Serine/threonine-protein kinase mig-15

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase mig-15
    EC=2.7.11.1
Alternative name(s):
    Abnormal cell migration protein 15

Gene names

Name:	mig-15
ORF Names:	ZC504.4

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

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Protein attributes

Sequence length	1096 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Involved in cell migration and signal transduction. Important in several developmental processes including epidermal development, Q neuroblast migrations and muscle arm targeting. Required with ina-1/pat-3 to stabilize the commissural axons growth cone along a precise direction and are required for the cell to respond appropriately when signaling in the growth cone must change. Ref.4
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Disruption phenotype	Mutants exhibit premature branching of commissures. Ref.4
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CNH domain. Contains 1 protein kinase domain.

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Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transducer Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell migration Ref.4 Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro signal transduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW small GTPase regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
	Q22174	1	EBI-326940,EBI-311938
	Q22847	1	EBI-326940,EBI-316760
dnj-10	Q8TA83	1	EBI-326940,EBI-331999
pal-1	P34766	1	EBI-326940,EBI-311911
ttx-1	Q9U2Z0	1	EBI-326940,EBI-330989

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Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform c (identifier: Q23356-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform a (identifier: Q23356-2) The sequence of this isoform differs from the canonical sequence as follows: 688-696: Missing.

Isoform b (identifier: Q23356-3) The sequence of this isoform differs from the canonical sequence as follows: 431-435: Missing. 688-696: Missing.
Note: No experimental confirmation available.

Isoform d (identifier: Q23356-4) The sequence of this isoform differs from the canonical sequence as follows: 356-371: RKGFQKLQESSRGFAE → Q 688-696: Missing.
Note: No experimental confirmation available.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1096

1096

Serine/threonine-protein kinase mig-15

PRO_0000086326

Regions

Domain

21 – 288

268

Protein kinase

Domain

773 – 1076

304

CNH

Nucleotide binding

27 – 35

9

ATP By similarity

Sites

Active site

151

1

Proton acceptor By similarity

Binding site

50

1

ATP By similarity

Natural variations

Alternative sequence

356 – 371

16

RKGFQ…RGFAE → Q in isoform d.

VSP_014010

Alternative sequence

431 – 435

5

Missing in isoform b.

VSP_014011

Alternative sequence

688 – 696

9

Missing in isoform a, isoform b and isoform d.

