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Protein

Endoplasmin homolog

Gene

enpl-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Molecular chaperone that functions in the processing and transport of secreted proteins.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei95 – 951ATPBy similarity
Binding sitei137 – 1371ATPBy similarity
Binding sitei150 – 1501ATPBy similarity
Binding sitei187 – 1871ATP; via amide nitrogenBy similarity
Binding sitei426 – 4261ATPCurated

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmin homologImported
Gene namesi
Name:enpl-1Imported
ORF Names:T05E11.3Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiT05E11.3a; CE06362; WBGene00011480; enpl-1.
T05E11.3b; CE45764; WBGene00011480; enpl-1.

Subcellular locationi

  • Endoplasmic reticulum lumen By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

Adults show increased sensitivity to cisplatin, sodium arsenite and zinc. RNAi-mediated knockdown results in induction of the unfolded protein response.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 760737Endoplasmin homologSequence analysisPRO_0000432144Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi95 – 951N-linked (GlcNAc...)PROSITE-ProRule annotation
Glycosylationi423 – 4231N-linked (GlcNAc...)PROSITE-ProRule annotation

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ22235.
PaxDbiQ22235.

2D gel databases

World-2DPAGE0020:Q22235.

Expressioni

Gene expression databases

ExpressionAtlasiQ22235. baseline and differential.

Interactioni

Protein-protein interaction databases

DIPiDIP-24557N.
MINTiMINT-1041118.
STRINGi6239.T05E11.3a.2.

Structurei

3D structure databases

ProteinModelPortaliQ22235.
SMRiQ22235. Positions 57-732.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi757 – 7604Prevents secretion from ERCurated

Sequence similaritiesi

Belongs to the heat shock protein 90 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0020. Eukaryota.
COG0326. LUCA.
GeneTreeiENSGT00840000129758.
HOGENOMiHOG000031988.
InParanoidiQ22235.
KOiK09487.
OMAiKIPKEDY.
PhylomeDBiQ22235.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
HAMAPiMF_00505. HSP90.
InterProiIPR003594. HATPase_C.
IPR019805. Heat_shock_protein_90_CS.
IPR001404. Hsp90_fam.
IPR020575. Hsp90_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view]
PANTHERiPTHR11528. PTHR11528. 1 hit.
PfamiPF02518. HATPase_c. 1 hit.
PF00183. HSP90. 1 hit.
[Graphical view]
PIRSFiPIRSF002583. Hsp90. 1 hit.
PRINTSiPR00775. HEATSHOCK90.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS00298. HSP90. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform aImported (identifier: Q22235-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRFLLVGFVA LLAVSAFIPN VYAEDEIEDA PKETKEETRE EDSIKLDGLS
60 70 80 90 100
VSQIKELRSK AEKHEFQAEV NRMMKLIINS LYRNKEIFLR ELISNASDAL
110 120 130 140 150
DKIRLLSLTD PEQLRETEEM SVKIKADREN RLLHITDTGV GMTRQDLINN
160 170 180 190 200
LGTIARSGTS EFLSKLMDTA TSSDQQQDLI GQFGVGFYAA FLVADRVVVT
210 220 230 240 250
TKNNDDDQYI WESDSASFTI SKDPRGNTLK RGTQITLYLK EEAADFLEPD
260 270 280 290 300
TLKNLVHKYS QFINFDIFLW QSKTEMVEEA VEEEPATTED GAVEEEKEEK
310 320 330 340 350
KTKKVEKTTW DWEKVNNVKP IWMRKPNQVE EDEYKQFYKS ITKDSEEPLS
360 370 380 390 400
HVHFSAEGEV SFRSILYVPK KSPNDMFQNY GKVIENIKLY VRRVFITDDF
410 420 430 440 450
ADMLPKYLSF IRGIVDSDDL PLNVSRENLQ QHKLLKVIKK KLVRKVLDML
460 470 480 490 500
KKLDGAQFDD FWSEFSTNIK LGVMEDPSNR MRLAKLLRFQ SSNDADKTTT
510 520 530 540 550
LAAYVERMKE KQDAIYYMAG TSRKEVETSP FVERLIAKGY EVLFLTEAVD
560 570 580 590 600
EYCIQAMPEY ESKKFQNVAK EGVTIDDGEK AKEAHKGLEE EFKPLTDWLK
610 620 630 640 650
ETALKDLIEK AVVSQRLVKS PSALVASSYG WSGNMERIMK SQAYAKAKDP
660 670 680 690 700
TQDFYATQKK TFEINPRHPV IKELLKRVTA SEEDTTAAST AKLLFETATL
710 720 730 740 750
RSGFSLQDQV GFADRIEAVL RQSLDVSQDA QVETEQHIEE AEPEPEAAEE
760
TTIEEEHSEL
Note: No experimental confirmation available.Curated
Length:760
Mass (Da):87,112
Last modified:November 1, 1996 - v1
Checksum:i9B870D370165F2BD
GO
Isoform bImported (identifier: Q22235-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-72: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:688
Mass (Da):78,921
Checksum:i260282B4F7BACD7B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7272Missing in isoform b. CuratedVSP_057488Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z68751 Genomic DNA. Translation: CAA92973.1.
Z68751 Genomic DNA. Translation: CBZ01798.1.
PIRiT24521.
RefSeqiNP_001255536.1. NM_001268607.1. [Q22235-1]
NP_001255537.1. NM_001268608.1. [Q22235-2]
UniGeneiCel.16874.

