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Q22619 (NDUS8_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

EC=1.6.5.3
EC=1.6.99.3
Alternative name(s):
Complex I-23kD
Short name=CI-23kD
NADH-ubiquinone oxidoreductase 23 kDa subunit
Gene names
ORF Names:T20H4.5
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length212 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. HAMAP-Rule MF_01351

Catalytic activity

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out). HAMAP-Rule MF_01351

NADH + acceptor = NAD+ + reduced acceptor. HAMAP-Rule MF_01351

Cofactor

Binds 2 4Fe-4S clusters per subunit By similarity. HAMAP-Rule MF_01351

Subunit structure

Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme By similarity.

Subcellular location

Mitochondrion By similarity HAMAP-Rule MF_01351.

Sequence similarities

Belongs to the complex I 23 kDa subunit family.

Contains 2 4Fe-4S ferredoxin-type domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion By similarity
Chain? – 212Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial HAMAP-Rule MF_01351PRO_0000020015

Regions

Domain104 – 133304Fe-4S ferredoxin-type 1
Domain143 – 172304Fe-4S ferredoxin-type 2

Sites

Metal binding1131Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1161Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1191Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1231Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1521Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1551Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1581Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1621Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q22619 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: F50C413D89E80AB7

FASTA21223,870
        10         20         30         40         50         60 
MAMKSVAVLT KGMFGRIPAQ LAVSSVPSNT IQKRSNYKFV GMPNETDGTL AGDLNYGLHN 

        70         80         90        100        110        120 
VFFTELFRGF GVMLGHVFME PATINYPFEK GPLSSRFRGE HALRRYPSGE ERCIACKLCE 

       130        140        150        160        170        180 
AICPAQAITI EAETRPDGSR RTTRYDIDMT KCIYCGLCQE ACPVDAIVEG PNFEYSTETH 

       190        200        210 
EELLYNKEKL LLNGDRWEPE LASNLQAEYL YR 

« Hide

References

« Hide 'large scale' references
[1]"Caenorhabditis elegans mRNAs that encode a protein similar to ADARs derive from an operon containing six genes."
Hough R.F., Lingam A.T., Bass B.L.
Nucleic Acids Res. 27:3424-3432(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Bristol N2.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF140272 mRNA. Translation: AAD34863.1.
FO081693 Genomic DNA. Translation: CCD73352.1.
PIRT16914.
RefSeqNP_498595.1. NM_066194.4.
UniGeneCel.34232.

3D structure databases

ProteinModelPortalQ22619.
SMRQ22619. Positions 86-193.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid41235. 5 interactions.
DIPDIP-24953N.
MINTMINT-1095873.
STRING6239.T20H4.5.

Proteomic databases

PaxDbQ22619.
PRIDEQ22619.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaT20H4.5; T20H4.5; WBGene00020636.
GeneID176023.
KEGGcel:CELE_T20H4.5.
UCSCT20H4.5. c. elegans.

Organism-specific databases

CTD176023.
WormBaseT20H4.5; CE00832; WBGene00020636.

Phylogenomic databases

eggNOGCOG1143.
GeneTreeENSGT00390000003049.
HOGENOMHOG000228289.
InParanoidQ22619.
KOK03941.
OMANKELSME.
OrthoDBEOG7J9VQT.
PhylomeDBQ22619.

Family and domain databases

HAMAPMF_01351. NDH1_NuoI.
InterProIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamPF00037. Fer4. 2 hits.
[Graphical view]
TIGRFAMsTIGR01971. NuoI. 1 hit.
PROSITEPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio890788.
PROQ22619.

Entry information

Entry nameNDUS8_CAEEL
AccessionPrimary (citable) accession number: Q22619
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase