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Protein

Beta-hexosaminidase A

Gene

hex-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines. Degrades chitotriose.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.1 Publication

Kineticsi

  1. KM=1.1 mM for p-nitrophenyl-beta-N-acetylglucosaminide1 Publication

    pH dependencei

    Optimum pH is 5.5.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei325Proton donorBy similarity1

    GO - Molecular functioni

    • beta-N-acetylhexosaminidase activity Source: UniProtKB
    • hexosaminidase activity Source: WormBase
    • N-acetyl-beta-D-galactosaminidase activity Source: UniProtKB-EC

    GO - Biological processi

    • carbohydrate metabolic process Source: UniProtKB

    Keywordsi

    Molecular functionGlycosidase, Hydrolase

    Enzyme and pathway databases

    BRENDAi3.2.1.52. 1045.
    ReactomeiR-CEL-1660662. Glycosphingolipid metabolism.
    R-CEL-2022857. Keratan sulfate degradation.
    R-CEL-2024101. CS/DS degradation.
    R-CEL-2160916. Hyaluronan uptake and degradation.
    R-CEL-6798695. Neutrophil degranulation.
    SABIO-RKiQ22492.

    Protein family/group databases

    CAZyiGH20. Glycoside Hydrolase Family 20.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-hexosaminidase A (EC:3.2.1.52)
    Alternative name(s):
    Beta-N-acetylhexosaminidase
    N-acetyl-beta-glucosaminidase
    Gene namesi
    Name:hex-1
    ORF Names:T14F9.3
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    Proteomesi
    • UP000001940 Componenti: Chromosome X

    Organism-specific databases

    WormBaseiT14F9.3; CE07499; WBGene00020509; hex-1.

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Lysosome

    Pathology & Biotechi

    Disruption phenotypei

    Worms show reduced degradation of p-nitrophenyl-beta-N-acetylglucosaminide and no chitotriosidase activity.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 18Sequence analysisAdd BLAST18
    ChainiPRO_000001200919 – 555Beta-hexosaminidase AAdd BLAST537

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi47N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi351N-linked (GlcNAc...) asparagine3 Publications1
    Glycosylationi412N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi460N-linked (GlcNAc...) asparagine2 Publications1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    EPDiQ22492.
    PaxDbiQ22492.
    PeptideAtlasiQ22492.
    PRIDEiQ22492.

    PTM databases

    iPTMnetiQ22492.

    Expressioni

    Tissue specificityi

    Expressed in coelomocytes and neurons of the pharyngeal region and nerve cord.1 Publication

    Developmental stagei

    Expressed throughout the life cycle.1 Publication

    Gene expression databases

    BgeeiWBGene00020509.

    Interactioni

    Protein-protein interaction databases

    STRINGi6239.T14F9.3.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ22492.
    SMRiQ22492.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 20 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiKOG2499. Eukaryota.
    COG3525. LUCA.
    GeneTreeiENSGT00390000008107.
    HOGENOMiHOG000157972.
    InParanoidiQ22492.
    KOiK12373.
    OMAiHLANMAQ.
    OrthoDBiEOG091G04NA.
    PhylomeDBiQ22492.

    Family and domain databases

    Gene3Di3.30.379.10. 1 hit.
    InterProiView protein in InterPro
    IPR025705. Beta_hexosaminidase_sua/sub.
    IPR029018. Chitobiase/Hex-like_dom2.
    IPR015883. Glyco_hydro_20_cat.
    IPR017853. Glycoside_hydrolase_SF.
    IPR029019. HEX_eukaryotic_N.
    PfamiView protein in Pfam
    PF00728. Glyco_hydro_20. 1 hit.
    PF14845. Glycohydro_20b2. 1 hit.
    PIRSFiPIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
    PRINTSiPR00738. GLHYDRLASE20.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF55545. SSF55545. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q22492-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRLLIPILIF ALITTAVTWF YGRDDPDRWS VGGVWPLPKK IVYGSKNRTI
    60 70 80 90 100
    TYDKIGIDLG DKKDCDILLS MADNYMNKWL FPFPVEMKTG GTEDFIITVT
    110 120 130 140 150
    VKDECPSGPP VHGASEEYLL RVSLTEAVIN AQTVWGALRA MESLSHLVFY
    160 170 180 190 200
    DHKSQEYQIR TVEIFDKPRF PVRGIMIDSS RHFLSVNVIK RQLEIMSMNK
    210 220 230 240 250
    LNVLHWHLVD SESFPYTSVK FPELHGVGAY SPRHVYSRED IADVIAFARL
    260 270 280 290 300
    RGIRVIPEFD LPGHTSSWRG RKGFLTECFD EKGVETFLPN LVDPMNEANF
    310 320 330 340 350
    DFISEFLEEV TETFPDQFLH LGGDEVSDYI VECWERNKKI RKFMEEKGFG
    360 370 380 390 400
    NDTVLLENYF FEKLYKIVEN LKLKRKPIFW QEVFDNNIPD PNAVIHIWKG
    410 420 430 440 450
    NTHEEIYEQV KNITSQNFPV IVSACWYLNY IKYGADWRDE IRGTAPSNSR
    460 470 480 490 500
    YYYCDPTNFN GTVAQKELVW GGIAAIWGEL VDNTNIEARL WPRASAAAER
    510 520 530 540 550
    LWSPAEKTQR AEDAWPRMHE LRCRLVSRGY RIQPNNNPDY CPFEFDEPPA

    TKTEL
    Length:555
    Mass (Da):64,379
    Last modified:November 1, 1996 - v1
    Checksum:i5E797F9FD82F6BB4
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AM748820 mRNA. Translation: CAO72174.1.
    FO081076 Genomic DNA. Translation: CCD68941.1.
    PIRiT29377.
    RefSeqiNP_508409.1. NM_076008.6.
    UniGeneiCel.353.

    Genome annotation databases

    EnsemblMetazoaiT14F9.3; T14F9.3; WBGene00020509.
    GeneIDi180533.
    KEGGicel:CELE_T14F9.3.
    UCSCiT14F9.3. c. elegans.

    Similar proteinsi

    Entry informationi

    Entry nameiHEXA_CAEEL
    AccessioniPrimary (citable) accession number: Q22492
    Secondary accession number(s): A7DY65
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1996
    Last modified: November 22, 2017
    This is version 121 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families