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Q220P5 (SYL_RHOFD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Rfer_0758
OrganismRhodoferax ferrireducens (strain DSM 15236 / ATCC BAA-621 / T118) [Complete proteome] [HAMAP]
Taxonomic identifier338969 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeAlbidiferax

Protein attributes

Sequence length892 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 892892Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009408

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif640 – 6445"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6431ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q220P5 [UniParc].

Last modified April 18, 2006. Version 1.
Checksum: 1C6F17513F50E8A6

FASTA89299,291
        10         20         30         40         50         60 
MQEKYTPLDV EQAAQSHWTA MDAYRVTEDS SKKKFYACSM LPYPSGKLHM GHVRNYTIND 

        70         80         90        100        110        120 
MLTRYLRMNG YNVLMPMGWD AFGLPAENAA LKNGVPPAQW TFENIAYMKQ QMQAMGLAID 

       130        140        150        160        170        180 
WSREVATCDP SYYKWNQWLF LKMLDKGIAY RKTQVVNWDP VDQTVLANEQ VIDGKGWRTG 

       190        200        210        220        230        240 
ATVEKREIPG YYLKITDYAE ELLGQVQTGL PGWPERVKLM QENWIGKSEG VRFAFTHDIR 

       250        260        270        280        290        300 
DASGALIDDG RMYVFTTRPD TIKGVTFCAV APEHPLALHA ARGNPALAAF IEECKTGGTT 

       310        320        330        340        350        360 
EAELATQEKK GLDTGLIVTH PLTQLPVAVW VGNYVLMSYG DGAVMGVPAH DERDFAFAKK 

       370        380        390        400        410        420 
YGLPIKQVVQ VDGEQFDYDQ WQDWYADKQR GVAINSGSFS GMSFKQAVEA VAHKLAALGL 

       430        440        450        460        470        480 
GEKKTTWRLR DWGVSRQRYW GTPIPIIHCP EHGAVPVPEK DLPVVLPQDC VPDGSGNPLH 

       490        500        510        520        530        540 
KHEGFHAGVV CPICGVAARR ETDTMDTFVD SSWYFMRYCD PKNEKAMVGE GAAYWMPMDQ 

       550        560        570        580        590        600 
YIGGIEHAIL HLLYARFWTK VMRDLGLVKV DEPFTKLLTQ GMVLNHIYSR KGENGAKDYF 

       610        620        630        640        650        660 
WPNDVENVSD ETGKVVAAKL KVAVGNLPAG TLVDYEGIGT MSKSKNNGVD PQDLIERYGA 

       670        680        690        700        710        720 
DTARLYTMFT APPEATLEWN DAAVEGSYRF LRRVWNFGIK LSTIDMIAVD ESTTGATALN 

       730        740        750        760        770        780 
DINFSKEAKV LRLEIHTVLK QVDYDYQRMQ YNTVVSGAMK MINALENFKA TDSGGAQVAL 

       790        800        810        820        830        840 
IEGFGILLRC LYPATPHIAH SLWQGLGYAG TLGDLLDAPW PQVDELALLQ DEVELMLQIN 

       850        860        870        880        890 
GKLRGAIVVS AGASKVEIEL AAIDSEVFRK LANGATPKKV IVVPGRLVNL VV 

« Hide

References

[1]"Complete sequence of chromosome of Rhodoferax ferrireducens DSM 15236."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. expand/collapse author list , Kyrpides N., Ivanova N., Richardson P.
Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 15236 / ATCC BAA-621 / T118.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000267 Genomic DNA. Translation: ABD68508.1.
RefSeqYP_522039.1. NC_007908.1.

3D structure databases

ProteinModelPortalQ220P5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING338969.Rfer_0758.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD68508; ABD68508; Rfer_0758.
GeneID3964136.
KEGGrfr:Rfer_0758.
PATRIC23234029. VBIRhoFer131161_1039.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycAFER338969:GHU9-761-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_RHOFD
AccessionPrimary (citable) accession number: Q220P5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 18, 2006
Last modified: April 16, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries