Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial precursor

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Reviewed, UniProtKB/Swiss-Prot Q22619 (NDUS8_CAEEL)

Last modified June 16, 2009. Version 76. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
    EC=1.6.5.3
    EC=1.6.99.3
Alternative name(s):
    NADH-ubiquinone oxidoreductase 23 kDa subunit
    Complex I-23kD
      Short name=CI-23kD

Gene names

ORF Names:

T20H4.5

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

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Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.
Catalytic activity	NADH + ubiquinone = NAD⁺ + ubiquinol. NADH + acceptor = NAD⁺ + reduced acceptor.
Cofactor	Binds 2 4Fe-4S clusters per subunit By similarity.
Subunit structure	Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the complex I 23 kDa subunit family. Contains 2 4Fe-4S ferredoxin-type domains.

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Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Domain	Repeat Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – ?

Mitochondrion By similarity

Chain

? – 212

Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

PRO_0000020015

Regions

Domain

104 – 133

4Fe-4S ferredoxin-type 1

Domain

143 – 172

4Fe-4S ferredoxin-type 2

Sites

Metal binding

113

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

116

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

119

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

123

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

152

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

155

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

158

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

162

Iron-sulfur 1 (4Fe-4S) By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

Q22619-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: F50C413D89E80AB7
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FASTA

212

23,870

        10         20         30         40         50         60 
MAMKSVAVLT KGMFGRIPAQ LAVSSVPSNT IQKRSNYKFV GMPNETDGTL AGDLNYGLHN 

        70         80         90        100        110        120 
VFFTELFRGF GVMLGHVFME PATINYPFEK GPLSSRFRGE HALRRYPSGE ERCIACKLCE 

       130        140        150        160        170        180 
AICPAQAITI EAETRPDGSR RTTRYDIDMT KCIYCGLCQE ACPVDAIVEG PNFEYSTETH 

       190        200        210 
EELLYNKEKL LLNGDRWEPE LASNLQAEYL YR

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Caenorhabditis elegans mRNAs that encode a protein similar to ADARs derive from an operon containing six genes." Hough R.F., Lingam A.T., Bass B.L. Nucleic Acids Res. 27:3424-3432(1999) [PubMed: 10446229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

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Cross-references

Sequence databases
	AF140272 mRNA. Translation: AAD34863.1. U00037 Genomic DNA. Translation: AAA50662.1.
PIR	T16914.
RefSeq	NP_498595.1.
UniGene	Cel.34232
3D structure databases
HSSP	HSSP built from PDB template 2FDN based on UniProtKB P00198.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:24953N.
IntAct	Q22619. 3 interactions.
Genome annotation databases
Ensembl	T20H4.5. Caenorhabditis elegans. [Contig view]
GeneID	176023.
KEGG	cel:T20H4.5.
NMPDR	fig\|6239.3.peg.10480.
Organism-specific databases
WormBase	WBGene00020636. T20H4.5.
WormPep	T20H4.5. CE00832. [WorfDB]
Phylogenomic databases
OMA	Q22619. DFFRINF.
Enzyme and pathway databases
BRENDA	1.6.5.3. 672. 1.6.99.3. 672.
Gene expression databases
ArrayExpress	Q22619.
Family and domain databases
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR001450. 4Fe4S_Fe_S_bd_subgr. IPR017900. 4Fe4S_Fe_S_CS. IPR010226. NADH_quinone_OxRdtase_chainI. [Graphical view]
Pfam	PF00037. Fer4. 2 hits. [Graphical view]
TIGRFAMs	TIGR01971. NuoI. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	890788.

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Entry information

Entry name

NDUS8_CAEEL

Accession

Primary (citable) accession number: Q22619

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project

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Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents