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Q22619

- NDUS8_CAEEL

UniProt

Q22619 - NDUS8_CAEEL

Protein

Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Gene

T20H4.5

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.By similarity

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi113 – 1131Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi116 – 1161Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi119 – 1191Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi123 – 1231Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi152 – 1521Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi155 – 1551Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi158 – 1581Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi162 – 1621Iron-sulfur 1 (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

    GO - Biological processi

    1. mitochondrial electron transport, NADH to ubiquinone Source: WormBase
    2. response to drug Source: WormBase

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
    Alternative name(s):
    Complex I-23kD
    Short name:
    CI-23kD
    NADH-ubiquinone oxidoreductase 23 kDa subunit
    Gene namesi
    ORF Names:T20H4.5
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome III

    Organism-specific databases

    WormBaseiT20H4.5; CE00832; WBGene00020636.

    Subcellular locationi

    Mitochondrion By similarity

    GO - Cellular componenti

    1. mitochondrion Source: WormBase
    2. respiratory chain Source: UniProtKB-KW

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 212Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrialPRO_0000020015
    Transit peptidei1 – ?MitochondrionBy similarity

    Proteomic databases

    PaxDbiQ22619.
    PRIDEiQ22619.

    Interactioni

    Subunit structurei

    Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme.By similarity

    Protein-protein interaction databases

    BioGridi41235. 5 interactions.
    DIPiDIP-24953N.
    MINTiMINT-1095873.
    STRINGi6239.T20H4.5.

    Structurei

    3D structure databases

    ProteinModelPortaliQ22619.
    SMRiQ22619. Positions 86-193.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini104 – 133304Fe-4S ferredoxin-type 1Add
    BLAST
    Domaini143 – 172304Fe-4S ferredoxin-type 2Add
    BLAST

    Sequence similaritiesi

    Belongs to the complex I 23 kDa subunit family.Curated
    Contains 2 4Fe-4S ferredoxin-type domains.Curated

    Keywords - Domaini

    Repeat, Transit peptide

    Phylogenomic databases

    eggNOGiCOG1143.
    GeneTreeiENSGT00390000003049.
    HOGENOMiHOG000228289.
    InParanoidiQ22619.
    KOiK03941.
    OMAiNKELSME.
    OrthoDBiEOG7J9VQT.
    PhylomeDBiQ22619.

    Family and domain databases

    HAMAPiMF_01351. NDH1_NuoI.
    InterProiIPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR010226. NADH_quinone_OxRdtase_chainI.
    [Graphical view]
    PfamiPF00037. Fer4. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR01971. NuoI. 1 hit.
    PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q22619-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAMKSVAVLT KGMFGRIPAQ LAVSSVPSNT IQKRSNYKFV GMPNETDGTL    50
    AGDLNYGLHN VFFTELFRGF GVMLGHVFME PATINYPFEK GPLSSRFRGE 100
    HALRRYPSGE ERCIACKLCE AICPAQAITI EAETRPDGSR RTTRYDIDMT 150
    KCIYCGLCQE ACPVDAIVEG PNFEYSTETH EELLYNKEKL LLNGDRWEPE 200
    LASNLQAEYL YR 212
    Length:212
    Mass (Da):23,870
    Last modified:November 1, 1996 - v1
    Checksum:iF50C413D89E80AB7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF140272 mRNA. Translation: AAD34863.1.
    FO081693 Genomic DNA. Translation: CCD73352.1.
    PIRiT16914.
    RefSeqiNP_498595.1. NM_066194.4.
    UniGeneiCel.34232.

    Genome annotation databases

    EnsemblMetazoaiT20H4.5; T20H4.5; WBGene00020636.
    GeneIDi176023.
    KEGGicel:CELE_T20H4.5.
    UCSCiT20H4.5. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF140272 mRNA. Translation: AAD34863.1 .
    FO081693 Genomic DNA. Translation: CCD73352.1 .
    PIRi T16914.
    RefSeqi NP_498595.1. NM_066194.4.
    UniGenei Cel.34232.

    3D structure databases

    ProteinModelPortali Q22619.
    SMRi Q22619. Positions 86-193.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 41235. 5 interactions.
    DIPi DIP-24953N.
    MINTi MINT-1095873.
    STRINGi 6239.T20H4.5.

    Proteomic databases

    PaxDbi Q22619.
    PRIDEi Q22619.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai T20H4.5 ; T20H4.5 ; WBGene00020636 .
    GeneIDi 176023.
    KEGGi cel:CELE_T20H4.5.
    UCSCi T20H4.5. c. elegans.

    Organism-specific databases

    CTDi 176023.
    WormBasei T20H4.5 ; CE00832 ; WBGene00020636 .

    Phylogenomic databases

    eggNOGi COG1143.
    GeneTreei ENSGT00390000003049.
    HOGENOMi HOG000228289.
    InParanoidi Q22619.
    KOi K03941.
    OMAi NKELSME.
    OrthoDBi EOG7J9VQT.
    PhylomeDBi Q22619.

    Miscellaneous databases

    NextBioi 890788.
    PROi Q22619.

    Family and domain databases

    HAMAPi MF_01351. NDH1_NuoI.
    InterProi IPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR010226. NADH_quinone_OxRdtase_chainI.
    [Graphical view ]
    Pfami PF00037. Fer4. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR01971. NuoI. 1 hit.
    PROSITEi PS00198. 4FE4S_FER_1. 2 hits.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Caenorhabditis elegans mRNAs that encode a protein similar to ADARs derive from an operon containing six genes."
      Hough R.F., Lingam A.T., Bass B.L.
      Nucleic Acids Res. 27:3424-3432(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Bristol N2.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.

    Entry informationi

    Entry nameiNDUS8_CAEEL
    AccessioniPrimary (citable) accession number: Q22619
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3