Q23808 (AQP_CICVR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aquaporin AQPcic

Gene names

Name:	AQP
Synonyms:	CIC

Organism

Cicadella viridis (Green leafhopper)

Taxonomic identifier

36150 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Paraneoptera › Hemiptera › Euhemiptera › Clypeorrhyncha › Membracoidea › Cicadellidae › Cicadellinae › Cicadellini › Cicadella

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Forms a water-specific channel. May be involved in the transfer of excess sap dietary water from the initial midgut to the terminal midgut and the proximal part of the malpighian tubules.
Subunit structure	Homotetramer.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Filter chamber epithelium in the digestive tract. Absent from midgut.
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Repeat Transmembrane Transmembrane helix
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	Aquaporin AQPcic		PRO_0000063975
Regions
	Topological domain	1 – 29	29	Cytoplasmic Potential
	Transmembrane	30 – 50	21	Helical; Name=1; Potential
	Topological domain	51 – 60	10	Extracellular Potential
	Transmembrane	61 – 81	21	Helical; Name=2; Potential
	Topological domain	82 – 103	22	Cytoplasmic Potential
	Transmembrane	104 – 124	21	Helical; Name=3; Potential
	Topological domain	125 – 141	17	Extracellular Potential
	Transmembrane	142 – 162	21	Helical; Name=4; Potential
	Topological domain	163 – 172	10	Cytoplasmic Potential
	Transmembrane	173 – 193	21	Helical; Name=5; Potential
	Topological domain	194 – 215	22	Extracellular Potential
	Transmembrane	216 – 236	21	Helical; Name=6; Potential
	Topological domain	237 – 255	19	Cytoplasmic Potential
	Motif	85 – 87	3	NPA 1
	Motif	201 – 203	3	NPA 2
Experimental info
	Sequence conflict	120 – 146	27	ILKVI…VTPPM → PQGNNPCRVQGHSLYDESRP RCDPAH in CAA54921. Ref.2
	Sequence conflict	165 – 174	10	VCDDRRKNLG → LRRPPQEPA in CAA54921. Ref.2
	Sequence conflict	180	1	A → R in CAA54921. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q23808 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 66093CF3D1253B1D	FASTA	255	26,885
	10 20 30 40 50 60 MAADKSVDNT KKIIGIDDIT DTKTIWRCLA AELIGTLLLV LIGTGSCTGV QISEGDVVVR 70 80 90 100 110 120 IALTFGFIIA TMVQCIGHVS GCHINPAVTC GLLVTGHISI LKAIFYIIVQ CVGAIAGSAI 130 140 150 160 170 180 LKVITPAEFR GTLCMTSLAP GVTPPMGFLV EACITFVLIL LVQSVCDDRR KNLGNAAPVA 190 200 210 220 230 240 VGLAITCCHL AAIKYTGSSM NPARSFGPAV NGDDNWANHW VYWAGPIVGG VVAGITYRAL 250 FRARKPEEEA SSYDF

References

[1]

"Molecular cloning and characterization of an insect aquaporin. Functional comparison with aquaporin 1." Le Caherec F., Deschamps S., Delamarche C., Pellerin I., Bonnec G., Guillam M.-T., Thomas D., Gouranton J., Hubert J.-F. Eur. J. Biochem. 241:707-715(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 245-255. Tissue: Filter chamber.

[2]

"Structural analysis of a MIP family protein from the digestive tract of Cicadella viridis." Beuron F., Le Caherec F., Guillam M.-T., Cavalier A., Garret A., Tassan J.-P., Delamarche C., Schultz P., Mallouh V., Rolland J.-P., Hubert J.-F., Gouranton J., Thomas D. J. Biol. Chem. 270:17414-17422(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 85-205. Tissue: Filter chamber.

Web resources

Protein Spotlight

Liquid states - Issue 36 of July 2003

Cross-references

Sequence databases
EMBL GenBank DDBJ	X97159 mRNA. Translation: CAA65799.1. X77957 mRNA. Translation: CAA54921.1.
3D structure databases
ProteinModelPortal	Q23808.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.20.1080.10. 1 hit.
InterPro	IPR023271. Aquaporin-like. IPR000425. MIP. [Graphical view]
PANTHER	PTHR19139. PTHR19139. 1 hit.
Pfam	PF00230. MIP. 1 hit. [Graphical view]
PRINTS	PR00783. MINTRINSICP.
SUPFAM	SSF81338. MIP. 1 hit.
TIGRFAMs	TIGR00861. MIP. 1 hit.
PROSITE	PS00221. MIP. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AQP_CICVR

Accession

Primary (citable) accession number: Q23808
Secondary accession number(s): Q23816

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 3, 2003
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)