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Q23858

- CALR_DICDI

UniProt

Q23858 - CALR_DICDI

Protein

Calreticulin

Gene

crtA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 3 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei109 – 1091CarbohydrateBy similarity
    Binding sitei111 – 1111CarbohydrateBy similarity
    Binding sitei128 – 1281CarbohydrateBy similarity
    Binding sitei135 – 1351CarbohydrateBy similarity
    Binding sitei318 – 3181CarbohydrateBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: dictyBase

    GO - Biological processi

    1. phagocytosis, engulfment Source: dictyBase
    2. protein folding Source: InterPro
    3. response to other organism Source: dictyBase

    Keywords - Molecular functioni

    Chaperone

    Keywords - Ligandi

    Calcium, Lectin, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calreticulin
    Gene namesi
    Name:crtA
    ORF Names:DDB_G0283539
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0283539. crtA.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: dictyBase
    2. endoplasmic reticulum lumen Source: dictyBase
    3. phagocytic vesicle Source: dictyBase

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 424405CalreticulinPRO_0000004181Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi105 ↔ 137By similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PRIDEiQ23858.

    Interactioni

    Protein-protein interaction databases

    IntActiQ23858. 1 interaction.
    STRINGi44689.DDB_0191384.

    Structurei

    3D structure databases

    ProteinModelPortaliQ23858.
    SMRiQ23858. Positions 205-303.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati191 – 202121-1Add
    BLAST
    Repeati210 – 221121-2Add
    BLAST
    Repeati227 – 238121-3Add
    BLAST
    Repeati246 – 256111-4Add
    BLAST
    Repeati260 – 270112-1Add
    BLAST
    Repeati274 – 284112-2Add
    BLAST
    Repeati288 – 298112-3Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni191 – 256664 X 12 AA approximate repeatsAdd
    BLAST
    Regioni260 – 298393 X 11 AA approximate repeatsAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi421 – 4244Prevents secretion from ERPROSITE-ProRule annotation

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi353 – 42068Asp/Glu/Lys-richAdd
    BLAST

    Domaini

    Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity By similarity.By similarity
    The interaction with glycans occurs through a binding site in the globular lectin domain.By similarity
    The zinc binding sites are localized to the N-domain.By similarity

    Sequence similaritiesi

    Belongs to the calreticulin family.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG305105.
    KOiK08057.
    OMAiSEYSIMF.
    PhylomeDBiQ23858.

    Family and domain databases

    Gene3Di2.10.250.10. 1 hit.
    2.60.120.200. 1 hit.
    InterProiIPR001580. Calret/calnex.
    IPR018124. Calret/calnex_CS.
    IPR009169. Calreticulin.
    IPR009033. Calreticulin/calnexin_P_dom.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    [Graphical view]
    PANTHERiPTHR11073. PTHR11073. 1 hit.
    PfamiPF00262. Calreticulin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002356. Calreticulin. 1 hit.
    PRINTSiPR00626. CALRETICULIN.
    SUPFAMiSSF49899. SSF49899. 1 hit.
    SSF63887. SSF63887. 1 hit.
    PROSITEiPS00804. CALRETICULIN_2. 1 hit.
    PS00805. CALRETICULIN_REPEAT. 1 hit.
    PS00014. ER_TARGET. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q23858-1 [UniParc]FASTAAdd to Basket

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    MRLLLCLIFL VFVFNFALST VHFKDTFDND WESRWVVSDW HKEDGKSGKL    50
    VHTAGKWFGD ENQKGIQTSE DARFYAVSAK FPSFSNKGKD LVLQYTVKNE 100
    QKVDCGGSYI KLLPSKLDQS AFDGESEYSI MFGPDVCGAS KRVHVILNYK 150
    GKNHLIKKEI NKVETDQLTH QYTLVISPDN TYKVLVDNKE IQAGNLADDW 200
    ELLPSKQIKD PKQSKPVDWV DVKEIDDPED VKPAGHDDIP ASIVDPEAVK 250
    PEDWNEEDDG EWEAPTIANP EYKGEWKAKK IPNPEYKGEW VHPLIDNPEY 300
    AEDNELYLFN DLGAIGFELW QVKSGSIFNN MIVTDSVEEA KDFSEKTFVA 350
    NQEAEKKMFD DLEAAKAEER KKADEKLAAE KAAEKEAEEA DEEEEEVAEE 400
    DLVKTDDKKE EVKKSTKKVD HDEL 424
    Length:424
    Mass (Da):48,365
    Last modified:December 4, 2007 - v3
    Checksum:iEAF3D804F96E4AEC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti183 – 1831K → N in AAB87719. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U36937 mRNA. Translation: AAB87719.1.
    AAFI02000055 Genomic DNA. Translation: EAL65647.1.
    RefSeqiXP_639010.1. XM_633918.1.

    Genome annotation databases

    EnsemblProtistsiDDB0191384; DDB0191384; DDB_G0283539.
    GeneIDi8624140.
    KEGGiddi:DDB_G0283539.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U36937 mRNA. Translation: AAB87719.1 .
    AAFI02000055 Genomic DNA. Translation: EAL65647.1 .
    RefSeqi XP_639010.1. XM_633918.1.

    3D structure databases

    ProteinModelPortali Q23858.
    SMRi Q23858. Positions 205-303.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q23858. 1 interaction.
    STRINGi 44689.DDB_0191384.

    Proteomic databases

    PRIDEi Q23858.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0191384 ; DDB0191384 ; DDB_G0283539 .
    GeneIDi 8624140.
    KEGGi ddi:DDB_G0283539.

    Organism-specific databases

    dictyBasei DDB_G0283539. crtA.

    Phylogenomic databases

    eggNOGi NOG305105.
    KOi K08057.
    OMAi SEYSIMF.
    PhylomeDBi Q23858.

    Miscellaneous databases

    PROi Q23858.

    Family and domain databases

    Gene3Di 2.10.250.10. 1 hit.
    2.60.120.200. 1 hit.
    InterProi IPR001580. Calret/calnex.
    IPR018124. Calret/calnex_CS.
    IPR009169. Calreticulin.
    IPR009033. Calreticulin/calnexin_P_dom.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    [Graphical view ]
    PANTHERi PTHR11073. PTHR11073. 1 hit.
    Pfami PF00262. Calreticulin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002356. Calreticulin. 1 hit.
    PRINTSi PR00626. CALRETICULIN.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    SSF63887. SSF63887. 1 hit.
    PROSITEi PS00804. CALRETICULIN_2. 1 hit.
    PS00805. CALRETICULIN_REPEAT. 1 hit.
    PS00014. ER_TARGET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Mueller-Taubenberger A., Gerisch G.
      Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: AX3.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "Identification of novel centrosomal proteins in Dictyostelium discoideum by comparative proteomic approaches."
      Reinders Y., Schulz I., Graef R., Sickmann A.
      J. Proteome Res. 5:589-598(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    4. "Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
      Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
      Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: AX2.

    Entry informationi

    Entry nameiCALR_DICDI
    AccessioniPrimary (citable) accession number: Q23858
    Secondary accession number(s): Q54QX4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 108 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3