Q22866 (TPM1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tropomyosin isoforms a/b/d/f

Alternative name(s):
Levamisole resistant protein 11

Gene names

Name:	lev-11
Synonyms:	tmy-1
ORF Names:	Y105E8B.1

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Tropomyosin, in association with the troponin complex, plays a central role in the calcium dependent regulation of muscle contraction. Involved in muscle actin filament organization and muscle arm extension and morphology. Also has a role in male mating behavior by regulating the copulatory spicules. Binds to F-actin. Ref.3 Ref.4 Ref.6 Ref.7
Subcellular location	Cytoplasm Ref.4.
Tissue specificity	Isoform a and isoform d are expressed in body wall muscles, vulva, anus muscles and male tail muscles. Located to the myofibrils of thin actin filaments. Ref.1 Ref.4
Domain	The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.
Disruption phenotype	Worms have 50-75% embryonic lethality. Those that survive body wall interference have abnormal body morphology and uncoordinated movements, and those that survive pharynx interference have deformed pharynges and gut regions. Ref.6
Sequence similarities	Belongs to the tropomyosin family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Ligand	Actin-binding
Molecular function	Muscle protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin filament organization Inferred from mutant phenotype Ref.4. Source: UniProtKB embryo development Inferred from mutant phenotype Ref.4. Source: UniProtKB locomotion Inferred from mutant phenotype Ref.4. Source: UniProtKB negative regulation of actin filament depolymerization Inferred from direct assay PubMed 18984629. Source: WormBase regulation of actin filament polymerization Inferred from direct assay Ref.4. Source: WormBase regulation of protein binding Inferred from direct assay Ref.4. Source: WormBase spicule insertion Inferred from mutant phenotype Ref.7. Source: UniProtKB
Cellular_component	striated muscle thin filament Inferred from direct assay Ref.4. Source: WormBase
Molecular_function	actin filament binding Inferred from direct assay Ref.4. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
gei-4	Q9TZ38	1	EBI-329542,EBI-329192

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: Q22866-1)

Also known as: CeTM1; CeTMI;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: Q22866-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-91: Missing.
     258-284: DELVHEKERYKTISEELDSTFQELSGY → ELRDAEVLKARQLQDELDHMVQELNSV

Note: No experimental confirmation available.

Isoform d (identifier: Q22866-2)

Also known as: CeTM2; CeTMII;

The sequence of this isoform differs from the canonical sequence as follows:
     258-284: DELVHEKERYKTISEELDSTFQELSGY → ELRDAEVLKARQLQDELDHMVQELNSV

Isoform f (identifier: Q22866-4)

The sequence of this isoform differs from the canonical sequence as follows:
     100-151: ERAEERLKIA...NTVEAQLKEA → NFSPRRLTAN...KRSCASLVIT
     152-284: Missing.

Note: No experimental confirmation available.

Isoform e (identifier: Q27249-1)

Also known as: CeTM3; CeTMIII;

The sequence of this isoform can be found in the external entry Q27249.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform c (identifier: Q27249-2)

Also known as: CeTM4; CeTMIV;

