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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

W02B12.10

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei74 – 741S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei152 – 1521S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Active sitei155 – 1551UniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: GO_Central
  2. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA (guanine-N7)-methylation Source: GO_Central
  2. tRNA methylation Source: GO_Central
  3. tRNA modification Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
ORF Names:W02B12.10
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome II

Organism-specific databases

WormBaseiW02B12.10; CE03770; WBGene00012205.

Subcellular locationi

Nucleus UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
  2. tRNA methyltransferase complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 256256tRNA (guanine-N(7)-)-methyltransferasePRO_0000171433Add
BLAST

Proteomic databases

PaxDbiQ23126.
PRIDEiQ23126.

Interactioni

Protein-protein interaction databases

STRINGi6239.W02B12.10.

Structurei

3D structure databases

ProteinModelPortaliQ23126.
SMRiQ23126. Positions 37-256.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni97 – 982S-adenosyl-L-methionine bindingUniRule annotation
Regioni132 – 1332S-adenosyl-L-methionine bindingUniRule annotation
Regioni230 – 2323S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
GeneTreeiENSGT00390000017840.
HOGENOMiHOG000260965.
InParanoidiQ23126.
KOiK03439.
OMAiMCTHFEE.
OrthoDBiEOG7PS1GC.
PhylomeDBiQ23126.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q23126-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDITPALTEM ELDHKPTCET VPGLPQKKHY RQRAHSNPHS DHDIEYPLTP
60 70 80 90 100
NHMDWTKYYG DYTKGRQVDF ADIGCGYGGL LMRLSPKYPD NLMIGMEIRV
110 120 130 140 150
KVSDYVNEKI QALRKHHAEA GHYRNVAVLR SNAMKYMPNY FHKGQLSKMF
160 170 180 190 200
FLFPDPHFKN KKHKWRIITP TLLSEYAYVL REGGIIYTIT DVKDLHEWMV
210 220 230 240 250
KHLSEHPLFE RLTEEEMKKD PIVEMLFEST EEGQKVTRND GGKWPAIFRR

LPNPAL
Length:256
Mass (Da):30,041
Last modified:November 1, 1996 - v1
Checksum:i4F4FAF58CB25FD24
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z66521 Genomic DNA. Translation: CAA91400.1.
PIRiT26090.
RefSeqiNP_496448.1. NM_064047.3.
UniGeneiCel.14230.

Genome annotation databases

EnsemblMetazoaiW02B12.10; W02B12.10; WBGene00012205.
GeneIDi174753.
KEGGicel:CELE_W02B12.10.
UCSCiW02B12.10. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z66521 Genomic DNA. Translation: CAA91400.1.
PIRiT26090.
RefSeqiNP_496448.1. NM_064047.3.
UniGeneiCel.14230.

3D structure databases

ProteinModelPortaliQ23126.
SMRiQ23126. Positions 37-256.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.W02B12.10.

Proteomic databases

PaxDbiQ23126.
PRIDEiQ23126.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiW02B12.10; W02B12.10; WBGene00012205.
GeneIDi174753.
KEGGicel:CELE_W02B12.10.
UCSCiW02B12.10. c. elegans.

Organism-specific databases

CTDi174753.
WormBaseiW02B12.10; CE03770; WBGene00012205.

Phylogenomic databases

eggNOGiCOG0220.
GeneTreeiENSGT00390000017840.
HOGENOMiHOG000260965.
InParanoidiQ23126.
KOiK03439.
OMAiMCTHFEE.
OrthoDBiEOG7PS1GC.
PhylomeDBiQ23126.

Enzyme and pathway databases

UniPathwayiUPA00989.

Miscellaneous databases

NextBioi885350.
PROiQ23126.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiTRMB_CAEEL
AccessioniPrimary (citable) accession number: Q23126
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: November 1, 1996
Last modified: February 4, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.