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Q22492

- HEXA_CAEEL

UniProt

Q22492 - HEXA_CAEEL

Protein

Beta-hexosaminidase A

Gene

hex-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines. Degrades chitotriose.1 Publication

    Catalytic activityi

    Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.1 Publication

    Kineticsi

    1. KM=1.1 mM for p-nitrophenyl-beta-N-acetylglucosaminide1 Publication

    pH dependencei

    Optimum pH is 5.5.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei325 – 3251Proton donorBy similarity

    GO - Molecular functioni

    1. beta-N-acetylhexosaminidase activity Source: UniProtKB
    2. hexosaminidase activity Source: WormBase

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BRENDAi3.2.1.52. 1045.
    SABIO-RKQ22492.

    Protein family/group databases

    CAZyiGH20. Glycoside Hydrolase Family 20.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-hexosaminidase A (EC:3.2.1.52)
    Alternative name(s):
    Beta-N-acetylhexosaminidase
    N-acetyl-beta-glucosaminidase
    Gene namesi
    Name:hex-1
    ORF Names:T14F9.3
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome X

    Organism-specific databases

    WormBaseiT14F9.3; CE07499; WBGene00020509; hex-1.

    Subcellular locationi

    Lysosome Curated

    GO - Cellular componenti

    1. lysosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Lysosome

    Pathology & Biotechi

    Disruption phenotypei

    Worms show reduced degradation of p-nitrophenyl-beta-N-acetylglucosaminide and no chitotriosidase activity.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 555537Beta-hexosaminidase APRO_0000012009Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi47 – 471N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi351 – 3511N-linked (GlcNAc...)3 Publications
    Glycosylationi412 – 4121N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi460 – 4601N-linked (GlcNAc...)2 Publications

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ22492.

    Expressioni

    Tissue specificityi

    Expressed in coelomocytes and neurons of the pharyngeal region and nerve cord.1 Publication

    Developmental stagei

    Expressed throughout the life cycle.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ22492.
    SMRiQ22492. Positions 90-547.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 20 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3525.
    GeneTreeiENSGT00390000008107.
    HOGENOMiHOG000157972.
    InParanoidiQ22492.
    KOiK12373.
    OMAiDSESFPY.
    PhylomeDBiQ22492.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.30.379.10. 1 hit.
    InterProiIPR025705. Beta_hexosaminidase_sua/sub.
    IPR029018. Chitobiase/Hex_dom_2-like.
    IPR015883. Glyco_hydro_20_cat-core.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR029019. HEX_eukaryotic_N.
    [Graphical view]
    PfamiPF00728. Glyco_hydro_20. 1 hit.
    PF14845. Glycohydro_20b2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
    PRINTSiPR00738. GLHYDRLASE20.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF55545. SSF55545. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q22492-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRLLIPILIF ALITTAVTWF YGRDDPDRWS VGGVWPLPKK IVYGSKNRTI    50
    TYDKIGIDLG DKKDCDILLS MADNYMNKWL FPFPVEMKTG GTEDFIITVT 100
    VKDECPSGPP VHGASEEYLL RVSLTEAVIN AQTVWGALRA MESLSHLVFY 150
    DHKSQEYQIR TVEIFDKPRF PVRGIMIDSS RHFLSVNVIK RQLEIMSMNK 200
    LNVLHWHLVD SESFPYTSVK FPELHGVGAY SPRHVYSRED IADVIAFARL 250
    RGIRVIPEFD LPGHTSSWRG RKGFLTECFD EKGVETFLPN LVDPMNEANF 300
    DFISEFLEEV TETFPDQFLH LGGDEVSDYI VECWERNKKI RKFMEEKGFG 350
    NDTVLLENYF FEKLYKIVEN LKLKRKPIFW QEVFDNNIPD PNAVIHIWKG 400
    NTHEEIYEQV KNITSQNFPV IVSACWYLNY IKYGADWRDE IRGTAPSNSR 450
    YYYCDPTNFN GTVAQKELVW GGIAAIWGEL VDNTNIEARL WPRASAAAER 500
    LWSPAEKTQR AEDAWPRMHE LRCRLVSRGY RIQPNNNPDY CPFEFDEPPA 550
    TKTEL 555
    Length:555
    Mass (Da):64,379
    Last modified:November 1, 1996 - v1
    Checksum:i5E797F9FD82F6BB4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM748820 mRNA. Translation: CAO72174.1.
    FO081076 Genomic DNA. Translation: CCD68941.1.
    PIRiT29377.
    RefSeqiNP_508409.1. NM_076008.6.
    UniGeneiCel.353.

