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Protein

Segment polarity protein dishevelled homolog mig-5

Gene

mig-5

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the signal transduction pathways mediated by multiple Wnt genes (PubMed:26795562). Functions redundantly with other dishevelled family members throughout development (PubMed:19298786, PubMed:25344071, PubMed:26460008, PubMed:26795562). During embryonic and larval development, controls cell migration and/or cell fate specification of hypodermal cells, hypodermal seam cells, vulval precursor cells and, through distal tip cell migration, somatic gonad precursor cells (PubMed:16899238, PubMed:26795562). In early embryos, regulates the orientation of the mitotic spindle of blastomeres and specifically, along with dsh-2, is required for the correct mitotic spindle orientation of the ABar blastomere division plane (PubMed:16899238, PubMed:25344071). Controls the polarity and the asymmetric localization of downstream components of the wnt/beta-catenin asymmetry pathway, and in particular, controls the asymmetric localization of the wnt receptor lin-17/Frizzled in ectodermal blast B cells (PubMed:16631156, PubMed:17196955, PubMed:19298786, PubMed:26795562). May act redundantly with dsh-2 to regulate the expression and nuclear localization of the beta-catenin homolog wrm-2, but alone seems to be required for the polarity of wrm-2 during the asymmetric cell division of hypodermal seam cells (PubMed:26795562). Also, maintains the polarity and migration of QL neuroblasts in larvae (PubMed:16899238). During the embryonic development of touch receptor neurons, may act redundantly with dsh-1, downstream of wnt signaling ligands and the wnt receptor lin-17/Frizzled, to direct the growth of neurites of touch receptor neurons towards the anterior of the body of the worm and towards the PLM touch receptor neuron and other tail neurons (PubMed:26460008). May play a role in the guidance of posterior D-type motor neuron axons along the anteroposterior axis (PubMed:19259273).8 Publications

GO - Molecular functioni

GO - Biological processi

  • canonical Wnt signaling pathway Source: GO_Central
  • cell fate determination Source: WormBase
  • cell fate specification involved in pattern specification Source: WormBase
  • cell migration Source: WormBase
  • embryonic digestive tract morphogenesis Source: UniProtKB
  • embryonic morphogenesis Source: WormBase
  • endodermal cell fate specification Source: WormBase
  • epidermis morphogenesis Source: WormBase
  • establishment of mitotic spindle orientation Source: WormBase
  • inductive cell migration Source: WormBase
  • intracellular signal transduction Source: InterPro
  • left/right axis specification Source: UniProtKB
  • neuron migration Source: WormBase
  • regulation of establishment or maintenance of cell polarity Source: WormBase
  • signal transduction involved in regulation of gene expression Source: WormBase
  • Wnt signaling pathway, planar cell polarity pathway Source: WormBase
  • Wnt signaling pathway, regulating spindle positioning Source: WormBase

Keywordsi

Molecular functionDevelopmental protein
Biological processWnt signaling pathway

Enzyme and pathway databases

SignaLinkiQ22227.

Names & Taxonomyi

Protein namesi
Recommended name:
Segment polarity protein dishevelled homolog mig-5Curated
Alternative name(s):
Abnormal cell migration protein 5Imported
Gene namesi
Name:mig-5Imported
ORF Names:T05C12.6Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiT05C12.6a; CE02318; WBGene00003241; mig-5.
T05C12.6b; CE25100; WBGene00003241; mig-5.
T05C12.6c; CE28076; WBGene00003241; mig-5.

Subcellular locationi

GO - Cellular componenti

  • cell cortex Source: WormBase
  • cell junction Source: UniProtKB-SubCell
  • cytoplasm Source: WormBase
  • cytosol Source: GO_Central
  • plasma membrane Source: UniProtKB-SubCell

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Embryonic and larval cell fate, polarity, division and migration defects (PubMed:16899238, PubMed:17196955, PubMed:26795562). In several lineages of the developing gonad 42.6% of hermaphrodites do not have either one or both distal tip cells, which results in the absence of the corresponding gonad arm, and germline proliferation defects in the male germ line (PubMed:16899238). Defects in hypodermal morphogenesis including disorganized dorsal cell intercalation, eventually resulting in 2-fold stage arrest, and failed ventral enclosure in some worms (PubMed:16899238). Cell polarity and migration defects including mitotic spindle misalignment, particularly in the ABar blastomere which results in the posterior cells of the blastomere adopting an alternate more anterior position (PubMed:16899238). Defective QL neuroblast migration with 100% of descendants migrating towards the anterior rather than the posterior of larvae (PubMed:16899238). Disrupted asymmetric cell divisions of hypodermal seam cells with the mislocalization and reduced expression of a wnt/beta catenin pathway component sys-1 and its negative regulator apr-1, and wrm-1 in daughter seam cells (PubMed:26795562). Irregular symmetric localization of lin-17/Frizzled in ectodermal blast B cells (PubMed:17196955).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004374911 – 672Segment polarity protein dishevelled homolog mig-5CuratedAdd BLAST672

Proteomic databases

PaxDbiQ22227.

