Q220P2 (PROA_RHOFD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Rfer_0761

Organism

Rhodoferax ferrireducens (strain DSM 15236 / ATCC BAA-621 / T118) [Complete proteome] [HAMAP]

Taxonomic identifier

338969 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Albidiferax

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000252587

Sequences

	Sequence		Length	Mass (Da)
	Q220P2 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: 29E355694790C5D6	FASTA	444	47,463
	10 20 30 40 50 60 MIPLNTTDYA NALGLKAKQA SALMARAPTA TKNAALRKLA GLLRANVNAL QIDNARDIER 70 80 90 100 110 120 AVQSGLAAPM VDRLKLSPQV IETCAQGCEQ LAGMADVIGE IIGMKQQPSG IRVGQMRVPI 130 140 150 160 170 180 GVFGMIFESR PNVTIEAASL SIKSGNACIL RGGSEAIESN KALARLVQQA LTESGLPAEA 190 200 210 220 230 240 VQLVQTTDRA VVGQLITMPQ YVDVIIPRGG KGLIERISRD AKVPVIKHLD GNCHTYVDDP 250 260 270 280 290 300 CDIAMAVKVA DNAKTNKYSP CNATESLLVA RAVAAEFLPS IGRVYAAKGV EMRCDPQALA 310 320 330 340 350 360 IFKENQPVAP VNIGHDAIDS AVELKPVLVL AQESDWFEEY LAPIISIKVV AGVDEAIAHI 370 380 390 400 410 420 NRYSSHHTDA ILTRDHMHAQ QFLREVDSAS VMVNTSTRFA DGFEYGLGAE IGISTDKFHA 430 440 RGPVGIEGLT SLKYVVLGDG EIRA

References

[1]

"Complete sequence of chromosome of Rhodoferax ferrireducens DSM 15236." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Ivanova N., Richardson P. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 15236 / ATCC BAA-621 / T118.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000267 Genomic DNA. Translation: ABD68511.1.
RefSeq	YP_522042.1. NC_007908.1.
3D structure databases
ProteinModelPortal	Q220P2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q220P2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3964139.
GenomeReviews	Gene locus Rfer_0761 in contig CP000267_GR.
KEGG	rfr:Rfer_0761.
NMPDR	fig|338969.3.peg.971.
PATRIC	23234039. VBIRhoFer131161_1044.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0014.
HOGENOM	HBG318080.
OMA	YGICGAM.
PhylomeDB	Q220P2.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	RFER338969:RFER_0761-MONOMER.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 2 hits. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_RHOFD

Accession

Primary (citable) accession number: Q220P2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	April 18, 2006
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program