Reviewed, UniProtKB/Swiss-Prot Q23316 (NICA_CAEEL)

Names and origin

Protein names

Recommended name:
    Nicastrin
Alternative name(s):
    Anterior-pharynx-defective protein 2

Gene names

Name:	aph-2
ORF Names:	ZC434.6

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	723 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch (glp-1 or lin-12). It may represents a stabilizing cofactor required for the assembly of the gamma-secretase complex. Ref.2 Ref.3
Subunit structure	Component of the gamma-secretase complex, a complex probably composed of the presenilin homodimer (sel-12, hop-1 or spe-4), nicastrin (aph-2), aph-1 and pen-2 Probable.
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Sequence similarities	Belongs to the nicastrin family.

Ontologies

Keywords
Biological process	Notch signaling pathway
Cellular component	Membrane
Domain	Signal Transmembrane
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Notch signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW embryonic development ending in birth or egg hatching Ref.2 Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase protein processing Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 723	707	Nicastrin		PRO_0000019684
Regions
	Topological domain	17 – 678	662	Extracellular Potential
	Transmembrane	679 – 699	21	Potential
	Topological domain	700 – 723	24	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	40	1	N-linked (GlcNAc...) Potential
	Glycosylation	181	1	N-linked (GlcNAc...) Ref.4
	Glycosylation	271	1	N-linked (GlcNAc...) Potential
	Glycosylation	328	1	N-linked (GlcNAc...) Potential
	Glycosylation	409	1	N-linked (GlcNAc...) Potential
	Glycosylation	627	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q23316-1 [UniParc]. Last modified September 26, 2003. Version 3. Checksum: C7DF2D12ADBF8D9D	FASTA	723	81,659
	10 20 30 40 50 60 MKKWLVIVLI IAGIRCDGFS DQVFRTLFIG EGNACYRTFN KTHEFGCQAN RENENGLIVR 70 80 90 100 110 120 IDKQEDFKNL DSCWNSFYPK YSGKYWALLP VNLIRRDTIS QLKSSKCLSG IVLYNSGESI 130 140 150 160 170 180 HPGDESTAAS HDAECPNAAS DYYLQDKNEE YCERKINSRG AITRDGLMKI DWRIQMVFID 190 200 210 220 230 240 NSTDLEIIEK CYSMFNKPKE DGSSGYPYCG MSFRLANMAA GNSEICYRRG KNDAKLFQMN 250 260 270 280 290 300 IDSGDAPQLC GAMHSDNIFA FPTPIPTSPT NETIITSKYM MVTARMDSFG MIPEISVGEV 310 320 330 340 350 360 SVLTSIISVL AAARSMGTQI EKWQKASNTS NRNVFFAFFN GESLDYIGSG AAAYQMENGK 370 380 390 400 410 420 FPQMIRSDRT HIHPIRPNEL DYILEVQQIG VAKGRKYYVH VDGERYQQNK TQTDRVIDRI 430 440 450 460 470 480 ERGLRSHAFD LEKPSGSGDR VPPASWHSFA KADAHVQSVL LAPYGKEYEY QRVNSILDKN 490 500 510 520 530 540 EWTEDEREKA IQEIEAVSTA ILAAAADYVG VETDEVVAKV DKKLITTIFD CLITSNFWFD 550 560 570 580 590 600 CDFMQKLDGG RYHKLFNSYG FNQKSTYISM ESHTAFPTVL HWLTIFALGS DKETLNVKSE 610 620 630 640 650 660 KSCSHLGQFQ AFQMYTYTWQ PNPYTGNFSC LKSAIVKKVM VSPAVDSQTP EEEMNTRYST 670 680 690 700 710 720 WMESVYIIES VNLYLMEDAS FEYTMILIAV ISALLSIFAV GRCSETTFIV DEGEPAAEGG EPL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Nicastrin modulates presenilin-mediated notch/glp-1 signal transduction and betaAPP processing." Yu G., Nishimura M., Arawaka S., Levitan D., Zhang L., Tandon A., Song Y.-Q., Rogaeva E., Chen F., Kawarai T., Supala A., Levesque L., Yu H., Yang D.-S., Holmes E., Milman P., Liang Y., Zhang D.M. , Xu D.H., Sato C., Rogaev E., Smith M., Janus C., Zhang Y., Aebersold R., Farrer L.S., Sorbi S., Bruni A., Fraser P.E., St George-Hyslop P.H. Nature 407:48-54(2000) [PubMed: 10993067] [Abstract] Cited for: FUNCTION, GENE NAME.
[3]	"aph-2 encodes a novel extracellular protein required for GLP-1-mediated signaling." Goutte C., Hepler W., Mickey K.M., Priess J.R. Development 127:2481-2492(2000) [PubMed: 10804188] [Abstract] Cited for: FUNCTION.
[4]	"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins." Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T. Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed: 17761667] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-181, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z75714 Genomic DNA. Translation: CAB00063.2.
PIR	T27570.
RefSeq	NP_492712.2.
UniGene	Cel.5001
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q23316.
Genome annotation databases
Ensembl	ZC434.6b; ZC434.6b; ZC434.6; Caenorhabditis elegans. [Genome view]
GeneID	172905.
NMPDR	fig|6239.3.peg.2761.
UCSC	ZC434.6b. c. elegans.
Organism-specific databases
CTD	172905.
WormBase	WBGene00000148. aph-2.
WormPep	ZC434.6. CE34446. [WorfDB]
Phylogenomic databases
OMA	AGNSEIC
Gene expression databases
ArrayExpress	Q23316.
Family and domain databases
InterPro	IPR008710. Nicastrin. [Graphical view]
PANTHER	PTHR21092. Nicastrin. 1 hit.
Pfam	PF05450. Nicastrin. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	877471.

Entry information

Entry name

NICA_CAEEL

Accession

Primary (citable) accession number: Q23316

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	September 26, 2003
Last modified:	November 3, 2009
This is version 66 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project