Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Rab-3-interacting molecule unc-10

Gene

unc-10

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates the efficiency of a post-docking step of the release pathway. Acts after vesicle docking likely via regulating priming. May regulate the conformational changes in syntaxin. Binding of vesicles via rab-3[GTP] to Rim may signal the presence of a docked synaptic vesicle. Rim may then signal to unc-13 to change the conformation of syntaxin from the closed to the open state. Syntaxin could then engage synaptobrevin on the docked vesicle to form SNARE complexes and to prime the vesicle for release. Not required for the development or the structural organization of synapses.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri66 – 121FYVE-typePROSITE-ProRule annotationAdd BLAST56

GO - Molecular functioni

  • ion channel binding Source: GO_Central
  • metal ion binding Source: UniProtKB-KW
  • protein C-terminus binding Source: WormBase
  • Rab GTPase binding Source: WormBase

GO - Biological processi

  • calcium ion-regulated exocytosis of neurotransmitter Source: GO_Central
  • defecation Source: WormBase
  • intracellular protein transport Source: InterPro
  • locomotion Source: WormBase
  • locomotory behavior Source: WormBase
  • mating behavior Source: WormBase
  • positive regulation of multicellular organism growth Source: WormBase
  • positive regulation of synaptic transmission Source: WormBase
  • positive regulation of synaptic transmission, cholinergic Source: WormBase
  • protein localization Source: WormBase
  • regulation of membrane potential Source: GO_Central
  • regulation of pharyngeal pumping Source: WormBase
  • regulation of synaptic vesicle exocytosis Source: GO_Central
  • synaptic transmission, cholinergic Source: WormBase
  • synaptic vesicle priming Source: WormBase
Complete GO annotation...

Keywords - Biological processi

Exocytosis

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Rab-3-interacting molecule unc-10
Short name:
Rim
Alternative name(s):
Uncoordinated protein 10
Gene namesi
Name:unc-10
ORF Names:T10A3.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiT10A3.1a; CE30169; WBGene00006750; unc-10.
T10A3.1b; CE31234; WBGene00006750; unc-10.

Subcellular locationi

GO - Cellular componenti

  • axon Source: WormBase
  • cell junction Source: UniProtKB-KW
  • intracellular Source: GOC
  • presynaptic active zone Source: WormBase
  • synapse Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001902101 – 1563Rab-3-interacting molecule unc-10Add BLAST1563

Proteomic databases

PaxDbiQ22366.
PeptideAtlasiQ22366.
PRIDEiQ22366.

PTM databases

iPTMnetiQ22366.

Expressioni

Tissue specificityi

Restricted to discrete puncta in synapse-rich regions of the nervous system including the nerve ring, the ventral nerve cord and the dorsal nerve cord. Localized expression was found in the head.1 Publication

Developmental stagei

Observed in all larval stages.1 Publication

Gene expression databases

BgeeiWBGene00006750.

Interactioni

Subunit structurei

Interacts with rab-3 and unc-13.1 Publication

GO - Molecular functioni

  • ion channel binding Source: GO_Central
  • protein C-terminus binding Source: WormBase
  • Rab GTPase binding Source: WormBase

Protein-protein interaction databases

IntActiQ22366. 7 interactors.
MINTiMINT-3384948.
STRINGi6239.T10A3.1a.

Structurei

3D structure databases

ProteinModelPortaliQ22366.
SMRiQ22366.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 133RabBDPROSITE-ProRule annotationAdd BLAST127
Domaini643 – 733PDZPROSITE-ProRule annotationAdd BLAST91
Domaini842 – 946C2 1PROSITE-ProRule annotationAdd BLAST105
Domaini1417 – 1519C2 2PROSITE-ProRule annotationAdd BLAST103

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi117 – 420Asn/Gln-richAdd BLAST304
Compositional biasi1176 – 1236Gln-richAdd BLAST61

Sequence similaritiesi

Contains 2 C2 domains.PROSITE-ProRule annotation
Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 RabBD (Rab-binding) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri66 – 121FYVE-typePROSITE-ProRule annotationAdd BLAST56

