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Q22866

- TPM1_CAEEL

UniProt

Q22866 - TPM1_CAEEL

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Protein

Tropomyosin isoforms a/b/d/f

Gene
lev-11, tmy-1, Y105E8B.1
Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Tropomyosin, in association with the troponin complex, plays a central role in the calcium dependent regulation of muscle contraction. Involved in muscle actin filament organization and muscle arm extension and morphology. Also has a role in male mating behavior by regulating the copulatory spicules. Binds to F-actin.4 Publications

GO - Molecular functioni

  1. actin filament binding Source: UniProtKB

GO - Biological processi

  1. actin filament organization Source: UniProtKB
  2. embryo development Source: UniProtKB
  3. locomotion Source: UniProtKB
  4. negative regulation of actin filament depolymerization Source: WormBase
  5. regulation of actin filament polymerization Source: WormBase
  6. regulation of protein binding Source: WormBase
  7. spicule insertion Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Muscle protein

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tropomyosin isoforms a/b/d/f
Alternative name(s):
Levamisole resistant protein 11
Gene namesi
Name:lev-11
Synonyms:tmy-1
ORF Names:Y105E8B.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome I

Organism-specific databases

WormBaseiY105E8B.1a; CE28782; WBGene00002978; lev-11.
Y105E8B.1b; CE36223; WBGene00002978; lev-11.
Y105E8B.1d; CE29060; WBGene00002978; lev-11.
Y105E8B.1f; CE36224; WBGene00002978; lev-11.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. striated muscle thin filament Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Worms have 50-75% embryonic lethality. Those that survive body wall interference have abnormal body morphology and uncoordinated movements, and those that survive pharynx interference have deformed pharynges and gut regions.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi139 – 1391E → K in lev-11-sy558; induces spicule protraction (Prc phenotype). Prc phenotype is suppressible by food deprivation. 1 Publication
Mutagenesisi222 – 2221E → K in lev-11-rg1; disrupts most steps of male mating behavior except spicule insertion. 1 Publication
Mutagenesisi234 – 2341E → K in lev-11-x12; confers levamisole resistance. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 284284Tropomyosin isoforms a/b/d/fPRO_0000205645Add
BLAST

Proteomic databases

PaxDbiQ22866.
PRIDEiQ22866.

2D gel databases

World-2DPAGE0020:Q22866.

Expressioni

Tissue specificityi

Isoform a and isoform d are expressed in body wall muscles, vulva, anus muscles and male tail muscles. Located to the myofibrils of thin actin filaments.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi38704. 28 interactions.
DIPiDIP-27403N.
IntActiQ22866. 30 interactions.
MINTiMINT-1117333.

Structurei

3D structure databases

ProteinModelPortaliQ22866.
SMRiQ22866. Positions 1-282.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1 – 284284Add
BLAST

Domaini

The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.

Sequence similaritiesi

Belongs to the tropomyosin family.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG304012.
GeneTreeiENSGT00550000074494.
HOGENOMiHOG000231521.
InParanoidiQ22866.
KOiK10373.
OMAiEASAEKY.
PhylomeDBiQ22866.

Family and domain databases

InterProiIPR000533. Tropomyosin.
[Graphical view]
PfamiPF00261. Tropomyosin. 1 hit.
[Graphical view]
PRINTSiPR00194. TROPOMYOSIN.
PROSITEiPS00326. TROPOMYOSIN. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. Align

Isoform a (identifier: Q22866-1) [UniParc]FASTAAdd to Basket

Also known as: CeTM1, CeTMI

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDAIKKKMQA MKIEKDNALD RADAAEEKVR QITEKLERVE EELRDTQKKM    50
TQTGDDLDKA QEDLSAATSK LEEKEKTVQE AEAEVASLNR RMTLLEEELE 100
RAEERLKIAT EKLEEATHNV DESERVRKVM ENRSLQDEER ANTVEAQLKE 150
AQLLAEEADR KYDEVARKLA MVEADLERAE ERAEAGENKI VELEEELRVV 200
GNNLKSLEVS EEKALQREDS YEEQIRTVSS RLKEAETRAE FAERSVQKLQ 250
KEVDRLEDEL VHEKERYKTI SEELDSTFQE LSGY 284
Length:284
Mass (Da):33,004
Last modified:November 1, 1996 - v1
Checksum:i02271C870E23D2AB
GO
Isoform b (identifier: Q22866-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-91: Missing.
     258-284: DELVHEKERYKTISEELDSTFQELSGY → ELRDAEVLKARQLQDELDHMVQELNSV

Note: No experimental confirmation available.

Show »
Length:193
Mass (Da):22,533
Checksum:iD1C80E8B905C439B
GO
Isoform d (identifier: Q22866-2) [UniParc]FASTAAdd to Basket

Also known as: CeTM2, CeTMII

The sequence of this isoform differs from the canonical sequence as follows:
     258-284: DELVHEKERYKTISEELDSTFQELSGY → ELRDAEVLKARQLQDELDHMVQELNSV

Show »
Length:284
Mass (Da):32,937
Checksum:iD8D32CC6FB4E78F7
GO
Isoform f (identifier: Q22866-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     100-151: ERAEERLKIA...NTVEAQLKEA → NFSPRRLTAN...KRSCASLVIT
     152-284: Missing.

Note: No experimental confirmation available.

Show »
Length:151
Mass (Da):17,385
Checksum:i8C82011D1356E828
GO
Isoform e (identifier: Q27249-1) [UniParc]FASTAAdd to Basket

Also known as: CeTM3, CeTMIII

The sequence of this isoform can be found in the external entry Q27249.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:256
Mass (Da):29,632
GO
Isoform c (identifier: Q27249-2) [UniParc]FASTAAdd to Basket

Also known as: CeTM4, CeTMIV

The sequence of this isoform can be found in the external entry Q27249.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:256
Mass (Da):29,630
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9191Missing in isoform b. VSP_020645Add
BLAST
Alternative sequencei100 – 15152ERAEE…QLKEA → NFSPRRLTANTTRSPVSSPW LKLILRELRSVPRPERTRSW SLKRSCASLVIT in isoform f. VSP_020646Add
BLAST
Alternative sequencei152 – 284133Missing in isoform f. VSP_020647Add
BLAST
Alternative sequencei258 – 28427DELVH…ELSGY → ELRDAEVLKARQLQDELDHM VQELNSV in isoform b and isoform d. VSP_009425Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti277 – 2793TFQ → NLP in BAA07540. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D38539 Genomic DNA. Translation: BAA07540.1.
D38539 Genomic DNA. Translation: BAA07541.1.
D38540 mRNA. Translation: BAA07543.1.
D38541 mRNA. Translation: BAA07544.1.
AL132877 Genomic DNA. Translation: CAC70112.1.
AL132877 Genomic DNA. Translation: CAC70113.2.
AL132877 Genomic DNA. Translation: CAC70114.1.
AL132877 Genomic DNA. Translation: CAE54913.1.
PIRiS58921.
S58922.
RefSeqiNP_001021695.1. NM_001026524.5. [Q22866-1]
NP_001021696.1. NM_001026525.5. [Q22866-3]
NP_001021698.1. NM_001026527.1. [Q22866-2]
NP_001021700.1. NM_001026529.4. [Q22866-4]
UniGeneiCel.7812.

Genome annotation databases

EnsemblMetazoaiY105E8B.1a; Y105E8B.1a; WBGene00002978. [Q22866-1]
GeneIDi173319.
KEGGicel:CELE_Y105E8B.1.
UCSCiY105E8B.1e.2. c. elegans. [Q22866-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D38539 Genomic DNA. Translation: BAA07540.1 .
D38539 Genomic DNA. Translation: BAA07541.1 .
D38540 mRNA. Translation: BAA07543.1 .
D38541 mRNA. Translation: BAA07544.1 .
AL132877 Genomic DNA. Translation: CAC70112.1 .
AL132877 Genomic DNA. Translation: CAC70113.2 .
AL132877 Genomic DNA. Translation: CAC70114.1 .
AL132877 Genomic DNA. Translation: CAE54913.1 .
PIRi S58921.
S58922.
RefSeqi NP_001021695.1. NM_001026524.5. [Q22866-1 ]
NP_001021696.1. NM_001026525.5. [Q22866-3 ]
NP_001021698.1. NM_001026527.1. [Q22866-2 ]
NP_001021700.1. NM_001026529.4. [Q22866-4 ]
UniGenei Cel.7812.

3D structure databases

ProteinModelPortali Q22866.
SMRi Q22866. Positions 1-282.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 38704. 28 interactions.
DIPi DIP-27403N.
IntActi Q22866. 30 interactions.
MINTi MINT-1117333.

2D gel databases

World-2DPAGE 0020:Q22866.

Proteomic databases

PaxDbi Q22866.
PRIDEi Q22866.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai Y105E8B.1a ; Y105E8B.1a ; WBGene00002978 . [Q22866-1 ]
GeneIDi 173319.
KEGGi cel:CELE_Y105E8B.1.
UCSCi Y105E8B.1e.2. c. elegans. [Q22866-1 ]

Organism-specific databases

CTDi 173319.
WormBasei Y105E8B.1a ; CE28782 ; WBGene00002978 ; lev-11.
Y105E8B.1b ; CE36223 ; WBGene00002978 ; lev-11.
Y105E8B.1d ; CE29060 ; WBGene00002978 ; lev-11.
Y105E8B.1f ; CE36224 ; WBGene00002978 ; lev-11.

Phylogenomic databases

eggNOGi NOG304012.
GeneTreei ENSGT00550000074494.
HOGENOMi HOG000231521.
InParanoidi Q22866.
KOi K10373.
OMAi EASAEKY.
PhylomeDBi Q22866.

Miscellaneous databases

NextBioi 879141.

Family and domain databases

InterProi IPR000533. Tropomyosin.
[Graphical view ]
Pfami PF00261. Tropomyosin. 1 hit.
[Graphical view ]
PRINTSi PR00194. TROPOMYOSIN.
PROSITEi PS00326. TROPOMYOSIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genome structure, mapping and expression of the tropomyosin gene tmy-1 of Caenorhabditis elegans."
    Kagawa H., Sugimoto K., Matsumoto H., Inoue T., Imadzu H., Takuwa K., Sakube Y.
    J. Mol. Biol. 251:603-613(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS A AND D), TISSUE SPECIFICITY.
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  3. "Mutations and expressions of the tropomyosin gene and the troponin C gene of Caenorhabditis elegans."
    Kagawa H., Takuwa K., Sakube Y.
    Cell Struct. Funct. 22:213-218(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF GLU-234.
  4. "Tropomyosin inhibits ADF/cofilin-dependent actin filament dynamics."
    Ono S., Ono K.
    J. Cell Biol. 156:1065-1076(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  5. Erratum
    Ono S., Ono K.
    J. Cell Biol. 157:727-727(2002)
  6. "Muscle arm development in Caenorhabditis elegans."
    Dixon S.J., Roy P.J.
    Development 132:3079-3092(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  7. "Integration of male mating and feeding behaviors in Caenorhabditis elegans."
    Gruninger T.R., Gualberto D.G., LeBoeuf B., Garcia L.R.
    J. Neurosci. 26:169-179(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF GLU-139 AND GLU-222.

Entry informationi

Entry nameiTPM1_CAEEL
AccessioniPrimary (citable) accession number: Q22866
Secondary accession number(s): Q22865
, Q27284, Q7K6W7, Q95Q31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi