Reviewed,
UniProtKB/Swiss-Prot Q22398 (NAS22_CAEEL)
Last modified
June 16, 2009.
Version 66.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Zinc metalloproteinase nas-22 EC=3.4.24.21 Alternative name(s): Nematode astacin 22 | ||||
| Gene names |
| ||||
| Organism | Caenorhabditis elegans [Complete proteome] | ||||
| Taxonomic identifier | 6239 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Protein attributes
| Sequence length | 595 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Probable metalloprotease By similarity. |
| Catalytic activity | Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'. |
| Cofactor | Binds 1 zinc ion per subunit By similarity. |
| Subcellular location | Secreted Potential. |
| Sequence similarities | Belongs to the peptidase M12A family. Contains 1 EGF-like domain. |
| Sequence caution | The sequence CAA98530.1 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Secreted |
| Domain | EGF-like domain Signal |
| Ligand | Metal-binding Zinc |
| Molecular function | Hydrolase Metalloprotease Protease |
| PTM | Disulfide bond Glycoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | proteolysis Inferred from electronic annotation. Source: InterPro |
| Cellular component | extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 16 | 16 | Potential | ||||||||
| Chain | 17 – 595 | 579 | Zinc metalloproteinase nas-22 | PRO_0000028926 | |||||||
Regions | |||||||||||
| Domain | 460 – 498 | 39 | EGF-like | ||||||||
| Compositional bias | 352 – 357 | 6 | Poly-Arg | ||||||||
Sites | |||||||||||
| Active site | 139 | 1 | By similarity | ||||||||
| Metal binding | 138 | 1 | Zinc; catalytic By similarity | ||||||||
| Metal binding | 142 | 1 | Zinc; catalytic By similarity | ||||||||
| Metal binding | 148 | 1 | Zinc; catalytic By similarity | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 56 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 85 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 169 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 265 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 469 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 482 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 515 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 550 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 464 ↔ 472 | By similarity | |||||||||
| Disulfide bond | 466 ↔ 486 | By similarity | |||||||||
| Disulfide bond | 488 ↔ 497 | By similarity | |||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2. |
| [2] | "The astacin protein family in Caenorhabditis elegans." Moehrlen F., Hutter H., Zwilling R. Eur. J. Biochem. 270:4909-4920(2003) [PubMed: 14653817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 133-195, NOMENCLATURE. Strain: Bristol N2. |
Cross-references
Sequence databases | |
|---|---|
| Z74042 Genomic DNA. Translation: CAA98530.1. Sequence problems. AJ561212 mRNA. Translation: CAE47484.1. | |
| PIR | T24838. |
| UniGene | Cel.2325 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:26108N. |
| IntAct | Q22398. 1 interaction. |
Protein family/group databases | |
| MEROPS | M12.310. |
Genome annotation databases | |
| Ensembl | T11F9.6. Caenorhabditis elegans. [Contig view] |
Organism-specific databases | |
| WormBase | WBGene00003541. nas-22. |
| WormPep | T11F9.6. CE06418. [WorfDB] |
Enzyme and pathway databases | |
| BRENDA | 3.4.24.21. 672. |
Gene expression databases | |
| ArrayExpress | Q22398. |
Family and domain databases | |
| InterPro | IPR013032. EGF-like_reg_CS. IPR006025. Pept_M_Zn_BS. IPR006026. Peptidase_M. IPR001506. Peptidase_M12A. [Graphical view] |
| Pfam | PF01400. Astacin. 2 hits. [Graphical view] |
| PRINTS | PR00480. ASTACIN. |
| SMART | SM00235. ZnMc. 1 hit. [Graphical view] |
| PROSITE | PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | NAS22_CAEEL | ||||||||
| Accession | Primary (citable) accession number: Q22398 Secondary accession number(s): Q70MS9 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | Caenorhabditis annotation project | ||||||||
Relevant documents
| Caenorhabditis elegans Caenorhabditis elegans: entries, gene names and cross-references to WormPep |
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


