Q23126 (TRMB_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

ORF Names:

W02B12.10

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA.
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine tRNA-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from sequence or structural similarity. Source: GOC tRNA modification Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	tRNA (guanine-N(7)-)-methyltransferase		PRO_0000171433
Regions
	Region	97 – 98	2	S-adenosyl-L-methionine binding By similarity
	Region	132 – 133	2	S-adenosyl-L-methionine binding By similarity
	Region	230 – 232	3	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	155	1	By similarity
	Binding site	74	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	152	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q23126 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4F4FAF58CB25FD24	FASTA	256	30,041
	10 20 30 40 50 60 MDITPALTEM ELDHKPTCET VPGLPQKKHY RQRAHSNPHS DHDIEYPLTP NHMDWTKYYG 70 80 90 100 110 120 DYTKGRQVDF ADIGCGYGGL LMRLSPKYPD NLMIGMEIRV KVSDYVNEKI QALRKHHAEA 130 140 150 160 170 180 GHYRNVAVLR SNAMKYMPNY FHKGQLSKMF FLFPDPHFKN KKHKWRIITP TLLSEYAYVL 190 200 210 220 230 240 REGGIIYTIT DVKDLHEWMV KHLSEHPLFE RLTEEEMKKD PIVEMLFEST EEGQKVTRND 250 GGKWPAIFRR LPNPAL

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z66521 Genomic DNA. Translation: CAA91400.1.
PIR	T26090.
RefSeq	NP_496448.1. NM_064047.3.
3D structure databases
ProteinModelPortal	Q23126.
SMR	Q23126. Positions 37-256.
ModBase	Search...
Protein-protein interaction databases
STRING	6239.W02B12.10.
Proteomic databases
PaxDb	Q23126.
PRIDE	Q23126.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	W02B12.10; W02B12.10; W02B12.10.
GeneID	174753.
KEGG	cel:CELE_W02B12.10.
UCSC	W02B12.10. c. elegans.
Organism-specific databases
CTD	174753.
WormBase	W02B12.10; CE03770; WBGene00012205.
Phylogenomic databases
eggNOG	COG0220.
GeneTree	ENSGT00390000017840.
HOGENOM	HOG000260965.
InParanoid	Q23126.
KO	K03439.
OMA	RAHSNPI.
Enzyme and pathway databases
UniPathway	UPA00989.
Family and domain databases
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...
Other
NextBio	885350.

Entry information

Entry name

TRMB_CAEEL

Accession

Primary (citable) accession number: Q23126

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project