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Q23126

- TRMB_CAEEL

UniProt

Q23126 - TRMB_CAEEL

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

W02B12.10

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei74 – 741S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Binding sitei152 – 1521S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Active sitei155 – 1551UniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
    2. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. RNA (guanine-N7)-methylation Source: GOC
    2. tRNA modification Source: UniProtKB

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine, tRNA-binding

    Enzyme and pathway databases

    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    ORF Names:W02B12.10
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome II

    Organism-specific databases

    WormBaseiW02B12.10; CE03770; WBGene00012205.

    Subcellular locationi

    Nucleus UniRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 256256tRNA (guanine-N(7)-)-methyltransferasePRO_0000171433Add
    BLAST

    Proteomic databases

    PaxDbiQ23126.
    PRIDEiQ23126.

    Interactioni

    Protein-protein interaction databases

    STRINGi6239.W02B12.10.

    Structurei

    3D structure databases

    ProteinModelPortaliQ23126.
    SMRiQ23126. Positions 37-256.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni97 – 982S-adenosyl-L-methionine bindingUniRule annotation
    Regioni132 – 1332S-adenosyl-L-methionine bindingUniRule annotation
    Regioni230 – 2323S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    GeneTreeiENSGT00390000017840.
    HOGENOMiHOG000260965.
    InParanoidiQ23126.
    KOiK03439.
    OMAiRAHSNPI.
    OrthoDBiEOG7PS1GC.
    PhylomeDBiQ23126.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q23126-1 [UniParc]FASTAAdd to Basket

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    MDITPALTEM ELDHKPTCET VPGLPQKKHY RQRAHSNPHS DHDIEYPLTP    50
    NHMDWTKYYG DYTKGRQVDF ADIGCGYGGL LMRLSPKYPD NLMIGMEIRV 100
    KVSDYVNEKI QALRKHHAEA GHYRNVAVLR SNAMKYMPNY FHKGQLSKMF 150
    FLFPDPHFKN KKHKWRIITP TLLSEYAYVL REGGIIYTIT DVKDLHEWMV 200
    KHLSEHPLFE RLTEEEMKKD PIVEMLFEST EEGQKVTRND GGKWPAIFRR 250
    LPNPAL 256
    Length:256
    Mass (Da):30,041
    Last modified:November 1, 1996 - v1
    Checksum:i4F4FAF58CB25FD24
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z66521 Genomic DNA. Translation: CAA91400.1.
    PIRiT26090.
    RefSeqiNP_496448.1. NM_064047.3.
    UniGeneiCel.14230.

    Genome annotation databases

    EnsemblMetazoaiW02B12.10; W02B12.10; WBGene00012205.
    GeneIDi174753.
    KEGGicel:CELE_W02B12.10.
    UCSCiW02B12.10. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z66521 Genomic DNA. Translation: CAA91400.1 .
    PIRi T26090.
    RefSeqi NP_496448.1. NM_064047.3.
    UniGenei Cel.14230.

    3D structure databases

    ProteinModelPortali Q23126.
    SMRi Q23126. Positions 37-256.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 6239.W02B12.10.

    Proteomic databases

    PaxDbi Q23126.
    PRIDEi Q23126.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai W02B12.10 ; W02B12.10 ; WBGene00012205 .
    GeneIDi 174753.
    KEGGi cel:CELE_W02B12.10.
    UCSCi W02B12.10. c. elegans.

    Organism-specific databases

    CTDi 174753.
    WormBasei W02B12.10 ; CE03770 ; WBGene00012205 .

    Phylogenomic databases

    eggNOGi COG0220.
    GeneTreei ENSGT00390000017840.
    HOGENOMi HOG000260965.
    InParanoidi Q23126.
    KOi K03439.
    OMAi RAHSNPI.
    OrthoDBi EOG7PS1GC.
    PhylomeDBi Q23126.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .

    Miscellaneous databases

    NextBioi 885350.
    PROi Q23126.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.

    Entry informationi

    Entry nameiTRMB_CAEEL
    AccessioniPrimary (citable) accession number: Q23126
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2003
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3