Q22197 (CKI1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase inhibitor 1

Gene names

Name:	cki-1
ORF Names:	T05A6.1

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	184 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Negative cell-cycle regulator that functions at the G1-to-S-phase transition. It is required for suspension of cell cycling in dauer larvae and starved L1 larvae. In vulval precursor cells (VPCs) a pathway of heterochronic genes acts via cki-1 to maintain VPC in G1 during L2 larval stage. Cul-2 may function in ubiquitin-mediated degradation by targeting cki-1 for degradation. Ref.1 Ref.3
Subcellular location	Nucleus Ref.1.
Tissue specificity	In embryos, expression is first seen in pharyngeal primordium and later in all differentiating cells. Post embryonic expression corresponds to developmental patterns of cell cycle progression in many tissues including sex myoblasts, distal tip cells, vulval cells, seam cells, neurons, intestine cells and hypodermal cells. Ref.1 Ref.3
Sequence similarities	Belongs to the CDI family.

Ontologies

Keywords
Biological process	Cell cycle Ubl conjugation pathway
Cellular component	Nucleus
Molecular function	Protein kinase inhibitor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	body morphogenesis Inferred from mutant phenotype Ref.3. Source: WormBase cell division Inferred from mutant phenotype Ref.3. Source: WormBase embryo development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase germline cell cycle switching, mitotic to meiotic cell cycle Inferred from mutant phenotype Ref.3. Source: WormBase gonad development Inferred from mutant phenotype Ref.3. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype Ref.3. Source: WormBase mitotic cell cycle arrest Inferred from mutant phenotype Ref.3. Source: WormBase molting cycle, collagen and cuticulin-based cuticle Inferred from mutant phenotype Ref.3. Source: WormBase negative regulation of multicellular organism growth Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase oviposition Inferred from mutant phenotype. Source: WormBase regulation of cell proliferation Inferred from mutant phenotype. Source: WormBase regulation of meiosis Inferred from mutant phenotype. Source: WormBase
Cellular component	nucleus Inferred from direct assay. Source: WormBase
Molecular function	cyclin-dependent protein kinase inhibitor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 184	184	Cyclin-dependent kinase inhibitor 1		PRO_0000190089

Sequences

	Sequence		Length	Mass (Da)
	Q22197 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 184D794528E2AAE2	FASTA	184	21,245
	10 20 30 40 50 60 MSSARRCLFG RPTPEQRSRT RIWLEDAVKR MRQEESQKWG FDFELETPLP SSAGFVYEVI 70 80 90 100 110 120 PENCVPEFYR TKVLTVRTTC SSLDISSTTL TPLSSPSTSD KEEPSLMDPN SSFEDEEEPK 130 140 150 160 170 180 KWQFREPPTP RKTPTKRQQK MTDFMAVSRK KNSLSPNKLS PVNVIFTPKS RRPTIRTRSS CSPY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CUL-2 is required for the G1-to-S-phase transition and mitotic chromosome condensation in Caenorhabditis elegans." Feng H., Zhong W., Punkosdy G., Gu S., Zhou L., Seabolt E.K., Kipreos E.T. Nat. Cell Biol. 1:486-492(1999) [PubMed: 10587644] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"Developmental regulation of a cyclin-dependent kinase inhibitor controls postembryonic cell cycle progression in Caenorhabditis elegans." Hong Y., Roy R., Ambros V. Development 125:3585-3597(1998) [PubMed: 9716524] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF179358 mRNA. Translation: AAF13868.1. Z50796 Genomic DNA. Translation: CAA90667.1.
PIR	T24496.
RefSeq	NP_495641.1. NM_063240.3.
UniGene	Cel.19541.
3D structure databases
ProteinModelPortal	Q22197.
SMR	Q22197. Positions 2-71.
ModBase	Search...
Protein-protein interaction databases
STRING	Q22197.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	T05A6.1; T05A6.1; T05A6.1.
GeneID	174260.
KEGG	cel:T05A6.1.
UCSC	T05A6.1. c. elegans.
Organism-specific databases
CTD	174260.
WormBase	T05A6.1; CE03628; WBGene00000516; cki-1.
Phylogenomic databases
eggNOG	KOG4743.
GeneTree	EMGT00050000017789.
HOGENOM	HBG381294.
InParanoid	Q22197.
OMA	REPPTPR.
PhylomeDB	Q22197.
Gene expression databases
ArrayExpress	Q22197.
Family and domain databases
InterPro	IPR003175. CDI. [Graphical view]
PANTHER	PTHR10265. CDI. 1 hit.
Pfam	PF02234. CDI. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	883269.

Entry information

Entry name

CKI1_CAEEL

Accession

Primary (citable) accession number: Q22197

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2003
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project