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Protein

Myotubularin-related protein 3

Gene

mtm-3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Preferentially dephosphorylates phosphatidylinositol 3-phosphate (PI3P), and has some activity towards phosphatidylinositol 3,5-bisphosphate (PI35P). May also dephosphorylate proteins phosphorylated on Ser, Thr, and Tyr residues (By similarity). Thought to have a role in maintenance of muscle function. Involved in locomotion and lifespan determination.By similarity1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotationBy similarity
1-phosphatidyl-1D-myo-inositol 3-phosphate + H2O = 1-phosphatidyl-1D-myo-inositol + phosphate.1 Publication
1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate.1 Publication

Enzyme regulationi

Inhibited by sodium vanadate and peroxide.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei463Phosphocysteine intermediatePROSITE-ProRule annotation1
Binding sitei509SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri818 – 883FYVE-typePROSITE-ProRule annotationAdd BLAST66

GO - Molecular functioni

GO - Biological processi

  • determination of adult lifespan Source: WormBase
  • inositol phosphate dephosphorylation Source: WormBase
  • locomotion Source: WormBase
  • negative regulation of engulfment of apoptotic cell Source: WormBase
  • phosphatidylinositol dephosphorylation Source: UniProtKB
  • positive regulation of endocytosis Source: WormBase
  • regulation of oviposition Source: WormBase

Keywordsi

Molecular functionDevelopmental protein, Hydrolase
Biological processLipid metabolism
LigandLipid-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-CEL-1632852 Macroautophagy
R-CEL-1660499 Synthesis of PIPs at the plasma membrane
R-CEL-1660516 Synthesis of PIPs at the early endosome membrane
R-CEL-1660517 Synthesis of PIPs at the late endosome membrane
R-CEL-2173788 Downregulation of TGF-beta receptor signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Myotubularin-related protein 3 (EC:3.1.3.48By similarity)
Short name:
ceMTM3
Alternative name(s):
Myotubularin homologous protein 1
Short name:
ceMTMH1
Phosphatidylinositol-3,5-bisphosphate 3-phosphatase (EC:3.1.3.951 Publication)
Phosphatidylinositol-3-phosphate phosphatase (EC:3.1.3.641 Publication)
Gene namesi
Name:mtm-3
ORF Names:T24A11.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiT24A11.1a ; CE28087 ; WBGene00003476 ; mtm-3
T24A11.1b ; CE42568 ; WBGene00003476 ; mtm-3

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Worms exhibit a 2-fold increase in PI3P, sluggish body movement with progressive deterioration and defects in gestation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000949471 – 1006Myotubularin-related protein 3Add BLAST1006

Proteomic databases

EPDiQ22712
PaxDbiQ22712
PeptideAtlasiQ22712
PRIDEiQ22712

Expressioni

Tissue specificityi

Expressed in the body wall muscle, intestine and in eggs (PubMed:18393358). Expressed in head neurons (PubMed:12788949).2 Publications

Developmental stagei

Expressed most strongly in the egg with expression present in L1 but reducing through to L3 where it is very weak. No expression was found in L4. Strong expression is also seen in egg-laying adults and post-reproductive adults.1 Publication

Gene expression databases

BgeeiWBGene00003476

Interactioni

Protein-protein interaction databases

BioGridi40728, 3 interactors
STRINGi6239.T24A11.1a

Structurei

3D structure databases

ProteinModelPortaliQ22712
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini224 – 630Myotubularin phosphatasePROSITE-ProRule annotationAdd BLAST407

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni377 – 380Substrate bindingBy similarity4
Regioni402 – 403Substrate bindingBy similarity2
Regioni463 – 469Substrate bindingBy similarity7

Domaini

Interacts with phosphatidylinositol 3-phosphate (PI3P) via the FYVE-type domain.1 Publication

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri818 – 883FYVE-typePROSITE-ProRule annotationAdd BLAST66

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410INM7 Eukaryota
ENOG410YU6P LUCA
GeneTreeiENSGT00760000118832
HOGENOMiHOG000018814
InParanoidiQ22712
KOiK18082
OMAiFENTEYY
OrthoDBiEOG091G03HG
PhylomeDBiQ22712

