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Q23126 (TRMB_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
ORF Names:W02B12.10
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length256 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA (guanine-N7)-methylation

Inferred from sequence or structural similarity. Source: GOC

tRNA modification

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 256256tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000171433

Regions

Region97 – 982S-adenosyl-L-methionine binding By similarity
Region132 – 1332S-adenosyl-L-methionine binding By similarity
Region230 – 2323S-adenosyl-L-methionine binding By similarity

Sites

Active site1551 By similarity
Binding site741S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1521S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q23126 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 4F4FAF58CB25FD24

FASTA25630,041
        10         20         30         40         50         60 
MDITPALTEM ELDHKPTCET VPGLPQKKHY RQRAHSNPHS DHDIEYPLTP NHMDWTKYYG 

        70         80         90        100        110        120 
DYTKGRQVDF ADIGCGYGGL LMRLSPKYPD NLMIGMEIRV KVSDYVNEKI QALRKHHAEA 

       130        140        150        160        170        180 
GHYRNVAVLR SNAMKYMPNY FHKGQLSKMF FLFPDPHFKN KKHKWRIITP TLLSEYAYVL 

       190        200        210        220        230        240 
REGGIIYTIT DVKDLHEWMV KHLSEHPLFE RLTEEEMKKD PIVEMLFEST EEGQKVTRND 

       250 
GGKWPAIFRR LPNPAL 

« Hide

References

[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z66521 Genomic DNA. Translation: CAA91400.1.
PIRT26090.
RefSeqNP_496448.1. NM_064047.3.
UniGeneCel.14230.

3D structure databases

ProteinModelPortalQ23126.
SMRQ23126. Positions 46-256.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING6239.W02B12.10.

Proteomic databases

PaxDbQ23126.
PRIDEQ23126.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaW02B12.10; W02B12.10; W02B12.10.
GeneID174753.
KEGGcel:CELE_W02B12.10.
UCSCW02B12.10. c. elegans.

Organism-specific databases

CTD174753.
WormBaseW02B12.10; CE03770; WBGene00012205.

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000260965.
InParanoidQ23126.
KOK03439.
OMARAHSNPI.
OrthoDBEOG7PS1GC.
PhylomeDBQ23126.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio885350.
PROQ23126.

Entry information

Entry nameTRMB_CAEEL
AccessionPrimary (citable) accession number: Q23126
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase