Q23316 (NICA_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nicastrin

Alternative name(s):
Anterior-pharynx-defective protein 2

Gene names

Name:	aph-2
ORF Names:	ZC434.6

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	723 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch (glp-1 or lin-12). It may represents a stabilizing cofactor required for the assembly of the gamma-secretase complex. Ref.2 Ref.3
Subunit structure	Component of the gamma-secretase complex, a complex probably composed of the presenilin homodimer (sel-12, hop-1 or spe-4), nicastrin (aph-2), aph-1 and pen-2 Probable.
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Sequence similarities	Belongs to the nicastrin family.

Ontologies

Keywords
Biological process	Notch signaling pathway
Cellular component	Membrane
Domain	Signal Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Notch signaling pathway Inferred from mutant phenotype Ref.3. Source: WormBase cell fate commitment involved in pattern specification Inferred from mutant phenotype Ref.3. Source: WormBase embryo development ending in birth or egg hatching Inferred from mutant phenotype Ref.2. Source: WormBase embryonic morphogenesis Inferred from mutant phenotype Ref.3. Source: WormBase oviposition Inferred from mutant phenotype Ref.3. Source: WormBase pharynx development Inferred from mutant phenotype Ref.3. Source: WormBase protein processing Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay Ref.3. Source: WormBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 723	707	Nicastrin		PRO_0000019684
Regions
	Topological domain	17 – 678	662	Extracellular Potential
	Transmembrane	679 – 699	21	Helical; Potential
	Topological domain	700 – 723	24	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	40	1	N-linked (GlcNAc...) Potential
	Glycosylation	181	1	N-linked (GlcNAc...) Ref.4
	Glycosylation	271	1	N-linked (GlcNAc...) Potential
	Glycosylation	328	1	N-linked (GlcNAc...) Potential
	Glycosylation	409	1	N-linked (GlcNAc...) Potential
	Glycosylation	627	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q23316 [UniParc]. Last modified September 26, 2003. Version 3. Checksum: C7DF2D12ADBF8D9D	FASTA	723	81,659
	10 20 30 40 50 60 MKKWLVIVLI IAGIRCDGFS DQVFRTLFIG EGNACYRTFN KTHEFGCQAN RENENGLIVR 70 80 90 100 110 120 IDKQEDFKNL DSCWNSFYPK YSGKYWALLP VNLIRRDTIS QLKSSKCLSG IVLYNSGESI 130 140 150 160 170 180 HPGDESTAAS HDAECPNAAS DYYLQDKNEE YCERKINSRG AITRDGLMKI DWRIQMVFID 190 200 210 220 230 240 NSTDLEIIEK CYSMFNKPKE DGSSGYPYCG MSFRLANMAA GNSEICYRRG KNDAKLFQMN 250 260 270 280 290 300 IDSGDAPQLC GAMHSDNIFA FPTPIPTSPT NETIITSKYM MVTARMDSFG MIPEISVGEV 310 320 330 340 350 360 SVLTSIISVL AAARSMGTQI EKWQKASNTS NRNVFFAFFN GESLDYIGSG AAAYQMENGK 370 380 390 400 410 420 FPQMIRSDRT HIHPIRPNEL DYILEVQQIG VAKGRKYYVH VDGERYQQNK TQTDRVIDRI 430 440 450 460 470 480 ERGLRSHAFD LEKPSGSGDR VPPASWHSFA KADAHVQSVL LAPYGKEYEY QRVNSILDKN 490 500 510 520 530 540 EWTEDEREKA IQEIEAVSTA ILAAAADYVG VETDEVVAKV DKKLITTIFD CLITSNFWFD 550 560 570 580 590 600 CDFMQKLDGG RYHKLFNSYG FNQKSTYISM ESHTAFPTVL HWLTIFALGS DKETLNVKSE 610 620 630 640 650 660 KSCSHLGQFQ AFQMYTYTWQ PNPYTGNFSC LKSAIVKKVM VSPAVDSQTP EEEMNTRYST 670 680 690 700 710 720 WMESVYIIES VNLYLMEDAS FEYTMILIAV ISALLSIFAV GRCSETTFIV DEGEPAAEGG EPL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Nicastrin modulates presenilin-mediated notch/glp-1 signal transduction and betaAPP processing." Yu G., Nishimura M., Arawaka S., Levitan D., Zhang L., Tandon A., Song Y.-Q., Rogaeva E., Chen F., Kawarai T., Supala A., Levesque L., Yu H., Yang D.-S., Holmes E., Milman P., Liang Y., Zhang D.M. , Xu D.H., Sato C., Rogaev E., Smith M., Janus C., Zhang Y., Aebersold R., Farrer L.S., Sorbi S., Bruni A., Fraser P.E., St George-Hyslop P.H. Nature 407:48-54(2000) [PubMed: 10993067] [Abstract] Cited for: FUNCTION, GENE NAME.
[3]	"aph-2 encodes a novel extracellular protein required for GLP-1-mediated signaling." Goutte C., Hepler W., Mickey K.M., Priess J.R. Development 127:2481-2492(2000) [PubMed: 10804188] [Abstract] Cited for: FUNCTION.
[4]	"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins." Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T. Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed: 17761667] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-181, MASS SPECTROMETRY. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z75714 Genomic DNA. Translation: CAB00063.2.
PIR	T27570.
RefSeq	NP_492712.2. NM_060311.2.
UniGene	Cel.5001.
3D structure databases
ProteinModelPortal	Q23316.
ModBase	Search...
Protein-protein interaction databases
STRING	Q23316.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	ZC434.6b; ZC434.6b; ZC434.6.
GeneID	172905.
KEGG	cel:ZC434.6.
NMPDR	fig|6239.3.peg.2761.
UCSC	ZC434.6b. c. elegans.
Organism-specific databases
CTD	5657004.
WormBase	ZC434.6b; CE34446; WBGene00000148; aph-2.
Phylogenomic databases
eggNOG	meNOG06166.
GeneTree	EMGT00050000004681.
HOGENOM	HBG377880.
InParanoid	Q23316.
OMA	AGNSEIC.
PhylomeDB	Q23316.
Gene expression databases
ArrayExpress	Q23316.
Family and domain databases
InterPro	IPR008710. Nicastrin. [Graphical view]
KO	K06171.
PANTHER	PTHR21092. Nicastrin. 1 hit.
Pfam	PF05450. Nicastrin. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	877471.

Entry information

Entry name

NICA_CAEEL

Accession

Primary (citable) accession number: Q23316

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	September 26, 2003
Last modified:	November 16, 2011
This is version 81 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project