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Q22712

- MTMR3_CAEEL

UniProt

Q22712 - MTMR3_CAEEL

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Protein

Myotubularin-related protein 3

Gene
mtm-3, T24A11.1
Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Preferentially dephosphorylates phosphatidylinositol 3-phosphate (PI3P), and has some activity towards phosphatidylinositol 3,5-bisphosphate (PI35P). Thought to have a role in maintenance of muscle function. Involved in locomotion and lifespan determination.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Enzyme regulationi

Inhibited by sodium vanadate and peroxide.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei463 – 4631Phosphocysteine intermediate By similarity
Binding sitei509 – 5091Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri818 – 88366FYVE-typeAdd
BLAST

GO - Molecular functioni

  1. inositol bisphosphate phosphatase activity Source: WormBase
  2. lipid binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
  4. phosphatidylinositol trisphosphate phosphatase activity Source: WormBase
  5. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. determination of adult lifespan Source: WormBase
  2. inositol phosphate dephosphorylation Source: WormBase
  3. lipid metabolic process Source: UniProtKB-KW
  4. locomotion Source: WormBase
  5. negative regulation of engulfment of apoptotic cell Source: WormBase
  6. positive regulation of endocytosis Source: WormBase
  7. regulation of oviposition Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase

Keywords - Biological processi

Lipid metabolism

Keywords - Ligandi

Lipid-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_197727. Synthesis of PIPs at the early endosome membrane.
REACT_197730. Synthesis of PIPs at the late endosome membrane.
REACT_197739. Synthesis of PIPs at the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Myotubularin-related protein 3 (EC:3.1.3.48)
Short name:
ceMTM3
Alternative name(s):
Myotubularin homologous protein 1
Short name:
ceMTMH1
Gene namesi
Name:mtm-3
ORF Names:T24A11.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome III

Organism-specific databases

WormBaseiT24A11.1a; CE28087; WBGene00003476; mtm-3.
T24A11.1b; CE42568; WBGene00003476; mtm-3.

Pathology & Biotechi

Disruption phenotypei

Worms exhibit a 2-fold increase in PI3P, sluggish body movement with progressive deterioration and defects in gestation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10061006Myotubularin-related protein 3PRO_0000094947Add
BLAST

Proteomic databases

PaxDbiQ22712.
PRIDEiQ22712.

Expressioni

Tissue specificityi

Expressed in the body wall muscle, intestine and in eggs.1 Publication

Developmental stagei

Expressed most strongly in the egg with expression present in L1 but reducing through to L3 where it is very weak. No expression was found in L4. Strong expression is also seen in egg-laying adults and post-reproductive adults.1 Publication

Interactioni

Subunit structurei

Interacts with phosphatidylinositol 3-phosphate (PI3P) via the FYVE-type domain.

Protein-protein interaction databases

BioGridi40728. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ22712.
SMRiQ22712. Positions 257-626, 841-887.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini224 – 630407Myotubularin phosphataseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni377 – 3804Substrate binding By similarity
Regioni402 – 4032Substrate binding By similarity
Regioni463 – 4697Substrate binding By similarity

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri818 – 88366FYVE-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG322789.
GeneTreeiENSGT00740000115188.
HOGENOMiHOG000018814.
InParanoidiQ22712.
KOiK18082.
OMAiAKHAYSG.
OrthoDBiEOG7PVWNJ.
PhylomeDBiQ22712.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR010569. Myotubularin-like_Pase_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR016130. Tyr_Pase_AS.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
PF06602. Myotub-related. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEiPS51339. PPASE_MYOTUBULARIN. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform a (identifier: Q22712-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MTVTSSAAID IGGGGGGRRS DRLDSDRTSE DMSFIASPAN ESFQIGASFV     50
DVQNESSGSI DTATATLHEL NYTFGMPPVT EESENMPQNY ETVVELLPGE 100
ERAPINKLTE FPIEGGSLFV TNFRIVVILK DKEVEEALRF LVFPLQDIEQ 150
IDLAIPAFIH LSLKIGRMFT ICFKTAEDAA LVHKILYTAF QRLNRPISSI 200
YTSRPQDWTS KNTDNPMQSL NAFAWKFSEA VDELDRDGKL PSWLLRADSV 250
AQEITHIDFN RLGMSEHFQI SSVNENFEVC PTYPEKIIVP KGITDDDIRK 300
GAPYRSIGRF PAVIWRCRKT RAVLMRSSQP QVGILSWRNP TDEKIIEEAV 350
KASRIEGEEK KQFIIMDARG YTSAFANRAR SGGFENTEYY QQAKLEFLGL 400
PNIHAVRGSF NNVRTMLHNL GPNEQLLTSL QTTGWLLNLS NLLVNAANCA 450
DHLSKGHSVL VHCSDGWDRT TQVTTLAKIM LDEYYRTVKG FEELIRRDWI 500
AFGHKLYDRQ LVAFGNWGTS DERSPVFLQF LEAVRHLQRE QPTLFQFTHA 550
YLIKLAKHAY SGLFGSFLFN SHKERREAME KCKGTLVDIW RFIGPHNEEY 600
VNQSFDEHYT GAVKPVNVSV INLRVWHEVF ADEEEHYTQI FSPKEERPLS 650
GCTTPMNTST STNLVKSKSS ESINSLNVDG SAKESSQQHP TCSTTPSDNT 700
NSLPMSTSFI QQSLYQPKVR GVAAIDRDGV IRFEDDEQAM LRKKNKLRAE 750
EIRRKDEKIE ELRRRAVLDT NKVSPGQRQS YSESDVETTG TLERVMSDVS 800
MVDPVNELPH FKPNTTWEGE SGHCAYCKKE FNKLSVYVED RQHHCRNCGR 850
VVCEDCSKNR FSVIEEGKSV QKRACDSCYD SMHETDLKLS SSSTTTTSSS 900
TKIENDSNVP GLDNNSDNVS ENVSENAIPD IIVEEKEAED PIKEAESPSK 950
ETKCPKTLRN FISFSPKSSM RKNKVHSRDP LKSIDEGSSS QQAESDDVLD 1000
VNEQPL 1006
Length:1,006
Mass (Da):113,614
Last modified:September 2, 2008 - v2
Checksum:iBEFC03296E74DB1A
GO
Isoform b (identifier: Q22712-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     41-44: Missing.
     891-965: SSSTTTTSSS...KTLRNFISFS → RATSLSEMSS...RHSSTQAVKG
     966-1006: Missing.

Show »
Length:961
Mass (Da):108,189
Checksum:i7EAC08FCD831B9C9
GO

Sequence cautioni

The sequence AAC78763.1 differs from that shown. Reason: Frameshift at position 913.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei41 – 444Missing in isoform b. VSP_035035
Alternative sequencei891 – 96575SSSTT…FISFS → RATSLSEMSSFDSFGPSSPP ASSTSSSSLNMLASMSPTRP QPIPISCKSSSNSISSPRPS PGEFSRHSSTQAVKG in isoform b. VSP_035036Add
BLAST
Alternative sequencei966 – 100641Missing in isoform b. VSP_035037Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF031519 mRNA. Translation: AAC78763.1. Frameshift.
DQ988041 mRNA. Translation: ABK59970.1.
Z49072 Genomic DNA. Translation: CAA88884.2.
Z49072 Genomic DNA. Translation: CAB61017.2.
PIRiT25215.
RefSeqiNP_001022794.2. NM_001027623.5. [Q22712-1]
NP_497766.3. NM_065365.6. [Q22712-2]
UniGeneiCel.18074.

Genome annotation databases

EnsemblMetazoaiT24A11.1a; T24A11.1a; WBGene00003476. [Q22712-1]
GeneIDi175490.
KEGGicel:CELE_T24A11.1.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF031519 mRNA. Translation: AAC78763.1 . Frameshift.
DQ988041 mRNA. Translation: ABK59970.1 .
Z49072 Genomic DNA. Translation: CAA88884.2 .
Z49072 Genomic DNA. Translation: CAB61017.2 .
PIRi T25215.
RefSeqi NP_001022794.2. NM_001027623.5. [Q22712-1 ]
NP_497766.3. NM_065365.6. [Q22712-2 ]
UniGenei Cel.18074.

3D structure databases

ProteinModelPortali Q22712.
SMRi Q22712. Positions 257-626, 841-887.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 40728. 3 interactions.

Proteomic databases

PaxDbi Q22712.
PRIDEi Q22712.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai T24A11.1a ; T24A11.1a ; WBGene00003476 . [Q22712-1 ]
GeneIDi 175490.
KEGGi cel:CELE_T24A11.1.

Organism-specific databases

CTDi 175490.
WormBasei T24A11.1a ; CE28087 ; WBGene00003476 ; mtm-3.
T24A11.1b ; CE42568 ; WBGene00003476 ; mtm-3.

Phylogenomic databases

eggNOGi NOG322789.
GeneTreei ENSGT00740000115188.
HOGENOMi HOG000018814.
InParanoidi Q22712.
KOi K18082.
OMAi AKHAYSG.
OrthoDBi EOG7PVWNJ.
PhylomeDBi Q22712.

Enzyme and pathway databases

Reactomei REACT_197727. Synthesis of PIPs at the early endosome membrane.
REACT_197730. Synthesis of PIPs at the late endosome membrane.
REACT_197739. Synthesis of PIPs at the plasma membrane.

Miscellaneous databases

NextBioi 888376.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR010569. Myotubularin-like_Pase_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR016130. Tyr_Pase_AS.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view ]
Pfami PF01363. FYVE. 1 hit.
PF06602. Myotub-related. 1 hit.
[Graphical view ]
SMARTi SM00064. FYVE. 1 hit.
[Graphical view ]
SUPFAMi SSF52799. SSF52799. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEi PS51339. PPASE_MYOTUBULARIN. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the myotubularin dual specificity phosphatase gene family from yeast to human."
    Laporte J., Blondeau F., Buj-Bello A., Tentler D., Kretz C., Dahl N., Mandel J.-L.
    Hum. Mol. Genet. 7:1703-1712(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
  2. "Characterization and functional studies of a FYVE domain-containing phosphatase in C. elegans."
    Ma J., Zeng F., Ho W.T., Teng L., Li Q., Fu X., Zhao Z.J.
    J. Cell. Biochem. 104:1843-1852(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), FUNCTION, PHOSPHATIDYLINOSITOL 3-PHOSPHATE-BINDING, ENZYME REGULATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.

Entry informationi

Entry nameiMTMR3_CAEEL
AccessioniPrimary (citable) accession number: Q22712
Secondary accession number(s): A8NBE6, Q9U360, Q9XTK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 2, 2008
Last modified: September 3, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi