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Protein

Myotubularin-related protein 3

Gene

mtm-3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Preferentially dephosphorylates phosphatidylinositol 3-phosphate (PI3P), and has some activity towards phosphatidylinositol 3,5-bisphosphate (PI35P). May also dephosphorylate proteins phosphorylated on Ser, Thr, and Tyr residues (By similarity). Thought to have a role in maintenance of muscle function. Involved in locomotion and lifespan determination.By similarity1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotationBy similarity
1-phosphatidyl-1D-myo-inositol 3-phosphate + H2O = 1-phosphatidyl-1D-myo-inositol + phosphate.1 Publication
1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate.1 Publication

Enzyme regulationi

Inhibited by sodium vanadate and peroxide.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei463Phosphocysteine intermediatePROSITE-ProRule annotation1
Binding sitei509SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri818 – 883FYVE-typePROSITE-ProRule annotationAdd BLAST66

GO - Molecular functioni

GO - Biological processi

  • determination of adult lifespan Source: WormBase
  • inositol phosphate dephosphorylation Source: WormBase
  • locomotion Source: WormBase
  • negative regulation of engulfment of apoptotic cell Source: WormBase
  • phosphatidylinositol dephosphorylation Source: UniProtKB
  • positive regulation of endocytosis Source: WormBase
  • regulation of oviposition Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase

Keywords - Biological processi

Lipid metabolism

Keywords - Ligandi

Lipid-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-CEL-1632852. Macroautophagy.
R-CEL-1660499. Synthesis of PIPs at the plasma membrane.
R-CEL-1660516. Synthesis of PIPs at the early endosome membrane.
R-CEL-1660517. Synthesis of PIPs at the late endosome membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Myotubularin-related protein 3 (EC:3.1.3.48By similarity)
Short name:
ceMTM3
Alternative name(s):
Myotubularin homologous protein 1
Short name:
ceMTMH1
Phosphatidylinositol-3,5-bisphosphate 3-phosphatase (EC:3.1.3.951 Publication)
Phosphatidylinositol-3-phosphate phosphatase (EC:3.1.3.641 Publication)
Gene namesi
Name:mtm-3
ORF Names:T24A11.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiT24A11.1a; CE28087; WBGene00003476; mtm-3.
T24A11.1b; CE42568; WBGene00003476; mtm-3.

Subcellular locationi

  • Cytoplasm By similarity
  • Membrane By similarity; Peripheral membrane protein By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Worms exhibit a 2-fold increase in PI3P, sluggish body movement with progressive deterioration and defects in gestation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000949471 – 1006Myotubularin-related protein 3Add BLAST1006

Proteomic databases

EPDiQ22712.
PaxDbiQ22712.
PeptideAtlasiQ22712.
PRIDEiQ22712.

Expressioni

Tissue specificityi

Expressed in the body wall muscle, intestine and in eggs (PubMed:18393358). Expressed in head neurons (PubMed:12788949).2 Publications

Developmental stagei

Expressed most strongly in the egg with expression present in L1 but reducing through to L3 where it is very weak. No expression was found in L4. Strong expression is also seen in egg-laying adults and post-reproductive adults.1 Publication

Gene expression databases

BgeeiWBGene00003476.

Interactioni

Protein-protein interaction databases

BioGridi40728. 3 interactors.
STRINGi6239.T24A11.1a.

