Reviewed, UniProtKB/Swiss-Prot Q22619 (NDUS8_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
    EC=1.6.5.3
    EC=1.6.99.3
Alternative name(s):
    NADH-ubiquinone oxidoreductase 23 kDa subunit
    Complex I-23kD
      Short name=CI-23kD

Gene names

ORF Names:

T20H4.5

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.
Catalytic activity	NADH + ubiquinone = NAD(+) + ubiquinol. NADH + acceptor = NAD(+) + reduced acceptor.
Cofactor	Binds 2 4Fe-4S clusters per subunit By similarity.
Subunit structure	Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme By similarity.
Subcellular location	MitochondrionBy similarity.
Sequence similarities	Belongs to the complex I 23 kDa subunit family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Domain	Repeat Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion By similarity
	Chain	? – 212		Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial		PRO_0000020015
Regions
	Domain	104 – 133	30	4Fe-4S ferredoxin-type 1
	Domain	143 – 172	30	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	113	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	116	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	119	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	123	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	152	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	155	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	158	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	162	1	Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q22619-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F50C413D89E80AB7	FASTA	212	23,870
	10 20 30 40 50 60 MAMKSVAVLT KGMFGRIPAQ LAVSSVPSNT IQKRSNYKFV GMPNETDGTL AGDLNYGLHN 70 80 90 100 110 120 VFFTELFRGF GVMLGHVFME PATINYPFEK GPLSSRFRGE HALRRYPSGE ERCIACKLCE 130 140 150 160 170 180 AICPAQAITI EAETRPDGSR RTTRYDIDMT KCIYCGLCQE ACPVDAIVEG PNFEYSTETH 190 200 210 EELLYNKEKL LLNGDRWEPE LASNLQAEYL YR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Caenorhabditis elegans mRNAs that encode a protein similar to ADARs derive from an operon containing six genes." Hough R.F., Lingam A.T., Bass B.L. Nucleic Acids Res. 27:3424-3432(1999) [PubMed: 10446229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF140272 mRNA. Translation: AAD34863.1. U00037 Genomic DNA. Translation: AAA50662.1.
PIR	T16914.
RefSeq	NP_498595.1.
UniGene	Cel.34232
3D structure databases
HSSP	HSSP built from PDB template 2FDN based on UniProtKB P00198.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:24953N.
IntAct	Q22619.
Genome annotation databases
Ensembl	T20H4.5. Caenorhabditis elegans. [Contig view]
GeneID	176023.
KEGG	cel:T20H4.5.
NMPDR	fig|6239.3.peg.10480.
Organism-specific databases
WormBase	WBGene00020636. T20H4.5.
WormPep	T20H4.5. CE00832. [WorfDB]
Gene expression databases
ArrayExpress	Q22619.
Family and domain databases
InterPro	IPR001450. 4Fe4S_Fe_S_bd. IPR010226. NADH_quinone_OxRdtase_I. [Graphical view]
Pfam	PF00037. Fer4. 2 hits. [Graphical view]
PRINTS	PR00353. 4FE4SFRDOXIN.
TIGRFAMs	TIGR01971. NuoI. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	890788.

Entry information

Entry name

NDUS8_CAEEL

Accession

Primary (citable) accession number: Q22619

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project