Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial precursor

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Q22619 (NDUS8_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 111. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
EC=1.6.5.3
EC=1.6.99.3

Alternative name(s):
Complex I-23kD
Short name=CI-23kD
NADH-ubiquinone oxidoreductase 23 kDa subunit

Gene names

ORF Names:

T20H4.5

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.
Catalytic activity	NADH + ubiquinone = NAD⁺ + ubiquinol. NADH + acceptor = NAD⁺ + reduced acceptor.
Cofactor	Binds 2 4Fe-4S clusters per subunit By similarity.
Subunit structure	Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the complex I 23 kDa subunit family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Domain	Repeat Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial electron transport, NADH to ubiquinone Inferred from mutant phenotype PubMed 16920626. Source: WormBase response to drug Inferred from mutant phenotype PubMed 16920626. Source: WormBase
Cellular_component	mitochondrion Inferred from direct assay PubMed 16443191. Source: WormBase respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – ?

Mitochondrion By similarity

Chain

? – 212

Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

PRO_0000020015

Regions

Domain

104 – 133

4Fe-4S ferredoxin-type 1

Domain

143 – 172

4Fe-4S ferredoxin-type 2

Sites

Metal binding

113

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

116

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

119

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

123

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

152

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

155

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

158

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

162

Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q22619 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: F50C413D89E80AB7
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FASTA

212

23,870

        10         20         30         40         50         60 
MAMKSVAVLT KGMFGRIPAQ LAVSSVPSNT IQKRSNYKFV GMPNETDGTL AGDLNYGLHN 

        70         80         90        100        110        120 
VFFTELFRGF GVMLGHVFME PATINYPFEK GPLSSRFRGE HALRRYPSGE ERCIACKLCE 

       130        140        150        160        170        180 
AICPAQAITI EAETRPDGSR RTTRYDIDMT KCIYCGLCQE ACPVDAIVEG PNFEYSTETH 

       190        200        210 
EELLYNKEKL LLNGDRWEPE LASNLQAEYL YR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Caenorhabditis elegans mRNAs that encode a protein similar to ADARs derive from an operon containing six genes." Hough R.F., Lingam A.T., Bass B.L. Nucleic Acids Res. 27:3424-3432(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF140272 mRNA. Translation: AAD34863.1. FO081693 Genomic DNA. Translation: CCD73352.1.
PIR	T16914.
RefSeq	NP_498595.1. NM_066194.4.
UniGene	Cel.34232.
3D structure databases
ProteinModelPortal	Q22619.
SMR	Q22619. Positions 86-193.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-24953N.
MINT	MINT-1095873.
STRING	6239.T20H4.5.
Proteomic databases
PaxDb	Q22619.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	T20H4.5; T20H4.5; T20H4.5.
GeneID	176023.
KEGG	cel:CELE_T20H4.5.
UCSC	T20H4.5. c. elegans.
Organism-specific databases
CTD	176023.
WormBase	T20H4.5; CE00832; WBGene00020636.
Phylogenomic databases
eggNOG	COG1143.
GeneTree	ENSGT00390000003049.
HOGENOM	HOG000228289.
InParanoid	Q22619.
KO	K03941.
OMA	DFFRINF.
Family and domain databases
Gene3D	1.10.1060.10. 1 hit.
InterPro	IPR001450. 4Fe4S-bd_dom. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR012285. Fum_reductase_C. IPR010226. NADH_quinone_OxRdtase_chainI. [Graphical view]
Pfam	PF00037. Fer4. 2 hits. [Graphical view]
TIGRFAMs	TIGR01971. NuoI. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	890788.

Entry information

Entry name

NDUS8_CAEEL

Accession

Primary (citable) accession number: Q22619

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents