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Protein

Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Gene

T20H4.5

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi113 – 1131Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi116 – 1161Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi119 – 1191Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi123 – 1231Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi152 – 1521Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi155 – 1551Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi158 – 1581Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi162 – 1621Iron-sulfur 1 (4Fe-4S)By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

GO - Biological processi

  1. mitochondrial electron transport, NADH to ubiquinone Source: WormBase
  2. response to drug Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-23kD
Short name:
CI-23kD
NADH-ubiquinone oxidoreductase 23 kDa subunit
Gene namesi
ORF Names:T20H4.5
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome III

Organism-specific databases

WormBaseiT20H4.5; CE00832; WBGene00020636.

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. mitochondrion Source: WormBase
  2. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 212Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrialPRO_0000020015
Transit peptidei1 – ?MitochondrionBy similarity

Proteomic databases

PaxDbiQ22619.
PRIDEiQ22619.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme.By similarity

Protein-protein interaction databases

BioGridi41235. 5 interactions.
DIPiDIP-24953N.
MINTiMINT-1095873.
STRINGi6239.T20H4.5.

Structurei

3D structure databases

ProteinModelPortaliQ22619.
SMRiQ22619. Positions 86-193.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini104 – 133304Fe-4S ferredoxin-type 1Add
BLAST
Domaini143 – 172304Fe-4S ferredoxin-type 2Add
BLAST

Sequence similaritiesi

Belongs to the complex I 23 kDa subunit family.Curated
Contains 2 4Fe-4S ferredoxin-type domains.Curated

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiCOG1143.
GeneTreeiENSGT00390000003049.
HOGENOMiHOG000228289.
InParanoidiQ22619.
KOiK03941.
OMAiFGHGFRK.
OrthoDBiEOG7J9VQT.
PhylomeDBiQ22619.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI.
InterProiIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF00037. Fer4. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q22619-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMKSVAVLT KGMFGRIPAQ LAVSSVPSNT IQKRSNYKFV GMPNETDGTL
60 70 80 90 100
AGDLNYGLHN VFFTELFRGF GVMLGHVFME PATINYPFEK GPLSSRFRGE
110 120 130 140 150
HALRRYPSGE ERCIACKLCE AICPAQAITI EAETRPDGSR RTTRYDIDMT
160 170 180 190 200
KCIYCGLCQE ACPVDAIVEG PNFEYSTETH EELLYNKEKL LLNGDRWEPE
210
LASNLQAEYL YR
Length:212
Mass (Da):23,870
Last modified:November 1, 1996 - v1
Checksum:iF50C413D89E80AB7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF140272 mRNA. Translation: AAD34863.1.
FO081693 Genomic DNA. Translation: CCD73352.1.
PIRiT16914.
RefSeqiNP_498595.1. NM_066194.4.
UniGeneiCel.34232.

Genome annotation databases

EnsemblMetazoaiT20H4.5; T20H4.5; WBGene00020636.
GeneIDi176023.
KEGGicel:CELE_T20H4.5.
UCSCiT20H4.5. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF140272 mRNA. Translation: AAD34863.1.
FO081693 Genomic DNA. Translation: CCD73352.1.
PIRiT16914.
RefSeqiNP_498595.1. NM_066194.4.
UniGeneiCel.34232.

3D structure databases

ProteinModelPortaliQ22619.
SMRiQ22619. Positions 86-193.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41235. 5 interactions.
DIPiDIP-24953N.
MINTiMINT-1095873.
STRINGi6239.T20H4.5.

Proteomic databases

PaxDbiQ22619.
PRIDEiQ22619.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT20H4.5; T20H4.5; WBGene00020636.
GeneIDi176023.
KEGGicel:CELE_T20H4.5.
UCSCiT20H4.5. c. elegans.

Organism-specific databases

CTDi176023.
WormBaseiT20H4.5; CE00832; WBGene00020636.

Phylogenomic databases

eggNOGiCOG1143.
GeneTreeiENSGT00390000003049.
HOGENOMiHOG000228289.
InParanoidiQ22619.
KOiK03941.
OMAiFGHGFRK.
OrthoDBiEOG7J9VQT.
PhylomeDBiQ22619.

Miscellaneous databases

NextBioi890788.
PROiQ22619.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI.
InterProiIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF00037. Fer4. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Caenorhabditis elegans mRNAs that encode a protein similar to ADARs derive from an operon containing six genes."
    Hough R.F., Lingam A.T., Bass B.L.
    Nucleic Acids Res. 27:3424-3432(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiNDUS8_CAEEL
AccessioniPrimary (citable) accession number: Q22619
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.