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Protein

Serine protease/ABC transporter B family protein tagC

Gene

tagC

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May be directly involved in maturation and export of SDF-2. The exposure of the protease domain of tagC on the surface of the prestalk cells were it can convert acbA to SDF-2 is induced by GABA. Intercellular communication via tagC may mediate integration of cellular differentiation with morphogenesis.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei325Charge relay systemPROSITE-ProRule annotation1
Active sitei372Charge relay systemPROSITE-ProRule annotation1
Active sitei637Charge relay systemPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi1485 – 1492ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity, coupled to transmembrane movement of substances Source: dictyBase
  • ATP binding Source: UniProtKB-KW
  • serine-type endopeptidase activity Source: InterPro
  • serine-type peptidase activity Source: dictyBase

GO - Biological processi

  • cell differentiation Source: dictyBase
  • proteolysis Source: dictyBase
  • transport Source: dictyBase

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processTransport
LigandATP-binding, Nucleotide-binding

Protein family/group databases

MEROPSiS08.127.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease/ABC transporter B family protein tagC (EC:3.4.21.-)
Alternative name(s):
Prestalk-specific protein tagC
Gene namesi
Name:tagC
ORF Names:DDB_G0286121
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0286121. tagC.

Subcellular locationi

Q23868:
  • Membrane PROSITE-ProRule annotation; Multi-pass membrane protein PROSITE-ProRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei962 – 982HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei1027 – 1047HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei1072 – 1092HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei1157 – 1177HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei1260 – 1280HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei1288 – 1308HelicalPROSITE-ProRule annotationAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: dictyBase

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Cells in which tagC is disrupted do not form SDF-2. Null mutations in either tagB or tagC lead to a cell autonomous defect in prestalk differentiation and a 5-fold reduction in the expression of the prestalk gene ecmA. Disruption of regA in a tagB null or in a tagC null background results in higher levels of sporulation.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000002719228 – 1741Serine protease/ABC transporter B family protein tagCAdd BLAST1714

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi390N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi536N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi547N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi614N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi689N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi735N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi741N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi776N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi832N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi887N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1251N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1385N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1386N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1454N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1704N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ23868.

Expressioni

Developmental stagei

Found exclusively in prestalk cells.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0191192.

Structurei

3D structure databases

ProteinModelPortaliQ23868.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini298 – 698Peptidase S8Add BLAST401
Domaini1031 – 1314ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST284
Domaini1450 – 1687ABC transporterPROSITE-ProRule annotationAdd BLAST238

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi42 – 46Poly-Asn5
Compositional biasi94 – 103Poly-Asn10
Compositional biasi643 – 646Poly-Ala4
Compositional biasi733 – 741Poly-Asn9
Compositional biasi786 – 792Poly-Ser7
Compositional biasi1337 – 1340Poly-Glu4
Compositional biasi1346 – 1352Poly-Gly7
Compositional biasi1353 – 1357Poly-Asn5
Compositional biasi1358 – 1364Poly-Asp7
Compositional biasi1381 – 1386Poly-Asn6
Compositional biasi1707 – 1729Poly-AsnAdd BLAST23

Sequence similaritiesi

In the N-terminal section; belongs to the peptidase S8 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0055. Eukaryota.
KOG0058. Eukaryota.
COG1132. LUCA.
InParanoidiQ23868.
OMAiYIIMIVA.
PhylomeDBiQ23868.