Structurei

3D structure databases

ProteinModelPortaliQ22712.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini224 – 630Myotubularin phosphatasePROSITE-ProRule annotationAdd BLAST407

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni377 – 380Substrate bindingBy similarity4
Regioni402 – 403Substrate bindingBy similarity2
Regioni463 – 469Substrate bindingBy similarity7

Domaini

Interacts with phosphatidylinositol 3-phosphate (PI3P) via the FYVE-type domain.1 Publication

Sequence similaritiesi

Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation
Contains 1 myotubularin phosphatase domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri818 – 883FYVE-typePROSITE-ProRule annotationAdd BLAST66

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410INM7. Eukaryota.
ENOG410YU6P. LUCA.
GeneTreeiENSGT00760000118832.
HOGENOMiHOG000018814.
InParanoidiQ22712.
KOiK18082.
OMAiAKHAYSG.
OrthoDBiEOG091G03HG.
PhylomeDBiQ22712.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR010569. Myotubularin-like_Pase_dom.
IPR030564. Myotubularin_fam.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10807. PTHR10807. 4 hits.
PfamiPF01363. FYVE. 1 hit.
PF06602. Myotub-related. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
SM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEiPS51339. PPASE_MYOTUBULARIN. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q22712-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTVTSSAAID IGGGGGGRRS DRLDSDRTSE DMSFIASPAN ESFQIGASFV
60 70 80 90 100
DVQNESSGSI DTATATLHEL NYTFGMPPVT EESENMPQNY ETVVELLPGE
110 120 130 140 150
ERAPINKLTE FPIEGGSLFV TNFRIVVILK DKEVEEALRF LVFPLQDIEQ
160 170 180 190 200
IDLAIPAFIH LSLKIGRMFT ICFKTAEDAA LVHKILYTAF QRLNRPISSI
210 220 230 240 250
YTSRPQDWTS KNTDNPMQSL NAFAWKFSEA VDELDRDGKL PSWLLRADSV
260 270 280 290 300
AQEITHIDFN RLGMSEHFQI SSVNENFEVC PTYPEKIIVP KGITDDDIRK
310 320 330 340 350
GAPYRSIGRF PAVIWRCRKT RAVLMRSSQP QVGILSWRNP TDEKIIEEAV
360 370 380 390 400
KASRIEGEEK KQFIIMDARG YTSAFANRAR SGGFENTEYY QQAKLEFLGL
410 420 430 440 450
PNIHAVRGSF NNVRTMLHNL GPNEQLLTSL QTTGWLLNLS NLLVNAANCA
460 470 480 490 500
DHLSKGHSVL VHCSDGWDRT TQVTTLAKIM LDEYYRTVKG FEELIRRDWI
510 520 530 540 550
AFGHKLYDRQ LVAFGNWGTS DERSPVFLQF LEAVRHLQRE QPTLFQFTHA
560 570 580 590 600
YLIKLAKHAY SGLFGSFLFN SHKERREAME KCKGTLVDIW RFIGPHNEEY
610 620 630 640 650
VNQSFDEHYT GAVKPVNVSV INLRVWHEVF ADEEEHYTQI FSPKEERPLS
660 670 680 690 700
GCTTPMNTST STNLVKSKSS ESINSLNVDG SAKESSQQHP TCSTTPSDNT
710 720 730 740 750
NSLPMSTSFI QQSLYQPKVR GVAAIDRDGV IRFEDDEQAM LRKKNKLRAE
760 770 780 790 800
EIRRKDEKIE ELRRRAVLDT NKVSPGQRQS YSESDVETTG TLERVMSDVS
810 820 830 840 850
MVDPVNELPH FKPNTTWEGE SGHCAYCKKE FNKLSVYVED RQHHCRNCGR
860 870 880 890 900
VVCEDCSKNR FSVIEEGKSV QKRACDSCYD SMHETDLKLS SSSTTTTSSS
910 920 930 940 950
TKIENDSNVP GLDNNSDNVS ENVSENAIPD IIVEEKEAED PIKEAESPSK
960 970 980 990 1000
ETKCPKTLRN FISFSPKSSM RKNKVHSRDP LKSIDEGSSS QQAESDDVLD

VNEQPL
Length:1,006
Mass (Da):113,614
Last modified:September 2, 2008 - v2
Checksum:iBEFC03296E74DB1A
GO
Isoform b (identifier: Q22712-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     41-44: Missing.
     891-965: SSSTTTTSSS...KTLRNFISFS → RATSLSEMSS...RHSSTQAVKG
     966-1006: Missing.

