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Q22712 (MTMR3_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myotubularin-related protein 3

Short name=ceMTM3
EC=3.1.3.48
Alternative name(s):
Myotubularin homologous protein 1
Short name=ceMTMH1
Gene names
Name:mtm-3
ORF Names:T24A11.1
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length1006 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Preferentially dephosphorylates phosphatidylinositol 3-phosphate (PI3P), and has some activity towards phosphatidylinositol 3,5-bisphosphate (PI35P). Thought to have a role in maintenance of muscle function. Involved in locomotion and lifespan determination. Ref.2

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Enzyme regulation

Inhibited by sodium vanadate and peroxide. Ref.2

Subunit structure

Interacts with phosphatidylinositol 3-phosphate (PI3P) via the FYVE-type domain.

Tissue specificity

Expressed in the body wall muscle, intestine and in eggs. Ref.2

Developmental stage

Expressed most strongly in the egg with expression present in L1 but reducing through to L3 where it is very weak. No expression was found in L4. Strong expression is also seen in egg-laying adults and post-reproductive adults. Ref.2

Disruption phenotype

Worms exhibit a 2-fold increase in PI3P, sluggish body movement with progressive deterioration and defects in gestation. Ref.2

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.

Contains 1 FYVE-type zinc finger.

Contains 1 myotubularin phosphatase domain.

Sequence caution

The sequence AAC78763.1 differs from that shown. Reason: Frameshift at position 913.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: Q22712-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform b (identifier: Q22712-2)

The sequence of this isoform differs from the canonical sequence as follows:
     41-44: Missing.
     891-965: SSSTTTTSSS...KTLRNFISFS → RATSLSEMSS...RHSSTQAVKG
     966-1006: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10061006Myotubularin-related protein 3
PRO_0000094947

Regions

Domain224 – 630407Myotubularin phosphatase
Zinc finger818 – 88366FYVE-type
Region377 – 3804Substrate binding By similarity
Region402 – 4032Substrate binding By similarity
Region463 – 4697Substrate binding By similarity

Sites

Active site4631Phosphocysteine intermediate By similarity
Binding site5091Substrate By similarity

Natural variations

Alternative sequence41 – 444Missing in isoform b.
VSP_035035
Alternative sequence891 – 96575SSSTT…FISFS → RATSLSEMSSFDSFGPSSPP ASSTSSSSLNMLASMSPTRP QPIPISCKSSSNSISSPRPS PGEFSRHSSTQAVKG in isoform b.
VSP_035036
Alternative sequence966 – 100641Missing in isoform b.
VSP_035037

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified September 2, 2008. Version 2.
Checksum: BEFC03296E74DB1A

FASTA1,006113,614
        10         20         30         40         50         60 
MTVTSSAAID IGGGGGGRRS DRLDSDRTSE DMSFIASPAN ESFQIGASFV DVQNESSGSI 

        70         80         90        100        110        120 
DTATATLHEL NYTFGMPPVT EESENMPQNY ETVVELLPGE ERAPINKLTE FPIEGGSLFV 

       130        140        150        160        170        180 
TNFRIVVILK DKEVEEALRF LVFPLQDIEQ IDLAIPAFIH LSLKIGRMFT ICFKTAEDAA 

       190        200        210        220        230        240 
LVHKILYTAF QRLNRPISSI YTSRPQDWTS KNTDNPMQSL NAFAWKFSEA VDELDRDGKL 

       250        260        270        280        290        300 
PSWLLRADSV AQEITHIDFN RLGMSEHFQI SSVNENFEVC PTYPEKIIVP KGITDDDIRK 

       310        320        330        340        350        360 
GAPYRSIGRF PAVIWRCRKT RAVLMRSSQP QVGILSWRNP TDEKIIEEAV KASRIEGEEK 

       370        380        390        400        410        420 
KQFIIMDARG YTSAFANRAR SGGFENTEYY QQAKLEFLGL PNIHAVRGSF NNVRTMLHNL 

       430        440        450        460        470        480 
GPNEQLLTSL QTTGWLLNLS NLLVNAANCA DHLSKGHSVL VHCSDGWDRT TQVTTLAKIM 

