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Protein

NADH-ubiquinone oxidoreductase 49 kDa subunit

Gene

nad7

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). Component of the iron-sulfur (IP) fragment of the enzyme. Component of the iron-sulfur (IP) fragment of the enzyme (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
  3. quinone binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 49 kDa subunit (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
NADH dehydrogenase subunit 7
Gene namesi
Name:nad7
Synonyms:ndufs2
ORF Names:DDB_G0294030
Encoded oniMitochondrion
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Mitochondrion

Organism-specific databases

dictyBaseiDDB_G0294030. nad7.

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
  2. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 406406NADH-ubiquinone oxidoreductase 49 kDa subunitPRO_0000118589Add
BLAST

Interactioni

Subunit structurei

Complex I is composed of about 45 different subunits (By similarity). Interacts with NDUFAF7.By similarity1 Publication

Protein-protein interaction databases

STRINGi44689.DidioMp18.

Structurei

3D structure databases

ProteinModelPortaliQ23883.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 49 kDa subunit family.Curated

Phylogenomic databases

eggNOGiCOG0649.
InParanoidiQ23883.
KOiK03935.
OMAiEYRTWTQ.
PhylomeDBiQ23883.

Family and domain databases

Gene3Di1.10.645.10. 2 hits.
HAMAPiMF_01358. NDH1_NuoD.
InterProiIPR001135. NADH_Q_OxRdtase_suD.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR022885. NDH1_su_D/H.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00346. Complex1_49kDa. 1 hit.
[Graphical view]
SUPFAMiSSF56762. SSF56762. 1 hit.
TIGRFAMsiTIGR01962. NuoD. 1 hit.
PROSITEiPS00535. COMPLEX1_49K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q23883-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNISKIFEE VKVMKNFTLN FGPQHPAAHG VLRLIVELES ENVVRVEPHI
60 70 80 90 100
GLLHRGTEKL IEGKTYTQAL PYFDRLDYVS MNVQEHAYSL AVERLYLDSL
110 120 130 140 150
DIELEIPQRA KVIRVLFSEI TRVLNHIMAT TTHAMDVGAL TPFLWAFEER
160 170 180 190 200
EKLMEFYERV SGARMHAAYI RPGGVAFDLP MNISEDIYKF VIQYRKRLEE
210 220 230 240 250
IEDMLINNRI WKQRLVDIGI VSAEEALNYG FTGPLLRGAG IVYDIRKNYP
260 270 280 290 300
YDDYDKYDFK IIIGEENNSY TRFIIRMKEM YQSLSIIEQA LNNLRPGLIK
310 320 330 340 350
LEGVNITAPD RAFVKKDMES CINHFKFFSE GFIIPANENY TIVEAPKGEF
360 370 380 390 400
GIYLNANDTA KPYRCRIKAP GFLHLQGLNM MSKDHLLADV VTLIGTQDIV

FGEVDR
Length:406
Mass (Da):46,833
Last modified:November 1, 1996 - v1
Checksum:i581BCF7AD71F86A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21196 Genomic DNA. Translation: BAA04731.1.
AB000109 Genomic DNA. Translation: BAA78067.1.
PIRiT43763.
RefSeqiNP_050085.1. NC_000895.1.

Genome annotation databases

GeneIDi2193913.
KEGGiddi:DidioMp18.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21196 Genomic DNA. Translation: BAA04731.1.
AB000109 Genomic DNA. Translation: BAA78067.1.
PIRiT43763.
RefSeqiNP_050085.1. NC_000895.1.

3D structure databases

ProteinModelPortaliQ23883.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DidioMp18.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2193913.
KEGGiddi:DidioMp18.

Organism-specific databases

dictyBaseiDDB_G0294030. nad7.

Phylogenomic databases

eggNOGiCOG0649.
InParanoidiQ23883.
KOiK03935.
OMAiEYRTWTQ.
PhylomeDBiQ23883.

Miscellaneous databases

PROiQ23883.

Family and domain databases

Gene3Di1.10.645.10. 2 hits.
HAMAPiMF_01358. NDH1_NuoD.
InterProiIPR001135. NADH_Q_OxRdtase_suD.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR022885. NDH1_su_D/H.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00346. Complex1_49kDa. 1 hit.
[Graphical view]
SUPFAMiSSF56762. SSF56762. 1 hit.
TIGRFAMsiTIGR01962. NuoD. 1 hit.
PROSITEiPS00535. COMPLEX1_49K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mitochondrial ribosomal protein L11 gene of Dictyostelium discoideum resides not in the nuclear genome but in the mitochondrial genome."
    Iwamoto M., Yanagisawa K., Tanaka Y.
    DNA Res. 2:129-132(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: AX3.
  2. "The mitochondrial DNA of Dictyostelium discoideum: complete sequence, gene content and genome organization."
    Ogawa S., Yoshino R., Angata K., Iwamoto M., Pi M., Kuroe K., Matsuo K., Morio T., Urushihara H., Yanagisawa K., Tanaka Y.
    Mol. Gen. Genet. 263:514-519(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX3.
  3. Bienvenut W.V., Ura S., Insall R.H.
    Submitted (JUL-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 34-45; 115-122; 238-246 AND 261-272, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: AX2.
  4. Cited for: INTERACTION WITH NDUFAF7.

Entry informationi

Entry nameiNDUS2_DICDI
AccessioniPrimary (citable) accession number: Q23883
Secondary accession number(s): Q9XPJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.