Expressioni

Developmental stagei

Expressed throughout embryonic and larval development (PubMed:16899238). Expressed in most embryonic cells during hypodermal morphogenesis, and in Z1 and Z4 distal tip precursor cells, in distal tips cells during gonadal migration and in the gonandal primordium, which become vulval precursor cells, during larval development (PubMed:16899238). Also expressed in hypodermal precursor cells P11 and P12 and their daughter cells P11.a, P11.p, P12.a and P12.p, and in the SDQL and PVM neurons which are derived from the QL neuroblast (PubMed:16899238). During larval development, expressed in blast B cells and its descendants, the QL cell and in cells in the nerve ring (PubMed:16631156).2 Publications

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-27038N.
IntActiQ22227. 100 interactors.
MINTiMINT-117775.
STRINGi6239.T05C12.6a.

Structurei

3D structure databases

ProteinModelPortaliQ22227.
SMRiQ22227.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 91DIXPROSITE-ProRule annotationAdd BLAST83
Domaini226 – 294PDZPROSITE-ProRule annotationAdd BLAST69
Domaini427 – 501DEPPROSITE-ProRule annotationAdd BLAST75

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi568 – 571Poly-ArgSequence analysis4

Domaini

The DEP domain is required for cell membrane localization.1 Publication

Sequence similaritiesi

Belongs to the DSH family.Curated

Phylogenomic databases

eggNOGiKOG3571. Eukaryota.
ENOG410Y5G4. LUCA.
GeneTreeiENSGT00390000013552.
HOGENOMiHOG000016057.
InParanoidiQ22227.
OMAiSLWVEST.
OrthoDBiEOG091G041O.
PhylomeDBiQ22227.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR000591. DEP_dom.
IPR001158. DIX.
IPR015506. Dsh/Dvl-rel.
IPR001478. PDZ.
IPR029071. Ubiquitin-rel_dom.
IPR011991. WHTH_DNA-bd_dom.
PANTHERiPTHR10878. PTHR10878. 1 hit.
PfamiView protein in Pfam
PF00610. DEP. 1 hit.
PF00778. DIX. 1 hit.
PF00595. PDZ. 1 hit.
SMARTiView protein in SMART
SM00021. DAX. 1 hit.
SM00049. DEP. 1 hit.
SM00228. PDZ. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiView protein in PROSITE
PS50186. DEP. 1 hit.
PS50841. DIX. 1 hit.
PS50106. PDZ. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform aImported (identifier: Q22227-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPPCTSDCS QIKVFYYLDD ETTPYVSVIE AREGVATLGN FKNSFTKRGY
60 70 80 90 100
KYYAKELDPD IQREVKVELT TDSDRLRKSQ NGFYEIFLVS TPGYGTLPRN
110 120 130 140 150
SGTMTRPQRT ALDKRRRRSA DFDATPYSDA SLAPSTIVSR RAGEHLAELY
160 170 180 190 200
TSNSEDPYQY DEHTRRTGDD SSLYEPLAAR DMNKIYDDDR RRKKQKKERF
210 220 230 240 250
RRPYVPSTIS SATESSVNSG LPRILEIYLP MKNVPYLGLS VCTIDGHIFV
260 270 280 290 300
SEIAPEGAVE KDGRVNVGDQ ILQVNRVSFE ELSGPQAVRS LREAASSKRP
310 320 330 340 350
ITLYISKFAR GAPSEYDDPL ASMASETMPL DVGVWVETAV QNTEKMKALG
360 370 380 390 400
LDPQEQTATT IDDGTLPFTS TASDDEERML YDQRRNGIPR ALIEEAERKR
410 420 430 440 450
ENEQNEKIEQ LTEMIDPIIV VRSMARPDSG LAVKNRKWLK ILVPMSFIGR
460 470 480 490 500
DLVDWLVDHM ADIHNRKKAR IYAARLLAAG LIRHVVSKLT FTEKCYYVFG
510 520 530 540 550
DGILGNDRNS TDTTGTSGTT MRVEATTEVT YVGSPAPHAL AARVGRNIPH
560 570 580 590 600
RLETTTLSPV AHDQTWLRRR RDCESPMTND YASMVGESQI GMNPVGNYHV
610 620 630 640 650
FGTKNNHRQV PAPSQVTSSS LTNGSGGLGG PPPTPLSSTM VLAASPIQSQ
660 670
NAVNHDFDGE NSSNSRTRIL RT
Length:672
Mass (Da):75,018
Last modified:November 1, 1996 - v1
Checksum:i198FA92312997CF6
GO
Isoform bImported (identifier: Q22227-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     624-672: GSGGLGGPPP...SNSRTRILRT → VTNSRKWWLR...SQPRFRRGEQ

