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Protein

Peroxidase mlt-7

Gene

mlt-7

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an essential role in cuticle biogenesis. Required in combination with bli-3 for correct formation of cross-links in cuticle collagens.1 Publication

Catalytic activityi

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei271Proton acceptorPROSITE-ProRule annotation1
Metal bindingi272CalciumPROSITE-ProRule annotation1
Metal bindingi335CalciumPROSITE-ProRule annotation1
Metal bindingi337Calcium; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi339CalciumPROSITE-ProRule annotation1
Metal bindingi341CalciumPROSITE-ProRule annotation1
Sitei396Transition state stabilizerPROSITE-ProRule annotation1
Metal bindingi493Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

  • heme binding Source: InterPro
  • metal ion binding Source: UniProtKB-KW
  • peroxidase activity Source: WormBase

GO - Biological processi

  • cuticle development involved in collagen and cuticulin-based cuticle molting cycle Source: WormBase
  • nematode larval development Source: WormBase
  • peptide cross-linking Source: WormBase
  • post-embryonic body morphogenesis Source: WormBase
  • response to oxidative stress Source: InterPro

Keywordsi

Molecular functionDevelopmental protein, Oxidoreductase, Peroxidase
LigandCalcium, Heme, Iron, Metal-binding

Protein family/group databases

PeroxiBasei4141 CelPxd05

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxidase mlt-71 Publication (EC:1.11.1.7)
Alternative name(s):
Molting defective protein 71 Publication
Short name:
MoLT-71 Publication
Cleaved into the following 2 chains:
Gene namesi
Name:mlt-7Imported
ORF Names:ZK430.8
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiZK430.8 ; CE05084 ; WBGene00022743 ; mlt-7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Gross morphological abnormalities causing larval arrest with associated molt defects and low levels of embryonic lethality. Cuticle function and integrity are also impaired.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000039168225 – 178Sequence analysisAdd BLAST154
ChainiPRO_0000391683179 – 281Peroxidase mlt-7 light chainSequence analysisAdd BLAST103
ChainiPRO_0000391684282 – 724Peroxidase mlt-7 heavy chainSequence analysisAdd BLAST443

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi7N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi181 ↔ 198Sequence analysis
Glycosylationi233N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi284 ↔ 294Sequence analysis
Glycosylationi509N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi588 ↔ 645Sequence analysis
Glycosylationi617N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi686 ↔ 710Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ23490
PaxDbiQ23490
PeptideAtlasiQ23490
PRIDEiQ23490

PTM databases

iPTMnetiQ23490

Expressioni

Tissue specificityi

Expressed in the hypodermal cells, specifically the head and seam/body.1 Publication

Developmental stagei

Expressed in all adult and larval stages. Expression is cyclical and coincides with each of the larval molts, peaking at 12, 18, 24, and 30h post-hatching.1 Publication

Gene expression databases

BgeeiWBGene00022743

Interactioni

Protein-protein interaction databases

STRINGi6239.ZK430.8

Structurei

3D structure databases

ProteinModelPortaliQ23490
SMRiQ23490
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 76ShKTPROSITE-ProRule annotationAdd BLAST35

Sequence similaritiesi

Belongs to the peroxidase family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2408 Eukaryota
ENOG410XPZ3 LUCA
GeneTreeiENSGT00550000074325
HOGENOMiHOG000016084
InParanoidiQ23490
OMAiKVFFASW
OrthoDBiEOG091G02K8
PhylomeDBiQ23490

Family and domain databases

Gene3Di1.10.640.10, 1 hit
InterProiView protein in InterPro
IPR010255 Haem_peroxidase
IPR019791 Haem_peroxidase_animal
IPR037120 Haem_peroxidase_sf
IPR003582 ShKT_dom
PfamiView protein in Pfam
PF03098 An_peroxidase, 1 hit
PF01549 ShK, 1 hit
PRINTSiPR00457 ANPEROXIDASE
SMARTiView protein in SMART
SM00254 ShKT, 1 hit
SUPFAMiSSF48113 SSF48113, 1 hit
PROSITEiView protein in PROSITE
PS50292 PEROXIDASE_3, 1 hit
PS51670 SHKT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q23490-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRLHRNLSL LFLICILNEY RIESQTLSPP ITDRFKCLTN GCCDHHEWCR
60 70 80 90 100
FWASIGECNA NKDWMTENCQ LACGTCTAPA APLLPVTTTA SSFNGGGFVQ
110 120 130 140 150
TTTQSSGPTT TITIPPSSLT SVTSCERVKD SIAQASELMS ISRLINPVED
160 170 180 190 200
NFGRNMLSID DITRSVPTGC VPQLSDVGVD CRKSLCYHLM YRTLDGTCNN
210 220 230 240 250
LEKPMQGAAF RRFNRHFPAQ YDDGKGEPIS SLNQSRPSAR EANRVMLSSA
260 270 280 290 300
QSVVHDKFNN MMMQWGQFMS HDMSKTTLQP SANCKTCDPV PSKCMPIPIG
310 320 330 340 350
EKDPNLGFKS KQCLKVSRSA PICRVEPREQ LNENTAYIDG SMIYGSSLKD
360 370 380 390 400
LHKFRDGRTG FLRVTRFNNQ NVLPFDQSKC ANKDKCTASF TAGDIRANLF
410 420 430 440 450
IGLSSLHIMF AREHNRIAQK LTELNPTWSG DRVFQEARKI VGAQIQNVLY
460 470 480 490 500
KEYLPKLLGV SFDKVIGPYK GYDTNVDATI ANEFTTSAFR FGHGMIEEFY
510 520 530 540 550
KRVDLSGNNI THGGFFFGDG VFKSGKILFE GGVDPIIRGF MTTAVKRPHR
560 570 580 590 600
MTPAITEKMF GSTDLGSLNI QRGRDHGIPS YNKMRQFCGL KSANTFDDFA
610 620 630 640 650
DMILDRNLRA GLARNYNTTN DVDFYVGSML EDPVIGGLVG TTLSCAIGEQ
660 670 680 690 700
FKRARDGDRF YFENPGIFTR SQMEEIKKSS LSRIICDNAD NFELVSQDAF
710 720
LLPGSNLTPC SKIPKMDLSK WRAL
Length:724
Mass (Da):80,829
Last modified:November 1, 1996 - v1
Checksum:i54986FA3545753AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080155 Genomic DNA Translation: CCD61675.1
PIRiT27858
RefSeqiNP_494777.1, NM_062376.4
UniGeneiCel.8878

Genome annotation databases

EnsemblMetazoaiZK430.8; ZK430.8; WBGene00022743
GeneIDi173775
KEGGicel:CELE_ZK430.8
UCSCiZK430.8 c. elegans

Similar proteinsi

Entry informationi

Entry nameiMLT7_CAEEL
AccessioniPrimary (citable) accession number: Q23490
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 2, 2010
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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