Family and domain databases

CDDicd04842. Peptidases_S8_Kp43_protease. 1 hit.
Gene3Di1.20.1560.10. 1 hit.
3.40.50.200. 1 hit.
InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR036640. ABC1_TM_sf.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR008979. Galactose-bd-like.
IPR027417. P-loop_NTPase.
IPR000209. Peptidase_S8/S53_dom.
IPR036852. Peptidase_S8/S53_dom_sf.
IPR022398. Peptidase_S8_His-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR034058. TagA/B/C/D_pept_dom.
PfamiView protein in Pfam
PF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
PF00082. Peptidase_S8. 1 hit.
PRINTSiPR00723. SUBTILISIN.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF52743. SSF52743. 3 hits.
SSF90123. SSF90123. 1 hit.
PROSITEiView protein in PROSITE
PS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q23868-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFHFSSNKL LLISGLILLV LVIGIKLDIF LSSKSTEYIK FNNNNNHFKR
60 70 80 90 100
DKRILLHNEI IDTNNKPSIK NNQIFINDND NNENISPILL RILNNNNNNN
110 120 130 140 150
NNNGDDKSIY KKNHYIVQFK DRINDETREQ LKEFLIGTDI VLDEQPYQSH
160 170 180 190 200
IVHYIPHDSF LVLMTQEQSV LLSSKEWVSW IGEFEPSNKI HLNYNEKSIG
210 220 230 240 250
LPVYIILSDS TNSLIQRWEN TLNSILKSYN SKVKLTLINQ KKLKSIVYCN
260 270 280 290 300
DESPSPSCSL INSEKLVYQW ISEQSESNFI ERSEKFQTAN RLSPKVVFGT
310 320 330 340 350
KDTLVNNDRV DIPLRGKGQI LSIADTGLDG SHCFFSDSKY PIPLNSVNLN
360 370 380 390 400
HRKVVTYITT STSDDSDKVD GHGTHICGSA AGTPEDSSVN ISSFSGLATD
410 420 430 440 450
AKIAFFDLAS GSSSLTPPSD LKQLYQPLYD AGARVHCDSW GSVSVEGYTG
460 470 480 490 500
SYSSDTASID DFLFTHPDFI ILRAAGNNEQ YLSLLTQSTA KNVITVGAHQ
510 520 530 540 550
TIHENYLTDG PNYINYQSSV DINQELICDF DSRYCNYTTA QCCLESNATT
560 570 580 590 600
GLASCCPTLL RKSVIDAANT QPLLYNENNI CSFSSKGPTH DGRMKPDLVA
610 620 630 640 650
PGEYITSARS NGANTTDQCG DGSLPNTNAL LAISGTSMAT SFAAAATTIL
660 670 680 690 700
RQYLVDGYYP TGSIVESNKL QPTGSLLKAL MINNAQLLNG TFQLITSSSI
710 720 730 740 750
TYPSNQVFEN FAGASLVQGW GAIRMSNWLH VVNNNNSNNN NKTSDGITKF
760 770 780 790 800
VGIGGLDLRL VKPNQWKEES LSTGQNTSYC FTYKPSSSSS NSGNNIPRVV
810 820 830 840 850
ATLVWTDPPS YAGAKFNLVN NLDLTMIYYR DNGSTIFYSN QGGSSFLGLA
860 870 880 890 900
PTQDTLNNVE GIVHNPTEPM TYRFMVAGTN VPMGPQNFSF VFHGENGEFE
910 920 930 940 950
WADKCPQCSP GQVLACPVEN GIGTQLCTSD LQWSSCLIQS CDNNYNYNSI
960 970 980 990 1000
KKRCDKFLSY NYIIMIVAGG TMLLIIFILL LIKYQEYKES KKDSFRRFDD
1010 1020 1030 1040 1050
GTGIFVRPKD KDAKVSLADL YNLISPFIIE LAISTACSLV STAASILQPF
1060 1070 1080 1090 1100
YIGQIINDIP TAKHIGDFKS QFILIFILAV IEFVFSTISS WISGIVNEKM
1110 1120 1130 1140 1150
VMRLQNKVFR ALIAQDMGFF QKNNAAILMN VLISDTPMLR SALTGILLSI
1160 1170 1180 1190 1200
ATGLCKFVGS LVFIFTISWK LSLVFFATVP VLAFITQIQS QFTKRLTRAL
1210 1220 1230 1240 1250
LFYNSKASQH GQESMVNMHV VTNYCRQDKE IIKYTDNLNN VFQTARRLII
1260 1270 1280 1290 1300
NNTLASSIKW LMVESLAFII LYYGAYLAIQ KQFTVGLLIS FCLYIGYVID
1310 1320 1330 1340 1350
STTTLFGVYV SYVQSQASAT RVFMILRSAP RKRTTLEEEE ADRNNGLGGG
1360 1370 1380 1390 1400
GGNNNNNDDD DDDDNYNGKG NDNGKGSDDP NNNNNNTTLE NIDDNGELTT
1410 1420 1430 1440 1450
VQPVELTKKQ LKKQKEKEQK EYFKRTGISV VEMSLIPSSY IELTDCKGEI
1460 1470 1480 1490 1500
EFKNVSFRYP TRPDVQVLHN INMKFETGKC YGLVGPSGSG KSTLLELISK
1510 1520 1530 1540 1550
FYPLRDGGNI IMDDIDIANI RPNNLRGFVT CVHQNPYLFD ASIKDNIGYA
1560 1570 1580 1590 1600
LDNPTIEEVI EAAKLAYAHE FIKDLPQQYD TVLGSAGSLL SGGQKKRIAI
1610 1620 1630 1640 1650
ARAICAKRKI MLLDEITAEL DPESEEAITQ SIKVLTQGHT VVMVAHKVAA
1660 1670 1680 1690 1700
VRDCDKIFVL DKGYLVEEGT HDELMERKGK YHRMFNNEKD EEELLNNLGL
1710 1720 1730 1740
PSNNETNNEN NNENNNNNNN NNNNNNNNND DNNNQVVGEQ V
Length:1,741
Mass (Da):193,884
Last modified:December 4, 2007 - v2
Checksum:i44DEC61D68E4947D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti597D → A in AAB03331 (PubMed:8986798).Curated1
Sequence conflicti751V → D in AAB03331 (PubMed:8986798).Curated1
Sequence conflicti1740 – 1741QV → KFKN in AAB03331 (PubMed:8986798).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60086 mRNA. Translation: AAB03331.1.
AAFI02000085 Genomic DNA. Translation: EAL64353.1.
PIRiT18279.
RefSeqiXP_637864.1. XM_632772.1.

Genome annotation databases

EnsemblProtistsiEAL64353; EAL64353; DDB_G0286121.
GeneIDi8625461.
KEGGiddi:DDB_G0286121.

Similar proteinsi

Entry informationi

Entry nameiTAGC_DICDI
AccessioniPrimary (citable) accession number: Q23868
Secondary accession number(s): Q54M84
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 4, 2007
Last modified: October 25, 2017
This is version 115 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families