside Hydrolase Family 3.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal beta glucosidase (EC:3.2.1.21)
Gene namesi
Name:gluA
ORF Names:DDB_G0292810
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0292810. gluA.

Subcellular locationi

Lysosome 1 Publication

GO - Cellular componenti

  1. lysosome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Propeptidei25 – 69451 PublicationPRO_0000361523Add
BLAST
Chaini70 – 821752Lysosomal beta glucosidasePRO_0000361524Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi146 – 1461N-linked (GlcNAc...)Sequence Analysis
Glycosylationi266 – 2661N-linked (GlcNAc...)Sequence Analysis
Glycosylationi535 – 5351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi555 – 5551N-linked (GlcNAc...)Sequence Analysis
Glycosylationi703 – 7031N-linked (GlcNAc...)Sequence Analysis
Glycosylationi721 – 7211N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Glycosylated. The polyoligosaccharides are of the high-mannose type and are highly substituted with both phosphate and sulfate moieties.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ23892.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0215373.

Structurei

3D structure databases

ProteinModelPortaliQ23892.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi461 – 4666Poly-Ala
Compositional biasi571 – 5755Poly-Val

Sequence similaritiesi

Belongs to the glycosyl hydrolase 3 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1472.
InParanoidiQ23892.
KOiK05349.
OMAiITINETT.
PhylomeDBiQ23892.

Family and domain databases

Gene3Di3.20.20.300. 1 hit.
3.40.50.1700. 1 hit.
InterProiIPR026891. Fn3-like.
IPR026892. Glyco_hydro_3.
IPR002772. Glyco_hydro_3_C.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR30620. PTHR30620. 1 hit.
PfamiPF14310. Fn3-like. 1 hit.
PF00933. Glyco_hydro_3. 1 hit.
PF01915. Glyco_hydro_3_C. 1 hit.
[Graphical view]
PRINTSiPR00133. GLHYDRLASE3.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52279. SSF52279. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q23892 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKTIKSLFLL SLLIVNLLIS STYGSSIRVS IVGGEEAEVI EKPRTFGNKR
60 70 80 90 100
ELKLEYSQIY PKKQLNQENI NFMSARDTFV DNLMSKMSIT EKIGQMTQLD
110 120 130 140 150
ITTLTSPNTI TINETTLAYY AKTYYIGSYL NSPVSGGLAG DIHHINSSVW
160 170 180 190 200
LDMINTIQTI VIEGSPNKIP MIYGLDSVHG ANYVHKATLF PHNTGLAATF
210 220 230 240 250
NIEHATTAAQ ITSKDTVAVG IPWVFAPVLG IGVQPLWSRI YETFGEDPYV
260 270 280 290 300
ASMMGAAAVR GFQGGNNSFD GPINAPSAVC TAKHYFGYSD PTSGKDRTAA
310 320 330 340 350
WIPERMLRRY FLPSFAEAIT GAGAGTIMIN SGEVNGVPMH TSYKYLTEVL
360 370 380 390 400
RGELQFEGVA VTDWQDIEKL VYFHHTAGSA EEAILQALDA GIDMSMVPLD
410 420 430 440 450
LSFPIILAEM VAAGTVPESR LDLSVRRILN LKYALGLFSN PYPNPNAAIV
460 470 480 490 500
DTIGQVQDRE AAAATAEESI TLLQNKNNIL PLNTNTIKNV LLTGPSADSI
510 520 530 540 550
RNLNGGWSVH WQGAYEDSEF PFGTSILTGL REITNDTADF NIQYTIGHEI
560 570 580 590 600
GVPTNQTSID EAVELAQSSD VVVVVIGELP EAETPGDIYD LSMDPNEVLL
610 620 630 640 650
LQQLVDTGKP VVLILVEARP RILPPDLVYS CAAVLMAYLP GSEGGKPIAN
660 670 680 690 700
ILMGNVNPSG RLPLTYPGTT GDIGVPYYHK YSENGVTTPL FQFGDGLSYT
710 720 730 740 750
TFNYTNLACS NCKPISGQSG NYTGVLGQSY TFTVTVTNNG NVQGKDSVLL
760 770 780 790 800
YLSDLWAQVT PEVKMLRGFQ KVDLMPAKSQ QISFTLNAYE FSFIGVDNKI
810 820
TLESGQFIIM VGNQQLGLYL Q
Length:821
Mass (Da):89,344
Last modified:January 20, 2009 - v2
Checksum:i297758E5007DD13D
GO