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR010569 Myotubularin-like_Pase_dom
IPR030564 Myotubularin_fam
IPR029021 Prot-tyrosine_phosphatase-like
IPR016130 Tyr_Pase_AS
IPR003595 Tyr_Pase_cat
IPR000306 Znf_FYVE
IPR017455 Znf_FYVE-rel
IPR011011 Znf_FYVE_PHD
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR10807 PTHR10807, 1 hit
PfamiView protein in Pfam
PF01363 FYVE, 1 hit
PF06602 Myotub-related, 1 hit
SMARTiView protein in SMART
SM00064 FYVE, 1 hit
SM00404 PTPc_motif, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
SSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS51339 PPASE_MYOTUBULARIN, 1 hit
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50178 ZF_FYVE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q22712-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTVTSSAAID IGGGGGGRRS DRLDSDRTSE DMSFIASPAN ESFQIGASFV
60 70 80 90 100
DVQNESSGSI DTATATLHEL NYTFGMPPVT EESENMPQNY ETVVELLPGE
110 120 130 140 150
ERAPINKLTE FPIEGGSLFV TNFRIVVILK DKEVEEALRF LVFPLQDIEQ
160 170 180 190 200
IDLAIPAFIH LSLKIGRMFT ICFKTAEDAA LVHKILYTAF QRLNRPISSI
210 220 230 240 250
YTSRPQDWTS KNTDNPMQSL NAFAWKFSEA VDELDRDGKL PSWLLRADSV
260 270 280 290 300
AQEITHIDFN RLGMSEHFQI SSVNENFEVC PTYPEKIIVP KGITDDDIRK
310 320 330 340 350
GAPYRSIGRF PAVIWRCRKT RAVLMRSSQP QVGILSWRNP TDEKIIEEAV
360 370 380 390 400
KASRIEGEEK KQFIIMDARG YTSAFANRAR SGGFENTEYY QQAKLEFLGL
410 420 430 440 450
PNIHAVRGSF NNVRTMLHNL GPNEQLLTSL QTTGWLLNLS NLLVNAANCA
460 470 480 490 500
DHLSKGHSVL VHCSDGWDRT TQVTTLAKIM LDEYYRTVKG FEELIRRDWI
510 520 530 540 550
AFGHKLYDRQ LVAFGNWGTS DERSPVFLQF LEAVRHLQRE QPTLFQFTHA
560 570 580 590 600
YLIKLAKHAY SGLFGSFLFN SHKERREAME KCKGTLVDIW RFIGPHNEEY
610 620 630 640 650
VNQSFDEHYT GAVKPVNVSV INLRVWHEVF ADEEEHYTQI FSPKEERPLS
660 670 680 690 700
GCTTPMNTST STNLVKSKSS ESINSLNVDG SAKESSQQHP TCSTTPSDNT
710 720 730 740 750
NSLPMSTSFI QQSLYQPKVR GVAAIDRDGV IRFEDDEQAM LRKKNKLRAE
760 770 780 790 800
EIRRKDEKIE ELRRRAVLDT NKVSPGQRQS YSESDVETTG TLERVMSDVS
810 820 830 840 850
MVDPVNELPH FKPNTTWEGE SGHCAYCKKE FNKLSVYVED RQHHCRNCGR
860 870 880 890 900
VVCEDCSKNR FSVIEEGKSV QKRACDSCYD SMHETDLKLS SSSTTTTSSS
910 920 930 940 950
TKIENDSNVP GLDNNSDNVS ENVSENAIPD IIVEEKEAED PIKEAESPSK
960 970 980 990 1000
ETKCPKTLRN FISFSPKSSM RKNKVHSRDP LKSIDEGSSS QQAESDDVLD

VNEQPL
Length:1,006
Mass (Da):113,614
Last modified:September 2, 2008 - v2
Checksum:iBEFC03296E74DB1A
GO
Isoform b (identifier: Q22712-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     41-44: Missing.
     891-965: SSSTTTTSSS...KTLRNFISFS → RATSLSEMSS...RHSSTQAVKG
     966-1006: Missing.

Show »
Length:961
Mass (Da):108,189
Checksum:i7EAC08FCD831B9C9
GO

Sequence cautioni

The sequence AAC78763 differs from that shown. Reason: Frameshift at position 913.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03503541 – 44Missing in isoform b. 1 Publication4
Alternative sequenceiVSP_035036891 – 965SSSTT…FISFS → RATSLSEMSSFDSFGPSSPP ASSTSSSSLNMLASMSPTRP QPIPISCKSSSNSISSPRPS PGEFSRHSSTQAVKG in isoform b. 1 PublicationAdd BLAST75
Alternative sequenceiVSP_035037966 – 1006Missing in isoform b. 1 PublicationAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031519 mRNA Translation: AAC78763.1 Frameshift.
DQ988041 mRNA Translation: ABK59970.1
Z49072 Genomic DNA Translation: CAA88884.2
Z49072 Genomic DNA Translation: CAB61017.2
PIRiT25215
RefSeqiNP_001022794.2, NM_001027623.5 [Q22712-1]
NP_497766.3, NM_065365.6 [Q22712-2]
UniGeneiCel.18074

Genome annotation databases

EnsemblMetazoaiT24A11.1a; T24A11.1a; WBGene00003476 [Q22712-1]
GeneIDi175490
KEGGicel:CELE_T24A11.1
UCSCiT24A11.1b c. elegans

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMTMR3_CAEEL
AccessioniPrimary (citable) accession number: Q22712
Secondary accession number(s): A8NBE6, Q9U360, Q9XTK4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 2, 2008
Last modified: May 23, 2018
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families
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