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Protein

Serine/threonine-protein kinase unc-51

Gene

unc-51

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein kinase important for axonal elongation and axonal guidance (PubMed:7958904, PubMed:15539493). Functions in the CAN axons to direct both anterior and posterior migrations (PubMed:15539493). Phosphorylates both unc-14 and vab-8 (PubMed:15539493). Plays a role in autophagy (PubMed:12958363, PubMed:17890369). Interaction with autophagy related proteins such as atg-13 links it to the autophagy machinery to in turn promote P-granule degradation in somatic cells (PubMed:19377305). Regulates cell size (PubMed:17890369). Plays a role in male tail ray pattern formation (PubMed:17890369). May be required for normal dauer morphogenesis (PubMed:12958363).5 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Mg2+1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39ATPCurated1
Active sitei134Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 23ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • protein kinase activity Source: WormBase
  • protein serine/threonine kinase activity Source: WormBase

GO - Biological processi

  • apoptotic cell clearance Source: WormBase
  • autophagy Source: WormBase
  • axon guidance Source: WormBase
  • axonogenesis Source: WormBase
  • cell migration Source: WormBase
  • dauer larval development Source: WormBase
  • determination of adult lifespan Source: WormBase
  • embryo development ending in birth or egg hatching Source: WormBase
  • nematode male tail tip morphogenesis Source: WormBase
  • programmed cell death Source: WormBase
  • protein autophosphorylation Source: WormBase
  • protein phosphorylation Source: WormBase
  • regulation of axon extension Source: WormBase
  • regulation of cell size Source: WormBase
  • regulation of multicellular organism growth Source: WormBase
  • regulation of protein localization Source: WormBase

Keywordsi

Molecular functionDevelopmental protein, Kinase, Serine/threonine-protein kinase, Transferase
Biological processDifferentiation, Neurogenesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 1045.
ReactomeiR-CEL-1632852. Macroautophagy.
R-CEL-8854214. TBC/RABGAPs.
SignaLinkiQ23023.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase unc-51 (EC:2.7.11.11 Publication)
Alternative name(s):
Uncoordinated protein 51
Gene namesi
Name:unc-51Imported
ORF Names:Y60A3A.1Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiY60A3A.1; CE24516; WBGene00006786; unc-51.

Subcellular locationi

GO - Cellular componenti

  • axon Source: WormBase
  • neuronal cell body Source: WormBase
  • neuron projection Source: WormBase

Pathology & Biotechi

Disruption phenotypei

Worms exhibit various abnormalities in axonal elongation and axonal structures (PubMed:7958904). RNAi-mediated knockdown causes abnormalities in constitutive dauer formation in daf-2 e1370 mutant including a lack of autophagosome formation (PubMed:12958363).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi37 – 41Missing : Abrogates kinase activity. 1 Publication5
Mutagenesisi39K → M in KSEX9; variable effects. 2 Publications1
Mutagenesisi39K → R: Impairs kinase activity. 2 Publications1
Mutagenesisi841R → H in E1120; paralyzed, egg laying defective and dumpy. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867851 – 856Serine/threonine-protein kinase unc-51Add BLAST856

Proteomic databases

EPDiQ23023.
PaxDbiQ23023.
PeptideAtlasiQ23023.

PTM databases

iPTMnetiQ23023.

Expressioni

Developmental stagei

During embryonic development unc-51 is expressed extensively, particularly in the head region of late embryos. In the larval stages, expression appears to be restricted to neurons.1 Publication

Gene expression databases

BgeeiWBGene00006786.

Interactioni

Subunit structurei

Interacts with unc-14 and vab-8 (PubMed:15539493). Interacts (via C-terminus) with atg-13 (PubMed:19377305).2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi45271. 2 interactors.
DIPiDIP-26124N.
IntActiQ23023. 2 interactors.
MINTiMINT-1078171.
STRINGi6239.Y60A3A.1.2.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5AZGX-ray1.81C/D353-361[»]
ProteinModelPortaliQ23023.
SMRiQ23023.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 275Protein kinasePROSITE-ProRule annotationAdd BLAST267

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni750 – 856Interaction with unc-14 and vab-8Add BLAST107

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi415 – 418Poly-Ser4
Compositional biasi429 – 436Poly-Gln8
Compositional biasi521 – 526Poly-Thr6
Compositional biasi602 – 605Poly-Glu4