The sequence of this isoform can be found in the external entry Q27249.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Tropomyosin isoforms a/b/d/f		PRO_0000205645
Regions
	Coiled coil	1 – 284	284
Natural variations
	Alternative sequence	1 – 91	91	Missing in isoform b.		VSP_020645
	Alternative sequence	100 – 151	52	ERAEE…QLKEA → NFSPRRLTANTTRSPVSSPW LKLILRELRSVPRPERTRSW SLKRSCASLVIT in isoform f.		VSP_020646
	Alternative sequence	152 – 284	133	Missing in isoform f.		VSP_020647
	Alternative sequence	258 – 284	27	DELVH…ELSGY → ELRDAEVLKARQLQDELDHM VQELNSV in isoform b and isoform d.		VSP_009425
Experimental info
	Mutagenesis	139	1	E → K in lev-11-sy558; induces spicule protraction (Prc phenotype). Prc phenotype is suppressible by food deprivation. Ref.7
	Mutagenesis	222	1	E → K in lev-11-rg1; disrupts most steps of male mating behavior except spicule insertion. Ref.7
	Mutagenesis	234	1	E → K in lev-11-x12; confers levamisole resistance. Ref.3
	Sequence conflict	277 – 279	3	TFQ → NLP in BAA07540. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform a (CeTM1) (CeTMI) [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 02271C870E23D2AB	FASTA	284	33,004
	10 20 30 40 50 60 MDAIKKKMQA MKIEKDNALD RADAAEEKVR QITEKLERVE EELRDTQKKM TQTGDDLDKA 70 80 90 100 110 120 QEDLSAATSK LEEKEKTVQE AEAEVASLNR RMTLLEEELE RAEERLKIAT EKLEEATHNV 130 140 150 160 170 180 DESERVRKVM ENRSLQDEER ANTVEAQLKE AQLLAEEADR KYDEVARKLA MVEADLERAE 190 200 210 220 230 240 ERAEAGENKI VELEEELRVV GNNLKSLEVS EEKALQREDS YEEQIRTVSS RLKEAETRAE 250 260 270 280 FAERSVQKLQ KEVDRLEDEL VHEKERYKTI SEELDSTFQE LSGY
	Isoform b [UniParc]. Checksum: D1C80E8B905C439B	FASTA	193	22,533
	10 20 30 40 50 60 MTLLEEELER AEERLKIATE KLEEATHNVD ESERVRKVME NRSLQDEERA NTVEAQLKEA 70 80 90 100 110 120 QLLAEEADRK YDEVARKLAM VEADLERAEE RAEAGENKIV ELEEELRVVG NNLKSLEVSE 130 140 150 160 170 180 EKALQREDSY EEQIRTVSSR LKEAETRAEF AERSVQKLQK EVDRLEELRD AEVLKARQLQ 190 DELDHMVQEL NSV
	Isoform d (CeTM2) (CeTMII) [UniParc]. Checksum: D8D32CC6FB4E78F7	FASTA	284	32,937
	10 20 30 40 50 60 MDAIKKKMQA MKIEKDNALD RADAAEEKVR QITEKLERVE EELRDTQKKM TQTGDDLDKA 70 80 90 100 110 120 QEDLSAATSK LEEKEKTVQE AEAEVASLNR RMTLLEEELE RAEERLKIAT EKLEEATHNV 130 140 150 160 170 180 DESERVRKVM ENRSLQDEER ANTVEAQLKE AQLLAEEADR KYDEVARKLA MVEADLERAE 190 200 210 220 230 240 ERAEAGENKI VELEEELRVV GNNLKSLEVS EEKALQREDS YEEQIRTVSS RLKEAETRAE 250 260 270 280 FAERSVQKLQ KEVDRLEELR DAEVLKARQL QDELDHMVQE LNSV
	Isoform f [UniParc]. Checksum: 8C82011D1356E828	FASTA	151	17,385
	10 20 30 40 50 60 MDAIKKKMQA MKIEKDNALD RADAAEEKVR QITEKLERVE EELRDTQKKM TQTGDDLDKA 70 80 90 100 110 120 QEDLSAATSK LEEKEKTVQE AEAEVASLNR RMTLLEEELN FSPRRLTANT TRSPVSSPWL 130 140 150 KLILRELRSV PRPERTRSWS LKRSCASLVI T
	Isoform e (CeTM3) (CeTMIII) [UniParc]. See Q27249.		–	–
	Isoform c (CeTM4) (CeTMIV) [UniParc]. See Q27249.		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome structure, mapping and expression of the tropomyosin gene tmy-1 of Caenorhabditis elegans." Kagawa H., Sugimoto K., Matsumoto H., Inoue T., Imadzu H., Takuwa K., Sakube Y. J. Mol. Biol. 251:603-613(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS A AND D), TISSUE SPECIFICITY. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[3]	"Mutations and expressions of the tropomyosin gene and the troponin C gene of Caenorhabditis elegans." Kagawa H., Takuwa K., Sakube Y. Cell Struct. Funct. 22:213-218(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF GLU-234.
[4]	"Tropomyosin inhibits ADF/cofilin-dependent actin filament dynamics." Ono S., Ono K. J. Cell Biol. 156:1065-1076(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]	Erratum Ono S., Ono K. J. Cell Biol. 157:727-727(2002)
[6]	"Muscle arm development in Caenorhabditis elegans." Dixon S.J., Roy P.J. Development 132:3079-3092(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[7]	"Integration of male mating and feeding behaviors in Caenorhabditis elegans." Gruninger T.R., Gualberto D.G., LeBoeuf B., Garcia L.R. J. Neurosci. 26:169-179(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF GLU-139 AND GLU-222.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D38539 Genomic DNA. Translation: BAA07540.1. D38539 Genomic DNA. Translation: BAA07541.1. D38540 mRNA. Translation: BAA07543.1. D38541 mRNA. Translation: BAA07544.1. AL132877 Genomic DNA. Translation: CAC70112.1. AL132877 Genomic DNA. Translation: CAC70113.2. AL132877 Genomic DNA. Translation: CAC70114.1. AL132877 Genomic DNA. Translation: CAE54913.1.
PIR	S58921. S58922.
RefSeq	NP_001021695.1. NM_001026524.5. NP_001021696.1. NM_001026525.5. NP_001021698.1. NM_001026527.1. NP_001021700.1. NM_001026529.4.
UniGene	Cel.7812.
3D structure databases
ProteinModelPortal	Q22866.
SMR	Q22866. Positions 1-282.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-27403N.
IntAct	Q22866. 29 interactions.
MINT	MINT-1117333.
2D gel databases
World-2DPAGE	0020:Q22866.
Proteomic databases
PaxDb	Q22866.
PRIDE	Q22866.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	Y105E8B.1a; Y105E8B.1a; Y105E8B.1.
GeneID	173319.
KEGG	cel:CELE_Y105E8B.1.
UCSC	Y105E8B.1e.2. c. elegans.
Organism-specific databases
CTD	173319.
WormBase	Y105E8B.1a; CE28782; WBGene00002978; lev-11. Y105E8B.1b; CE36223; WBGene00002978; lev-11. Y105E8B.1d; CE29060; WBGene00002978; lev-11. Y105E8B.1f; CE36224; WBGene00002978; lev-11.
Phylogenomic databases
eggNOG	NOG304012.
GeneTree	ENSGT00550000074494.
HOGENOM	HOG000231521.
InParanoid	Q22866.
KO	K09290.
OMA	DSYEETI.
Gene expression databases
ArrayExpress	Q22866.
Family and domain databases
InterPro	IPR000533. Tropomyosin. [Graphical view]
Pfam	PF00261. Tropomyosin. 1 hit. [Graphical view]
PRINTS	PR00194. TROPOMYOSIN.
PROSITE	PS00326. TROPOMYOSIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	879141.

Entry information

Entry name

TPM1_CAEEL

Accession

Primary (citable) accession number: Q22866
Secondary accession number(s): Q22865 , Q27284, Q7K6W7, Q95Q31

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project