Show »
Length:666
Mass (Da):75,091
Checksum:i21C4EB2D673ACD1F
GO
Isoform cImported (identifier: Q22227-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     300-340: Missing.
     624-672: GSGGLGGPPP...SNSRTRILRT → VTNSRKWWLR...SQPRFRRGEQ

Show »
Length:625
Mass (Da):70,680
Checksum:i63FB8132B1E25CC2
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_058543300 – 340Missing in isoform c. CuratedAdd BLAST41
Alternative sequenceiVSP_058544624 – 672GSGGL…RILRT → VTNSRKWWLRRPSADSSIQY YGSSSFSDSVAERSQPRFRR GEQ in isoform b and isoform c. CuratedAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063244 mRNA. Translation: AAC16434.1.
AF070920 mRNA. Translation: AAC24231.1.
BX284602 Genomic DNA. Translation: CAA91307.1.
BX284602 Genomic DNA. Translation: CAB61022.1.
BX284602 Genomic DNA. Translation: CAC42334.1.
PIRiT24507.
T43171.
T43211.
RefSeqiNP_001022316.1. NM_001027145.2. [Q22227-1]
NP_001022317.1. NM_001027146.2. [Q22227-2]
NP_001022318.1. NM_001027147.2. [Q22227-3]
UniGeneiCel.18251.

Genome annotation databases

EnsemblMetazoaiT05C12.6a; T05C12.6a; WBGene00003241. [Q22227-1]
T05C12.6b; T05C12.6b; WBGene00003241. [Q22227-2]
T05C12.6c; T05C12.6c; WBGene00003241. [Q22227-3]
GeneIDi174317.
KEGGicel:CELE_T05C12.6.
UCSCiT05C12.6b. c. elegans.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063244 mRNA. Translation: AAC16434.1.
AF070920 mRNA. Translation: AAC24231.1.
BX284602 Genomic DNA. Translation: CAA91307.1.
BX284602 Genomic DNA. Translation: CAB61022.1.
BX284602 Genomic DNA. Translation: CAC42334.1.
PIRiT24507.
T43171.
T43211.
RefSeqiNP_001022316.1. NM_001027145.2. [Q22227-1]
NP_001022317.1. NM_001027146.2. [Q22227-2]
NP_001022318.1. NM_001027147.2. [Q22227-3]
UniGeneiCel.18251.

3D structure databases

ProteinModelPortaliQ22227.
SMRiQ22227.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-27038N.
IntActiQ22227. 100 interactors.
MINTiMINT-117775.
STRINGi6239.T05C12.6a.

Proteomic databases

PaxDbiQ22227.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT05C12.6a; T05C12.6a; WBGene00003241. [Q22227-1]
T05C12.6b; T05C12.6b; WBGene00003241. [Q22227-2]
T05C12.6c; T05C12.6c; WBGene00003241. [Q22227-3]
GeneIDi174317.
KEGGicel:CELE_T05C12.6.
UCSCiT05C12.6b. c. elegans.

Organism-specific databases

CTDi174317.
WormBaseiT05C12.6a; CE02318; WBGene00003241; mig-5.
T05C12.6b; CE25100; WBGene00003241; mig-5.
T05C12.6c; CE28076; WBGene00003241; mig-5.

Phylogenomic databases

eggNOGiKOG3571. Eukaryota.
ENOG410Y5G4. LUCA.
GeneTreeiENSGT00390000013552.
HOGENOMiHOG000016057.
InParanoidiQ22227.
OMAiSLWVEST.
OrthoDBiEOG091G041O.
PhylomeDBiQ22227.

Enzyme and pathway databases

SignaLinkiQ22227.

Miscellaneous databases

PROiPR:Q22227.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR000591. DEP_dom.
IPR001158. DIX.
IPR015506. Dsh/Dvl-rel.
IPR001478. PDZ.
IPR029071. Ubiquitin-rel_dom.
IPR011991. WHTH_DNA-bd_dom.
PANTHERiPTHR10878. PTHR10878. 1 hit.
PfamiView protein in Pfam
PF00610. DEP. 1 hit.
PF00778. DIX. 1 hit.
PF00595. PDZ. 1 hit.
SMARTiView protein in SMART
SM00021. DAX. 1 hit.
SM00049. DEP. 1 hit.
SM00228. PDZ. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiView protein in PROSITE
PS50186. DEP. 1 hit.
PS50841. DIX. 1 hit.
PS50106. PDZ. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMIG5_CAEEL
AccessioniPrimary (citable) accession number: Q22227
Secondary accession number(s): G5EC49, O76471, Q95ZQ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2016
Last sequence update: November 1, 1996
Last modified: May 10, 2017
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.