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2060. Eukaryota.
KOG3528. Eukaryota.
KOG3799. Eukaryota.
ENOG410ZNB1. LUCA.
GeneTreeiENSGT00550000074588.
HOGENOMiHOG000019307.
InParanoidiQ22366.
KOiK15297.
OMAiITETDYD.
OrthoDBiEOG091G0IYY.
PhylomeDBiQ22366.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
2.60.40.150. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR000008. C2_dom.
IPR001478. PDZ.
IPR010911. Rab_BD.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00168. C2. 2 hits.
PF02318. FYVE_2. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 2 hits.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
SSF50156. SSF50156. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEiPS50004. C2. 2 hits.
PS50106. PDZ. 1 hit.
PS50916. RABBD. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q22366-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDDPSMMPDL SHLSAEEREI IENVFKRQKD EEAKETQISQ KASEELSELD
60 70 80 90 100
KQITERKETS KKLVGTQDDA ICQICQKTKF ADGIGHKCFY CQLRSCARCG
110 120 130 140 150
GRAQSKNKAI WACSLCQKRQ QILAKTGKWF QPEEQPQPKI SGSEPSPSPQ
160 170 180 190 200
PLTDDNVPEP QQRRAEPPDK MNTPNYQNNQ QPRGMGMQPN HNQTQQNFMN
210 220 230 240 250
QNQNSNQHPN QNHNQNQMQN PHQNQNHVQN NHQGANNHQQ NNRRAMQQQP
260 270 280 290 300
MSQNQANQIN QMNQNQNQQQ SHNQNMTQNQ RNQTGPQNQQ RTNDSRTMKQ
310 320 330 340 350
TPQQQPSQYQ NNVGAAHQHH NQHGQQEQHH QQMNEQRTDN NRMRENTNGQ
360 370 380 390 400
GGMFNRQPSL EQTTPMNKYN HVEDDGMNQR PTFYTGNSEN DQRQFDGQMQ
410 420 430 440 450
QGSQQNNQNQ NQNNRNLRKN TVSRVTEEDY ASSSNFESKK QRNNSSQSQS
460 470 480 490 500
NTQGVRACPS TDDHLNRVKN RLHRQLRSMS SSEEDIIAGG GGNTLKMSTS
510 520 530 540 550
AVVASGGKTA FHDDMGASNV QRLSEECNSE KDLLRYIYGD HKNSDSSSLG
560 570 580 590 600
AGVGGSGGGG VLSGNSVLKS KSHALLKGSY QGGLDMQANR RRDKSLSLSP
610 620 630 640 650
SRNDHFGTGS ISGGDLLASR IRTFLSHPVT WQPSADQKKL IGHMILHRTE
660 670 680 690 700
NSAANGDLGL KIVGGRRTDT GKLGAFITQV KPGSVADTIG RLRPGDEVVE
710 720 730 740 750
WNGQSLQNAT YEQVYDSIAA SRYDTSVELI VSRSAIIPGG DDFLNLTPSQ
760 770 780 790 800
MSSSAYSRVP SAYPSQFQRQ LPNPDLFLDI HPALQQLSLP HSQSAVFPHN
810 820 830 840 850
NTLTSRNRST SSYYYSDVPD LGVPSNREMQ ESQAFGTGHI FGRIEVSFVY
860 870 880 890 900
SHHDRQLSVA LVRGFDLPPR SDGTPRNPYV KIFLLPDRSE KSRRQSAVIA
910 920 930 940 950
ETLMPVWDEV FYYNGLTEPM LLQRVLELTV WDYDKFGTNS FLGETLIDLA
960 970 980 990 1000
SVPLDGEHSL MCILVDMDDD NPLRTVISIF KFHVIILTFL QRLKLRKASY
1010 1020 1030 1040 1050
NAPTRRPQSE LNYYDHSSNY YDHISQNIDK QPHHHHLAPN DEENDEYIDD
1060 1070 1080 1090 1100
DELENDIDLA TGGGARKSRT YRREKGMHGG HGYADWTQNH QRQSGYTSDH
1110 1120 1130 1140 1150
GYGRQNMIGR AYNRRQQRRP RSATALSQME REDMYDPTRK HRDDNEYSMR
1160 1170 1180 1190 1200
ESVRHGSQYY LGDQPLYEDG RYKISQGQMT PKQHNQQHQP HPLSQAHQQQ
1210 1220 1230 1240 1250
QTAGVQPQHH QGFQQQQHPQ QPNQQMQQMQ PPMPNQGYYS DGSETLSVHS
1260 1270 1280 1290 1300
TNSMPTTMTT VNRRNMNANN TSNDNTSFAE TPTANTNRVP IKETKQNSLA
1310 1320 1330 1340 1350
SSSSVAGGGS AANNVMKERK KSLMTRFIPG RGAEGKRTGF ARSEEVGIPG
1360 1370 1380 1390 1400
NLSSDRLTEP TPPFLKQASK ESTDSAHSDK FARQCWLPVL ADGPLGTFVD
1410 1420 1430 1440 1450
NLGPGQVVGR QVLASPVLGE IQIALMAGRS GIDVEIIKAK NLVVKPGVKV
1460 1470 1480 1490 1500
CPAPYVKVYL MEGKQCIAKA KTNAATKTTS PLFQQHLIFN DSPKKKTLQV
1510 1520 1530 1540 1550
TVLGDYGRME RKVFMGISQI RLEDLELGSQ PLIGWYKLFH SSSLAGTGPV
1560
RKDSDVSVGG AQQ
Length:1,563
Mass (Da):175,479
Last modified:June 1, 2002 - v2
Checksum:i77EE93619C24E899
GO
Isoform b (identifier: Q22366-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     976-991: Missing.
     1386-1387: WL → AR
     1388-1563: Missing.

