Reviewed, UniProtKB/Swiss-Prot Q22347 (ACADM_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable medium-chain specific acyl-CoA dehydrogenase, mitochondrial
      Short name=MCAD
    EC=1.3.99.3

Gene names

ORF Names:

T08G2.3

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This enzyme is specific for acyl chain lengths of 4 to 16 By similarity.
Catalytic activity	Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor.
Cofactor	FAD By similarity.
Pathway	Lipid metabolism; mitochondrial fatty acid beta-oxidation.
Subunit structure	Homotetramer By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the acyl-CoA dehydrogenase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro acyl-CoA dehydrogenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 15	15	Mitochondrion By similarity
	Chain	16 – 417	402	Probable medium-chain specific acyl-CoA dehydrogenase, mitochondrial		PRO_0000000507
Regions
	Nucleotide binding	148 – 157	10	FAD By similarity
	Nucleotide binding	181 – 183	3	FAD By similarity
	Nucleotide binding	306 – 307	2	FAD By similarity
	Nucleotide binding	364 – 368	5	FAD By similarity
	Nucleotide binding	393 – 395	3	FAD By similarity
	Region	268 – 271	4	Substrate binding By similarity
Sites
	Active site	391	1	Proton acceptor By similarity
	Binding site	157	1	Substrate; via carbonyl oxygen By similarity
	Binding site	392	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q22347-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 98CE260E72C521BF	FASTA	417	44,818
	10 20 30 40 50 60 MLSRIATSSL GLSRSATGVI ATQSRQISFD LSETQKEIQD AALKFSKDVL VPNAAKFDES 70 80 90 100 110 120 GEFPWEIVRQ AHSLGLMNPQ IPEKYGGPGM TTLETALIVE ALSYGCTGIQ LGIMGPSLAI 130 140 150 160 170 180 APVYISGNEE QKKKYLGALA AEPIIASYCV TEPGAGSDVN GVKTKCEKKG DEYIINGSKA 190 200 210 220 230 240 WITGGGHAKW FFVLARSDPN PKTPAGKAFT AFIVDGDTPG ITRGKKEKNM GQRCSDTRVI 250 260 270 280 290 300 TFEDVRVPAE NVLGAPGAGF KVAMEAFDMT RPGVAAGALG LSWRCLDESA KYALERKAFG 310 320 330 340 350 360 TVIANHQAVQ FMLADMAVNL ELARLITYKS ANDVDNKVRS SYNASIAKCF AADTANQAAT 370 380 390 400 410 NAVQIFGGNG FNSEYPVEKL MRDAKIYQIY EGTSQIQRIV ISRMLLGHFA QNGTSRI

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U42838 Genomic DNA. Translation: AAB52493.1.
PIR	T16838.
RefSeq	NP_510788.1.
UniGene	Cel.378
3D structure databases
HSSP	HSSP built from PDB template 1EGE based on UniProtKB P11310.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:25385N.
IntAct	Q22347. 2 interactions.
STRING	Q22347.
Genome annotation databases
Ensembl	T08G2.3.1; T08G2.3.1; T08G2.3; Caenorhabditis elegans. [Genome view] T08G2.3.2; T08G2.3.2; T08G2.3; Caenorhabditis elegans. [Genome view]
GeneID	181757.
KEGG	cel:T08G2.3.
UCSC	T08G2.3.1. c. elegans.
Organism-specific databases
CTD	181757.
WormBase	WBGene00020366. T08G2.3.
WormPep	T08G2.3. CE07473. [WorfDB]
Phylogenomic databases
OMA	SACCITE.
Enzyme and pathway databases
BRENDA	1.3.99.3. 672.
Gene expression databases
ArrayExpress	Q22347.
Family and domain databases
InterPro	IPR006089. Acyl-CoA_DH_CS. IPR006092. Acyl-CoA_DH_N. IPR006090. Acyl-CoA_Oxase/DH_1. IPR006091. Acyl-CoA_Oxase/DH_M. IPR013786. AcylCoA_DH/ox_N. IPR013764. AcylCoA_oxidase/DH_1/2_C. [Graphical view]
Gene3D	G3DSA:2.40.110.10. Acyl_CoA_DH/ox_M. 1 hit. G3DSA:1.10.540.10. AcylCoA_DH/ox_N. 1 hit. G3DSA:1.20.140.10. AcylCoA_DH_1/2_C. 1 hit.
Pfam	PF00441. Acyl-CoA_dh_1. 1 hit. PF02770. Acyl-CoA_dh_M. 1 hit. PF02771. Acyl-CoA_dh_N. 1 hit. [Graphical view]
PROSITE	PS00072. ACYL_COA_DH_1. 1 hit. PS00073. ACYL_COA_DH_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	915218.

Entry information

Entry name

ACADM_CAEEL

Accession

Primary (citable) accession number: Q22347

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project