Q22398 (NAS22_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc metalloproteinase nas-22
EC=3.4.24.21

Alternative name(s):
Nematode astacin 22

Gene names

Name:	nas-22
ORF Names:	T11F9.6

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probable metalloprotease By similarity.
Catalytic activity	Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the peptidase M12A family. Contains 1 EGF-like domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	EGF-like domain Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 367	351	Zinc metalloproteinase nas-22		PRO_0000028926
Regions
	Domain	232 – 270	39	EGF-like
Sites
	Active site	139	1	By similarity
	Metal binding	138	1	Zinc; catalytic By similarity
	Metal binding	142	1	Zinc; catalytic By similarity
	Metal binding	148	1	Zinc; catalytic By similarity
Amino acid modifications
	Glycosylation	56	1	N-linked (GlcNAc...) Potential
	Glycosylation	85	1	N-linked (GlcNAc...) Potential
	Glycosylation	169	1	N-linked (GlcNAc...) Potential
	Glycosylation	241	1	N-linked (GlcNAc...) Potential
	Glycosylation	254	1	N-linked (GlcNAc...) Potential
	Glycosylation	287	1	N-linked (GlcNAc...) Potential
	Glycosylation	322	1	N-linked (GlcNAc...) Potential
	Disulfide bond	236 ↔ 244		By similarity
	Disulfide bond	238 ↔ 258		By similarity
	Disulfide bond	260 ↔ 269		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q22398 [UniParc]. Last modified March 23, 2010. Version 3. Checksum: 56D8AAE1B5F497F9	FASTA	367	42,514
	10 20 30 40 50 60 MKSFFILLSI LQECYGKDIV ARIGGRNVAE KIGGARHRRQ VLIRGSDEER RHKWFNNTVH 70 80 90 100 110 120 YYFYEENFDF TVKESILRAM ELISNHTCIK FSTEPSEKSI RMESDSTTIA CYAEIGQVRE 130 140 150 160 170 180 NQLFSFNSDC YSAGVAVHEL IHSLGFIHAH QRSDRDQYLE FKKNLDELNQ TYQEQYKIWE 190 200 210 220 230 240 YQEILVPYDV GSVMQYPNEE DEEYYPVRKY RTMANTMGSA IVAFYDYLMI NKYYECSCAN 250 260 270 280 290 300 NLSCKNHGYP NPSNCSQCNC PYGFGGADCS QRAEPGATFQ ATETWQNVTI SLDAGYRYLE 310 320 330 340 350 360 NNQKLPQVDF IYQFLWIMAP ANKTTQIRVE KFVEGKCLPG CIRGGVEIKT NEDPRLTSPR LCCEETS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"The astacin protein family in Caenorhabditis elegans." Moehrlen F., Hutter H., Zwilling R. Eur. J. Biochem. 270:4909-4920(2003) [PubMed: 14653817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 133-195, NOMENCLATURE. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z74042 Genomic DNA. Translation: CAA98530.2. AJ561212 mRNA. Translation: CAE47484.1.
PIR	T24838.
UniGene	Cel.2325.
3D structure databases
ProteinModelPortal	Q22398.
SMR	Q22398. Positions 48-236.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-26108N.
MINT	MINT-1092334.
Protein family/group databases
MEROPS	M12.A43.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	T11F9.6; T11F9.6; T11F9.6.
UCSC	T11F9.6. c. elegans.
Organism-specific databases
WormBase	T11F9.6; CE44156; WBGene00003541; nas-22.
Phylogenomic databases
eggNOG	meNOG13023.
Gene expression databases
ArrayExpress	Q22398.
Family and domain databases
InterPro	IPR013032. EGF-like_reg_CS. IPR024079. MetalloPept_cat_dom. IPR001506. Peptidase_M12A. IPR006026. Peptidase_Metallo. [Graphical view]
Gene3D	G3DSA:3.40.390.10. G3DSA:3.40.390.10. 1 hit.
Pfam	PF01400. Astacin. 1 hit. [Graphical view]
PRINTS	PR00480. ASTACIN.
SMART	SM00235. ZnMc. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAS22_CAEEL

Accession

Primary (citable) accession number: Q22398
Secondary accession number(s): Q70MS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	March 23, 2010
Last modified:	November 16, 2011
This is version 85 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project