VSP_014012

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Sequences

Sequence		Length	Mass (Da)
Isoform c [UniParc]. Last modified June 7, 2005. Version 3. Checksum: C411C11566596386 Show »	FASTA	1,096	122,504
10 20 30 40 50 60 MSSSGLDEID LNSLRDPAGI FELIEVVGNG TYGQVYKGRH VKTAQLAAIK IMNINEDEED 70 80 90 100 110 120 EIKLEINMLK KHSHHRNVAT YYGAFIKKLP SSTGKHDQLW LVMEFCGSGS ITDLVKNTKG 130 140 150 160 170 180 GSLKEEWIAY ICREILRGLY HLHQSKVIHR DIKGQNVLLT DSAEVKLVDF GVSAQLDKTV 190 200 210 220 230 240 GRRNTFIGTP YWMAPEVIAC DESPEATYDS RSDLWSLGIT ALEMAEGHPP LCDMHPMRAL 250 260 270 280 290 300 FLIPRNPPPK LKRNKKWTKK FETFIETVLV KDYHQRPYTG ALLRHPFIKE QPHEQTIRHS 310 320 330 340 350 360 IKEHIDRNRR VKKDDADYEY SGSEDDEPSP NNRGPSMGIR DDSESSSMIP MDNTLRKGFQ 370 380 390 400 410 420 KLQESSRGFA EPGAQQLRRL PQQPAPAPFQ YQQSRYVEPR RESSEVKLRA VSSRGAADGP 430 440 450 460 470 480 RHSPASRPRP VSHHQRSPQQ SHPAAPHLAD LANYEKRRRS EREERRERER QAHHAMPIAR 490 500 510 520 530 540 VSASVPAPQQ SRKMSEPLLI THVKPEDLDV LASELSKMGG HHNGRSREES MSPPPPAPPP 550 560 570 580 590 600 REASISSITD TIDVGELDNG ADAEWDDLKD IMMNGEGTLR GPNKPLPPTP TDGENTLVSD 610 620 630 640 650 660 VRRNGNGNSG HGAYKGKKIP EIRPGIISLD DDDSDSDNEE GNEPLMFKPI NASSSRGALP 670 680 690 700 710 720 DLLPKSPQLR RQINDQTRQM SDDRADESAS LFGSFYQPNG FQNSDSRSSI QHSFSNRDRE 730 740 750 760 770 780 KSFVGYFGGG AGAGGGTVNR PGRPQDINQV QVNVTPNSNG TPAENDAPEI RKYKKKFSGE 790 800 810 820 830 840 ILCAALWGVN LLIGTDSGLM LLDRSGQGKV YPLISRRRFD QMTVLEGQNI LATISGRKRR 850 860 870 880 890 900 IRVYYLSWLR QKILRTEGAG SANTTEKRNG WVNVGDLQGA IHFKIVRYER IKFLVVGLES 910 920 930 940 950 960 SIEIYAWAPK PYHKFMSFKS FGSLSHVPLI VDLTVEDNAR LKVLYGSTGG FHAIDLDSAA 970 980 990 1000 1010 1020 VYDIYTPAQS GQTTTPHCIV VLPNSNGMQL LLCYDNEGVY VNTYGRMTKN VVLQWGEMPS 1030 1040 1050 1060 1070 1080 SVAYISTGQI MGWGNKAIEI RSVDTGHLDG VFMHKKAQKL KFLCERNDKV FFSSAKGGGS 1090 CQIYFMTLNK PGLTNW « Hide
Isoform a. Checksum: 49FC2C39893F95CD Show »	FASTA	1,087	121,544
10 20 30 40 50 60 MSSSGLDEID LNSLRDPAGI FELIEVVGNG TYGQVYKGRH VKTAQLAAIK IMNINEDEED 70 80 90 100 110 120 EIKLEINMLK KHSHHRNVAT YYGAFIKKLP SSTGKHDQLW LVMEFCGSGS ITDLVKNTKG 130 140 150 160 170 180 GSLKEEWIAY ICREILRGLY HLHQSKVIHR DIKGQNVLLT DSAEVKLVDF GVSAQLDKTV 190 200 210 220 230 240 GRRNTFIGTP YWMAPEVIAC DESPEATYDS RSDLWSLGIT ALEMAEGHPP LCDMHPMRAL 250 260 270 280 290 300 FLIPRNPPPK LKRNKKWTKK FETFIETVLV KDYHQRPYTG ALLRHPFIKE QPHEQTIRHS 310 320 330 340 350 360 IKEHIDRNRR VKKDDADYEY SGSEDDEPSP NNRGPSMGIR DDSESSSMIP MDNTLRKGFQ 370 380 390 400 410 420 KLQESSRGFA EPGAQQLRRL PQQPAPAPFQ YQQSRYVEPR RESSEVKLRA VSSRGAADGP 430 440 450 460 470 480 RHSPASRPRP VSHHQRSPQQ SHPAAPHLAD LANYEKRRRS EREERRERER QAHHAMPIAR 490 500 510 520 530 540 VSASVPAPQQ SRKMSEPLLI THVKPEDLDV LASELSKMGG HHNGRSREES MSPPPPAPPP 550 560 570 580 590 600 REASISSITD TIDVGELDNG ADAEWDDLKD IMMNGEGTLR GPNKPLPPTP TDGENTLVSD 610 620 630 640 650 660 VRRNGNGNSG HGAYKGKKIP EIRPGIISLD DDDSDSDNEE GNEPLMFKPI NASSSRGALP 670 680 690 700 710 720 DLLPKSPQLR RQINDQTRQM SDDRADEQPN GFQNSDSRSS IQHSFSNRDR EKSFVGYFGG 730 740 750 760 770 780 GAGAGGGTVN RPGRPQDINQ VQVNVTPNSN GTPAENDAPE IRKYKKKFSG EILCAALWGV 790 800 810 820 830 840 NLLIGTDSGL MLLDRSGQGK VYPLISRRRF DQMTVLEGQN ILATISGRKR RIRVYYLSWL 850 860 870 880 890 900 RQKILRTEGA GSANTTEKRN GWVNVGDLQG AIHFKIVRYE RIKFLVVGLE SSIEIYAWAP 910 920 930 940 950 960 KPYHKFMSFK SFGSLSHVPL IVDLTVEDNA RLKVLYGSTG GFHAIDLDSA AVYDIYTPAQ 970 980 990 1000 1010 1020 SGQTTTPHCI VVLPNSNGMQ LLLCYDNEGV YVNTYGRMTK NVVLQWGEMP SSVAYISTGQ 1030 1040 1050 1060 1070 1080 IMGWGNKAIE IRSVDTGHLD GVFMHKKAQK LKFLCERNDK VFFSSAKGGG SCQIYFMTLN KPGLTNW « Hide
