Reviewed, UniProtKB/Swiss-Prot Q22396 (NAS20_CAEEL)

Names and origin

Protein names

Recommended name:
    Zinc metalloproteinase nas-20
    EC=3.4.24.21
Alternative name(s):
    Nematode astacin 20

Gene names

Name:	nas-20
ORF Names:	T11F9.3

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probable metalloprotease By similarity.
Catalytic activity	Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the peptidase M12A family. Contains 1 EGF-like domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	EGF-like domain Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 379	359	Zinc metalloproteinase nas-20		PRO_0000028924
Regions
	Domain	203 – 244	42	EGF-like
Sites
	Active site	120	1	By similarity
	Metal binding	119	1	Zinc; catalytic By similarity
	Metal binding	123	1	Zinc; catalytic By similarity
	Metal binding	129	1	Zinc; catalytic By similarity
Amino acid modifications
	Glycosylation	67	1	N-linked (GlcNAc...) Potential
	Glycosylation	185	1	N-linked (GlcNAc...) Ref.3
	Glycosylation	337	1	N-linked (GlcNAc...) Ref.3
	Glycosylation	370	1	N-linked (GlcNAc...) Potential
	Disulfide bond	207 ↔ 218		By similarity
	Disulfide bond	209 ↔ 229		By similarity
	Disulfide bond	234 ↔ 243		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q22396-1 [UniParc]. Last modified July 5, 2004. Version 2. Checksum: E79F82C99226FB51	FASTA	379	43,378
	10 20 30 40 50 60 MKITVNFLLV ALIGVPSVLS DRHITRDKRQ AMRDYAKWEN NKMSLFFYNL PLEMQAMFRD 70 80 90 100 110 120 AINYLENHTC LKFEYNENAE TAVRIRKGNG CYSLYGMHAG EVQDLTLDYN CASFGTAVHE 130 140 150 160 170 180 IMHALGIAHG QARSDRDDYL IVDSTNSNDG IENTENLVPF DYGSVMLYAR DPHSDKRIPI 190 200 210 220 230 240 DPEYNFTMGS LRVAFYDMVL LNKFYGCNCD NHPRKLDCKN GGYQNPANCE ECLCTDGFNG 250 260 270 280 290 300 QLCDQHEGVY VLEAKKEWDA SGVRNNYRKG IETNTMPEYT YFALTAPEGS TIEVRITKLS 310 320 330 340 350 360 GFFCQHTCDY NGVELKYKTD RRIVSPLVCC DNDNLWNKTR SSTNNPFIIA KYGNNRTPHF 370 EFEYRYIPGN ATAAPEENN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"The astacin protein family in Caenorhabditis elegans." Moehrlen F., Hutter H., Zwilling R. Eur. J. Biochem. 270:4909-4920(2003) [PubMed: 14653817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 158-308, NOMENCLATURE. Strain: Bristol N2.
[3]	"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins." Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T. Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed: 17761667] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-185 AND ASN-337, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z74042 Genomic DNA. Translation: CAA98528.2. AJ561210 mRNA. Translation: CAD99212.1.
PIR	T24836.
RefSeq	NP_505906.2.
UniGene	Cel.34453
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M12.310.
Genome annotation databases
Ensembl	T11F9.3. Caenorhabditis elegans. [Contig view]
GeneID	188420.
KEGG	cel:T11F9.3.
NMPDR	fig|6239.3.peg.19736.
Organism-specific databases
WormBase	WBGene00003539. nas-20.
WormPep	T11F9.3. CE35907. [WorfDB]
Phylogenomic databases
OMA	Q22396. FEFEYRY.
Enzyme and pathway databases
BRENDA	3.4.24.21. 672.
Gene expression databases
ArrayExpress	Q22396.
Family and domain databases
InterPro	IPR013032. EGF-like_reg_CS. IPR006025. Pept_M_Zn_BS. IPR006026. Peptidase_M. IPR001506. Peptidase_M12A. IPR017050. Peptidase_M12A_nem. [Graphical view]
Pfam	PF01400. Astacin. 1 hit. [Graphical view]
PIRSF	PIRSF036365. Astacin_nematoda. 1 hit.
PRINTS	PR00480. ASTACIN.
SMART	SM00235. ZnMc. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	938732.

Entry information

Entry name

NAS20_CAEEL

Accession

Primary (citable) accession number: Q22396
Secondary accession number(s): Q7Z0M9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project