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Q23858 (CALR_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calreticulin
Gene names
Name:crtA
ORF Names:DDB_G0283539
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length424 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER By similarity.

Subcellular location

Endoplasmic reticulum lumen.

Domain

Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity By similarity.

The interaction with glycans occurs through a binding site in the globular lectin domain By similarity.

The zinc binding sites are localized to the N-domain By similarity.

Sequence similarities

Belongs to the calreticulin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 424405Calreticulin
PRO_0000004181

Regions

Repeat191 – 202121-1
Repeat210 – 221121-2
Repeat227 – 238121-3
Repeat246 – 256111-4
Repeat260 – 270112-1
Repeat274 – 284112-2
Repeat288 – 298112-3
Region191 – 256664 X 12 AA approximate repeats
Region260 – 298393 X 11 AA approximate repeats
Motif421 – 4244Prevents secretion from ER Potential
Compositional bias353 – 42068Asp/Glu/Lys-rich

Sites

Binding site1091Carbohydrate By similarity
Binding site1111Carbohydrate By similarity
Binding site1281Carbohydrate By similarity
Binding site1351Carbohydrate By similarity
Binding site3181Carbohydrate By similarity

Amino acid modifications

Disulfide bond105 ↔ 137 By similarity

Experimental info

Sequence conflict1831K → N in AAB87719. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q23858 [UniParc].

Last modified December 4, 2007. Version 3.
Checksum: EAF3D804F96E4AEC

FASTA42448,365
        10         20         30         40         50         60 
MRLLLCLIFL VFVFNFALST VHFKDTFDND WESRWVVSDW HKEDGKSGKL VHTAGKWFGD 

        70         80         90        100        110        120 
ENQKGIQTSE DARFYAVSAK FPSFSNKGKD LVLQYTVKNE QKVDCGGSYI KLLPSKLDQS 

       130        140        150        160        170        180 
AFDGESEYSI MFGPDVCGAS KRVHVILNYK GKNHLIKKEI NKVETDQLTH QYTLVISPDN 

       190        200        210        220        230        240 
TYKVLVDNKE IQAGNLADDW ELLPSKQIKD PKQSKPVDWV DVKEIDDPED VKPAGHDDIP 

       250        260        270        280        290        300 
ASIVDPEAVK PEDWNEEDDG EWEAPTIANP EYKGEWKAKK IPNPEYKGEW VHPLIDNPEY 

       310        320        330        340        350        360 
AEDNELYLFN DLGAIGFELW QVKSGSIFNN MIVTDSVEEA KDFSEKTFVA NQEAEKKMFD 

       370        380        390        400        410        420 
DLEAAKAEER KKADEKLAAE KAAEKEAEEA DEEEEEVAEE DLVKTDDKKE EVKKSTKKVD 


HDEL 

« Hide

References

« Hide 'large scale' references
[1]Mueller-Taubenberger A., Gerisch G.
Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: AX3.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"Identification of novel centrosomal proteins in Dictyostelium discoideum by comparative proteomic approaches."
Reinders Y., Schulz I., Graef R., Sickmann A.
J. Proteome Res. 5:589-598(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[4]"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: AX2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U36937 mRNA. Translation: AAB87719.1.
AAFI02000055 Genomic DNA. Translation: EAL65647.1.
RefSeqXP_639010.1. XM_633918.1.

3D structure databases

ProteinModelPortalQ23858.
SMRQ23858. Positions 205-303.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ23858. 1 interaction.
STRING44689.DDB_0191384.

Proteomic databases

PRIDEQ23858.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0191384; DDB0191384; DDB_G0283539.
GeneID8624140.
KEGGddi:DDB_G0283539.

Organism-specific databases

dictyBaseDDB_G0283539. crtA.

Phylogenomic databases

eggNOGNOG305105.
OMASEYSIMF.
PhylomeDBQ23858.

Family and domain databases

Gene3D2.10.250.10. 1 hit.
2.60.120.200. 1 hit.
InterProIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009169. Calreticulin.
IPR009033. Calreticulin/calnexin_P_dom.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
[Graphical view]
PANTHERPTHR11073. PTHR11073. 1 hit.
PfamPF00262. Calreticulin. 1 hit.
[Graphical view]
PIRSFPIRSF002356. Calreticulin. 1 hit.
PRINTSPR00626. CALRETICULIN.
SUPFAMSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEPS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ23858.

Entry information

Entry nameCALR_DICDI
AccessionPrimary (citable) accession number: Q23858
Secondary accession number(s): Q54QX4
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 4, 2007
Last modified: July 9, 2014
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase