Q23883 (NDUS2_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-ubiquinone oxidoreductase 49 kDa subunit
EC=1.6.5.3
EC=1.6.99.3

Alternative name(s):
NADH dehydrogenase subunit 7

Gene names

Name:	nad7
Synonyms:	ndufs2
ORF Names:	DDB_G0294030

Encoded on

Mitochondrion

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. Component of the iron-sulfur (IP) fragment of the enzyme. Component of the iron-sulfur (IP) fragment of the enzyme By similarity.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol. NADH + acceptor = NAD+ + reduced acceptor.
Subunit structure	Complex I is composed of about 45 different subunits By similarity. Interacts with NDUFAF7. Ref.4
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	NADH-ubiquinone oxidoreductase 49 kDa subunit		PRO_0000118589

Sequences

	Sequence		Length	Mass (Da)
	Q23883 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 581BCF7AD71F86A8	FASTA	406	46,833
	10 20 30 40 50 60 MLNISKIFEE VKVMKNFTLN FGPQHPAAHG VLRLIVELES ENVVRVEPHI GLLHRGTEKL 70 80 90 100 110 120 IEGKTYTQAL PYFDRLDYVS MNVQEHAYSL AVERLYLDSL DIELEIPQRA KVIRVLFSEI 130 140 150 160 170 180 TRVLNHIMAT TTHAMDVGAL TPFLWAFEER EKLMEFYERV SGARMHAAYI RPGGVAFDLP 190 200 210 220 230 240 MNISEDIYKF VIQYRKRLEE IEDMLINNRI WKQRLVDIGI VSAEEALNYG FTGPLLRGAG 250 260 270 280 290 300 IVYDIRKNYP YDDYDKYDFK IIIGEENNSY TRFIIRMKEM YQSLSIIEQA LNNLRPGLIK 310 320 330 340 350 360 LEGVNITAPD RAFVKKDMES CINHFKFFSE GFIIPANENY TIVEAPKGEF GIYLNANDTA 370 380 390 400 KPYRCRIKAP GFLHLQGLNM MSKDHLLADV VTLIGTQDIV FGEVDR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mitochondrial ribosomal protein L11 gene of Dictyostelium discoideum resides not in the nuclear genome but in the mitochondrial genome." Iwamoto M., Yanagisawa K., Tanaka Y. DNA Res. 2:129-132(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AX3.
[2]	"The mitochondrial DNA of Dictyostelium discoideum: complete sequence, gene content and genome organization." Ogawa S., Yoshino R., Angata K., Iwamoto M., Pi M., Kuroe K., Matsuo K., Morio T., Urushihara H., Yanagisawa K., Tanaka Y. Mol. Gen. Genet. 263:514-519(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX3.
[3]	Bienvenut W.V., Ura S., Insall R.H. Submitted (JUL-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 34-45; 115-122; 238-246 AND 261-272, MASS SPECTROMETRY. Strain: AX2.
[4]	"MidA is a putative methyltransferase that is required for mitochondrial complex I function." Carilla-Latorre S., Gallardo M.E., Annesley S.J., Calvo-Garrido J., Grana O., Accari S.L., Smith P.K., Valencia A., Garesse R., Fisher P.R., Escalante R. J. Cell Sci. 123:1674-1683(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NDUFAF7.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D21196 Genomic DNA. Translation: BAA04731.1. AB000109 Genomic DNA. Translation: BAA78067.1.
PIR	T43763.
RefSeq	NP_050085.1. NC_000895.1.
3D structure databases
ProteinModelPortal	Q23883.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DidioMp18.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2193913.
KEGG	ddi:DidioMp18.
Organism-specific databases
dictyBase	DDB_G0294030. nad7.
Phylogenomic databases
eggNOG	COG0649.
KO	K03935.
OMA	QSNRIIK.
Family and domain databases
InterPro	IPR001135. NADH_Q_OxRdtase_suD. IPR014029. NADH_UbQ_OxRdtase_49kDa_CS. IPR022885. NDH1_su_D/H. [Graphical view]
Pfam	PF00346. Complex1_49kDa. 1 hit. [Graphical view]
TIGRFAMs	TIGR01962. NuoD. 1 hit.
PROSITE	PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS2_DICDI

Accession

Primary (citable) accession number: Q23883
Secondary accession number(s): Q9XPJ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)