Isoform b. Checksum: 1648360B61368036 Show »	FASTA	1,082	120,956
10 20 30 40 50 60 MSSSGLDEID LNSLRDPAGI FELIEVVGNG TYGQVYKGRH VKTAQLAAIK IMNINEDEED 70 80 90 100 110 120 EIKLEINMLK KHSHHRNVAT YYGAFIKKLP SSTGKHDQLW LVMEFCGSGS ITDLVKNTKG 130 140 150 160 170 180 GSLKEEWIAY ICREILRGLY HLHQSKVIHR DIKGQNVLLT DSAEVKLVDF GVSAQLDKTV 190 200 210 220 230 240 GRRNTFIGTP YWMAPEVIAC DESPEATYDS RSDLWSLGIT ALEMAEGHPP LCDMHPMRAL 250 260 270 280 290 300 FLIPRNPPPK LKRNKKWTKK FETFIETVLV KDYHQRPYTG ALLRHPFIKE QPHEQTIRHS 310 320 330 340 350 360 IKEHIDRNRR VKKDDADYEY SGSEDDEPSP NNRGPSMGIR DDSESSSMIP MDNTLRKGFQ 370 380 390 400 410 420 KLQESSRGFA EPGAQQLRRL PQQPAPAPFQ YQQSRYVEPR RESSEVKLRA VSSRGAADGP 430 440 450 460 470 480 RHSPASRPRP RSPQQSHPAA PHLADLANYE KRRRSEREER RERERQAHHA MPIARVSASV 490 500 510 520 530 540 PAPQQSRKMS EPLLITHVKP EDLDVLASEL SKMGGHHNGR SREESMSPPP PAPPPREASI 550 560 570 580 590 600 SSITDTIDVG ELDNGADAEW DDLKDIMMNG EGTLRGPNKP LPPTPTDGEN TLVSDVRRNG 610 620 630 640 650 660 NGNSGHGAYK GKKIPEIRPG IISLDDDDSD SDNEEGNEPL MFKPINASSS RGALPDLLPK 670 680 690 700 710 720 SPQLRRQIND QTRQMSDDRA DEQPNGFQNS DSRSSIQHSF SNRDREKSFV GYFGGGAGAG 730 740 750 760 770 780 GGTVNRPGRP QDINQVQVNV TPNSNGTPAE NDAPEIRKYK KKFSGEILCA ALWGVNLLIG 790 800 810 820 830 840 TDSGLMLLDR SGQGKVYPLI SRRRFDQMTV LEGQNILATI SGRKRRIRVY YLSWLRQKIL 850 860 870 880 890 900 RTEGAGSANT TEKRNGWVNV GDLQGAIHFK IVRYERIKFL VVGLESSIEI YAWAPKPYHK 910 920 930 940 950 960 FMSFKSFGSL SHVPLIVDLT VEDNARLKVL YGSTGGFHAI DLDSAAVYDI YTPAQSGQTT 970 980 990 1000 1010 1020 TPHCIVVLPN SNGMQLLLCY DNEGVYVNTY GRMTKNVVLQ WGEMPSSVAY ISTGQIMGWG 1030 1040 1050 1060 1070 1080 NKAIEIRSVD TGHLDGVFMH KKAQKLKFLC ERNDKVFFSS AKGGGSCQIY FMTLNKPGLT NW « Hide
Isoform d. Checksum: 1D37093F759D40D0 Show »	FASTA	1,072	119,822
10 20 30 40 50 60 MSSSGLDEID LNSLRDPAGI FELIEVVGNG TYGQVYKGRH VKTAQLAAIK IMNINEDEED 70 80 90 100 110 120 EIKLEINMLK KHSHHRNVAT YYGAFIKKLP SSTGKHDQLW LVMEFCGSGS ITDLVKNTKG 130 140 150 160 170 180 GSLKEEWIAY ICREILRGLY HLHQSKVIHR DIKGQNVLLT DSAEVKLVDF GVSAQLDKTV 190 200 210 220 230 240 GRRNTFIGTP YWMAPEVIAC DESPEATYDS RSDLWSLGIT ALEMAEGHPP LCDMHPMRAL 250 260 270 280 290 300 FLIPRNPPPK LKRNKKWTKK FETFIETVLV KDYHQRPYTG ALLRHPFIKE QPHEQTIRHS 310 320 330 340 350 360 IKEHIDRNRR VKKDDADYEY SGSEDDEPSP NNRGPSMGIR DDSESSSMIP MDNTLQPGAQ 370 380 390 400 410 420 QLRRLPQQPA PAPFQYQQSR YVEPRRESSE VKLRAVSSRG AADGPRHSPA SRPRPVSHHQ 430 440 450 460 470 480 RSPQQSHPAA PHLADLANYE KRRRSEREER RERERQAHHA MPIARVSASV PAPQQSRKMS 490 500 510 520 530 540 EPLLITHVKP EDLDVLASEL SKMGGHHNGR SREESMSPPP PAPPPREASI SSITDTIDVG 550 560 570 580 590 600 ELDNGADAEW DDLKDIMMNG EGTLRGPNKP LPPTPTDGEN TLVSDVRRNG NGNSGHGAYK 610 620 630 640 650 660 GKKIPEIRPG IISLDDDDSD SDNEEGNEPL MFKPINASSS RGALPDLLPK SPQLRRQIND 670 680 690 700 710 720 QTRQMSDDRA DEQPNGFQNS DSRSSIQHSF SNRDREKSFV GYFGGGAGAG GGTVNRPGRP 