Show »
Length:961
Mass (Da):108,189
Checksum:i7EAC08FCD831B9C9
GO

Sequence cautioni

The sequence AAC78763 differs from that shown. Reason: Frameshift at position 913.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03503541 – 44Missing in isoform b. 1 Publication4
Alternative sequenceiVSP_035036891 – 965SSSTT…FISFS → RATSLSEMSSFDSFGPSSPP ASSTSSSSLNMLASMSPTRP QPIPISCKSSSNSISSPRPS PGEFSRHSSTQAVKG in isoform b. 1 PublicationAdd BLAST75
Alternative sequenceiVSP_035037966 – 1006Missing in isoform b. 1 PublicationAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031519 mRNA. Translation: AAC78763.1. Frameshift.
DQ988041 mRNA. Translation: ABK59970.1.
Z49072 Genomic DNA. Translation: CAA88884.2.
Z49072 Genomic DNA. Translation: CAB61017.2.
PIRiT25215.
RefSeqiNP_001022794.2. NM_001027623.5. [Q22712-1]
NP_497766.3. NM_065365.6. [Q22712-2]
UniGeneiCel.18074.

Genome annotation databases

EnsemblMetazoaiT24A11.1a; T24A11.1a; WBGene00003476. [Q22712-1]
GeneIDi175490.
KEGGicel:CELE_T24A11.1.
UCSCiT24A11.1b. c. elegans.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031519 mRNA. Translation: AAC78763.1. Frameshift.
DQ988041 mRNA. Translation: ABK59970.1.
Z49072 Genomic DNA. Translation: CAA88884.2.
Z49072 Genomic DNA. Translation: CAB61017.2.
PIRiT25215.
RefSeqiNP_001022794.2. NM_001027623.5. [Q22712-1]
NP_497766.3. NM_065365.6. [Q22712-2]
UniGeneiCel.18074.

3D structure databases

ProteinModelPortaliQ22712.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi40728. 3 interactors.
STRINGi6239.T24A11.1a.

Proteomic databases

EPDiQ22712.
PaxDbiQ22712.
PeptideAtlasiQ22712.
PRIDEiQ22712.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT24A11.1a; T24A11.1a; WBGene00003476. [Q22712-1]
GeneIDi175490.
KEGGicel:CELE_T24A11.1.
UCSCiT24A11.1b. c. elegans.

Organism-specific databases

CTDi175490.
WormBaseiT24A11.1a; CE28087; WBGene00003476; mtm-3.
T24A11.1b; CE42568; WBGene00003476; mtm-3.

Phylogenomic databases

eggNOGiENOG410INM7. Eukaryota.
ENOG410YU6P. LUCA.
GeneTreeiENSGT00760000118832.
HOGENOMiHOG000018814.
InParanoidiQ22712.
KOiK18082.
OMAiAKHAYSG.
OrthoDBiEOG091G03HG.
PhylomeDBiQ22712.

Enzyme and pathway databases

ReactomeiR-CEL-1632852. Macroautophagy.
R-CEL-1660499. Synthesis of PIPs at the plasma membrane.
R-CEL-1660516. Synthesis of PIPs at the early endosome membrane.
R-CEL-1660517. Synthesis of PIPs at the late endosome membrane.

Miscellaneous databases

PROiQ22712.

Gene expression databases

BgeeiWBGene00003476.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR010569. Myotubularin-like_Pase_dom.
IPR030564. Myotubularin_fam.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10807. PTHR10807. 4 hits.
PfamiPF01363. FYVE. 1 hit.
PF06602. Myotub-related. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
SM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEiPS51339. PPASE_MYOTUBULARIN. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMTMR3_CAEEL
AccessioniPrimary (citable) accession number: Q22712
Secondary accession number(s): A8NBE6, Q9U360, Q9XTK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 2, 2008
Last modified: November 2, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.