Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Fer-related kinase 1

Gene

frk-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Non-receptor tyrosine-protein kinase which plays a role in morphogenesis by regulating the epidermal enclosure of the embryo, independently of its kinase activity (PubMed:15958510). Prevents hyperactivation of the Wnt signaling pathway during endoderm development, probably by preventing hmp-2 nuclear translocation (PubMed:20805471).2 Publications

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.1 Publication

Cofactori

Mn2+1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei161 – 1611ATPPROSITE-ProRule annotation
Active sitei252 – 2521Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi137 – 1459ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • beta-catenin binding Source: WormBase
  • metal ion binding Source: UniProtKB-KW
  • non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  • protein kinase activity Source: WormBase

GO - Biological processi

  • embryonic body morphogenesis Source: WormBase
  • negative regulation of catenin import into nucleus Source: UniProtKB
  • negative regulation of cell division Source: UniProtKB
  • negative regulation of Wnt signaling pathway involved in digestive tract morphogenesis Source: UniProtKB
  • positive regulation of cell proliferation Source: WormBase
  • protein autophosphorylation Source: WormBase
  • regulation of epidermal cell differentiation Source: WormBase
  • regulation of protein localization Source: WormBase
  • single organismal cell-cell adhesion Source: WormBase
  • Wnt signaling pathway involved in digestive tract morphogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiQ22146.

Names & Taxonomyi

Protein namesi
Recommended name:
Fer-related kinase 11 Publication (EC:2.7.10.21 Publication)
Alternative name(s):
Fer oncogene-related kinase 1Imported
Gene namesi
Name:frk-1Imported
ORF Names:T04B2.2Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiT04B2.2a; CE03609; WBGene00001487; frk-1.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication
  • Cell junction 1 Publication
  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication

  • Note: Localizes to the nucleus during cell division in the early embryo and in seam cells and in the developing germline at the larval stage. Localizes in the cytoplasm and at the cell-cell contacts during embryo enclosure and elongation. Nuclear exclusion during elongation is regulated by integrin alpha subunit pat-3 and beta-catenin hmp-2.1 Publication

GO - Cellular componenti

  • cell-cell junction Source: WormBase
  • cytoplasm Source: WormBase
  • nucleus Source: WormBase
  • plasma membrane Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown causes a disruption in the dorsal intercalation and ventral migration of epidermal cells, preventing enclosure and elongation of the embryo. Epidermal cells tend to round up and appear to be defective in adhesion (PubMed:15958510). In addition, causes partial nuclear re-localization of hmp-2 in the embryonic epidermis and the production of supernumerary gut nuclei probably resulting from epithelial cell hyperproliferation (PubMed:20805471).2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi308 – 3081D → R: Loss of kinase activity. No defect in embryo enclosure and elongation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 390390Fer-related kinase 1CuratedPRO_0000433994Add
BLAST

Proteomic databases

PaxDbiQ22146.

Expressioni

Developmental stagei

Expressed in early embryo and, during later stages of embryogenesis, in epithelial cells, body wall muscle, germline and mature sperm.1 Publication

Interactioni

Subunit structurei

Interacts with hmp-2.1 Publication

GO - Molecular functioni

  • beta-catenin binding Source: WormBase

Protein-protein interaction databases

STRINGi6239.T04B2.2b.

Structurei

3D structure databases

ProteinModelPortaliQ22146.
SMRiQ22146. Positions 16-383.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 11997SH2PROSITE-ProRule annotationAdd
BLAST
Domaini131 – 386256Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiKOG0194. Eukaryota.
ENOG410Y6RP. LUCA.
GeneTreeiENSGT00760000119011.
HOGENOMiHOG000233858.
PhylomeDBiQ22146.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q22146-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTVEKSSNN DASVTDDIRS AEYYHGMVPR QDAEGFLKRE GDFLVRKTEQ
60 70 80 90 100
MPGKVVLAMS VRVTDELCRH FMLNMDPTSN KFYFEHTHQE STISELINWH
110 120 130 140 150
MTTKTPISAA SGAKIRRPME RSPWLINHDS IVANKKLGEG AFGDVFIAEL
160 170 180 190 200
DQGGKQEVAV KTMRAEATRE ARLRFMKEAR LMRKYQHKHV VKLIGVAIHE
210 220 230 240 250
HPLMIVMEYC PNGSLLSHLK KNKVSLIEKL RFTTEAADGI AYLERSKCIH
260 270 280 290 300
RDIAARNCLL SAKNELKISD FGMSDNKDEI KDETLEKVPI KWLAPETMQE
310 320 330 340 350
KVYTHKTDIW TFGVLVWEIY SDGAEPYPGL TKIQTRAKIV VNDYRMKMPD
360 370 380 390
GTHPTVADVV TGTCWQKNPE KRSTMDSIHK KLREFYESKK
Length:390
Mass (Da):44,692
Last modified:November 1, 1996 - v1
Checksum:i8371D488D5C7FEC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284604 Genomic DNA. Translation: CAA92609.1.
PIRiT24437.
RefSeqiNP_001255459.1. NM_001268530.1.
UniGeneiCel.57.

Genome annotation databases

EnsemblMetazoaiT04B2.2a; T04B2.2a; WBGene00001487.
GeneIDi191635.
KEGGicel:CELE_T04B2.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284604 Genomic DNA. Translation: CAA92609.1.
PIRiT24437.
RefSeqiNP_001255459.1. NM_001268530.1.
UniGeneiCel.57.

3D structure databases

ProteinModelPortaliQ22146.
SMRiQ22146. Positions 16-383.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.T04B2.2b.

Proteomic databases

PaxDbiQ22146.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT04B2.2a; T04B2.2a; WBGene00001487.
GeneIDi191635.
KEGGicel:CELE_T04B2.2.

Organism-specific databases

CTDi191635.
WormBaseiT04B2.2a; CE03609; WBGene00001487; frk-1.

Phylogenomic databases

eggNOGiKOG0194. Eukaryota.
ENOG410Y6RP. LUCA.
GeneTreeiENSGT00760000119011.
HOGENOMiHOG000233858.
PhylomeDBiQ22146.

Enzyme and pathway databases

SignaLinkiQ22146.

Miscellaneous databases

PROiQ22146.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2Imported.
  2. "Essential kinase-independent role of a Fer-like non-receptor tyrosine kinase in Caenorhabditis elegans morphogenesis."
    Putzke A.P., Hikita S.T., Clegg D.O., Rothman J.H.
    Development 132:3185-3195(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, INTERACTION WITH HMP-2, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, MUTAGENESIS OF ASP-308.
  3. "Repression of Wnt signaling by a Fer-type nonreceptor tyrosine kinase."
    Putzke A.P., Rothman J.H.
    Proc. Natl. Acad. Sci. U.S.A. 107:16154-16159(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiFRK1_CAEEL
AccessioniPrimary (citable) accession number: Q22146
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2015
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.