Note: No experimental confirmation available.
Show »
Length:1,371
Mass (Da):154,681
Checksum:iB6D7D918E58EFB71
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009139976 – 991Missing in isoform b. CuratedAdd BLAST16
Alternative sequenceiVSP_0091401386 – 1387WL → AR in isoform b. Curated2
Alternative sequenceiVSP_0091411388 – 1563Missing in isoform b. CuratedAdd BLAST176

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF257062 mRNA. Translation: AAK49331.1.
FO081400 Genomic DNA. Translation: CCD71364.1.
FO081400 Genomic DNA. Translation: CCD71365.1.
PIRiT29736.
RefSeqiNP_001024901.1. NM_001029730.3. [Q22366-2]
NP_741831.1. NM_171720.3. [Q22366-1]
UniGeneiCel.6180.

Genome annotation databases

EnsemblMetazoaiT10A3.1a; T10A3.1a; WBGene00006750. [Q22366-1]
GeneIDi180990.
KEGGicel:CELE_T10A3.1.
UCSCiT10A3.1b. c. elegans. [Q22366-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF257062 mRNA. Translation: AAK49331.1.
FO081400 Genomic DNA. Translation: CCD71364.1.
FO081400 Genomic DNA. Translation: CCD71365.1.
PIRiT29736.
RefSeqiNP_001024901.1. NM_001029730.3. [Q22366-2]
NP_741831.1. NM_171720.3. [Q22366-1]
UniGeneiCel.6180.

3D structure databases

ProteinModelPortaliQ22366.
SMRiQ22366.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ22366. 7 interactors.
MINTiMINT-3384948.
STRINGi6239.T10A3.1a.

PTM databases

iPTMnetiQ22366.

Proteomic databases

PaxDbiQ22366.
PeptideAtlasiQ22366.
PRIDEiQ22366.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT10A3.1a; T10A3.1a; WBGene00006750. [Q22366-1]
GeneIDi180990.
KEGGicel:CELE_T10A3.1.
UCSCiT10A3.1b. c. elegans. [Q22366-1]

Organism-specific databases

CTDi180990.
WormBaseiT10A3.1a; CE30169; WBGene00006750; unc-10.
T10A3.1b; CE31234; WBGene00006750; unc-10.

Phylogenomic databases

eggNOGiKOG2060. Eukaryota.
KOG3528. Eukaryota.
KOG3799. Eukaryota.
ENOG410ZNB1. LUCA.
GeneTreeiENSGT00550000074588.
HOGENOMiHOG000019307.
InParanoidiQ22366.
KOiK15297.
OMAiITETDYD.
OrthoDBiEOG091G0IYY.
PhylomeDBiQ22366.

Miscellaneous databases

PROiQ22366.

Gene expression databases

BgeeiWBGene00006750.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
2.60.40.150. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR000008. C2_dom.
IPR001478. PDZ.
IPR010911. Rab_BD.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00168. C2. 2 hits.
PF02318. FYVE_2. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 2 hits.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
SSF50156. SSF50156. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEiPS50004. C2. 2 hits.
PS50106. PDZ. 1 hit.
PS50916. RABBD. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIM_CAEEL
AccessioniPrimary (citable) accession number: Q22366
Secondary accession number(s): Q22367, Q8MPX5, Q95WX9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: June 1, 2002
Last modified: November 2, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.