Reviewed, UniProtKB/Swiss-Prot Q22398 (NAS22_CAEEL)

Names and origin

Protein names

Recommended name:
    Zinc metalloproteinase nas-22
    EC=3.4.24.21
Alternative name(s):
    Nematode astacin 22

Gene names

Name:	nas-22
ORF Names:	T11F9.6

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	595 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable metalloprotease By similarity.
Catalytic activity	Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the peptidase M12A family. Contains 1 EGF-like domain.
Sequence caution	The sequence CAA98530.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Secreted
Domain	EGF-like domain Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 595	579	Zinc metalloproteinase nas-22		PRO_0000028926
Regions
	Domain	460 – 498	39	EGF-like
	Compositional bias	352 – 357	6	Poly-Arg
Sites
	Active site	139	1	By similarity
	Metal binding	138	1	Zinc; catalytic By similarity
	Metal binding	142	1	Zinc; catalytic By similarity
	Metal binding	148	1	Zinc; catalytic By similarity
Amino acid modifications
	Glycosylation	56	1	N-linked (GlcNAc...) Potential
	Glycosylation	85	1	N-linked (GlcNAc...) Potential
	Glycosylation	169	1	N-linked (GlcNAc...) Potential
	Glycosylation	265	1	N-linked (GlcNAc...) Potential
	Glycosylation	469	1	N-linked (GlcNAc...) Potential
	Glycosylation	482	1	N-linked (GlcNAc...) Potential
	Glycosylation	515	1	N-linked (GlcNAc...) Potential
	Glycosylation	550	1	N-linked (GlcNAc...) Potential
	Disulfide bond	464 ↔ 472		By similarity
	Disulfide bond	466 ↔ 486		By similarity
	Disulfide bond	488 ↔ 497		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q22398-1 [UniParc]. Last modified January 4, 2005. Version 2. Checksum: B750BD854DCB4385	FASTA	595	69,381
	10 20 30 40 50 60 MKSFFILLSI LQECYGKDIV ARIGGRNVAE KIGGARHRRQ VLIRGSDEER RHKWFNNTVH 70 80 90 100 110 120 YYFYEENFDF TVKESILRAM ELISNHTCIK FSTEPSEKSI RMESDSTTIA CYAEIGQVRE 130 140 150 160 170 180 NQLFSFNSDC YSAGVAVHEL IHSLGFIHAH QRSDRDQYLE FKKNLDELNQ TYQEQYKIWE 190 200 210 220 230 240 YQEILVPYDV GSVMQYPNEE MEDESSAKYP YRESMPRAER VSMKALQDEW RHLFITRAEG 250 260 270 280 290 300 ENNIHNPKIP EDTNDFDDDM PIECNESEEE SITGDREVFS VHHNEEDEDQ EDSSQICEES 310 320 330 340 350 360 EPQELFEMFD LSLDISGLYL PQTLLTGRID LNDEFVASIE VEDAEKSKEI LRRRRERPLA 370 380 390 400 410 420 LRQFSHALSR AMANLRTYHY DVTTEKIERC PMRNSCKGHL KWEQMRKYRA QCKKNFKDPS 430 440 450 460 470 480 LATVEDMIDE EYYPVRKYRT MANTMGSAIV AFYDYLMINK YYECSCANNL SCKNHGYPNP 490 500 510 520 530 540 SNCSQCNCPY GFGGADCSQR AEPGATFQAT ETWQNVTISL DAGYRYLENN QKLPQVDFIY 550 560 570 580 590 QFLWIMAPAN KTTQIRVEKF VEGKCLPGCI RGGVEIKTNE DPRLTSPRLC CEETS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"The astacin protein family in Caenorhabditis elegans." Moehrlen F., Hutter H., Zwilling R. Eur. J. Biochem. 270:4909-4920(2003) [PubMed: 14653817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 133-195, NOMENCLATURE. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z74042 Genomic DNA. Translation: CAA98530.1. Sequence problems. AJ561212 mRNA. Translation: CAE47484.1.
PIR	T24838.
UniGene	Cel.2325
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:26108N.
IntAct	Q22398. 1 interaction.
Protein family/group databases
MEROPS	M12.310.
Genome annotation databases
Ensembl	T11F9.6. Caenorhabditis elegans. [Contig view]
Organism-specific databases
WormBase	WBGene00003541. nas-22.
WormPep	T11F9.6. CE06418. [WorfDB]
Enzyme and pathway databases
BRENDA	3.4.24.21. 672.
Gene expression databases
ArrayExpress	Q22398.
Family and domain databases
InterPro	IPR013032. EGF-like_reg_CS. IPR006025. Pept_M_Zn_BS. IPR006026. Peptidase_M. IPR001506. Peptidase_M12A. [Graphical view]
Pfam	PF01400. Astacin. 2 hits. [Graphical view]
PRINTS	PR00480. ASTACIN.
SMART	SM00235. ZnMc. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAS22_CAEEL

Accession

Primary (citable) accession number: Q22398
Secondary accession number(s): Q70MS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	January 4, 2005
Last modified:	June 16, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project