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Protein

Serine/threonine-protein kinase mig-15

Gene

mig-15

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in cell migration and signal transduction (PubMed:11967148, PubMed:22732572). Important in several developmental processes including epidermal development, Q neuroblast migrations and muscle arm targeting. Required with ina-1/pat-3 to stabilize the commissural axons growth cone along a precise direction and are required for the cell to respond appropriately when signaling in the growth cone must change (PubMed:11967148). During gonad morphogenesis, involved in distal tip cell (DTC) migration from the dorsal side of the hermaphrodite body to the midbody to allow for formation of gonad arms (PubMed:22732572).2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50ATPPROSITE-ProRule annotation1
Active sitei151Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 35ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • body morphogenesis Source: WormBase
  • dorsal/ventral axon guidance Source: WormBase
  • nematode larval development Source: WormBase
  • neuron projection morphogenesis Source: WormBase
  • regulation of cell migration Source: WormBase
  • regulation of synaptic transmission, GABAergic Source: UniProtKB
  • reproduction Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transducer, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-2559580. Oxidative Stress Induced Senescence.
SignaLinkiQ23356.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase mig-15 (EC:2.7.11.1)
Alternative name(s):
Abnormal cell migration protein 15
Gene namesi
Name:mig-15
ORF Names:ZC504.4
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiZC504.4a; CE25672; WBGene00003247; mig-15.
ZC504.4b; CE25673; WBGene00003247; mig-15.
ZC504.4c; CE28273; WBGene00003247; mig-15.
ZC504.4d; CE32767; WBGene00003247; mig-15.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Mutants exhibit premature branching of commissures (PubMed:11967148). RNAi-mediated knockdown results in gonad distal tip cell (DTC) migration defects whereby DTCs do not migrate to the midbody of the hermaphrodite and as a consequence this leads to abnormal gonadal arm formation during gonad morphogenesis (PubMed:22732572). Double knockdown with mig-38 results in enhanced gonad DTC migration defects (PubMed:22732572).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000863261 – 1096Serine/threonine-protein kinase mig-15Add BLAST1096

Proteomic databases

EPDiQ23356.
PaxDbiQ23356.
PeptideAtlasiQ23356.
PRIDEiQ23356.

Expressioni

Gene expression databases

BgeeiWBGene00003247.

Interactioni

Protein-protein interaction databases

BioGridi46161. 18 interactors.
DIPiDIP-25874N.
IntActiQ23356. 7 interactors.
MINTiMINT-1038041.
STRINGi6239.ZC504.4c.

Structurei

3D structure databases

ProteinModelPortaliQ23356.
SMRiQ23356.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 288Protein kinasePROSITE-ProRule annotationAdd BLAST268
Domaini778 – 1070CNHPROSITE-ProRule annotationAdd BLAST293

Sequence similaritiesi

Contains 1 CNH domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0587. Eukaryota.
ENOG410XPHR. LUCA.
GeneTreeiENSGT00810000125351.
InParanoidiQ23356.
KOiK08840.
OMAiEVIACDQ.
OrthoDBiEOG091G018A.
PhylomeDBiQ23356.

Family and domain databases

InterProiIPR001180. CNH_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00780. CNH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00036. CNH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50219. CNH. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform c (identifier: Q23356-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSSGLDEID LNSLRDPAGI FELIEVVGNG TYGQVYKGRH VKTAQLAAIK
60 70 80 90 100
IMNINEDEED EIKLEINMLK KHSHHRNVAT YYGAFIKKLP SSTGKHDQLW
110 120 130 140 150
LVMEFCGSGS ITDLVKNTKG GSLKEEWIAY ICREILRGLY HLHQSKVIHR
160 170 180 190 200
DIKGQNVLLT DSAEVKLVDF GVSAQLDKTV GRRNTFIGTP YWMAPEVIAC
210 220 230 240 250
DESPEATYDS RSDLWSLGIT ALEMAEGHPP LCDMHPMRAL FLIPRNPPPK
260 270 280 290 300
LKRNKKWTKK FETFIETVLV KDYHQRPYTG ALLRHPFIKE QPHEQTIRHS
310 320 330 340 350
IKEHIDRNRR VKKDDADYEY SGSEDDEPSP NNRGPSMGIR DDSESSSMIP
360 370 380 390 400
MDNTLRKGFQ KLQESSRGFA EPGAQQLRRL PQQPAPAPFQ YQQSRYVEPR
410 420 430 440 450
RESSEVKLRA VSSRGAADGP RHSPASRPRP VSHHQRSPQQ SHPAAPHLAD
460 470 480 490 500
LANYEKRRRS EREERRERER QAHHAMPIAR VSASVPAPQQ SRKMSEPLLI
510 520 530 540 550
THVKPEDLDV LASELSKMGG HHNGRSREES MSPPPPAPPP REASISSITD
560 570 580 590 600
TIDVGELDNG ADAEWDDLKD IMMNGEGTLR GPNKPLPPTP TDGENTLVSD
610 620 630 640 650
VRRNGNGNSG HGAYKGKKIP EIRPGIISLD DDDSDSDNEE GNEPLMFKPI
660 670 680 690 700
NASSSRGALP DLLPKSPQLR RQINDQTRQM SDDRADESAS LFGSFYQPNG
710 720 730 740 750
FQNSDSRSSI QHSFSNRDRE KSFVGYFGGG AGAGGGTVNR PGRPQDINQV
760 770 780 790 800
QVNVTPNSNG TPAENDAPEI RKYKKKFSGE ILCAALWGVN LLIGTDSGLM
810 820 830 840 850
LLDRSGQGKV YPLISRRRFD QMTVLEGQNI LATISGRKRR IRVYYLSWLR
860 870 880 890 900
QKILRTEGAG SANTTEKRNG WVNVGDLQGA IHFKIVRYER IKFLVVGLES
910 920 930 940 950
SIEIYAWAPK PYHKFMSFKS FGSLSHVPLI VDLTVEDNAR LKVLYGSTGG
960 970 980 990 1000
FHAIDLDSAA VYDIYTPAQS GQTTTPHCIV VLPNSNGMQL LLCYDNEGVY
1010 1020 1030 1040 1050
VNTYGRMTKN VVLQWGEMPS SVAYISTGQI MGWGNKAIEI RSVDTGHLDG
1060 1070 1080 1090
VFMHKKAQKL KFLCERNDKV FFSSAKGGGS CQIYFMTLNK PGLTNW
Length:1,096
Mass (Da):122,504
Last modified:June 7, 2005 - v3
Checksum:iC411C11566596386
GO
Isoform a (identifier: Q23356-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     688-696: Missing.

