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Protein

Calreticulin

Gene

crtA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei109CarbohydrateBy similarity1
Binding sitei111CarbohydrateBy similarity1
Binding sitei128CarbohydrateBy similarity1
Binding sitei135CarbohydrateBy similarity1
Binding sitei318CarbohydrateBy similarity1

GO - Molecular functioni

  • calcium ion binding Source: dictyBase
  • carbohydrate binding Source: UniProtKB-KW

GO - Biological processi

  • phagocytosis, engulfment Source: dictyBase
  • protein folding Source: InterPro
  • response to other organism Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

Calcium, Lectin, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DDI-901042. Calnexin/calreticulin cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Calreticulin
Gene namesi
Name:crtA
ORF Names:DDB_G0283539
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0283539. crtA.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: dictyBase
  • endoplasmic reticulum lumen Source: dictyBase
  • phagocytic vesicle Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000000418120 – 424CalreticulinAdd BLAST405

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi105 ↔ 137By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ23858.

Interactioni

Protein-protein interaction databases

IntActiQ23858. 1 interactor.
STRINGi44689.DDB0191384.

Structurei

3D structure databases

ProteinModelPortaliQ23858.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati191 – 2021-1Add BLAST12
Repeati210 – 2211-2Add BLAST12
Repeati227 – 2381-3Add BLAST12
Repeati246 – 2561-4Add BLAST11
Repeati260 – 2702-1Add BLAST11
Repeati274 – 2842-2Add BLAST11
Repeati288 – 2982-3Add BLAST11

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni191 – 2564 X 12 AA approximate repeatsAdd BLAST66
Regioni260 – 2983 X 11 AA approximate repeatsAdd BLAST39

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi421 – 424Prevents secretion from ERPROSITE-ProRule annotation4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi353 – 420Asp/Glu/Lys-richAdd BLAST68

Domaini

Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity (By similarity).By similarity
The interaction with glycans occurs through a binding site in the globular lectin domain.By similarity
The zinc binding sites are localized to the N-domain.By similarity

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG0674. Eukaryota.
ENOG410XRR7. LUCA.
InParanoidiQ23858.
KOiK08057.
OMAiDICGSST.
PhylomeDBiQ23858.

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009169. Calreticulin.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 2 hits.
[Graphical view]
PIRSFiPIRSF002356. Calreticulin. 1 hit.
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q23858-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLLLCLIFL VFVFNFALST VHFKDTFDND WESRWVVSDW HKEDGKSGKL
60 70 80 90 100
VHTAGKWFGD ENQKGIQTSE DARFYAVSAK FPSFSNKGKD LVLQYTVKNE
110 120 130 140 150
QKVDCGGSYI KLLPSKLDQS AFDGESEYSI MFGPDVCGAS KRVHVILNYK
160 170 180 190 200
GKNHLIKKEI NKVETDQLTH QYTLVISPDN TYKVLVDNKE IQAGNLADDW
210 220 230 240 250
ELLPSKQIKD PKQSKPVDWV DVKEIDDPED VKPAGHDDIP ASIVDPEAVK
260 270 280 290 300
PEDWNEEDDG EWEAPTIANP EYKGEWKAKK IPNPEYKGEW VHPLIDNPEY
310 320 330 340 350
AEDNELYLFN DLGAIGFELW QVKSGSIFNN MIVTDSVEEA KDFSEKTFVA
360 370 380 390 400
NQEAEKKMFD DLEAAKAEER KKADEKLAAE KAAEKEAEEA DEEEEEVAEE
410 420
DLVKTDDKKE EVKKSTKKVD HDEL
Length:424
Mass (Da):48,365
Last modified:December 4, 2007 - v3
Checksum:iEAF3D804F96E4AEC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti183K → N in AAB87719 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36937 mRNA. Translation: AAB87719.1.
AAFI02000055 Genomic DNA. Translation: EAL65647.1.
RefSeqiXP_639010.1. XM_633918.1.

Genome annotation databases

EnsemblProtistsiEAL65647; EAL65647; DDB_G0283539.
GeneIDi8624140.
KEGGiddi:DDB_G0283539.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36937 mRNA. Translation: AAB87719.1.
AAFI02000055 Genomic DNA. Translation: EAL65647.1.
RefSeqiXP_639010.1. XM_633918.1.

3D structure databases

ProteinModelPortaliQ23858.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ23858. 1 interactor.
STRINGi44689.DDB0191384.

Proteomic databases

PaxDbiQ23858.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL65647; EAL65647; DDB_G0283539.
GeneIDi8624140.
KEGGiddi:DDB_G0283539.

Organism-specific databases

dictyBaseiDDB_G0283539. crtA.

Phylogenomic databases

eggNOGiKOG0674. Eukaryota.
ENOG410XRR7. LUCA.
InParanoidiQ23858.
KOiK08057.
OMAiDICGSST.
PhylomeDBiQ23858.

Enzyme and pathway databases

ReactomeiR-DDI-901042. Calnexin/calreticulin cycle.

Miscellaneous databases

PROiQ23858.

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009169. Calreticulin.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 2 hits.
[Graphical view]
PIRSFiPIRSF002356. Calreticulin. 1 hit.
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCALR_DICDI
AccessioniPrimary (citable) accession number: Q23858
Secondary accession number(s): Q54QX4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 4, 2007
Last modified: November 2, 2016
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.