Q22295 (UGT50_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative UDP-glucuronosyltransferase ugt-50
Short name=UDPGT 50
EC=2.4.1.17

Gene names

Name:	ugt-50
Synonyms:	ugt16
ORF Names:	T07C5.1

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	523 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the UDP-glycosyltransferase family.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glucuronosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform c (identifier: Q22295-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform b (identifier: Q22295-2)

The sequence of this isoform differs from the canonical sequence as follows:
     182-190: LPTLPSYVP → KPMTTFAES
     191-523: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 523	498	Putative UDP-glucuronosyltransferase ugt-50		PRO_0000036055
Regions
	Transmembrane	490 – 508	19	Helical; Potential
Amino acid modifications
	Glycosylation	84	1	N-linked (GlcNAc...) Ref.2
	Glycosylation	248	1	N-linked (GlcNAc...) Ref.2
	Glycosylation	283	1	N-linked (GlcNAc...) Ref.2
	Glycosylation	487	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	182 – 190	9	LPTLPSYVP → KPMTTFAES in isoform b.		VSP_021638
	Alternative sequence	191 – 523	333	Missing in isoform b.		VSP_021639

Sequences

	Sequence		Length	Mass (Da)
	Isoform c [UniParc]. Last modified November 28, 2006. Version 2. Checksum: 6D00E382BFF26C4D	FASTA	523	60,286
	10 20 30 40 50 60 MHYSQMRWMF FCLTALLHGS FIVNAAKILV YCPSISKSHV LLCSKYADLL HNAGHDTVLF 70 80 90 100 110 120 IPSYSKLLDN YDGAKHAKVW RLHNVTEAYD TKLGTLANVM ENSHIGFIDR LTFDADFWID 130 140 150 160 170 180 MCADLLGKLP EMQHIIDYKF DLVIYNEIDP CTPAIVRLFN IPKTVLLSSE AIMDKVAWNL 190 200 210 220 230 240 GLPTLPSYVP SVEENPNHDR MSFFERMSNV YKFFQSIVVH YLQDIHVLNL FRKEVSSDFP 250 260 270 280 290 300 SIAEIIRNVS LVLVNTDEIF DLPRSYSSKF VYVGMLEAGK DENVTLPKKQ DDYFKKGKSG 310 320 330 340 350 360 SVFVSFGTVT PFRSLPERIQ LSILNAIQKL PDYHFVVKTT ADDESSAQFF STVQNVDLVD 370 380 390 400 410 420 WVPQKAVLRH ANLKLFVSHG GMNSVLETMY YGVPMVIMPV FTDQFRNGRN VERRGAGKMV 430 440 450 460 470 480 LRETVVKETF FDAIHSVLEE KSYSSSVKRI SHLMKNKPFT SEERVTKWID FVLKYETSEH 490 500 510 520 FDLESNNLSI IEHNHLDLFF YLCIISLLNF VVYRKIFKRK SQS
	Isoform b [UniParc]. Checksum: 1032764C783A68BB	FASTA	190	21,650
	10 20 30 40 50 60 MHYSQMRWMF FCLTALLHGS FIVNAAKILV YCPSISKSHV LLCSKYADLL HNAGHDTVLF 70 80 90 100 110 120 IPSYSKLLDN YDGAKHAKVW RLHNVTEAYD TKLGTLANVM ENSHIGFIDR LTFDADFWID 130 140 150 160 170 180 MCADLLGKLP EMQHIIDYKF DLVIYNEIDP CTPAIVRLFN IPKTVLLSSE AIMDKVAWNL 190 GKPMTTFAES

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[2]	"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins." Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T. Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed: 17761667] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-84; ASN-248 AND ASN-283, MASS SPECTROMETRY. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z50006 Genomic DNA. Translation: CAA90301.1. Z50006 Genomic DNA. Translation: CAD44148.1.
PIR	T24647. T24652.
RefSeq	NP_510118.1. NM_077717.2. NP_741913.1. NM_171786.4.
UniGene	Cel.406.
3D structure databases
ProteinModelPortal	Q22295.
SMR	Q22295. Positions 282-456.
ModBase	Search...
Protein-protein interaction databases
STRING	Q22295.
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Proteomic databases
PRIDE	Q22295.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	T07C5.1c; T07C5.1c; T07C5.1.
GeneID	181413.
KEGG	cel:T07C5.1.
NMPDR	fig|6239.3.peg.24902.
UCSC	T07C5.1b. c. elegans.
Organism-specific databases
CTD	181413.
WormBase	T07C5.1b; CE18217; WBGene00011564; ugt-50. T07C5.1c; CE31603; WBGene00011564; ugt-50.
Phylogenomic databases
eggNOG	meNOG13111.
GeneTree	EMGT00070000025718.
HOGENOM	HBG385940.
InParanoid	Q22295.
OMA	SIVVHYL.
PhylomeDB	Q22295.
Gene expression databases
ArrayExpress	Q22295.
Family and domain databases
InterPro	IPR002213. UDP_glucos_trans. [Graphical view]
KO	K00699.
PANTHER	PTHR11926. UDP_glucos_trans. 1 hit.
Pfam	PF00201. UDPGT. 1 hit. [Graphical view]
PROSITE	PS00375. UDPGT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	913828.

Entry information

Entry name

UGT50_CAEEL

Accession

Primary (citable) accession number: Q22295
Secondary accession number(s): O62371, Q8MPX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 28, 2006
Last modified:	November 16, 2011
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project