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Protein

Alpha-amylase 4N

Gene

Amy4N

Organism
Drosophila ananassae (Fruit fly)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
  • chlorideBy similarityNote: Binds 1 Cl- ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi117CalciumBy similarity1
Metal bindingi166Calcium; via carbonyl oxygenBy similarity1
Metal bindingi175CalciumBy similarity1
Binding sitei203ChlorideBy similarity1
Active sitei205NucleophileBy similarity1
Metal bindingi209Calcium; via carbonyl oxygenBy similarity1
Active sitei242Proton donorBy similarity1
Binding sitei305ChlorideBy similarity1
Sitei307Transition state stabilizerBy similarity1
Binding sitei344ChlorideBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCalcium, Chloride, Metal-binding

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase 4N (EC:3.2.1.1By similarity)
Gene namesi
Name:Amy4N
ORF Names:GF13835
OrganismiDrosophila ananassae (Fruit fly)
Taxonomic identifieri7217 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000007801 Componenti: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0259988 Dana\Amy4N

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000000136220 – 495Alpha-amylase 4NAdd BLAST476

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi47 ↔ 103By similarity
Disulfide bondi154 ↔ 168By similarity
Disulfide bondi377 ↔ 383By similarity
Disulfide bondi449 ↔ 461By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ23834

Expressioni

Gene expression databases

BgeeiFBgn0259988

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi7217.FBpp0117027

Structurei

3D structure databases

ProteinModelPortaliQ23834
SMRiQ23834
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2212 Eukaryota
COG0366 LUCA
InParanoidiQ23834
KOiK01176
OMAiWKCQHAW
PhylomeDBiQ23834

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q23834-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGLTKSLVC LALLAVANAQ FNTNYASGRS GMVHLFEWKW DDIASECENF
60 70 80 90 100
LGPNGFAGVQ VSPVNENAVK DSRPWWERYQ PISYKLVTRS GNEEQFASMV
110 120 130 140 150
RRCNNAGVRI YVDVVFNHMA ADGGTYGTGG STASPSSKSY PGVPYSSLDF
160 170 180 190 200
NPTCAISNYN DANQVRNCEL VGLRDLNQGN SYVQEKVAEF LNHLIDLGVA
210 220 230 240 250
GFRVDAAKHM WPADLGTIYG RLKTLNTDHG FQSGAKAYIV QEVIDMGGEA
260 270 280 290 300
ISKSEYTGLG AITEFRHSDS IGKVFRGKNQ LKYLTNWGTA WGFAASDRSL
310 320 330 340 350
VFVDNHDNQR GHGAGGADVL TYKVAKQYKM ASAFMLAHPF GTPRVMSSFS
360 370 380 390 400
FSDTDQGPPT TDGHNIASPV FNSDNSCSGG WVCEHRWRQI YNMVAFRNTV
410 420 430 440 450
GSDEIQNWWD NGSNQIAFSR GGKGFVAFNN DNYDLNSSLQ TGLPAGTYCD
460 470 480 490
VISGMKNGSS CTGKTVTVGS DGRANISIGS SEDDGVLAIH VSAKL
Length:495
Mass (Da):53,781
Last modified:November 1, 1998 - v2
Checksum:i529B3E61F00A41C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53477 Genomic DNA Translation: AAC48343.1
CH902619 Genomic DNA Translation: EDV38192.1
U31121 Genomic DNA Translation: AAC47352.1
RefSeqiXP_014764086.1, XM_014908600.1

Genome annotation databases

EnsemblMetazoaiFBtr0384776; FBpp0344793; FBgn0274898
GeneIDi26515084
KEGGidan:Dana_GF27675

Similar proteinsi

Entry informationi

Entry nameiAM4N_DROAN
AccessioniPrimary (citable) accession number: Q23834
Secondary accession number(s): B3MBP6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 105 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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