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Protein

Beta-hexosaminidase A

Gene

hex-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines. Degrades chitotriose.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.1 Publication

Kineticsi

  1. KM=1.1 mM for p-nitrophenyl-beta-N-acetylglucosaminide1 Publication

    pH dependencei

    Optimum pH is 5.5.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei325 – 3251Proton donorBy similarity

    GO - Molecular functioni

    • beta-N-acetylhexosaminidase activity Source: UniProtKB
    • hexosaminidase activity Source: WormBase

    GO - Biological processi

    • carbohydrate metabolic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BRENDAi3.2.1.52. 1045.
    ReactomeiREACT_277085. Hyaluronan uptake and degradation.
    REACT_305285. Keratan sulfate degradation.
    REACT_309213. CS/DS degradation.
    REACT_310934. Glycosphingolipid metabolism.
    SABIO-RKQ22492.

    Protein family/group databases

    CAZyiGH20. Glycoside Hydrolase Family 20.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-hexosaminidase A (EC:3.2.1.52)
    Alternative name(s):
    Beta-N-acetylhexosaminidase
    N-acetyl-beta-glucosaminidase
    Gene namesi
    Name:hex-1
    ORF Names:T14F9.3
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940 Componenti: Chromosome X

    Organism-specific databases

    WormBaseiT14F9.3; CE07499; WBGene00020509; hex-1.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Lysosome

    Pathology & Biotechi

    Disruption phenotypei

    Worms show reduced degradation of p-nitrophenyl-beta-N-acetylglucosaminide and no chitotriosidase activity.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 555537Beta-hexosaminidase APRO_0000012009Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi47 – 471N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi351 – 3511N-linked (GlcNAc...)3 Publications
    Glycosylationi412 – 4121N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi460 – 4601N-linked (GlcNAc...)2 Publications

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ22492.

    Expressioni

    Tissue specificityi

    Expressed in coelomocytes and neurons of the pharyngeal region and nerve cord.1 Publication

    Developmental stagei

    Expressed throughout the life cycle.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi6239.T14F9.3.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ22492.
    SMRiQ22492. Positions 90-547.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 20 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3525.
    GeneTreeiENSGT00390000008107.
    HOGENOMiHOG000157972.
    InParanoidiQ22492.
    KOiK12373.
    OMAiDSESFPY.
    PhylomeDBiQ22492.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.30.379.10. 1 hit.
    InterProiIPR025705. Beta_hexosaminidase_sua/sub.
    IPR029018. Chitobiase/Hex_dom_2-like.
    IPR015883. Glyco_hydro_20_cat-core.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR029019. HEX_eukaryotic_N.
    [Graphical view]
    PfamiPF00728. Glyco_hydro_20. 1 hit.
    PF14845. Glycohydro_20b2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
    PRINTSiPR00738. GLHYDRLASE20.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF55545. SSF55545. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q22492-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRLLIPILIF ALITTAVTWF YGRDDPDRWS VGGVWPLPKK IVYGSKNRTI
    60 70 80 90 100
    TYDKIGIDLG DKKDCDILLS MADNYMNKWL FPFPVEMKTG GTEDFIITVT
    110 120 130 140 150
    VKDECPSGPP VHGASEEYLL RVSLTEAVIN AQTVWGALRA MESLSHLVFY
    160 170 180 190 200
    DHKSQEYQIR TVEIFDKPRF PVRGIMIDSS RHFLSVNVIK RQLEIMSMNK
    210 220 230 240 250
    LNVLHWHLVD SESFPYTSVK FPELHGVGAY SPRHVYSRED IADVIAFARL
    260 270 280 290 300
    RGIRVIPEFD LPGHTSSWRG RKGFLTECFD EKGVETFLPN LVDPMNEANF
    310 320 330 340 350
    DFISEFLEEV TETFPDQFLH LGGDEVSDYI VECWERNKKI RKFMEEKGFG
    360 370 380 390 400
    NDTVLLENYF FEKLYKIVEN LKLKRKPIFW QEVFDNNIPD PNAVIHIWKG
    410 420 430 440 450
    NTHEEIYEQV KNITSQNFPV IVSACWYLNY IKYGADWRDE IRGTAPSNSR
    460 470 480 490 500
    YYYCDPTNFN GTVAQKELVW GGIAAIWGEL VDNTNIEARL WPRASAAAER
    510 520 530 540 550
    LWSPAEKTQR AEDAWPRMHE LRCRLVSRGY RIQPNNNPDY CPFEFDEPPA

    TKTEL
    Length:555
    Mass (Da):64,379
    Last modified:November 1, 1996 - v1
    Checksum:i5E797F9FD82F6BB4
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AM748820 mRNA. Translation: CAO72174.1.
    FO081076 Genomic DNA. Translation: CCD68941.1.
    PIRiT29377.
    RefSeqiNP_508409.1. NM_076008.6.
    UniGeneiCel.353.

