Reviewed, UniProtKB/Swiss-Prot Q23500 (ACOC_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable cytoplasmic aconitate hydratase
      Short name=Aconitase
    EC=4.2.1.3
Alternative name(s):
    Citrate hydro-lyase
    Gex-3-interacting protein 22

Gene names

Name:	aco-1
Synonyms:	gei-22
ORF Names:	ZK455.1

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	887 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Citrate = isocitrate.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Subunit structure	Interacts with gex-3. Ref.3
Subcellular location	Cytoplasm. Ref.2
Sequence similarities	Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW aconitate hydratase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ikb-1	Q9U3S0	1	EBI-314193,EBI-314119

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 887	887	Probable cytoplasmic aconitate hydratase		PRO_0000076650
Sites
	Metal binding	436	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	502	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	505	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q23500-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: AB53EF890EBC88C4	FASTA	887	96,660
	10 20 30 40 50 60 MAFNNLIRNL AIGDNVYKYF DLNGLNDARY NELPISIKYL LEAAVRHCDE FHVLKKDVET 70 80 90 100 110 120 ILDWKNSQRN QAEIPFKPAR VILQDFTGVP AVVDLAAMRD AVQNMGADPA KINPVCPVDL 130 140 150 160 170 180 VIDHSVQVDH YGNLEALAKN QSIEFERNRE RFNFLKWGSK AFDNLLIVPP GSGIVHQVNL 190 200 210 220 230 240 EYLARTVFVG KDGVLYPDSV VGTDSHTTMI DGSGVLGWGV GGIEAEAVML GQPISMVIPE 250 260 270 280 290 300 VIGYELVGTL SDTVTSTDLV LTITKNLRDL GVVGKFVEFF GTGVASLSIA DRATIANMCP 310 320 330 340 350 360 EYGATIGFFP VDSRTIDYLT QTGRDTDYTQ RVEQYLKSVG MFVNFTDDSY RPTYTTTLKL 370 380 390 400 410 420 DLGSVVPSVS GPKRPHDRVE LASLAQDFSK GLTDKISFKA FGLKPEDATK SVTITNHGRT 430 440 450 460 470 480 AELTHGSVVI AAITSCTNTS NPSVMLAAGL VAKKAVELGL NVQPYVKTSL SPGSGVVTKY 490 500 510 520 530 540 LEASGLLPYL EKIGFNIAGY GCMTCIGNSG PLDEPVTKAI EENNLVVAGV LSGNRNFEGR 550 560 570 580 590 600 IHPHVRANYL ASPPLAVLYS IIGNVNVDIN GVLAVTPDGK EIRLADIWPT RKEVAKFEEE 610 620 630 640 650 660 FVKPQFFREV YANIELGSTE WQQLECPAVK LYPWDDASTY IKKVPFFDGM TSELPSQSDI 670 680 690 700 710 720 VNAHVLLNLG DSVTTDHISP AGSISKTSPA ARFLAGRGVT PRDFNTYGAR RGNDEIMARG 730 740 750 760 770 780 TFANIRLVNK LASKVGPITL HVPSGEELDI FDAAQKYKDA GIPAIILAGK EYGCGSSRDW 790 800 810 820 830 840 AAKGPFLQGV KAVIAESFER IHRSNLIGMG IIPFQYQAGQ NADSLGLTGK EQFSIGVPDD 850 860 870 880 LKPGQLIDVN VSNGSVFQVI CRFDTEVELT YYRNGGILQY MIRKLIQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Cytosolic aconitase and ferritin are regulated by iron in Caenorhabditis elegans." Gourley B.L., Parker S.B., Jones B.J., Zumbrennen K.B., Leibold E.A. J. Biol. Chem. 278:3227-3234(2003) [PubMed: 12438312] [Abstract] Cited for: CHARACTERIZATION, SUBCELLULAR LOCATION.
[3]	"Isolation of the interacting molecules with GEX-3 by a novel functional screening." Tsuboi D., Qadota H., Kasuya K., Amano M., Kaibuchi K. Biochem. Biophys. Res. Commun. 292:697-701(2002) [PubMed: 11922622] [Abstract] Cited for: INTERACTION WITH GEX-3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z66567 Genomic DNA. Translation: CAA91491.1.
PIR	T27868.
RefSeq	NP_509898.1.
UniGene	Cel.17233
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:26479N.
IntAct	Q23500. 2 interactions.
STRING	Q23500.
Genome annotation databases
Ensembl	ZK455.1.1; ZK455.1.1; ZK455.1; Caenorhabditis elegans. [Genome view] ZK455.1.2; ZK455.1.2; ZK455.1; Caenorhabditis elegans. [Genome view]
GeneID	181324.
KEGG	cel:ZK455.1.
UCSC	ZK455.1.1. c. elegans.
Organism-specific databases
CTD	181324.
WormBase	WBGene00000040. aco-1.
WormPep	ZK455.1. CE03812. [WorfDB]
Phylogenomic databases
OMA	DFTQVVE.
Enzyme and pathway databases
BRENDA	4.2.1.3. 672.
Gene expression databases
ArrayExpress	Q23500.
Family and domain databases
InterPro	IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015937. Aconitase-like_core. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR006249. Aconitase/Fe_reg_prot_2. IPR015934. Aconitase/Fe_reg_prot_2/AcnD. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
Gene3D	G3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 2 hits. G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit. G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit.
PANTHER	PTHR11670. Aconitase-like_core. 1 hit. PTHR11670:SF1. Aconitase/Fe_reg_prot_2/AcnD. 1 hit.
Pfam	PF00330. Aconitase. 1 hit. PF00694. Aconitase_C. 1 hit. [Graphical view]
PRINTS	PR00415. ACONITASE.
ProDom	PD000511. Aconitase_N. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01341. aconitase_1. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	913448.

Entry information

Entry name

ACOC_CAEEL

Accession

Primary (citable) accession number: Q23500

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project