       490        500        510        520        530        540 
LDEYYRTVKG FEELIRRDWI AFGHKLYDRQ LVAFGNWGTS DERSPVFLQF LEAVRHLQRE 

       550        560        570        580        590        600 
QPTLFQFTHA YLIKLAKHAY SGLFGSFLFN SHKERREAME KCKGTLVDIW RFIGPHNEEY 

       610        620        630        640        650        660 
VNQSFDEHYT GAVKPVNVSV INLRVWHEVF ADEEEHYTQI FSPKEERPLS GCTTPMNTST 

       670        680        690        700        710        720 
STNLVKSKSS ESINSLNVDG SAKESSQQHP TCSTTPSDNT NSLPMSTSFI QQSLYQPKVR 

       730        740        750        760        770        780 
GVAAIDRDGV IRFEDDEQAM LRKKNKLRAE EIRRKDEKIE ELRRRAVLDT NKVSPGQRQS 

       790        800        810        820        830        840 
YSESDVETTG TLERVMSDVS MVDPVNELPH FKPNTTWEGE SGHCAYCKKE FNKLSVYVED 

       850        860        870        880        890        900 
RQHHCRNCGR VVCEDCSKNR FSVIEEGKSV QKRACDSCYD SMHETDLKLS SSSTTTTSSS 

       910        920        930        940        950        960 
TKIENDSNVP GLDNNSDNVS ENVSENAIPD IIVEEKEAED PIKEAESPSK ETKCPKTLRN 

       970        980        990       1000 
FISFSPKSSM RKNKVHSRDP LKSIDEGSSS QQAESDDVLD VNEQPL 

« Hide

Isoform b [UniParc].

Checksum: 7EAC08FCD831B9C9
Show »

FASTA961108,189

References

« Hide 'large scale' references
[1]"Characterization of the myotubularin dual specificity phosphatase gene family from yeast to human."
Laporte J., Blondeau F., Buj-Bello A., Tentler D., Kretz C., Dahl N., Mandel J.-L.
Hum. Mol. Genet. 7:1703-1712(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
[2]"Characterization and functional studies of a FYVE domain-containing phosphatase in C. elegans."
Ma J., Zeng F., Ho W.T., Teng L., Li Q., Fu X., Zhao Z.J.
J. Cell. Biochem. 104:1843-1852(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), FUNCTION, PHOSPHATIDYLINOSITOL 3-PHOSPHATE-BINDING, ENZYME REGULATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
[3]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF031519 mRNA. Translation: AAC78763.1. Frameshift.
DQ988041 mRNA. Translation: ABK59970.1.
Z49072 Genomic DNA. Translation: CAA88884.2.
Z49072 Genomic DNA. Translation: CAB61017.2.
PIRT25215.
RefSeqNP_001022794.2. NM_001027623.5.
NP_497766.3. NM_065365.6.
UniGeneCel.18074.

3D structure databases

ProteinModelPortalQ22712.
SMRQ22712. Positions 91-635, 841-887.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid40728. 3 interactions.

Proteomic databases

PaxDbQ22712.
PRIDEQ22712.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaT24A11.1a; T24A11.1a; T24A11.1. [Q22712-1]
GeneID175490.
KEGGcel:CELE_T24A11.1.

Organism-specific databases

CTD175490.
WormBaseT24A11.1a; CE28087; WBGene00003476; mtm-3.
T24A11.1b; CE42568; WBGene00003476; mtm-3.

Phylogenomic databases

eggNOGNOG322789.
HOGENOMHOG000018814.
InParanoidQ22712.
OMAAKHAYSG.
OrthoDBEOG7PVWNJ.
PhylomeDBQ22712.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR010569. Myotubularin-like_Pase_dom.
IPR016130. Tyr_Pase_AS.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF01363. FYVE. 1 hit.
PF06602. Myotub-related. 1 hit.
[Graphical view]
SMARTSM00064. FYVE. 1 hit.
[Graphical view]
SUPFAMSSF57903. SSF57903. 1 hit.
PROSITEPS51339. PPASE_MYOTUBULARIN. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio888376.

Entry information

Entry nameMTMR3_CAEEL
AccessionPrimary (citable) accession number: Q22712
Secondary accession number(s): A8NBE6, Q9U360, Q9XTK4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 2, 2008
Last modified: April 16, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase