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Protein

Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Gene

T20H4.5

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi113Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi116Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi119Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi123Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi152Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi155Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi158Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi162Iron-sulfur 1 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

  • mitochondrial electron transport, NADH to ubiquinone Source: WormBase
  • response to drug Source: WormBase

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

ReactomeiR-CEL-6799198. Complex I biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-23kD
Short name:
CI-23kD
NADH-ubiquinone oxidoreductase 23 kDa subunit
Gene namesi
ORF Names:T20H4.5
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiT20H4.5; CE00832; WBGene00020636.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial respiratory chain complex I Source: WormBase
  • mitochondrion Source: WormBase

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000020015? – 212Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
Transit peptidei1 – ?MitochondrionBy similarity

Proteomic databases

EPDiQ22619.
PaxDbiQ22619.
PeptideAtlasiQ22619.
PRIDEiQ22619.

Expressioni

Gene expression databases

BgeeiWBGene00020636.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme.By similarity

Protein-protein interaction databases

BioGridi41235. 5 interactors.
DIPiDIP-24953N.
MINTiMINT-1095873.
STRINGi6239.T20H4.5.

Structurei

3D structure databases

ProteinModelPortaliQ22619.
SMRiQ22619.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini104 – 1334Fe-4S ferredoxin-type 1Add BLAST30
Domaini143 – 1724Fe-4S ferredoxin-type 2Add BLAST30

Sequence similaritiesi

Belongs to the complex I 23 kDa subunit family.Curated

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG3256. Eukaryota.
COG1143. LUCA.
GeneTreeiENSGT00390000003049.
HOGENOMiHOG000228289.
InParanoidiQ22619.
KOiK03941.
OMAiSMSEPAT.
OrthoDBiEOG091G0OQD.
PhylomeDBiQ22619.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI. 1 hit.
InterProiView protein in InterPro
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
PfamiView protein in Pfam
PF12838. Fer4_7. 1 hit.
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiView protein in PROSITE
PS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q22619-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMKSVAVLT KGMFGRIPAQ LAVSSVPSNT IQKRSNYKFV GMPNETDGTL
60 70 80 90 100
AGDLNYGLHN VFFTELFRGF GVMLGHVFME PATINYPFEK GPLSSRFRGE
110 120 130 140 150
HALRRYPSGE ERCIACKLCE AICPAQAITI EAETRPDGSR RTTRYDIDMT
160 170 180 190 200
KCIYCGLCQE ACPVDAIVEG PNFEYSTETH EELLYNKEKL LLNGDRWEPE
210
LASNLQAEYL YR
Length:212
Mass (Da):23,870
Last modified:November 1, 1996 - v1
Checksum:iF50C413D89E80AB7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF140272 mRNA. Translation: AAD34863.1.
FO081693 Genomic DNA. Translation: CCD73352.1.
PIRiT16914.
RefSeqiNP_498595.1. NM_066194.4.
UniGeneiCel.34232.

Genome annotation databases

EnsemblMetazoaiT20H4.5; T20H4.5; WBGene00020636.
GeneIDi176023.
KEGGicel:CELE_T20H4.5.
UCSCiT20H4.5. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF140272 mRNA. Translation: AAD34863.1.
FO081693 Genomic DNA. Translation: CCD73352.1.
PIRiT16914.
RefSeqiNP_498595.1. NM_066194.4.
UniGeneiCel.34232.

3D structure databases

ProteinModelPortaliQ22619.
SMRiQ22619.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41235. 5 interactors.
DIPiDIP-24953N.
MINTiMINT-1095873.
STRINGi6239.T20H4.5.

Proteomic databases

EPDiQ22619.
PaxDbiQ22619.
PeptideAtlasiQ22619.
PRIDEiQ22619.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT20H4.5; T20H4.5; WBGene00020636.
GeneIDi176023.
KEGGicel:CELE_T20H4.5.
UCSCiT20H4.5. c. elegans.

Organism-specific databases

CTDi176023.
WormBaseiT20H4.5; CE00832; WBGene00020636.

Phylogenomic databases

eggNOGiKOG3256. Eukaryota.
COG1143. LUCA.
GeneTreeiENSGT00390000003049.
HOGENOMiHOG000228289.
InParanoidiQ22619.
KOiK03941.
OMAiSMSEPAT.
OrthoDBiEOG091G0OQD.
PhylomeDBiQ22619.

Enzyme and pathway databases

ReactomeiR-CEL-6799198. Complex I biogenesis.

Miscellaneous databases

PROiQ22619.

Gene expression databases

BgeeiWBGene00020636.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI. 1 hit.
InterProiView protein in InterPro
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
PfamiView protein in Pfam
PF12838. Fer4_7. 1 hit.
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiView protein in PROSITE
PS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiNDUS8_CAEEL
AccessioniPrimary (citable) accession number: Q22619
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: March 15, 2017
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.