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Q23457

- RBX1_CAEEL

UniProt

Q23457 - RBX1_CAEEL

Protein

RING-box protein 1

Gene

rbx-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate By similarity. Essential for meiosis, mitotic chromosomal condensation and cytokinesis. Involved in histone H3 phosphorylation.By similarity1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi44 – 441Zinc 1By similarity
    Metal bindingi47 – 471Zinc 1By similarity
    Metal bindingi55 – 551Zinc 3By similarity
    Metal bindingi58 – 581Zinc 3By similarity
    Metal bindingi70 – 701Zinc 3By similarity
    Metal bindingi77 – 771Zinc 2By similarity
    Metal bindingi79 – 791Zinc 2By similarity
    Metal bindingi82 – 821Zinc 1By similarity
    Metal bindingi84 – 841Zinc 3By similarity
    Metal bindingi85 – 851Zinc 1By similarity
    Metal bindingi96 – 961Zinc 2By similarity
    Metal bindingi99 – 991Zinc 2By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri44 – 10057RING-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. zinc ion binding Source: InterPro

    GO - Biological processi

    1. anterior/posterior axis specification, embryo Source: WormBase
    2. mitotic nuclear division Source: UniProtKB-KW
    3. positive regulation of metaphase/anaphase transition of meiotic cell cycle Source: WormBase
    4. protein ubiquitination Source: UniProtKB-UniPathway
    5. sister chromosome movement towards spindle pole involved in meiotic sister chromatid segregation Source: WormBase

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Cell cycle, Cell division, Meiosis, Mitosis, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RING-box protein 1
    Short name:
    Rbx1
    Alternative name(s):
    Ce-rbx-1
    Gene namesi
    Name:rbx-1
    ORF Names:ZK287.5
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome V

    Organism-specific databases

    WormBaseiZK287.5; CE06614; WBGene00004320; rbx-1.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 110110RING-box protein 1PRO_0000056016Add
    BLAST

    Proteomic databases

    PaxDbiQ23457.

    Expressioni

    Tissue specificityi

    Strongly expressed in the gonads of hermaphrodite animals.1 Publication

    Developmental stagei

    Expressed at high levels in eggs and adults, and at low levels in larva.1 Publication

    Interactioni

    Subunit structurei

    Part of SCF complexes, which consist of a SKP1 or a SKP1-related protein, a cullin protein, and a F-box protein By similarity. Interacts with csn-1 and csn-6.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi44394. 4 interactions.
    IntActiQ23457. 5 interactions.
    MINTiMINT-4052036.
    STRINGi6239.ZK287.5.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ23457.
    SMRiQ23457. Positions 21-110.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.

    Sequence similaritiesi

    Belongs to the RING-box family.Curated
    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri44 – 10057RING-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5194.
    GeneTreeiENSGT00390000017058.
    HOGENOMiHOG000171951.
    InParanoidiQ23457.
    KOiK03868.
    OMAiARSVCPL.
    OrthoDBiEOG7CG721.
    PhylomeDBiQ23457.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR024766. Znf_RING_H2.
    [Graphical view]
    PfamiPF12678. zf-rbx1. 1 hit.
    [Graphical view]
    SMARTiSM00184. RING. 1 hit.
    [Graphical view]
    PROSITEiPS50089. ZF_RING_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q23457-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQASDSTAM EVEEATNQTV KKRFEVKKWS AVALWAWDIQ VDNCAICRNH    50
    IMDLCIECQA NQAAGLKDEC TVAWGNCNHA FHFHCISRWL KTRQVCPLDN 100
    REWEFQKYGH 110
    Length:110
    Mass (Da):12,760
    Last modified:November 1, 1996 - v1
    Checksum:iEFE50F7684B30A56
    GO

    Sequence cautioni

    The sequence BAB83695.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2 – 32AQ → GP in BAB83695. (PubMed:14622138)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z70757 Genomic DNA. Translation: CAA94801.1.
    AB077287 mRNA. Translation: BAB83695.1. Different initiation.
    PIRiT27823.
    RefSeqiNP_505496.1. NM_073095.5.
    UniGeneiCel.7279.

    Genome annotation databases

    EnsemblMetazoaiZK287.5.1; ZK287.5.1; WBGene00004320.
    ZK287.5.2; ZK287.5.2; WBGene00004320.
    GeneIDi179358.
    KEGGicel:CELE_ZK287.5.
    UCSCiZK287.5.1. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z70757 Genomic DNA. Translation: CAA94801.1 .
    AB077287 mRNA. Translation: BAB83695.1 . Different initiation.
    PIRi T27823.
    RefSeqi NP_505496.1. NM_073095.5.
    UniGenei Cel.7279.

    3D structure databases

    ProteinModelPortali Q23457.
    SMRi Q23457. Positions 21-110.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 44394. 4 interactions.
    IntActi Q23457. 5 interactions.
    MINTi MINT-4052036.
    STRINGi 6239.ZK287.5.1.

    Proteomic databases

    PaxDbi Q23457.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai ZK287.5.1 ; ZK287.5.1 ; WBGene00004320 .
    ZK287.5.2 ; ZK287.5.2 ; WBGene00004320 .
    GeneIDi 179358.
    KEGGi cel:CELE_ZK287.5.
    UCSCi ZK287.5.1. c. elegans.

    Organism-specific databases

    CTDi 179358.
    WormBasei ZK287.5 ; CE06614 ; WBGene00004320 ; rbx-1.

    Phylogenomic databases

    eggNOGi COG5194.
    GeneTreei ENSGT00390000017058.
    HOGENOMi HOG000171951.
    InParanoidi Q23457.
    KOi K03868.
    OMAi ARSVCPL.
    OrthoDBi EOG7CG721.
    PhylomeDBi Q23457.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    NextBioi 905060.
    PROi Q23457.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR024766. Znf_RING_H2.
    [Graphical view ]
    Pfami PF12678. zf-rbx1. 1 hit.
    [Graphical view ]
    SMARTi SM00184. RING. 1 hit.
    [Graphical view ]
    PROSITEi PS50089. ZF_RING_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    2. "Caenorhabditis elegans RBX1 is essential for meiosis, mitotic chromosomal condensation and segregation, and cytokinesis."
      Sasagawa Y., Urano T., Kohara Y., Takahashi H., Higashitani A.
      Genes Cells 8:857-872(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-110, FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    3. "Neddylation and deneddylation of CUL-3 is required to target MEI-1/katanin for degradation at the meiosis-to-mitosis transition in C. elegans."
      Pintard L., Kurz T., Glaser S., Willis J.H., Peter M., Bowerman B.
      Curr. Biol. 13:911-921(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CSN-1 AND CSN-6.

    Entry informationi

    Entry nameiRBX1_CAEEL
    AccessioniPrimary (citable) accession number: Q23457
    Secondary accession number(s): Q8WSQ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2003
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Caution

    It is uncertain whether Met-1 or Met-10 is the initiator.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3