    Genome annotation databases

    EnsemblMetazoaiT14F9.3; T14F9.3; WBGene00020509.
    GeneIDi180533.
    KEGGicel:CELE_T14F9.3.
    UCSCiT14F9.3. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM748820 mRNA. Translation: CAO72174.1 .
    FO081076 Genomic DNA. Translation: CCD68941.1 .
    PIRi T29377.
    RefSeqi NP_508409.1. NM_076008.6.
    UniGenei Cel.353.

    3D structure databases

    ProteinModelPortali Q22492.
    SMRi Q22492. Positions 90-547.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH20. Glycoside Hydrolase Family 20.

    Proteomic databases

    PaxDbi Q22492.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai T14F9.3 ; T14F9.3 ; WBGene00020509 .
    GeneIDi 180533.
    KEGGi cel:CELE_T14F9.3.
    UCSCi T14F9.3. c. elegans.

    Organism-specific databases

    CTDi 180533.
    WormBasei T14F9.3 ; CE07499 ; WBGene00020509 ; hex-1.

    Phylogenomic databases

    eggNOGi COG3525.
    GeneTreei ENSGT00390000008107.
    HOGENOMi HOG000157972.
    InParanoidi Q22492.
    KOi K12373.
    OMAi DSESFPY.
    PhylomeDBi Q22492.

    Enzyme and pathway databases

    BRENDAi 3.2.1.52. 1045.
    SABIO-RK Q22492.

    Miscellaneous databases

    NextBioi 909772.
    PROi Q22492.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    3.30.379.10. 1 hit.
    InterProi IPR025705. Beta_hexosaminidase_sua/sub.
    IPR029018. Chitobiase/Hex_dom_2-like.
    IPR015883. Glyco_hydro_20_cat-core.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR029019. HEX_eukaryotic_N.
    [Graphical view ]
    Pfami PF00728. Glyco_hydro_20. 1 hit.
    PF14845. Glycohydro_20b2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
    PRINTSi PR00738. GLHYDRLASE20.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF55545. SSF55545. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Biosynthesis of truncated N-linked oligosaccharides results from non-orthologous hexosaminidase-mediated mechanisms in nematodes, plants, and insects."
      Gutternigg M., Kretschmer-Lubich D., Paschinger K., Rendic D., Hader J., Geier P., Ranftl R., Jantsch V., Lochnit G., Wilson I.B.H.
      J. Biol. Chem. 282:27825-27840(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    3. "Lectin affinity capture, isotope-coded tagging and mass spectrometry to identify N-linked glycoproteins."
      Kaji H., Saito H., Yamauchi Y., Shinkawa T., Taoka M., Hirabayashi J., Kasai K., Takahashi N., Isobe T.
      Nat. Biotechnol. 21:667-672(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-351 AND ASN-460, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Bristol N2.
    4. "Identification of the hydrophobic glycoproteins of Caenorhabditis elegans."
      Fan X., She Y.-M., Bagshaw R.D., Callahan J.W., Schachter H., Mahuran D.J.
      Glycobiology 15:952-964(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-351, IDENTIFICATION BY MASS SPECTROMETRY.
    5. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
      Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
      Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-351 AND ASN-460, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Bristol N2.

    Entry informationi

    Entry nameiHEXA_CAEEL
    AccessioniPrimary (citable) accession number: Q22492
    Secondary accession number(s): A7DY65
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3