ID   NDUS8_CAEEL             Reviewed;         212 AA.
AC   Q22619;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 76.
DE   RecName: Full=Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial;
DE            EC=1.6.5.3;
DE            EC=1.6.99.3;
DE   AltName: Full=NADH-ubiquinone oxidoreductase 23 kDa subunit;
DE   AltName: Full=Complex I-23kD;
DE            Short=CI-23kD;
DE   Flags: Precursor;
GN   ORFNames=T20H4.5;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Nematoda; Chromadorea; Rhabditida; Rhabditoidea;
OC   Rhabditidae; Peloderinae; Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Bristol N2;
RX   MEDLINE=99377169; PubMed=10446229; DOI=10.1093/nar/27.17.3424;
RA   Hough R.F., Lingam A.T., Bass B.L.;
RT   "Caenorhabditis elegans mRNAs that encode a protein similar to ADARs
RT   derive from an operon containing six genes.";
RL   Nucleic Acids Res. 27:3424-3432(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   MEDLINE=99069613; PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for
RT   investigating biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory
CC       chain NADH dehydrogenase (Complex I) that is believed to belong to
CC       the minimal assembly required for catalysis. Complex I functions
CC       in the transfer of electrons from NADH to the respiratory chain.
CC       The immediate electron acceptor for the enzyme is believed to be
CC       ubiquinone (By similarity).
CC   -!- CATALYTIC ACTIVITY: NADH + ubiquinone = NAD(+) + ubiquinol.
CC   -!- CATALYTIC ACTIVITY: NADH + acceptor = NAD(+) + reduced acceptor.
CC   -!- COFACTOR: Binds 2 4Fe-4S clusters per subunit (By similarity).
CC   -!- SUBUNIT: Complex I is composed of 45 different subunits This is a
CC       component of the iron-sulfur (IP) fragment of the enzyme (By
CC       similarity).
CC   -!- SUBCELLULAR LOCATION: Mitochondrion (By similarity).
CC   -!- SIMILARITY: Belongs to the complex I 23 kDa subunit family.
CC   -!- SIMILARITY: Contains 2 4Fe-4S ferredoxin-type domains.
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DR   EMBL; AF140272; AAD34863.1; -; mRNA.
DR   EMBL; U00037; AAA50662.1; -; Genomic_DNA.
DR   PIR; T16914; T16914.
DR   RefSeq; NP_498595.1; -.
DR   UniGene; Cel.34232; -.
DR   HSSP; P00198; 2FDN.
DR   DIP; DIP:24953N; -.
DR   IntAct; Q22619; 3.
DR   Ensembl; T20H4.5; Caenorhabditis elegans.
DR   GeneID; 176023; -.
DR   KEGG; cel:T20H4.5; -.
DR   NMPDR; fig|6239.3.peg.10480; -.
DR   WormBase; WBGene00020636; T20H4.5.
DR   WormPep; T20H4.5; CE00832.
DR   OMA; Q22619; DFFRINF.
DR   BRENDA; 1.6.5.3; 672.
DR   BRENDA; 1.6.99.3; 672.
DR   NextBio; 890788; -.
DR   ArrayExpress; Q22619; -.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respiratory chain; IEA:UniProtKB-KW.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:EC.
DR   GO; GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
DR   GO; GO:0009792; P:embryonic development ending in birth or eg...; IMP:WormBase.
DR   GO; GO:0040007; P:growth; IMP:WormBase.
DR   GO; GO:0002119; P:nematode larval development; IMP:WormBase.
DR   GO; GO:0040010; P:positive regulation of growth rate; IMP:WormBase.
DR   GO; GO:0006810; P:transport; IEA:UniProtKB-KW.
DR   InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR   InterPro; IPR001450; 4Fe4S_Fe_S_bd_subgr.
DR   InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR   InterPro; IPR010226; NADH_quinone_OxRdtase_chainI.
DR   Pfam; PF00037; Fer4; 2.
DR   TIGRFAMs; TIGR01971; NuoI; 1.
DR   PROSITE; PS00198; 4FE4S_FER_1; 2.
DR   PROSITE; PS51379; 4FE4S_FER_2; 2.
PE   2: Evidence at transcript level;
KW   4Fe-4S; Complete proteome; Electron transport; Iron; Iron-sulfur;
KW   Metal-binding; Mitochondrion; NAD; Oxidoreductase; Repeat;
KW   Respiratory chain; Transit peptide; Transport; Ubiquinone.
FT   TRANSIT       1      ?       Mitochondrion (By similarity).
FT   CHAIN         ?    212       Probable NADH dehydrogenase [ubiquinone]
FT                                iron-sulfur protein 8, mitochondrial.
FT                                /FTId=PRO_0000020015.
FT   DOMAIN      104    133       4Fe-4S ferredoxin-type 1.
FT   DOMAIN      143    172       4Fe-4S ferredoxin-type 2.
FT   METAL       113    113       Iron-sulfur 1 (4Fe-4S) (By similarity).
FT   METAL       116    116       Iron-sulfur 1 (4Fe-4S) (By similarity).
FT   METAL       119    119       Iron-sulfur 1 (4Fe-4S) (By similarity).
FT   METAL       123    123       Iron-sulfur 2 (4Fe-4S) (By similarity).
FT   METAL       152    152       Iron-sulfur 2 (4Fe-4S) (By similarity).
FT   METAL       155    155       Iron-sulfur 2 (4Fe-4S) (By similarity).
FT   METAL       158    158       Iron-sulfur 2 (4Fe-4S) (By similarity).
FT   METAL       162    162       Iron-sulfur 1 (4Fe-4S) (By similarity).
SQ   SEQUENCE   212 AA;  23870 MW;  F50C413D89E80AB7 CRC64;
     MAMKSVAVLT KGMFGRIPAQ LAVSSVPSNT IQKRSNYKFV GMPNETDGTL AGDLNYGLHN
     VFFTELFRGF GVMLGHVFME PATINYPFEK GPLSSRFRGE HALRRYPSGE ERCIACKLCE
     AICPAQAITI EAETRPDGSR RTTRYDIDMT KCIYCGLCQE ACPVDAIVEG PNFEYSTETH
     EELLYNKEKL LLNGDRWEPE LASNLQAEYL YR
//