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Protein

tRNA (guanine(26)-N(2))-dimethyltransferase

Gene

trm-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Dimethylates a single guanine residue at position 26 of most tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.

Catalytic activityi

2 S-adenosyl-L-methionine + guanine(26) in tRNA = 2 S-adenosyl-L-homocysteine + N(2)-dimethylguanine(26) in tRNA.PROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

BRENDAi2.1.1.216. 1045.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine(26)-N(2))-dimethyltransferase (EC:2.1.1.216)
Alternative name(s):
tRNA 2,2-dimethylguanosine-26 methyltransferase
tRNA(guanine-26,N(2)-N(2)) methyltransferase
tRNA(m(2,2)G26)dimethyltransferase
Gene namesi
Name:trm-1
Synonyms:trm1
ORF Names:ZC376.5
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiZC376.5; CE15201; WBGene00006613; trm-1.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi246 – 2461R → G: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 526526tRNA (guanine(26)-N(2))-dimethyltransferasePRO_0000147673Add
BLAST

Proteomic databases

EPDiQ23270.
PaxDbiQ23270.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi6239.ZC376.5.1.

Structurei

3D structure databases

ProteinModelPortaliQ23270.
SMRiQ23270. Positions 24-437.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 441420Trm1 methyltransferasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.PROSITE-ProRule annotation
Contains 1 Trm1 methyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1253. Eukaryota.
COG1867. LUCA.
GeneTreeiENSGT00530000063646.
HOGENOMiHOG000177995.
InParanoidiQ23270.
KOiK00555.
OMAiNPEMELN.
PhylomeDBiQ23270.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR002905. Trm1.
[Graphical view]
PANTHERiPTHR10631. PTHR10631. 1 hit.
PfamiPF02005. TRM. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 3 hits.
TIGRFAMsiTIGR00308. TRM1. 1 hit.
PROSITEiPS51626. SAM_MT_TRM1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q23270-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTENVNSSGD SAIKSEDKEE VTVIQEGQAK VGFHGPVFYN PVQEFNRDLT
60 70 80 90 100
VTVLRQFSAD HQKWAEEQKQ LKTEEEPPKK KNKLAINEDG KIRILDALSA
110 120 130 140 150
SGLRALRFSK EVPNVGFIMA NDFSDNAVAS IQENVKLNGV EDIVEAHFGD
160 170 180 190 200
AVMTMMEHRG IDKRFHAVDL DPYGTASTFL DSAVQCVADR GILMVTCTDM
210 220 230 240 250
AVLCGNTPEA CYNKYDAVTT RMKCCHEVGL RILLRAIDSA ANRYTRYIEP
260 270 280 290 300
LVSISVDFYV RVFVRVHTGA FQAKQSGTKV GTVLVCSGCH SMEPLVLLKR
310 320 330 340 350
GEGNQQSKYS IPTVRHSISG PGNRCIHCLL PLHQIGPIYL APIHSKPFVT
360 370 380 390 400
SLLERLKSTP EAERLGTHGR LQGVLTMVNE ELDDVLYYEH NQMANVVKVS
410 420 430 440 450
VPKSQSVRSA ILNAGFKVSG SHCNPRAIKT NAPMHLLWDI YRQVAKDTSV
460 470 480 490 500
DREKRLAKES AGYHILGQPI TNTVNFTLHP GAIEQAKKEN LVRFQCNKGK
510 520
NWGPRQKAKG SVNSTKAGFQ LTEHKE
Length:526
Mass (Da):58,400
Last modified:November 1, 1996 - v1
Checksum:i5CF69A2BA917D3A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059608 mRNA. Translation: AAD13737.1.
Z77136 Genomic DNA. Translation: CAB00881.1.
PIRiT43053.
RefSeqiNP_506513.1. NM_074112.5.
UniGeneiCel.2073.

Genome annotation databases

EnsemblMetazoaiZC376.5.1; ZC376.5.1; WBGene00006613.
ZC376.5.2; ZC376.5.2; WBGene00006613.
GeneIDi179920.
KEGGicel:CELE_ZC376.5.
UCSCiZC376.5. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059608 mRNA. Translation: AAD13737.1.
Z77136 Genomic DNA. Translation: CAB00881.1.
PIRiT43053.
RefSeqiNP_506513.1. NM_074112.5.
UniGeneiCel.2073.

3D structure databases

ProteinModelPortaliQ23270.
SMRiQ23270. Positions 24-437.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.ZC376.5.1.

Proteomic databases

EPDiQ23270.
PaxDbiQ23270.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiZC376.5.1; ZC376.5.1; WBGene00006613.
ZC376.5.2; ZC376.5.2; WBGene00006613.
GeneIDi179920.
KEGGicel:CELE_ZC376.5.
UCSCiZC376.5. c. elegans.

Organism-specific databases

CTDi179920.
WormBaseiZC376.5; CE15201; WBGene00006613; trm-1.

Phylogenomic databases

eggNOGiKOG1253. Eukaryota.
COG1867. LUCA.
GeneTreeiENSGT00530000063646.
HOGENOMiHOG000177995.
InParanoidiQ23270.
KOiK00555.
OMAiNPEMELN.
PhylomeDBiQ23270.

Enzyme and pathway databases

BRENDAi2.1.1.216. 1045.

Miscellaneous databases

PROiQ23270.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR002905. Trm1.
[Graphical view]
PANTHERiPTHR10631. PTHR10631. 1 hit.
PfamiPF02005. TRM. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 3 hits.
TIGRFAMsiTIGR00308. TRM1. 1 hit.
PROSITEiPS51626. SAM_MT_TRM1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Caenorhabditis elegans ZC376.5 encodes a tRNA (m2/2G(26))dimethyltransferance in which (246)arginine is important for the enzyme activity."
    Liu J.M., Zhou G.Q., Straby K.B.
    Gene 226:73-81(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, MUTAGENESIS OF ARG-246.
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiTRM1_CAEEL
AccessioniPrimary (citable) accession number: Q23270
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.