Genome annotation databases

EnsemblMetazoaiT05E11.3a.1; T05E11.3a.1; WBGene00011480. [Q22235-1]
T05E11.3a.2; T05E11.3a.2; WBGene00011480. [Q22235-1]
GeneIDi178014.
KEGGicel:CELE_T05E11.3.
UCSCiT05E11.3.1. c. elegans. [Q22235-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z68751 Genomic DNA. Translation: CAA92973.1.
Z68751 Genomic DNA. Translation: CBZ01798.1.
PIRiT24521.
RefSeqiNP_001255536.1. NM_001268607.1. [Q22235-1]
NP_001255537.1. NM_001268608.1. [Q22235-2]
UniGeneiCel.16874.

3D structure databases

ProteinModelPortaliQ22235.
SMRiQ22235. Positions 57-732.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-24557N.
MINTiMINT-1041118.
STRINGi6239.T05E11.3a.2.

2D gel databases

World-2DPAGE0020:Q22235.

Proteomic databases

EPDiQ22235.
PaxDbiQ22235.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT05E11.3a.1; T05E11.3a.1; WBGene00011480. [Q22235-1]
T05E11.3a.2; T05E11.3a.2; WBGene00011480. [Q22235-1]
GeneIDi178014.
KEGGicel:CELE_T05E11.3.
UCSCiT05E11.3.1. c. elegans. [Q22235-1]

Organism-specific databases

CTDi178014.
WormBaseiT05E11.3a; CE06362; WBGene00011480; enpl-1.
T05E11.3b; CE45764; WBGene00011480; enpl-1.

Phylogenomic databases

eggNOGiKOG0020. Eukaryota.
COG0326. LUCA.
GeneTreeiENSGT00840000129758.
HOGENOMiHOG000031988.
InParanoidiQ22235.
KOiK09487.
OMAiKIPKEDY.
PhylomeDBiQ22235.

Miscellaneous databases

NextBioi899362.
PROiQ22235.

Gene expression databases

ExpressionAtlasiQ22235. baseline and differential.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
HAMAPiMF_00505. HSP90.
InterProiIPR003594. HATPase_C.
IPR019805. Heat_shock_protein_90_CS.
IPR001404. Hsp90_fam.
IPR020575. Hsp90_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view]
PANTHERiPTHR11528. PTHR11528. 1 hit.
PfamiPF02518. HATPase_c. 1 hit.
PF00183. HSP90. 1 hit.
[Graphical view]
PIRSFiPIRSF002583. Hsp90. 1 hit.
PRINTSiPR00775. HEATSHOCK90.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS00298. HSP90. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2Imported.
  2. "Depletion of the ER chaperone ENPL-1 sensitizes C. elegans to the anticancer drug cisplatin."
    Natarajan B., Gaur R., Hemmingsson O., Kao G., Naredi P.
    Worm 2:E24059-E24059(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiENPL1_CAEEL
AccessioniPrimary (citable) accession number: Q22235
Secondary accession number(s): E9P859
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 4, 2015
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.