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0595. Eukaryota.
ENOG410XR01. LUCA.
GeneTreeiENSGT00870000136410.
InParanoidiQ23023.
KOiK08269.
OMAiRELRPNI.
OrthoDBiEOG091G0P85.
PhylomeDBiQ23023.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR022708. Ser/Thr_kinase_C.
IPR017184. Ser/Thr_kinase_Unc51.
PfamiView protein in Pfam
PF12063. DUF3543. 1 hit.
PF00069. Pkinase. 1 hit.
PIRSFiPIRSF037369. Ser/Thr_PK_unc51. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Q23023-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQFDGFEYS KRDLLGHGAF AIVYRGRYVD RTDVPVAIKA IAKKNISKSK
60 70 80 90 100
NLLTKEIKIL KELSSLKHEN LVGLLKCTET PTHVYLVMEF CNGGDLADYL
110 120 130 140 150
QQKTTLNEDT IQHFVVQIAH ALEAINKKGI VHRDLKPQNI LLCNNSRTQN
160 170 180 190 200
PHFTDIVIKL ADFGFARFLN DGVMAATLCG SPMYMAPEVI MSMQYDAKAD
210 220 230 240 250
LWSIGTILFQ CLTGKAPFVA QTPPQLKAYY EKTRELRPNI PEWCSPNLRD
260 270 280 290 300
LLLRLLKRNA KDRISFEDFF NHPFLTSPLL PSPSKRILES ARSPLLANRR
310 320 330 340 350
IITPQSSLPV PKRAGSTKLD SPTPVRRIGE SPRVQRRVIT PGMPSPVPGA
360 370 380 390 400
PMQESTDFTF LPPRQESSPV KQVQVHTNVS PSLTTCKPVP VPSQRLTYQK
410 420 430 440 450
MEERLAAARK TAVPSSSSPT GSAVSAQHQH QHQQQQEPAS SPVVQRIERP
460 470 480 490 500
DQLPRRTTLQ DPNAHDIERM TMPNPTFVVC GSSTKPSPNN ANRVRRSTIT
510 520 530 540 550
SPADTQDMVA ADQMLSNLDP TTTTTTIPKS ATTANIQGIP RGARDRSVTS
560 570 580 590 600
PPQPTIHENE PLDNAKYQQT DVNNSPTAPT EPFIIKNQTT CSTSSTSSSV
610 620 630 640 650
VEEEEAMSLP FASGSHLAAG FKKTPAEVPM DHGALPPALD QEIVLGEEHK
660 670 680 690 700
QILAKLRFVA ELVDTLIHVA EQKDNPLASA MASRRQLLTT GTSTTNTSSP
710 720 730 740 750
YRRAEQLVVY VRALHMLSSA LLLAQTNVAN RVLHPSVAVQ QVLNQLNDKY
760 770 780 790 800
HQCLVRSQEL ASLGLPGQDP AMAVISAERI MYRHAIELCQ AAALDELFGN
810 820 830 840 850
PQLCSQRYQT AYMMLHTLAE QVNCDQDKTV LTRYKVAVEK RLRILERQGF

VAAVNT
Length:856
Mass (Da):94,893
Last modified:November 1, 1996 - v1
Checksum:i721F6F6FB0399F6E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38016 mRNA. Translation: CAA86114.1.
AL117207 Genomic DNA. Translation: CAB60406.1.
PIRiT43631.
RefSeqiNP_507869.1. NM_075468.4.
UniGeneiCel.5550.

Genome annotation databases

EnsemblMetazoaiY60A3A.1; Y60A3A.1; WBGene00006786.
GeneIDi180311.
KEGGicel:CELE_Y60A3A.1.
UCSCiY60A3A.1.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38016 mRNA. Translation: CAA86114.1.
AL117207 Genomic DNA. Translation: CAB60406.1.
PIRiT43631.
RefSeqiNP_507869.1. NM_075468.4.
UniGeneiCel.5550.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5AZGX-ray1.81C/D353-361[»]
ProteinModelPortaliQ23023.
SMRiQ23023.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi45271. 2 interactors.
DIPiDIP-26124N.
IntActiQ23023. 2 interactors.
MINTiMINT-1078171.
STRINGi6239.Y60A3A.1.2.

PTM databases

iPTMnetiQ23023.

Proteomic databases

EPDiQ23023.
PaxDbiQ23023.
PeptideAtlasiQ23023.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiY60A3A.1; Y60A3A.1; WBGene00006786.
GeneIDi180311.
KEGGicel:CELE_Y60A3A.1.
UCSCiY60A3A.1.1. c. elegans.

Organism-specific databases

CTDi180311.
WormBaseiY60A3A.1; CE24516; WBGene00006786; unc-51.

Phylogenomic databases

eggNOGiKOG0595. Eukaryota.
ENOG410XR01. LUCA.
GeneTreeiENSGT00870000136410.
InParanoidiQ23023.
KOiK08269.
OMAiRELRPNI.
OrthoDBiEOG091G0P85.
PhylomeDBiQ23023.

Enzyme and pathway databases

BRENDAi2.7.11.1. 1045.
ReactomeiR-CEL-1632852. Macroautophagy.
R-CEL-8854214. TBC/RABGAPs.
SignaLinkiQ23023.

Miscellaneous databases

PROiPR:Q23023.

Gene expression databases

BgeeiWBGene00006786.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR022708. Ser/Thr_kinase_C.
IPR017184. Ser/Thr_kinase_Unc51.
PfamiView protein in Pfam
PF12063. DUF3543. 1 hit.
PF00069. Pkinase. 1 hit.
PIRSFiPIRSF037369. Ser/Thr_PK_unc51. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUNC51_CAEEL
AccessioniPrimary (citable) accession number: Q23023
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 12, 2017
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.