    Genome annotation databases

    EnsemblMetazoaiT14F9.3; T14F9.3; WBGene00020509.
    GeneIDi180533.
    KEGGicel:CELE_T14F9.3.
    UCSCiT14F9.3. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AM748820 mRNA. Translation: CAO72174.1.
    FO081076 Genomic DNA. Translation: CCD68941.1.
    PIRiT29377.
    RefSeqiNP_508409.1. NM_076008.6.
    UniGeneiCel.353.

    3D structure databases

    ProteinModelPortaliQ22492.
    SMRiQ22492. Positions 90-547.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi6239.T14F9.3.1.

    Protein family/group databases

    CAZyiGH20. Glycoside Hydrolase Family 20.

    Proteomic databases

    PaxDbiQ22492.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblMetazoaiT14F9.3; T14F9.3; WBGene00020509.
    GeneIDi180533.
    KEGGicel:CELE_T14F9.3.
    UCSCiT14F9.3. c. elegans.

    Organism-specific databases

    CTDi180533.
    WormBaseiT14F9.3; CE07499; WBGene00020509; hex-1.

    Phylogenomic databases

    eggNOGiCOG3525.
    GeneTreeiENSGT00390000008107.
    HOGENOMiHOG000157972.
    InParanoidiQ22492.
    KOiK12373.
    OMAiDSESFPY.
    PhylomeDBiQ22492.

    Enzyme and pathway databases

    BRENDAi3.2.1.52. 1045.
    ReactomeiREACT_277085. Hyaluronan uptake and degradation.
    REACT_305285. Keratan sulfate degradation.
    REACT_309213. CS/DS degradation.
    REACT_310934. Glycosphingolipid metabolism.
    SABIO-RKQ22492.

    Miscellaneous databases

    NextBioi909772.
    PROiQ22492.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.30.379.10. 1 hit.
    InterProiIPR025705. Beta_hexosaminidase_sua/sub.
    IPR029018. Chitobiase/Hex_dom_2-like.
    IPR015883. Glyco_hydro_20_cat-core.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR029019. HEX_eukaryotic_N.
    [Graphical view]
    PfamiPF00728. Glyco_hydro_20. 1 hit.
    PF14845. Glycohydro_20b2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
    PRINTSiPR00738. GLHYDRLASE20.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF55545. SSF55545. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Biosynthesis of truncated N-linked oligosaccharides results from non-orthologous hexosaminidase-mediated mechanisms in nematodes, plants, and insects."
      Gutternigg M., Kretschmer-Lubich D., Paschinger K., Rendic D., Hader J., Geier P., Ranftl R., Jantsch V., Lochnit G., Wilson I.B.H.
      J. Biol. Chem. 282:27825-27840(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    3. "Lectin affinity capture, isotope-coded tagging and mass spectrometry to identify N-linked glycoproteins."
      Kaji H., Saito H., Yamauchi Y., Shinkawa T., Taoka M., Hirabayashi J., Kasai K., Takahashi N., Isobe T.
      Nat. Biotechnol. 21:667-672(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-351 AND ASN-460, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Bristol N2.
    4. "Identification of the hydrophobic glycoproteins of Caenorhabditis elegans."
      Fan X., She Y.-M., Bagshaw R.D., Callahan J.W., Schachter H., Mahuran D.J.
      Glycobiology 15:952-964(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-351, IDENTIFICATION BY MASS SPECTROMETRY.
    5. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
      Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
      Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-351 AND ASN-460, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Bristol N2.

    Entry informationi

    Entry nameiHEXA_CAEEL
    AccessioniPrimary (citable) accession number: Q22492
    Secondary accession number(s): A7DY65
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1996
    Last modified: June 24, 2015
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.