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Protein

Zinc metalloproteinase nas-20

Gene

nas-20

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probable metalloprotease.By similarity

Catalytic activityi

Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi119 – 1191Zinc; catalyticPROSITE-ProRule annotation
Active sitei120 – 1201PROSITE-ProRule annotation
Metal bindingi123 – 1231Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi129 – 1291Zinc; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.A42.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase nas-20 (EC:3.4.24.21)
Alternative name(s):
Nematode astacin 20
Gene namesi
Name:nas-20
ORF Names:T11F9.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiT11F9.3; CE35907; WBGene00003539; nas-20.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 379359Zinc metalloproteinase nas-20PRO_0000028924Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi67 – 671N-linked (GlcNAc...)Sequence analysis
Glycosylationi185 – 1851N-linked (GlcNAc...)1 Publication
Disulfide bondi207 ↔ 218By similarity
Disulfide bondi209 ↔ 229By similarity
Disulfide bondi234 ↔ 243By similarity
Glycosylationi337 – 3371N-linked (GlcNAc...)1 Publication
Glycosylationi370 – 3701N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ22396.
PaxDbiQ22396.
PRIDEiQ22396.

Expressioni

Gene expression databases

BgeeiWBGene00003539.

Interactioni

Protein-protein interaction databases

STRINGi6239.T11F9.3.

Structurei

3D structure databases

ProteinModelPortaliQ22396.
SMRiQ22396. Positions 56-207.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini203 – 24442EGF-likeAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M12A family.Curated
Contains 1 EGF-like domain.Curated

Keywords - Domaini

EGF-like domain, Signal

Phylogenomic databases

eggNOGiKOG3714. Eukaryota.
ENOG410ZPX7. LUCA.
GeneTreeiENSGT00760000119227.
HOGENOMiHOG000022523.
InParanoidiQ22396.
KOiK08076.
OMAiLEMQAMF.
OrthoDBiEOG091G0EQ3.
PhylomeDBiQ22396.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR017050. Metallopeptidase_nem.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
[Graphical view]
PIRSFiPIRSF036365. Astacin_nematoda. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00235. ZnMc. 1 hit.
[Graphical view]
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q22396-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKITVNFLLV ALIGVPSVLS DRHITRDKRQ AMRDYAKWEN NKMSLFFYNL
60 70 80 90 100
PLEMQAMFRD AINYLENHTC LKFEYNENAE TAVRIRKGNG CYSLYGMHAG
110 120 130 140 150
EVQDLTLDYN CASFGTAVHE IMHALGIAHG QARSDRDDYL IVDSTNSNDG
160 170 180 190 200
IENTENLVPF DYGSVMLYAR DPHSDKRIPI DPEYNFTMGS LRVAFYDMVL
210 220 230 240 250
LNKFYGCNCD NHPRKLDCKN GGYQNPANCE ECLCTDGFNG QLCDQHEGVY
260 270 280 290 300
VLEAKKEWDA SGVRNNYRKG IETNTMPEYT YFALTAPEGS TIEVRITKLS
310 320 330 340 350
GFFCQHTCDY NGVELKYKTD RRIVSPLVCC DNDNLWNKTR SSTNNPFIIA
360 370
KYGNNRTPHF EFEYRYIPGN ATAAPEENN
Length:379
Mass (Da):43,378
Last modified:July 5, 2004 - v2
Checksum:iE79F82C99226FB51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74042 Genomic DNA. Translation: CAA98528.2.
AJ561210 mRNA. Translation: CAD99212.1.
PIRiT24836.
RefSeqiNP_505906.2. NM_073505.6.
UniGeneiCel.34453.

Genome annotation databases

EnsemblMetazoaiT11F9.3; T11F9.3; WBGene00003539.
GeneIDi188420.
KEGGicel:CELE_T11F9.3.
UCSCiT11F9.3. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74042 Genomic DNA. Translation: CAA98528.2.
AJ561210 mRNA. Translation: CAD99212.1.
PIRiT24836.
RefSeqiNP_505906.2. NM_073505.6.
UniGeneiCel.34453.

3D structure databases

ProteinModelPortaliQ22396.
SMRiQ22396. Positions 56-207.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.T11F9.3.

Protein family/group databases

MEROPSiM12.A42.

Proteomic databases

EPDiQ22396.
PaxDbiQ22396.
PRIDEiQ22396.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT11F9.3; T11F9.3; WBGene00003539.
GeneIDi188420.
KEGGicel:CELE_T11F9.3.
UCSCiT11F9.3. c. elegans.

Organism-specific databases

CTDi188420.
WormBaseiT11F9.3; CE35907; WBGene00003539; nas-20.

Phylogenomic databases

eggNOGiKOG3714. Eukaryota.
ENOG410ZPX7. LUCA.
GeneTreeiENSGT00760000119227.
HOGENOMiHOG000022523.
InParanoidiQ22396.
KOiK08076.
OMAiLEMQAMF.
OrthoDBiEOG091G0EQ3.
PhylomeDBiQ22396.

Miscellaneous databases

PROiQ22396.

Gene expression databases

BgeeiWBGene00003539.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR017050. Metallopeptidase_nem.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
[Graphical view]
PIRSFiPIRSF036365. Astacin_nematoda. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00235. ZnMc. 1 hit.
[Graphical view]
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAS20_CAEEL
AccessioniPrimary (citable) accession number: Q22396
Secondary accession number(s): Q7Z0M9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.