Reviewed,
UniProtKB/Swiss-Prot Q23356 (MIG15_CAEEL)
Last modified
June 16, 2009.
Version 76.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Serine/threonine-protein kinase mig-15 EC=2.7.11.1 Alternative name(s): Abnormal cell migration protein 15 | ||||
| Gene names |
| ||||
| Organism | Caenorhabditis elegans [Complete proteome] | ||||
| Taxonomic identifier | 6239 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Protein attributes
| Sequence length | 1096 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in cell migration and signal transduction. Important in several developmental processes including epidermal development, Q neuroblast migrations and muscle arm targeting. Required with ina-1/pat-3 to stabilize the commissural axons growth cone along a precise direction and are required for the cell to respond appropriately when signaling in the growth cone must change. Ref.4 |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Disruption phenotype | Mutants exhibit premature branching of commissures. Ref.4 |
| Sequence similarities | Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CNH domain. Contains 1 protein kinase domain. |
Ontologies
| Keywords | |
|---|---|
| Coding sequence diversity | Alternative splicing |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Serine/threonine-protein kinase Transducer Transferase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | cell migration Ref.4 Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia developmentInferred from mutant phenotype. Source: WormBase protein amino acid phosphorylationInferred from electronic annotation. Source: InterPro signal transductionInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein bindingInferred from physical interaction. Source: IntAct protein serine/threonine kinase activityInferred from electronic annotation. Source: UniProtKB-KW signal transducer activityInferred from electronic annotation. Source: UniProtKB-KW small GTPase regulator activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| Q22174 | 1 | EBI-326940,EBI-311938 | ||
| Q22847 | 1 | EBI-326940,EBI-316760 | ||
| dnj-10 | Q8TA83 | 1 | EBI-326940,EBI-331999 | |
| pal-1 | P34766 | 1 | EBI-326940,EBI-311911 | |
| ttx-1 | Q9U2Z0 | 1 | EBI-326940,EBI-330989 |
Alternative products
| This entry describes 4 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform c (identifier: Q23356-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform a (identifier: Q23356-2) The sequence of this isoform differs from the canonical sequence as follows: 688-696: Missing. | ||||||
| Isoform b (identifier: Q23356-3) The sequence of this isoform differs from the canonical sequence as follows: 431-435: Missing. 688-696: Missing. | ||||||
| Note: No experimental confirmation available. | ||||||
| Isoform d (identifier: Q23356-4) The sequence of this isoform differs from the canonical sequence as follows: 356-371: RKGFQKLQESSRGFAE → Q 688-696: Missing. | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1096 | 1096 | Serine/threonine-protein kinase mig-15 | PRO_0000086326 | |||||
Regions | |||||||||
| Domain | 21 – 288 | 268 | Protein kinase | ||||||
| Domain | 773 – 1076 | 304 | CNH | ||||||
| Nucleotide binding | 27 – 35 | 9 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 151 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 50 | 1 | ATP By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 356 – 371 | 16 | RKGFQ…RGFAE → Q in isoform d. | VSP_014010 | |||||
| Alternative sequence | 431 – 435 | 5 | Missing in isoform b. | VSP_014011 | |||||
| Alternative sequence | 688 – 696 | 9 | Missing in isoform a, isoform b and isoform d. | VSP_014012 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "MIG-15, a NIK ortholog of the STE20 family of serine/threonine protein kinases, is involved in cell migration and signal transduction in C. elegans." Zhu X., Acharya P., Hedgecock E.M. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), NUCLEOTIDE SEQUENCE [MRNA] OF 369-1096 (ISOFORM C). Strain: Bristol N2. |
| [2] | "Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2. |
| [3] | "mig-15 encodes a novel Ser/Thr protein kinase of the Ste-20/p65PAK family." Zhu X., Hedgecock E.M. Worm Breeder's Gazette 14(5):76(1997) Cited for: IDENTIFICATION. |
| [4] | "A conserved interaction between beta1 integrin/PAT-3 and Nck-interacting kinase/MIG-15 that mediates commissural axon navigation in C. elegans." Poinat P., De Arcangelis A., Sookhareea S., Zhu X., Hedgecock E.M., Labouesse M., Georges-Labouesse E. Curr. Biol. 12:622-631(2002) [PubMed: 11967148] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AF087131 mRNA. Translation: AAD14593.1. AF087132 mRNA. Translation: AAD14594.1. AF087133 mRNA. Translation: AAD14595.1. Z50029 Genomic DNA. Translation: CAB63416.1. Z50029 Genomic DNA. Translation: CAB63417.1. Z50029 Genomic DNA. Translation: CAC42384.1. Z50029 Genomic DNA. Translation: CAD57714.1. | |
| PIR | T27622. T43630. T43632. |
| RefSeq | NP_001024973.1. |
| UniGene | Cel.4921 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1F3M based on UniProtKB Q13153. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q23356. 18 interactions. |
Genome annotation databases | |
| Ensembl | ZC504.4. Caenorhabditis elegans. [Contig view] |
| GeneID | 181248. |
Organism-specific databases | |
| WormBase | WBGene00003247. mig-15. |
| WormPep | ZC504.4a. CE25672. [WorfDB] ZC504.4b. CE25673. [WorfDB] ZC504.4c. CE28273. [WorfDB] ZC504.4d. CE32767. [WorfDB] |
Phylogenomic databases | |
| OMA | Q23356. EASAMHR. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.1. 672. |
Gene expression databases | |
| ArrayExpress | Q23356. |
Family and domain databases | |
| InterPro | IPR001180. Citron. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view] |
| Pfam | PF00780. CNH. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view] |
| ProDom | PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00036. CNH. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view] |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 913088. |
Entry information
| Entry name | MIG15_CAEEL | ||||||||
| Accession | Primary (citable) accession number: Q23356 Secondary accession number(s): Q8I4B5 Q9XYC4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | Caenorhabditis annotation project | ||||||||
Relevant documents
| Caenorhabditis elegans Caenorhabditis elegans: entries, gene names and cross-references to WormPep |
| SIMILARITY comments Index of protein domains and families |

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