Reviewed, UniProtKB/Swiss-Prot Q22710 (NAS26_CAEEL)

Names and origin

Protein names

Recommended name:
    Zinc metalloproteinase nas-26
    EC=3.4.24.21
Alternative name(s):
    Nematode astacin 26
    Tollish protein 1

Gene names

Name:	toh-1
Synonyms:	nas-26
ORF Names:	T24A11.3

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable metalloprotease By similarity.
Catalytic activity	Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the peptidase M12A family. Contains 1 CUB domain. Contains 1 EGF-like domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	EGF-like domain Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 414	394	Zinc metalloproteinase nas-26		PRO_0000028930
Regions
	Domain	251 – 307	57	EGF-like
	Domain	308 – 414	107	CUB
Sites
	Active site	155	1	By similarity
	Metal binding	154	1	Zinc; catalytic By similarity
	Metal binding	158	1	Zinc; catalytic By similarity
	Metal binding	164	1	Zinc; catalytic By similarity
Amino acid modifications
	Glycosylation	24	1	N-linked (GlcNAc...) Potential
	Disulfide bond	263 ↔ 275		By similarity
	Disulfide bond	267 ↔ 286		By similarity
	Disulfide bond	289 ↔ 300		By similarity
	Disulfide bond	308 ↔ 331		By similarity
	Disulfide bond	358 ↔ 378		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q22710-1 [UniParc]. Last modified July 5, 2004. Version 4. Checksum: B8B381B421239D1B	FASTA	414	47,140
	10 20 30 40 50 60 MTSSLVLILA PLALVAIGEA AFGNSSKIFE IPGLEVMASD KYPHFTTIET VSRTKVHRHR 70 80 90 100 110 120 REVIAGQIYD WNSYEIPFQI WGGDYNFQSL IRRGIRMWED STCLRFKENQ QSRDAIRYVL 130 140 150 160 170 180 EKGDSCFTEY IGRNGGHQDI IIGSECAEEY VVAHETGHAL GFWHTHQRPD RDRHISINWK 190 200 210 220 230 240 NVMEEATASF MPFRSMLQAF GIRQVSPRRV PYDYGSLMHY HAVAHAVKVS DFTIVPKELK 250 260 270 280 290 300 YVTTMGTEKM AFLDAKVIND IYCPNACQGR NHLNCLAGGY PDPNNCNVCR CPEGLGGPDC 310 320 330 340 350 360 GRLQPSPCGG EIHASDQWQT LSSPSGRDVH CYWRISVPEG SRVRFRLSDG EFPCSYGCQS 370 380 390 400 410 YVEIKHKLDV RLTGFRSCCY RPKEDTVSES NQIFVIYHPN GRTARFSLRF RRQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Tollish genes in C. elegans." Finelli A.L., Savage C., Cho S.H., Padgett R.W. Worm Breeder's Gazette 14(2):46(1996) Cited for: IDENTIFICATION.
[3]	"The astacin protein family in Caenorhabditis elegans." Moehrlen F., Hutter H., Zwilling R. Eur. J. Biochem. 270:4909-4920(2003) [PubMed: 14653817] [Abstract] Cited for: IDENTIFICATION, NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49072 Genomic DNA. Translation: CAA88882.4.
PIR	T25213.
RefSeq	NP_497769.3.
UniGene	Cel.22628
3D structure databases
HSSP	HSSP built from PDB template 1AST based on UniProtKB P07584.
ModBase	Search...
Genome annotation databases
Ensembl	T24A11.3. Caenorhabditis elegans. [Contig view]
GeneID	175491.
KEGG	cel:T24A11.3.
NMPDR	fig|6239.3.peg.9220.
Organism-specific databases
WormBase	WBGene00006591. toh-1.
WormPep	T24A11.3. CE35909. [WorfDB]
Phylogenomic databases
OMA	Q22710. AKVINDI.
Enzyme and pathway databases
BRENDA	3.4.24.21. 672.
Gene expression databases
ArrayExpress	Q22710.
Family and domain databases
InterPro	IPR000859. CUB. IPR013032. EGF-like_reg_CS. IPR006025. Pept_M_Zn_BS. IPR006026. Peptidase_M. IPR001506. Peptidase_M12A. IPR017050. Peptidase_M12A_nem. [Graphical view]
Gene3D	G3DSA:2.60.120.290. CUB. 1 hit.
Pfam	PF01400. Astacin. 1 hit. PF00431. CUB. 1 hit. [Graphical view]
PIRSF	PIRSF036365. Astacin_nematoda. 1 hit.
PRINTS	PR00480. ASTACIN.
SMART	SM00042. CUB. 1 hit. SM00235. ZnMc. 1 hit. [Graphical view]
PROSITE	PS01180. CUB. 1 hit. PS00022. EGF_1. 1 hit. PS01186. EGF_2. False negative. PS50026. EGF_3. False negative. PS00142. ZINC_PROTEASE. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	888382.

Entry information

Entry name

NAS26_CAEEL

Accession

Primary (citable) accession number: Q22710

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 57 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project