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Protein

Calreticulin

Gene

crtA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei109 – 1091CarbohydrateBy similarity
Binding sitei111 – 1111CarbohydrateBy similarity
Binding sitei128 – 1281CarbohydrateBy similarity
Binding sitei135 – 1351CarbohydrateBy similarity
Binding sitei318 – 3181CarbohydrateBy similarity

GO - Molecular functioni

  • calcium ion binding Source: dictyBase
  • carbohydrate binding Source: UniProtKB-KW

GO - Biological processi

  • phagocytosis, engulfment Source: dictyBase
  • protein folding Source: InterPro
  • response to other organism Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

Calcium, Lectin, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_305163. Calnexin/calreticulin cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Calreticulin
Gene namesi
Name:crtA
ORF Names:DDB_G0283539
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0283539. crtA.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: dictyBase
  • endoplasmic reticulum lumen Source: dictyBase
  • phagocytic vesicle Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 424405CalreticulinPRO_0000004181Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi105 ↔ 137By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ23858.

Interactioni

Protein-protein interaction databases

IntActiQ23858. 1 interaction.
STRINGi44689.DDB0191384.

Structurei

3D structure databases

ProteinModelPortaliQ23858.
SMRiQ23858. Positions 205-303.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati191 – 202121-1Add
BLAST
Repeati210 – 221121-2Add
BLAST
Repeati227 – 238121-3Add
BLAST
Repeati246 – 256111-4Add
BLAST
Repeati260 – 270112-1Add
BLAST
Repeati274 – 284112-2Add
BLAST
Repeati288 – 298112-3Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni191 – 256664 X 12 AA approximate repeatsAdd
BLAST
Regioni260 – 298393 X 11 AA approximate repeatsAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi421 – 4244Prevents secretion from ERPROSITE-ProRule annotation

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi353 – 42068Asp/Glu/Lys-richAdd
BLAST

Domaini

Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity (By similarity).By similarity
The interaction with glycans occurs through a binding site in the globular lectin domain.By similarity
The zinc binding sites are localized to the N-domain.By similarity

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG305105.
InParanoidiQ23858.
KOiK08057.
OMAiMEDDWDF.
PhylomeDBiQ23858.

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009169. Calreticulin.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PIRSFiPIRSF002356. Calreticulin. 1 hit.
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q23858-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLLLCLIFL VFVFNFALST VHFKDTFDND WESRWVVSDW HKEDGKSGKL
60 70 80 90 100
VHTAGKWFGD ENQKGIQTSE DARFYAVSAK FPSFSNKGKD LVLQYTVKNE
110 120 130 140 150
QKVDCGGSYI KLLPSKLDQS AFDGESEYSI MFGPDVCGAS KRVHVILNYK
160 170 180 190 200
GKNHLIKKEI NKVETDQLTH QYTLVISPDN TYKVLVDNKE IQAGNLADDW
210 220 230 240 250
ELLPSKQIKD PKQSKPVDWV DVKEIDDPED VKPAGHDDIP ASIVDPEAVK
260 270 280 290 300
PEDWNEEDDG EWEAPTIANP EYKGEWKAKK IPNPEYKGEW VHPLIDNPEY
310 320 330 340 350
AEDNELYLFN DLGAIGFELW QVKSGSIFNN MIVTDSVEEA KDFSEKTFVA
360 370 380 390 400
NQEAEKKMFD DLEAAKAEER KKADEKLAAE KAAEKEAEEA DEEEEEVAEE
410 420
DLVKTDDKKE EVKKSTKKVD HDEL
Length:424
Mass (Da):48,365
Last modified:December 4, 2007 - v3
Checksum:iEAF3D804F96E4AEC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti183 – 1831K → N in AAB87719 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36937 mRNA. Translation: AAB87719.1.
AAFI02000055 Genomic DNA. Translation: EAL65647.1.
RefSeqiXP_639010.1. XM_633918.1.

Genome annotation databases

EnsemblProtistsiDDB0191384; DDB0191384; DDB_G0283539.
GeneIDi8624140.
KEGGiddi:DDB_G0283539.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36937 mRNA. Translation: AAB87719.1.
AAFI02000055 Genomic DNA. Translation: EAL65647.1.
RefSeqiXP_639010.1. XM_633918.1.

3D structure databases

ProteinModelPortaliQ23858.
SMRiQ23858. Positions 205-303.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ23858. 1 interaction.
STRINGi44689.DDB0191384.

Proteomic databases

PRIDEiQ23858.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0191384; DDB0191384; DDB_G0283539.
GeneIDi8624140.
KEGGiddi:DDB_G0283539.

Organism-specific databases

dictyBaseiDDB_G0283539. crtA.

Phylogenomic databases

eggNOGiNOG305105.
InParanoidiQ23858.
KOiK08057.
OMAiMEDDWDF.
PhylomeDBiQ23858.

Enzyme and pathway databases

ReactomeiREACT_305163. Calnexin/calreticulin cycle.

Miscellaneous databases

PROiQ23858.

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009169. Calreticulin.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PIRSFiPIRSF002356. Calreticulin. 1 hit.
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Mueller-Taubenberger A., Gerisch G.
    Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: AX3.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "Identification of novel centrosomal proteins in Dictyostelium discoideum by comparative proteomic approaches."
    Reinders Y., Schulz I., Graef R., Sickmann A.
    J. Proteome Res. 5:589-598(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  4. "Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
    Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
    Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: AX2.

Entry informationi

Entry nameiCALR_DICDI
AccessioniPrimary (citable) accession number: Q23858
Secondary accession number(s): Q54QX4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 4, 2007
Last modified: July 22, 2015
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.