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Protein

F-box/WD repeat-containing protein mec-15

Gene

mec-15

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in mechanosensory transduction (touch sensitivity), touch receptor neuron development and synapse formation (PubMed:8692859, PubMed:19652181). Regulates expression of the protein snb-1 and the distribution of synaptic vesicles at synapses to promote synaptic transmission at the neuromuscular junctions of GABAergic motor neurons (PubMed:23527112).3 Publications

GO - Biological processi

  • detection of mechanical stimulus involved in sensory perception of touch Source: UniProtKB
  • mechanosensory behavior Source: UniProtKB
  • neuron development Source: WormBase
  • positive regulation of detection of mechanical stimulus involved in sensory perception of touch Source: UniProtKB
  • positive regulation of inhibitory postsynaptic potential Source: UniProtKB
  • positive regulation of mechanosensory behavior Source: UniProtKB
  • positive regulation of muscle contraction Source: UniProtKB
  • positive regulation of synaptic transmission, GABAergic Source: UniProtKB
  • positive regulation of synaptic vesicle transport Source: WormBase
  • synaptic vesicle targeting Source: UniProtKB

Enzyme and pathway databases

ReactomeiR-CEL-8951664 Neddylation
R-CEL-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiQ22071

Names & Taxonomyi

Protein namesi
Recommended name:
F-box/WD repeat-containing protein mec-15Curated
Alternative name(s):
Mechanosensory abnormality protein 15Imported
Gene namesi
Name:mec-15Imported
ORF Names:T01E8.4Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiT01E8.4 ; CE02308 ; WBGene00003177 ; mec-15

Subcellular locationi

Pathology & Biotechi

Disruption phenotypei

Temperature-sensitive phenotype with defective touch receptor neuron synaptic transmission conferring variable degrees of touch insensitivity amongst individual animals (PubMed:8692859, PubMed:19652181). Touch receptor neurons and GABAergic motor neurons have mis-localized presynaptic vesicles as indicated by aberrant expression of the protein snb-1 and distorted cell bodies (PubMed:19652181, PubMed:23527112). Reduced rate of inhibitory postsynaptic currents at the neuromuscular junctions of GABAergic motor neurons (PubMed:23527112). No effects on cholinergic synaptic transmission or snb-1 distribution in cholinergic motor neurons (PubMed:23527112). Increased rate of paralysis, in response to acetylcholine esterase inhibition by the drug Aldicarb, due to increased accumulation of acetylcholine in the extracellular fluid resulting permanent contraction of body wall muscles (PubMed:23527112). Knockout with a mec-7 heterozygous mutant, but not a null mec-7 mutant, rescues the touch sensitivity defect in the mec-15 single mutant (PubMed:19652181).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004422061 – 406F-box/WD repeat-containing protein mec-15CuratedAdd BLAST406

Proteomic databases

EPDiQ22071
PaxDbiQ22071
PeptideAtlasiQ22071

Expressioni

Tissue specificityi

Expressed in several neurons in the head, tail and ventral cord, but absent in touch receptor neurons in adults (PubMed:19652181, PubMed:23527112). Expressed in GABAergic and cholinergic motor neurons (PubMed:23527112).2 Publications

Developmental stagei

Expressed in touch receptor neurons and several neurons in the head, tail and ventral cord during larval development.1 Publication

Gene expression databases

BgeeiWBGene00003177

Interactioni

Subunit structurei

May interact with the SCF ubiquitin ligase complex component skr-1.1 Publication

Protein-protein interaction databases

STRINGi6239.T01E8.4.1

Structurei

3D structure databases

ProteinModelPortaliQ22071
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 53F-boxPROSITE-ProRule annotationAdd BLAST48
Repeati101 – 142WD 1Sequence analysisAdd BLAST42
Repeati156 – 195WD 2Sequence analysisAdd BLAST40
Repeati242 – 279WD 3Sequence analysisAdd BLAST38
Repeati281 – 320WD 4Sequence analysisAdd BLAST40
Repeati365 – 406WD 5Sequence analysisAdd BLAST42

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410IQD3 Eukaryota
ENOG4111G8F LUCA
GeneTreeiENSGT00390000006806
HOGENOMiHOG000021824
InParanoidiQ22071
KOiK10265
OMAiWCASHEL
OrthoDBiEOG091G0AVG
PhylomeDBiQ22071

Family and domain databases

Gene3Di2.130.10.10, 2 hits
InterProiView protein in InterPro
IPR036047 F-box-like_dom_sf
IPR001810 F-box_dom
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF12937 F-box-like, 1 hit
PF00400 WD40, 1 hit
SMARTiView protein in SMART
SM00256 FBOX, 1 hit
SM00320 WD40, 4 hits
SUPFAMiSSF50978 SSF50978, 1 hit
SSF81383 SSF81383, 1 hit
PROSITEiView protein in PROSITE
PS50181 FBOX, 1 hit
PS50082 WD_REPEATS_2, 1 hit
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Q22071-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNAQPTELI SLPSELLCHL FTYLPQRQLI TEIPLVCRRF NTILNDDKFW
60 70 80 90 100
SRRIRTEQKV RLPDCELKHP EYEPKKSFYA MHRQRDRWRA WETQTVITAP
110 120 130 140 150
GHSATVDSVM LFENNNKQFC LSGSRDRTIR LWSIENARNG EDTEQNPWTV
160 170 180 190 200
AKDDTAHSGW IWNMAQESTS TNFYTTSWDS TVKSWHITDN GALQNLNSVN
210 220 230 240 250
VGSAAQCISC SGNENEVVCT TFAKRVAVID SRTFQVVADH KLHKRAVIAL
260 270 280 290 300
AVQGDKIFTS GEDRLMMMVD RRNFSKPVLF EYSPTAYKSC LSLQCNQLLT
310 320 330 340 350
STSDGKVKLY DANNFNVLQT YSVGSYTRQS FLEHGAHLIM ARSLKRNYTF
360 370 380 390 400
SIYSPGTRSQ QWCASHELAA EPAKFDYSPS SRTLAIGNGD SSILFCLPAS

APDQEN
Length:406
Mass (Da):46,173
Last modified:November 1, 1996 - v1
Checksum:iD3B5F68B370C6F0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284602 Genomic DNA Translation: CAA88747.1
PIRiT24301
RefSeqiNP_496207.1, NM_063806.6
UniGeneiCel.14496

Genome annotation databases

EnsemblMetazoaiT01E8.4; T01E8.4; WBGene00003177
GeneIDi174587
KEGGicel:CELE_T01E8.4
UCSCiT01E8.4 c. elegans

Similar proteinsi

Entry informationi

Entry nameiMEC15_CAEEL
AccessioniPrimary (citable) accession number: Q22071
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2017
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health