730 740 750 760 770 780 QDINQVQVNV TPNSNGTPAE NDAPEIRKYK KKFSGEILCA ALWGVNLLIG TDSGLMLLDR 790 800 810 820 830 840 SGQGKVYPLI SRRRFDQMTV LEGQNILATI SGRKRRIRVY YLSWLRQKIL RTEGAGSANT 850 860 870 880 890 900 TEKRNGWVNV GDLQGAIHFK IVRYERIKFL VVGLESSIEI YAWAPKPYHK FMSFKSFGSL 910 920 930 940 950 960 SHVPLIVDLT VEDNARLKVL YGSTGGFHAI DLDSAAVYDI YTPAQSGQTT TPHCIVVLPN 970 980 990 1000 1010 1020 SNGMQLLLCY DNEGVYVNTY GRMTKNVVLQ WGEMPSSVAY ISTGQIMGWG NKAIEIRSVD 1030 1040 1050 1060 1070 TGHLDGVFMH KKAQKLKFLC ERNDKVFFSS AKGGGSCQIY FMTLNKPGLT NW « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MIG-15, a NIK ortholog of the STE20 family of serine/threonine protein kinases, is involved in cell migration and signal transduction in C. elegans." Zhu X., Acharya P., Hedgecock E.M. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), NUCLEOTIDE SEQUENCE [MRNA] OF 369-1096 (ISOFORM C). Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[3]	"mig-15 encodes a novel Ser/Thr protein kinase of the Ste-20/p65PAK family." Zhu X., Hedgecock E.M. Worm Breeder's Gazette 14(5):76(1997) Cited for: IDENTIFICATION.
[4]	"A conserved interaction between beta1 integrin/PAT-3 and Nck-interacting kinase/MIG-15 that mediates commissural axon navigation in C. elegans." Poinat P., De Arcangelis A., Sookhareea S., Zhu X., Hedgecock E.M., Labouesse M., Georges-Labouesse E. Curr. Biol. 12:622-631(2002) [PubMed: 11967148] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	AF087131 mRNA. Translation: AAD14593.1. AF087132 mRNA. Translation: AAD14594.1. AF087133 mRNA. Translation: AAD14595.1. Z50029 Genomic DNA. Translation: CAB63416.1. Z50029 Genomic DNA. Translation: CAB63417.1. Z50029 Genomic DNA. Translation: CAC42384.1. Z50029 Genomic DNA. Translation: CAD57714.1.
PIR	T27622. T43630. T43632.
RefSeq	NP_001024973.1.
UniGene	Cel.4921
3D structure databases
HSSP	HSSP built from PDB template 1F3M based on UniProtKB Q13153.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q23356. 18 interactions.
Genome annotation databases
Ensembl	ZC504.4. Caenorhabditis elegans. [Contig view]
GeneID	181248.
Organism-specific databases
WormBase	WBGene00003247. mig-15.
WormPep	ZC504.4a. CE25672. [WorfDB] ZC504.4b. CE25673. [WorfDB] ZC504.4c. CE28273. [WorfDB] ZC504.4d. CE32767. [WorfDB]
Phylogenomic databases
OMA	Q23356. EASAMHR.
Enzyme and pathway databases
BRENDA	2.7.11.1. 672.
Gene expression databases
ArrayExpress	Q23356.
Family and domain databases
InterPro	IPR001180. Citron. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00780. CNH. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00036. CNH. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	913088.

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Entry information

Entry name

MIG15_CAEEL

Accession

Primary (citable) accession number: Q23356
Secondary accession number(s): Q8I4B5

Q9XYC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 7, 2005
Last modified:	June 16, 2009
This is version 76 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project

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Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sites

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents