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Q23457 (RBX1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RING-box protein 1

Short name=Rbx1
Alternative name(s):
Ce-rbx-1
Gene names
Name:rbx-1
ORF Names:ZK287.5
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length110 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate By similarity. Essential for meiosis, mitotic chromosomal condensation and cytokinesis. Involved in histone H3 phosphorylation. Ref.2

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Part of SCF complexes, which consist of a SKP1 or a SKP1-related protein, a cullin protein, and a F-box protein By similarity. Interacts with csn-1 and csn-6. Ref.3

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Tissue specificity

Strongly expressed in the gonads of hermaphrodite animals. Ref.2

Developmental stage

Expressed at high levels in eggs and adults, and at low levels in larva. Ref.2

Domain

The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.

Sequence similarities

Belongs to the RING-box family.

Contains 1 RING-type zinc finger.

Caution

It is uncertain whether Met-1 or Met-10 is the initiator.

Sequence caution

The sequence BAB83695.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 110110RING-box protein 1
PRO_0000056016

Regions

Zinc finger44 – 10057RING-type

Sites

Metal binding441Zinc 1 By similarity
Metal binding471Zinc 1 By similarity
Metal binding551Zinc 3 By similarity
Metal binding581Zinc 3 By similarity
Metal binding701Zinc 3 By similarity
Metal binding771Zinc 2 By similarity
Metal binding791Zinc 2 By similarity
Metal binding821Zinc 1 By similarity
Metal binding841Zinc 3 By similarity
Metal binding851Zinc 1 By similarity
Metal binding961Zinc 2 By similarity
Metal binding991Zinc 2 By similarity

Experimental info

Sequence conflict2 – 32AQ → GP in BAB83695. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q23457 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: EFE50F7684B30A56

FASTA11012,760
        10         20         30         40         50         60 
MAQASDSTAM EVEEATNQTV KKRFEVKKWS AVALWAWDIQ VDNCAICRNH IMDLCIECQA 

        70         80         90        100        110 
NQAAGLKDEC TVAWGNCNHA FHFHCISRWL KTRQVCPLDN REWEFQKYGH 

« Hide

References

« Hide 'large scale' references
[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[2]"Caenorhabditis elegans RBX1 is essential for meiosis, mitotic chromosomal condensation and segregation, and cytokinesis."
Sasagawa Y., Urano T., Kohara Y., Takahashi H., Higashitani A.
Genes Cells 8:857-872(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-110, FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[3]"Neddylation and deneddylation of CUL-3 is required to target MEI-1/katanin for degradation at the meiosis-to-mitosis transition in C. elegans."
Pintard L., Kurz T., Glaser S., Willis J.H., Peter M., Bowerman B.
Curr. Biol. 13:911-921(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CSN-1 AND CSN-6.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z70757 Genomic DNA. Translation: CAA94801.1.
AB077287 mRNA. Translation: BAB83695.1. Different initiation.
PIRT27823.
RefSeqNP_505496.1. NM_073095.5.
UniGeneCel.7279.

3D structure databases

ProteinModelPortalQ23457.
SMRQ23457. Positions 21-110.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid44394. 4 interactions.
IntActQ23457. 5 interactions.
MINTMINT-4052036.
STRING6239.ZK287.5.1.

Proteomic databases

PaxDbQ23457.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaZK287.5.1; ZK287.5.1; WBGene00004320.
ZK287.5.2; ZK287.5.2; WBGene00004320.
GeneID179358.
KEGGcel:CELE_ZK287.5.
UCSCZK287.5.1. c. elegans.

Organism-specific databases

CTD179358.
WormBaseZK287.5; CE06614; WBGene00004320; rbx-1.

Phylogenomic databases

eggNOGCOG5194.
GeneTreeENSGT00390000017058.
HOGENOMHOG000171951.
InParanoidQ23457.
KOK03868.
OMAARSVCPL.
OrthoDBEOG7CG721.
PhylomeDBQ23457.

Enzyme and pathway databases

UniPathwayUPA00143.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR024766. Znf_RING_H2.
[Graphical view]
PfamPF12678. zf-rbx1. 1 hit.
[Graphical view]
SMARTSM00184. RING. 1 hit.
[Graphical view]
PROSITEPS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio905060.
PROQ23457.

Entry information

Entry nameRBX1_CAEEL
AccessionPrimary (citable) accession number: Q23457
Secondary accession number(s): Q8WSQ1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase