RING-box protein 1 - Caenorhabditis elegans

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Q23457 (RBX1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 105. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
RING-box protein 1
Short name=Rbx1

Alternative name(s):
Ce-rbx-1

Gene names

Name:	rbx-1
ORF Names:	ZK287.5

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	110 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate By similarity. Essential for meiosis, mitotic chromosomal condensation and cytokinesis. Involved in histone H3 phosphorylation. Ref.2
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Part of SCF complexes, which consist of a SKP1 or a SKP1-related protein, a cullin protein, and a F-box protein By similarity. Interacts with csn-1 and csn-6. Ref.3
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Tissue specificity	Strongly expressed in the gonads of hermaphrodite animals. Ref.2
Developmental stage	Expressed at high levels in eggs and adults, and at low levels in larva. Ref.2
Domain	The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.
Sequence similarities	Belongs to the RING-box family. Contains 1 RING-type zinc finger.
Caution	It is uncertain whether Met-1 or Met-10 is the initiator.
Sequence caution	The sequence BAB83695.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle Cell division Meiosis Mitosis Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	anterior/posterior axis specification, embryo Inferred from mutant phenotype PubMed 15215209. Source: WormBase cell division Inferred from electronic annotation. Source: UniProtKB-KW meiotic anaphase II Inferred from mutant phenotype PubMed 15215209. Source: WormBase mitosis Inferred from electronic annotation. Source: UniProtKB-KW protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-UniPathway sister chromosome movement towards spindle pole involved in meiotic sister chromatid segregation Inferred from mutant phenotype PubMed 15215209. Source: WormBase
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 110

110

RING-box protein 1

PRO_0000056016

Regions

Zinc finger

44 – 100

RING-type

Sites

Metal binding

Zinc 1 By similarity

Metal binding

Zinc 1 By similarity

Metal binding

Zinc 3 By similarity

Metal binding

Zinc 3 By similarity

Metal binding

Zinc 3 By similarity

Metal binding

Zinc 2 By similarity

Metal binding

Zinc 2 By similarity

Metal binding

Zinc 1 By similarity

Metal binding

Zinc 3 By similarity

Metal binding

Zinc 1 By similarity

Metal binding

Zinc 2 By similarity

Metal binding

Zinc 2 By similarity

Experimental info

Sequence conflict

2 – 3

AQ → GP in BAB83695. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q23457 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: EFE50F7684B30A56
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FASTA

110

12,760

        10         20         30         40         50         60 
MAQASDSTAM EVEEATNQTV KKRFEVKKWS AVALWAWDIQ VDNCAICRNH IMDLCIECQA 

        70         80         90        100        110 
NQAAGLKDEC TVAWGNCNHA FHFHCISRWL KTRQVCPLDN REWEFQKYGH

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Caenorhabditis elegans RBX1 is essential for meiosis, mitotic chromosomal condensation and segregation, and cytokinesis." Sasagawa Y., Urano T., Kohara Y., Takahashi H., Higashitani A. Genes Cells 8:857-872(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-110, FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[3]	"Neddylation and deneddylation of CUL-3 is required to target MEI-1/katanin for degradation at the meiosis-to-mitosis transition in C. elegans." Pintard L., Kurz T., Glaser S., Willis J.H., Peter M., Bowerman B. Curr. Biol. 13:911-921(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CSN-1 AND CSN-6.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z70757 Genomic DNA. Translation: CAA94801.1. AB077287 mRNA. Translation: BAB83695.1. Different initiation.
PIR	T27823.
RefSeq	NP_505496.1. NM_073095.5.
3D structure databases
ProteinModelPortal	Q23457.
SMR	Q23457. Positions 21-110.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q23457. 5 interactions.
MINT	MINT-4052036.
STRING	6239.ZK287.5.1.
Proteomic databases
PaxDb	Q23457.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	ZK287.5.1; ZK287.5.1; ZK287.5. ZK287.5.2; ZK287.5.2; ZK287.5.
GeneID	179358.
KEGG	cel:CELE_ZK287.5.
UCSC	ZK287.5.1. c. elegans.
Organism-specific databases
CTD	179358.
WormBase	ZK287.5; CE06614; WBGene00004320; rbx-1.
Phylogenomic databases
eggNOG	COG5194.
GeneTree	ENSGT00390000017058.
HOGENOM	HOG000171951.
InParanoid	Q23457.
KO	K03868.
OMA	NRDWEFQ.
Enzyme and pathway databases
UniPathway	UPA00143.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. IPR024766. Znf_RING_H2. [Graphical view]
Pfam	PF12678. zf-rbx1. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	905060.

Entry information

Entry name

RBX1_CAEEL

Accession

Primary (citable) accession number: Q23457
Secondary accession number(s): Q8WSQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2003
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Other

Entry information

Relevant documents