Q23498 (ASM2_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sphingomyelin phosphodiesterase 2
EC=3.1.4.12

Alternative name(s):
Acid sphingomyelinase 2

Gene names

Name:	asm-2
ORF Names:	ZK455.4

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	618 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Converts sphingomyelin to ceramide. Ref.1
Catalytic activity	Sphingomyelin + H2O = N-acylsphingosine + choline phosphate. Ref.1
Subcellular location	Secreted Ref.1.
Developmental stage	Preferentially expressed in post-embryonic development. Ref.1
Miscellaneous	Fully active upon secretion independent of zinc ions. Ref.1 There are two types of sphingomyelinases: asm (acid), and nsm (neutral). Only acid sphingomyelinase has been found in worms. Ref.1 UniProtKB P17405
Sequence similarities	Belongs to the acid sphingomyelinase family. UniProtKB P17405 Contains 1 saposin B-type domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ceramide biosynthetic process Inferred from direct assay Ref.1. Source: UniProtKB sphingomyelin catabolic process Inferred from direct assay Ref.1. Source: UniProtKB
Cellular component	extracellular region Inferred from direct assay Ref.1. Source: UniProtKB intracellular Inferred from direct assay Ref.1. Source: WormBase
Molecular function	hydrolase activity, acting on glycosyl bonds Inferred from electronic annotation. Source: UniProtKB-KW sphingomyelin phosphodiesterase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 618	596	Sphingomyelin phosphodiesterase 2		PRO_0000002326
Regions
	Domain	68 – 151	84	Saposin B-type
Amino acid modifications
	Glycosylation	89	1	N-linked (GlcNAc...) Potential
	Glycosylation	159	1	N-linked (GlcNAc...) Potential
	Glycosylation	298	1	N-linked (GlcNAc...) Potential
	Glycosylation	525	1	N-linked (GlcNAc...) Ref.3
	Glycosylation	568	1	N-linked (GlcNAc...) Potential
	Disulfide bond	72 ↔ 147		By similarity
	Disulfide bond	75 ↔ 140		By similarity
	Disulfide bond	103 ↔ 114		By similarity UniProtKB P17405
	Disulfide bond	204 ↔ 216		By similarity UniProtKB P17405
	Disulfide bond	217 ↔ 249		By similarity UniProtKB P17405
	Disulfide bond	387 ↔ 435		By similarity UniProtKB P17405
	Disulfide bond	588 ↔ 594		By similarity UniProtKB P17405
	Disulfide bond	600 ↔ 613		By similarity UniProtKB P17405

Sequences

	Sequence		Length	Mass (Da)
	Q23498 [UniParc]. Last modified June 1, 2003. Version 3. Checksum: CF24C82DC09BA115	FASTA	618	71,925
	10 20 30 40 50 60 MQQPLIILGI GIVLALVSNV ESGVLRKPVD EHEYEKWTNA RGNEAAVPPP KYKMLRYAKK 70 80 90 100 110 120 AINEPENRKM SCLFCTFAVD GVQALIAQNS TDNEIAAFLV NLCDLFDVEQ PHVCKNIIYA 130 140 150 160 170 180 FKDEVVFVLE RSVFTPEEIC GAFIANCGHS DKPLTHMWNI TIPGGKPPIK PWPKIPDNKP 190 200 210 220 230 240 TFKVLHLSDI HIDHQYVVGT EAYCQLDSAL GTYAMCCRDY SQDSQGAPTN LKDKPIYVPA 250 260 270 280 290 300 GPWGMPYLCD LPYQTFESAM KHISKTFKDL DYIIITGDFE AHDSWDYTED LTRENMNNMT 310 320 330 340 350 360 NVFLEYFPGV PVYVSIGNHE GVPQDAMAPH TMPEYDTRGP QWLYKIMSEM WSHWIPQEAL 370 380 390 400 410 420 DTVQYRASYA VYPKPGLKLI SLNTIYCSEF NFYLYVNEVD PDATLEWLIE ELQDSENKGE 430 440 450 460 470 480 LVHIISHIPP GDNYCLKGWS WNFFEIVKRY ENTIAQMFYG HTHYDQFMVY YDMDDPNRRP 490 500 510 520 530 540 FHFNWISPSL TTYDWLNPAY RIYEIDGGYE GATYTVKDAK TYFANVTEAN MKNKEPEWVL 550 560 570 580 590 600 SYDTREHYQM ADFSPQSWSD LSDKLWTNTT LFRDYVRLYY RNHYNNECYT DYKCRYTFVC 610 DIKKGRSYDE SFCDHLTK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Caenorhabditis elegans contains two distinct acid sphingomyelinases." Lin X., Hengartner M.O., Kolesnick R. J. Biol. Chem. 273:14374-14379(1998) [PubMed: 9603947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins." Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T. Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed: 17761667] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-525, MASS SPECTROMETRY. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z66567 Genomic DNA. Translation: CAA91493.2.
PIR	T27869.
RefSeq	NP_509894.2. NM_077493.3.
UniGene	Cel.16898.
3D structure databases
ProteinModelPortal	Q23498.
SMR	Q23498. Positions 70-148.
ModBase	Search...
Protein-protein interaction databases
STRING	Q23498.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	ZK455.4.1; ZK455.4.1; ZK455.4.
GeneID	181323.
KEGG	cel:ZK455.4.
UCSC	ZK455.4. c. elegans.
Organism-specific databases
CTD	181323.
WormBase	ZK455.4; CE31674; WBGene00000212; asm-2.
Phylogenomic databases
eggNOG	meNOG05776.
GeneTree	EMGT00050000009609.
HOGENOM	HBG390117.
InParanoid	Q23498.
OMA	DMVYWTG.
PhylomeDB	Q23498.
Gene expression databases
ArrayExpress	Q23498.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR007856. SapB_1. IPR011001. Saposin-like. IPR008139. SaposinB. IPR011160. Sphingomy_PDE. [Graphical view]
Gene3D	G3DSA:1.10.225.10. Saposin_like. 1 hit.
KO	K12350.
Pfam	PF00149. Metallophos. 1 hit. PF05184. SapB_1. 1 hit. [Graphical view]
PIRSF	PIRSF000948. Sphingomy_PDE. 1 hit.
SMART	SM00741. SapB. 1 hit. [Graphical view]
SUPFAM	SSF47862. Saposin_like. 1 hit.
PROSITE	PS50015. SAP_B. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	913444.

Entry information

Entry name

ASM2_CAEEL

Accession

Primary (citable) accession number: Q23498

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2003
Last sequence update:	June 1, 2003
Last modified:	November 16, 2011
This is version 75 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project