Show »
Length:1,087
Mass (Da):121,544
Checksum:i49FC2C39893F95CD
GO
Isoform b (identifier: Q23356-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     431-435: Missing.
     688-696: Missing.

Note: No experimental confirmation available.
Show »
Length:1,082
Mass (Da):120,956
Checksum:i1648360B61368036
GO
Isoform d (identifier: Q23356-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     356-371: RKGFQKLQESSRGFAE → Q
     688-696: Missing.

Note: No experimental confirmation available.
Show »
Length:1,072
Mass (Da):119,822
Checksum:i1D37093F759D40D0
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014010356 – 371RKGFQ…RGFAE → Q in isoform d. CuratedAdd BLAST16
Alternative sequenceiVSP_014011431 – 435Missing in isoform b. Curated5
Alternative sequenceiVSP_014012688 – 696Missing in isoform a, isoform b and isoform d. 1 Publication9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF087131 mRNA. Translation: AAD14593.1.
AF087132 mRNA. Translation: AAD14594.1.
AF087133 mRNA. Translation: AAD14595.1.
Z50029 Genomic DNA. Translation: CAB63416.1.
Z50029 Genomic DNA. Translation: CAB63417.1.
Z50029 Genomic DNA. Translation: CAC42384.1.
Z50029 Genomic DNA. Translation: CAD57714.1.
PIRiT27622.
T43630.
T43632.
RefSeqiNP_001024971.1. NM_001029800.2. [Q23356-2]
NP_001024972.1. NM_001029801.3. [Q23356-3]
NP_001024973.1. NM_001029802.3. [Q23356-1]
NP_001024974.1. NM_001029803.3. [Q23356-4]
UniGeneiCel.4921.

Genome annotation databases

EnsemblMetazoaiZC504.4c; ZC504.4c; WBGene00003247. [Q23356-1]
GeneIDi181248.
KEGGicel:CELE_ZC504.4.
UCSCiZC504.4c. c. elegans. [Q23356-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF087131 mRNA. Translation: AAD14593.1.
AF087132 mRNA. Translation: AAD14594.1.
AF087133 mRNA. Translation: AAD14595.1.
Z50029 Genomic DNA. Translation: CAB63416.1.
Z50029 Genomic DNA. Translation: CAB63417.1.
Z50029 Genomic DNA. Translation: CAC42384.1.
Z50029 Genomic DNA. Translation: CAD57714.1.
PIRiT27622.
T43630.
T43632.
RefSeqiNP_001024971.1. NM_001029800.2. [Q23356-2]
NP_001024972.1. NM_001029801.3. [Q23356-3]
NP_001024973.1. NM_001029802.3. [Q23356-1]
NP_001024974.1. NM_001029803.3. [Q23356-4]
UniGeneiCel.4921.

3D structure databases

ProteinModelPortaliQ23356.
SMRiQ23356.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi46161. 18 interactors.
DIPiDIP-25874N.
IntActiQ23356. 7 interactors.
MINTiMINT-1038041.
STRINGi6239.ZC504.4c.

Proteomic databases

EPDiQ23356.
PaxDbiQ23356.
PeptideAtlasiQ23356.
PRIDEiQ23356.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiZC504.4c; ZC504.4c; WBGene00003247. [Q23356-1]
GeneIDi181248.
KEGGicel:CELE_ZC504.4.
UCSCiZC504.4c. c. elegans. [Q23356-1]

Organism-specific databases

CTDi181248.
WormBaseiZC504.4a; CE25672; WBGene00003247; mig-15.
ZC504.4b; CE25673; WBGene00003247; mig-15.
ZC504.4c; CE28273; WBGene00003247; mig-15.
ZC504.4d; CE32767; WBGene00003247; mig-15.

Phylogenomic databases

eggNOGiKOG0587. Eukaryota.
ENOG410XPHR. LUCA.
GeneTreeiENSGT00810000125351.
InParanoidiQ23356.
KOiK08840.
OMAiEVIACDQ.
OrthoDBiEOG091G018A.
PhylomeDBiQ23356.

Enzyme and pathway databases

ReactomeiR-CEL-2559580. Oxidative Stress Induced Senescence.
SignaLinkiQ23356.

Miscellaneous databases

PROiQ23356.

Gene expression databases

BgeeiWBGene00003247.

Family and domain databases

InterProiIPR001180. CNH_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00780. CNH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00036. CNH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50219. CNH. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMIG15_CAEEL
AccessioniPrimary (citable) accession number: Q23356
Secondary accession number(s): Q8I4B5
, Q8T8M3, Q95ZI7, Q9UAN7, Q9XYC3, Q9XYC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 7, 2005
Last modified: November 2, 2016
This is version 142 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.