Reviewed, UniProtKB/Swiss-Prot Q23361 (ALG12_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable dolichyl-P-Man:Man(7)GlcNAc(2)-PP-dolichyl-alpha-1,6-mannosyltransferase
    EC=2.4.1.-
Alternative name(s):
    Mannosyltransferase ALG12 homolog

Gene names

ORF Names:

ZC513.5

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Adds the eighth mannose residue in an alpha-1,6 linkage onto the dolichol-PP-oligosaccharide precursor (dolichol-PP-Man7GlcNAc2) required for protein glycosylation By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 22 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GPI anchor biosynthetic process Inferred from electronic annotation. Source: InterPro dolichol-linked oligosaccharide biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intrinsic to endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro
Molecular function	alpha-1,6-mannosyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	Probable dolichyl-P-Man:Man(7)GlcNAc(2)-PP-dolichyl-alpha-1,6-mannosyltransferase		PRO_0000215783
Regions
	Transmembrane	1 – 21	21	Potential
	Transmembrane	60 – 80	21	Potential
	Transmembrane	86 – 106	21	Potential
	Transmembrane	133 – 153	21	Potential
	Transmembrane	172 – 192	21	Potential
	Transmembrane	198 – 218	21	Potential
	Transmembrane	259 – 279	21	Potential
	Transmembrane	284 – 304	21	Potential
	Transmembrane	309 – 329	21	Potential
	Transmembrane	339 – 359	21	Potential
Amino acid modifications
	Glycosylation	413	1	N-linked (GlcNAc...) Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q23361-1 [UniParc]. Last modified October 1, 2001. Version 2. Checksum: 73BC4F72ADA6B643	FASTA	492	57,150
	10 20 30 40 50 60 MEGTEWIVII VTIIHIILAP GTKVEESFNV QATHDLMFHL PTNLSNYDHS QFPGVVPRTF 70 80 90 100 110 120 IGPISLAILS SPMSFIFRFW AIPKMWQLLL IRATLGLMNA MAFLYFARSV NRKFGRETAM 130 140 150 160 170 180 YLRLIMCTQF HYIFYMSRPL PNTFALILVM IVFERLLEGR YESAVRYATA SVILFRCELV 190 200 210 220 230 240 LLYGPIFLGY MISGRLKVFG FDGAIAIGVR IAAMCLAVSI PIDSYFWGRP LWPEGEVMFF 250 260 270 280 290 300 NVVENRSHEY GTQPFLWYFY SALPRCLLTT TLLVPLGLLV DRRLPQIVLP SVIFIFLYSF 310 320 330 340 350 360 LPHKELRFII YVLPIFCLSA AVFCARMLIN RHKSFFRMIL FFGVILHLLA NVLCTGMFLL 370 380 390 400 410 420 VASKNYPGFD ALNYLQFQNR FDAKKPVTVY IDNACAQTGV NRFLHINDAW TYNKTENLKP 430 440 450 460 470 480 ADLENFDFLV LGTYGNTLKT EVESKFVTWH RPLFFVNSFH QYKIKKSRQF PWIYPEIIYT 490 EKAVVLKNRN YK

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Identification of the hydrophobic glycoproteins of Caenorhabditis elegans." Fan X., She Y.-M., Bagshaw R.D., Callahan J.W., Schachter H., Mahuran D.J. Glycobiology 15:952-964(2005) [PubMed: 15888633] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-413, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U53155 Genomic DNA. Translation: AAC48267.3.
PIR	T28996.
RefSeq	NP_505071.1.
UniGene	Cel.7430
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT22. Glycosyltransferase Family 22.
Genome annotation databases
Ensembl	ZC513.5. Caenorhabditis elegans. [Contig view]
GeneID	179185.
KEGG	cel:ZC513.5.
NMPDR	fig|6239.3.peg.18659.
Organism-specific databases
WormBase	WBGene00022629. ZC513.5.
WormPep	ZC513.5. CE29152. [WorfDB]
Phylogenomic databases
OMA	Q23361. TSPLLWY.
Gene expression databases
ArrayExpress	Q23361.
Family and domain databases
InterPro	IPR005599. Alg9_trans. [Graphical view]
PANTHER	PTHR22760. Alg9_trans. 1 hit.
Pfam	PF03901. Glyco_transf_22. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	904286.

Entry information

Entry name

ALG12_CAEEL

Accession

Primary (citable) accession number: Q23361

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	October 1, 2001
Last modified:	June 16, 2009
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project