Sequence cautioni

The sequence AAA74233.1 differs from that shown. Reason: Frameshift at positions 393, 397, 399, 402 and 409.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti290 – 2901D → N in AAA74233. (PubMed:8288612)Curated
Sequence conflicti474 – 4752QN → LF in AAA74233. (PubMed:8288612)Curated
Sequence conflicti725 – 7251Missing in AAA74233. (PubMed:8288612)Curated
Sequence conflicti737 – 7371T → TVT in AAA74233. (PubMed:8288612)Curated
Sequence conflicti806 – 8061Q → P in AAA74233. (PubMed:8288612)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L21014 mRNA. Translation: AAA74233.1. Frameshift.
AAFI02000197 Genomic DNA. Translation: EAL60954.1.
PIRiA49881.
RefSeqiXP_629427.1. XM_629425.1.

Genome annotation databases

EnsemblProtistsiDDB0215373; DDB0215373; DDB_G0292810.
GeneIDi8628946.
KEGGiddi:DDB_G0292810.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L21014 mRNA. Translation: AAA74233.1 . Frameshift.
AAFI02000197 Genomic DNA. Translation: EAL60954.1 .
PIRi A49881.
RefSeqi XP_629427.1. XM_629425.1.

3D structure databases

ProteinModelPortali Q23892.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0215373.

Protein family/group databases

CAZyi GH3. Glycoside Hydrolase Family 3.

Proteomic databases

PRIDEi Q23892.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0215373 ; DDB0215373 ; DDB_G0292810 .
GeneIDi 8628946.
KEGGi ddi:DDB_G0292810.

Organism-specific databases

dictyBasei DDB_G0292810. gluA.

Phylogenomic databases

eggNOGi COG1472.
InParanoidi Q23892.
KOi K05349.
OMAi ITINETT.
PhylomeDBi Q23892.

Miscellaneous databases

PROi Q23892.

Family and domain databases

Gene3Di 3.20.20.300. 1 hit.
3.40.50.1700. 1 hit.
InterProi IPR026891. Fn3-like.
IPR026892. Glyco_hydro_3.
IPR002772. Glyco_hydro_3_C.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR30620. PTHR30620. 1 hit.
Pfami PF14310. Fn3-like. 1 hit.
PF00933. Glyco_hydro_3. 1 hit.
PF01915. Glyco_hydro_3_C. 1 hit.
[Graphical view ]
PRINTSi PR00133. GLHYDRLASE3.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF52279. SSF52279. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of the full-length cDNA encoding the developmentally regulated lysosomal enzyme beta-glucosidase in Dictyostelium discoideum."
    Bush J., Richardson J., Cardelli J.
    J. Biol. Chem. 269:1468-1476(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 70-78; 74-82; 204-213 AND 583-592, DEVELOPMENTAL STAGE.
    Strain: AX3.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "Structural analysis of the asparagine-linked oligosaccharides from three lysosomal enzymes of Dictyostelium discoideum. Evidence for an unusual acid-stable phosphodiester."
    Freeze H.H., Yeh R., Miller A.L., Kornfeld S.
    J. Biol. Chem. 258:14874-14879(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION.
  4. "Lysosomal enzymes in Dictyostelium discoideum are transported to lysosomes at distinctly different rates."
    Cardelli J.A., Golumbeski G.S., Dimond R.L.
    J. Cell Biol. 102:1264-1270(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiGLUA_DICDI
AccessioniPrimary (citable) accession number: Q23892
Secondary accession number(s): Q54CI9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: January 20, 2009
Last modified: October 29, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Caution

PubMed:8288612 reports 2 different N-termini by direct protein sequencing: mature protein either starts at Ile-70 or Ser-74.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3