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Protein

Ribokinase

Gene

TTHERM_00312950

Organism
Tetrahymena thermophila (strain SB210)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.UniRule annotation

Catalytic activityi

ATP + D-ribose = ADP + D-ribose 5-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.UniRule annotation

Enzyme regulationi

Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity.UniRule annotation

Pathwayi: D-ribose degradation

This protein is involved in step 2 of the subpathway that synthesizes D-ribose 5-phosphate from beta-D-ribopyranose.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Ribokinase (TTHERM_00312950)
This subpathway is part of the pathway D-ribose degradation, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribose 5-phosphate from beta-D-ribopyranose, the pathway D-ribose degradation and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei146SubstrateUniRule annotation1
Binding sitei191ATPUniRule annotation1
Metal bindingi265PotassiumUniRule annotation1
Metal bindingi267Potassium; via carbonyl oxygenUniRule annotation1
Active sitei271Proton acceptorUniRule annotation1
Binding sitei271SubstrateUniRule annotation1
Binding sitei307ATPUniRule annotation1
Metal bindingi313Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi316Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi318Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi322PotassiumUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi230 – 235ATPUniRule annotation6
Nucleotide bindingi270 – 271ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationImported, Transferase

Keywords - Biological processi

Carbohydrate metabolismUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PotassiumUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00916; UER00889.

Names & Taxonomyi

Protein namesi
Recommended name:
RibokinaseUniRule annotation (EC:2.7.1.15UniRule annotation)
Short name:
RKUniRule annotation
Gene namesi
ORF Names:TTHERM_00312950Imported
OrganismiTetrahymena thermophila (strain SB210)Imported
Taxonomic identifieri312017 [NCBI]
Taxonomic lineageiEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaHymenostomatidaTetrahymeninaTetrahymenidaeTetrahymena
Proteomesi
  • UP000009168 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Nucleus UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation, NucleusUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ22KI6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 325PfkBInterPro annotationAdd BLAST319

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni15 – 17Substrate bindingUniRule annotation3
Regioni43 – 47Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the carbohydrate kinase PfkB family. Ribokinase subfamily.UniRule annotation

Phylogenomic databases

InParanoidiQ22KI6.
KOiK00852.

Family and domain databases

CDDicd01174. ribokinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_01987. Ribokinase. 1 hit.
InterProiIPR011877. D_ribokin.
IPR011611. PfkB_dom.
IPR002139. Ribo/fructo_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF00294. PfkB. 1 hit.
[Graphical view]
PRINTSiPR00990. RIBOKINASE.
SUPFAMiSSF53613. SSF53613. 1 hit.

Sequencei

Sequence statusi: Complete.

Q22KI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQNTYSICV VGSGSFDLFL LVNHFPVEGE TLQANDSFIK NGGKGANQAV
60 70 80 90 100
AAAKLGAKTI FAGQFGQDKY GDGLVEEMSQ SGVDMSKVRR LANVQTGQAI
110 120 130 140 150
ITLNKKGENS IIIVGGANTH YDHLNVLPQE FKDAIQSSRI ILLQKEIPLE
160 170 180 190 200
ISKLAAIYAK QHNKLVMLDC GGRDEDIPEE LLQNIDFISP NQSELKRVMN
210 220 230 240 250
INSSDHGEIT IDEIRQQLLS KYPNLTVVLK EGSKGSAVIT KTDYLHVDSA
260 270 280 290 300
SLIKPDILNT YKIVDTTGAG DCFTAAFCVE FDKLFPQNGV QYEDALEKYK
310 320 330
KCLLFANCSA FVCITVMGAM PSMPKLESVQ QLLSKVQ
Length:337
Mass (Da):36,861
Last modified:May 29, 2007 - v2
Checksum:iDAD052BA8A22BE1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GG662498 Genomic DNA. Translation: EAR85813.2.
RefSeqiXP_001033476.2. XM_001033476.3.

Genome annotation databases

EnsemblProtistsiEAR85813; EAR85813; TTHERM_00312950.
GeneIDi7825277.
KEGGitet:TTHERM_00312950.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GG662498 Genomic DNA. Translation: EAR85813.2.
RefSeqiXP_001033476.2. XM_001033476.3.

3D structure databases

ProteinModelPortaliQ22KI6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAR85813; EAR85813; TTHERM_00312950.
GeneIDi7825277.
KEGGitet:TTHERM_00312950.

Phylogenomic databases

InParanoidiQ22KI6.
KOiK00852.

Enzyme and pathway databases

UniPathwayiUPA00916; UER00889.

Family and domain databases

CDDicd01174. ribokinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_01987. Ribokinase. 1 hit.
InterProiIPR011877. D_ribokin.
IPR011611. PfkB_dom.
IPR002139. Ribo/fructo_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF00294. PfkB. 1 hit.
[Graphical view]
PRINTSiPR00990. RIBOKINASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQ22KI6_TETTS
AccessioniPrimary (citable) accession number: Q22KI6
Entry historyi
Integrated into UniProtKB/TrEMBL: April 18, 2006
Last sequence update: May 29, 2007
Last modified: October 5, 2016
This is version 50 of the entry and version 2 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.