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Protein

Alpha-amylase 4N

Gene

Amy4N

Organism
Drosophila ananassae (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
  • chlorideBy similarityNote: Binds 1 Cl- ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi117CalciumBy similarity1
Metal bindingi166Calcium; via carbonyl oxygenBy similarity1
Metal bindingi175CalciumBy similarity1
Binding sitei203ChlorideBy similarity1
Active sitei205NucleophileBy similarity1
Metal bindingi209Calcium; via carbonyl oxygenBy similarity1
Active sitei242Proton donorBy similarity1
Binding sitei305ChlorideBy similarity1
Sitei307Transition state stabilizerBy similarity1
Binding sitei344ChlorideBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Chloride, Metal-binding

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase 4N (EC:3.2.1.1By similarity)
Gene namesi
Name:Amy4N
ORF Names:GF13835
OrganismiDrosophila ananassae (Fruit fly)
Taxonomic identifieri7217 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000007801 Componenti: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0259988. Dana\Amy4N.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000000136220 – 495Alpha-amylase 4NAdd BLAST476

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi47 ↔ 103By similarity
Disulfide bondi154 ↔ 168By similarity
Disulfide bondi377 ↔ 383By similarity
Disulfide bondi449 ↔ 461By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi7217.FBpp0117027.

Structurei

3D structure databases

ProteinModelPortaliQ23834.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2212. Eukaryota.
COG0366. LUCA.
InParanoidiQ23834.
KOiK01176.
OMAiSQVRDCR.
PhylomeDBiQ23834.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q23834-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGLTKSLVC LALLAVANAQ FNTNYASGRS GMVHLFEWKW DDIASECENF
60 70 80 90 100
LGPNGFAGVQ VSPVNENAVK DSRPWWERYQ PISYKLVTRS GNEEQFASMV
110 120 130 140 150
RRCNNAGVRI YVDVVFNHMA ADGGTYGTGG STASPSSKSY PGVPYSSLDF
160 170 180 190 200
NPTCAISNYN DANQVRNCEL VGLRDLNQGN SYVQEKVAEF LNHLIDLGVA
210 220 230 240 250
GFRVDAAKHM WPADLGTIYG RLKTLNTDHG FQSGAKAYIV QEVIDMGGEA
260 270 280 290 300
ISKSEYTGLG AITEFRHSDS IGKVFRGKNQ LKYLTNWGTA WGFAASDRSL
310 320 330 340 350
VFVDNHDNQR GHGAGGADVL TYKVAKQYKM ASAFMLAHPF GTPRVMSSFS
360 370 380 390 400
FSDTDQGPPT TDGHNIASPV FNSDNSCSGG WVCEHRWRQI YNMVAFRNTV
410 420 430 440 450
GSDEIQNWWD NGSNQIAFSR GGKGFVAFNN DNYDLNSSLQ TGLPAGTYCD
460 470 480 490
VISGMKNGSS CTGKTVTVGS DGRANISIGS SEDDGVLAIH VSAKL
Length:495
Mass (Da):53,781
Last modified:November 1, 1998 - v2
Checksum:i529B3E61F00A41C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53477 Genomic DNA. Translation: AAC48343.1.
CH902619 Genomic DNA. Translation: EDV38192.1.
U31121 Genomic DNA. Translation: AAC47352.1.
RefSeqiXP_014764086.1. XM_014908600.1.

Genome annotation databases

EnsemblMetazoaiFBtr0118535; FBpp0117027; FBgn0259988.
GeneIDi26515084.
KEGGidan:Dana_GF27675.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53477 Genomic DNA. Translation: AAC48343.1.
CH902619 Genomic DNA. Translation: EDV38192.1.
U31121 Genomic DNA. Translation: AAC47352.1.
RefSeqiXP_014764086.1. XM_014908600.1.

3D structure databases

ProteinModelPortaliQ23834.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7217.FBpp0117027.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0118535; FBpp0117027; FBgn0259988.
GeneIDi26515084.
KEGGidan:Dana_GF27675.

Organism-specific databases

FlyBaseiFBgn0259988. Dana\Amy4N.

Phylogenomic databases

eggNOGiKOG2212. Eukaryota.
COG0366. LUCA.
InParanoidiQ23834.
KOiK01176.
OMAiSQVRDCR.
PhylomeDBiQ23834.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAM4N_DROAN
AccessioniPrimary (citable) accession number: Q23834
Secondary accession number(s): B3MBP6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: November 1, 1998
Last modified: October 5, 2016
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.