Reviewed, UniProtKB/Swiss-Prot Q221I1 (CHEB1_RHOFD)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase 1
    EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	Rfer_0571

Organism

Rhodoferax ferrireducens (strain DSM 15236 / ATCC BAA-621 / T118) [Complete proteome] [HAMAP]

Taxonomic identifier

338969 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Rhodoferax

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity.
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm. HAMAP MF_00099
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity.
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 373	373	Chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000264304
Regions
	Domain	16 – 133	118	Response regulatory
	Domain	175 – 367	193	CheB-type methylesterase
Sites
	Active site	187	1	By similarity
	Active site	213	1	By similarity
	Active site	309	1	By similarity
Amino acid modifications
	Modified residue	67	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q221I1-1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: A8C006434395A54F	FASTA	373	39,828
	10 20 30 40 50 60 MARWICFEDI ELKKIRVVVV DDSALVRSLL AEIINRQKDM ECVGTANDPL IAREMIRELN 70 80 90 100 110 120 PDVLTLDIEM PRMDGIDFLG RLMRLRPMPV VMVSTLTERG AEITMKALEL GAIDFVAKPR 130 140 150 160 170 180 IGVASGLTEL SDQIVEKVRI AATAHVRRAP AVPAAATPAV GGAVGGHAPV ASIGRVSTEK 190 200 210 220 230 240 LICIGASTGG TEAIKEILTR LPADSPGIVI TQHMPPGFTT SFAARLNSLC QIAVQEAVNG 250 260 270 280 290 300 ERILPGHAYI APGGKQFRVS RSGANYVALV EDGELVNRHK PSVEVLFKSV ASVVGRNAYG 310 320 330 340 350 360 VMLTGMGNDG AKAMREMKDA GSYNFVQDEA SCIVFGMPRE AILHGAADEV LPLTAIAPAL 370 IAKLSSATDR YRV

References

[1]

"Complete sequence of chromosome of Rhodoferax ferrireducens DSM 15236." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Ivanova N., Richardson P. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000267 Genomic DNA. Translation: ABD68322.1.
RefSeq	YP_521853.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q221I1.
Genome annotation databases
GeneID	3960720.
GenomeReviews	Gene locus Rfer_0571 in contig CP000267_GR.
KEGG	rfr:Rfer_0571.
NMPDR	fig|338969.3.peg.889.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q221I1.
OMA	IVIAQHI.
Enzyme and pathway databases
BioCyc	RFER338969:RFER_0571-MON.
Family and domain databases
HAMAP	MF_00099. [Tree]
InterPro	IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
ProDom	PD005328. CheB_methylest. 1 hit. PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_RHOFD

Accession

Primary (citable) accession number: Q221I1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	April 18, 2006
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)