ID   NAS26_CAEEL             Reviewed;         414 AA.
AC   Q22710;
DT   04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 4.
DT   16-JUN-2009, entry version 57.
DE   RecName: Full=Zinc metalloproteinase nas-26;
DE            EC=3.4.24.21;
DE   AltName: Full=Nematode astacin 26;
DE   AltName: Full=Tollish protein 1;
DE   Flags: Precursor;
GN   Name=toh-1; Synonyms=nas-26; ORFNames=T24A11.3;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Nematoda; Chromadorea; Rhabditida; Rhabditoidea;
OC   Rhabditidae; Peloderinae; Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   MEDLINE=99069613; PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for
RT   investigating biology.";
RL   Science 282:2012-2018(1998).
RN   [2]
RP   IDENTIFICATION.
RA   Finelli A.L., Savage C., Cho S.H., Padgett R.W.;
RT   "Tollish genes in C. elegans.";
RL   (er) Worm Breeder's Gazette 14(2):46(1996).
RN   [3]
RP   IDENTIFICATION, AND NOMENCLATURE.
RX   PubMed=14653817; DOI=10.1046/j.1432-1033.2003.03891.x;
RA   Moehrlen F., Hutter H., Zwilling R.;
RT   "The astacin protein family in Caenorhabditis elegans.";
RL   Eur. J. Biochem. 270:4909-4920(2003).
CC   -!- FUNCTION: Probable metalloprotease (By similarity).
CC   -!- CATALYTIC ACTIVITY: Hydrolysis of peptide bonds in substrates
CC       containing five or more amino acids, preferentially with Ala in
CC       P1', and Pro in P2'.
CC   -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC   -!- SUBCELLULAR LOCATION: Secreted (Potential).
CC   -!- SIMILARITY: Belongs to the peptidase M12A family.
CC   -!- SIMILARITY: Contains 1 CUB domain.
CC   -!- SIMILARITY: Contains 1 EGF-like domain.
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DR   EMBL; Z49072; CAA88882.4; -; Genomic_DNA.
DR   PIR; T25213; T25213.
DR   RefSeq; NP_497769.3; -.
DR   UniGene; Cel.22628; -.
DR   HSSP; P07584; 1AST.
DR   Ensembl; T24A11.3; Caenorhabditis elegans.
DR   GeneID; 175491; -.
DR   KEGG; cel:T24A11.3; -.
DR   NMPDR; fig|6239.3.peg.9220; -.
DR   WormBase; WBGene00006591; toh-1.
DR   WormPep; T24A11.3; CE35909.
DR   OMA; Q22710; AKVINDI.
DR   BRENDA; 3.4.24.21; 672.
DR   NextBio; 888382; -.
DR   ArrayExpress; Q22710; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR   InterPro; IPR000859; CUB.
DR   InterPro; IPR013032; EGF-like_reg_CS.
DR   InterPro; IPR006025; Pept_M_Zn_BS.
DR   InterPro; IPR006026; Peptidase_M.
DR   InterPro; IPR001506; Peptidase_M12A.
DR   InterPro; IPR017050; Peptidase_M12A_nem.
DR   Gene3D; G3DSA:2.60.120.290; CUB; 1.
DR   Pfam; PF01400; Astacin; 1.
DR   Pfam; PF00431; CUB; 1.
DR   PIRSF; PIRSF036365; Astacin_nematoda; 1.
DR   PRINTS; PR00480; ASTACIN.
DR   SMART; SM00042; CUB; 1.
DR   SMART; SM00235; ZnMc; 1.
DR   PROSITE; PS01180; CUB; 1.
DR   PROSITE; PS00022; EGF_1; 1.
DR   PROSITE; PS01186; EGF_2; FALSE_NEG.
DR   PROSITE; PS50026; EGF_3; FALSE_NEG.
DR   PROSITE; PS00142; ZINC_PROTEASE; FALSE_NEG.
PE   2: Evidence at transcript level;
KW   Complete proteome; Disulfide bond; EGF-like domain; Glycoprotein;
KW   Hydrolase; Metal-binding; Metalloprotease; Protease; Secreted; Signal;
KW   Zinc.
FT   SIGNAL        1     20       Potential.
FT   CHAIN        21    414       Zinc metalloproteinase nas-26.
FT                                /FTId=PRO_0000028930.
FT   DOMAIN      251    307       EGF-like.
FT   DOMAIN      308    414       CUB.
FT   ACT_SITE    155    155       By similarity.
FT   METAL       154    154       Zinc; catalytic (By similarity).
FT   METAL       158    158       Zinc; catalytic (By similarity).
FT   METAL       164    164       Zinc; catalytic (By similarity).
FT   CARBOHYD     24     24       N-linked (GlcNAc...) (Potential).
FT   DISULFID    263    275       By similarity.
FT   DISULFID    267    286       By similarity.
FT   DISULFID    289    300       By similarity.
FT   DISULFID    308    331       By similarity.
FT   DISULFID    358    378       By similarity.
SQ   SEQUENCE   414 AA;  47140 MW;  B8B381B421239D1B CRC64;
     MTSSLVLILA PLALVAIGEA AFGNSSKIFE IPGLEVMASD KYPHFTTIET VSRTKVHRHR
     REVIAGQIYD WNSYEIPFQI WGGDYNFQSL IRRGIRMWED STCLRFKENQ QSRDAIRYVL
     EKGDSCFTEY IGRNGGHQDI IIGSECAEEY VVAHETGHAL GFWHTHQRPD RDRHISINWK
     NVMEEATASF MPFRSMLQAF GIRQVSPRRV PYDYGSLMHY HAVAHAVKVS DFTIVPKELK
     YVTTMGTEKM AFLDAKVIND IYCPNACQGR NHLNCLAGGY PDPNNCNVCR CPEGLGGPDC
     GRLQPSPCGG EIHASDQWQT LSSPSGRDVH CYWRISVPEG SRVRFRLSDG EFPCSYGCQS
     YVEIKHKLDV RLTGFRSCCY RPKEDTVSES NQIFVIYHPN GRTARFSLRF RRQA
//