Reviewed, UniProtKB/Swiss-Prot Q23126 (TRMB_CAEEL)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.33
Alternative name(s):
    tRNA(m7G46)-methyltransferase

Gene names

ORF Names:

W02B12.10

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(7)-methylguanine.
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine tRNA-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase tRNA modification Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	tRNA (guanine-N(7)-)-methyltransferase		PRO_0000171433
Regions
	Region	97 – 98	2	S-adenosyl-L-methionine binding By similarity
	Region	132 – 133	2	S-adenosyl-L-methionine binding By similarity
	Region	230 – 232	3	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	155	1	By similarity
	Binding site	74	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	152	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q23126-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4F4FAF58CB25FD24	FASTA	256	30,041
	10 20 30 40 50 60 MDITPALTEM ELDHKPTCET VPGLPQKKHY RQRAHSNPHS DHDIEYPLTP NHMDWTKYYG 70 80 90 100 110 120 DYTKGRQVDF ADIGCGYGGL LMRLSPKYPD NLMIGMEIRV KVSDYVNEKI QALRKHHAEA 130 140 150 160 170 180 GHYRNVAVLR SNAMKYMPNY FHKGQLSKMF FLFPDPHFKN KKHKWRIITP TLLSEYAYVL 190 200 210 220 230 240 REGGIIYTIT DVKDLHEWMV KHLSEHPLFE RLTEEEMKKD PIVEMLFEST EEGQKVTRND 250 GGKWPAIFRR LPNPAL

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z66521 Genomic DNA. Translation: CAA91400.1.
PIR	T26090.
RefSeq	NP_496448.1.
UniGene	Cel.14230
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	W02B12.10. Caenorhabditis elegans. [Contig view]
GeneID	174753.
KEGG	cel:W02B12.10.
NMPDR	fig|6239.3.peg.7486.
Organism-specific databases
WormBase	WBGene00012205. W02B12.10.
WormPep	W02B12.10. CE03770. [WorfDB]
Phylogenomic databases
OMA	Q23126. PDPHFKQ.
Enzyme and pathway databases
BRENDA	2.1.1.33. 672.
Gene expression databases
ArrayExpress	Q23126.
Family and domain databases
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
PANTHER	PTHR23417:SF1. Methyltransf_4. 1 hit.
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. CHP91. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	885350.

Entry information

Entry name

TRMB_CAEEL

Accession

Primary (citable) accession number: Q23126

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project