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Protein

Pyruvate kinase

Gene

pyk-2

Organism
Caenorhabditis elegans
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 2 (gpd-2), Glyceraldehyde-3-phosphate dehydrogenase 4 (gpd-4), Glyceraldehyde-3-phosphate dehydrogenase 3 (gpd-3), Glyceraldehyde-3-phosphate dehydrogenase 1 (gpd-1)
  2. Probable phosphoglycerate kinase (pgk-1)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (ipgm-1)
  4. Enolase (enol-1)
  5. Pyruvate kinase (pyk-1), Pyruvate kinase (pyk-1), Pyruvate kinase (pyk-2), Pyruvate kinase (pyk-2), Pyruvate kinase (pyk-1), Pyruvate kinase (pyk-2), Pyruvate kinase (pyk-1), Pyruvate kinase (pyk-1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, PyruvateImported

Enzyme and pathway databases

ReactomeiR-CEL-70171. Glycolysis.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinaseUniRule annotation (EC:2.7.1.40UniRule annotation)
Gene namesi
Name:pyk-2Imported
ORF Names:CELE_ZK593.1Imported, ZK593.1Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiZK593.1a; CE24731; WBGene00014001; pyk-2.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: WormBase
  • sarcomere Source: WormBase
  • striated muscle dense body Source: WormBase
Complete GO annotation...

PTM / Processingi

Proteomic databases

EPDiQ23539.
PaxDbiQ23539.
PRIDEiQ23539.

Expressioni

Gene expression databases

ExpressionAtlasiQ23539. baseline.

Interactioni

Protein-protein interaction databases

IntActiQ23539. 3 interactions.
MINTiMINT-6669282.
STRINGi6239.ZK593.1a.

Structurei

3D structure databases

ProteinModelPortaliQ23539.
SMRiQ23539. Positions 16-512.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 378345PKInterPro annotationAdd
BLAST
Domaini395 – 512118PK_CInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the pyruvate kinase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2323. Eukaryota.
COG0469. LUCA.
GeneTreeiENSGT00390000008859.
HOGENOMiHOG000021559.
InParanoidiQ23539.
OMAiRLTVAWG.
OrthoDBiEOG78M01Q.
PhylomeDBiQ23539.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q23539-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYQDDHIGSE HSSATTNISH LCGLRISERP QKTRKTGVIC TIGPACSDVE
60 70 80 90 100
TLRKMINTGM NIARLNFSHG THEAHAATIK TIREAAENAP FPVAIALDTK
110 120 130 140 150
GPEIRTGMFA NNMKEVQLEN GKSVRVSTDP SMEFAATSSH IYADYRNLPK
160 170 180 190 200
VVQPGSRIYI DDGLISLIVE SCEETAVICT IENGGALGTR KGVNLPGTIV
210 220 230 240 250
DLPAVTSKDI EDLLFGVEQG VDIIFASFIR NADGIHKIRQ VLGEKGKHIY
260 270 280 290 300
IIAKIESEDG VTNCDEIIEA SDGVMVARGD LGIEIPAEKV FLAQKMLIAK
310 320 330 340 350
CNLAGKPVIC ATQMLESMIT KPRPTRAECS DVANAVLDGV DCVMLSGETA
360 370 380 390 400
KGDYPVEALA IMHNICKEAE SAFFHMKHFE ELILHTKKPT GMTHTTAIAA
410 420 430 440 450
VSATITCRAV AIILITTTGK TARLCSRYRP PVPIITVSRD ERISRQLHLH
460 470 480 490 500
RGIFPVYYPK GRIDEWDVDV EERVQYGVNL GKTRGFIHLG DPLIVITGWK
510
QGAGFTNTMR IVVAT
Length:515
Mass (Da):56,159
Last modified:May 1, 2000 - v2
Checksum:i6D7B8BE7E8FA2570
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284604 Genomic DNA. Translation: CAA93424.2.
PIRiF88823.
T27928.
RefSeqiNP_001255515.1. NM_001268586.1.
UniGeneiCel.11603.

Genome annotation databases

EnsemblMetazoaiZK593.1a; ZK593.1a; WBGene00014001.
GeneIDi177984.
KEGGicel:CELE_ZK593.1.
UCSCiZK593.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284604 Genomic DNA. Translation: CAA93424.2.
PIRiF88823.
T27928.
RefSeqiNP_001255515.1. NM_001268586.1.
UniGeneiCel.11603.

3D structure databases

ProteinModelPortaliQ23539.
SMRiQ23539. Positions 16-512.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ23539. 3 interactions.
MINTiMINT-6669282.
STRINGi6239.ZK593.1a.

Proteomic databases

EPDiQ23539.
PaxDbiQ23539.
PRIDEiQ23539.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiZK593.1a; ZK593.1a; WBGene00014001.
GeneIDi177984.
KEGGicel:CELE_ZK593.1.
UCSCiZK593.1. c. elegans.

Organism-specific databases

CTDi177984.
WormBaseiZK593.1a; CE24731; WBGene00014001; pyk-2.

Phylogenomic databases

eggNOGiKOG2323. Eukaryota.
COG0469. LUCA.
GeneTreeiENSGT00390000008859.
HOGENOMiHOG000021559.
InParanoidiQ23539.
OMAiRLTVAWG.
OrthoDBiEOG78M01Q.
PhylomeDBiQ23539.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.
ReactomeiR-CEL-70171. Glycolysis.

Gene expression databases

ExpressionAtlasiQ23539. baseline.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    Caenorhabditis elegans Sequencing Consortium
    Sulson J.E., Waterston R.
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2Imported.

Entry informationi

Entry nameiQ23539_CAEEL
AccessioniPrimary (citable) accession number: Q23539
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: May 1, 2000
Last modified: July 6, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.