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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma plc-3

Gene

plc-3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) which plays an important role in the regulation of intracellular signaling cascades (Probable). Regulates basal and ovulatory sheath cell contractions by controlling Ca2+ oscillations via IP3-mediated activation of IP3 receptor itr-1 (PubMed:15194811). In intestinal epithelial cells, regulates Ca2+ oscillations which control posterior body wall muscle contractions required for defecation by IP3-mediated activation of itr-1 and probably by activating TRPM channels gon-2 and gtl-1 by reducing PIP2 levels (PubMed:19923421, PubMed:16186564). By activating tpa-1 via DAG production, required for the expression of antimicrobial peptide nlp-29 in the epidermis in response to fungal infection or physical injury (PubMed:19380113). By triggering Ca2+ transient via IP3-mediated activation of IPR3 receptor itr-1 in ASH sensory neurons, involved in avoidance behavior in response to nose touch (PubMed:19730689). Probably by regulating neuronal transmission in ALA neurons, mediates the decrease in pharyngeal pumping and locomotion during the quiescent state that precedes each larval molt, downstream of lin-3 and receptor let-23 and upstream of tpa-1 but not itr-1 (PubMed:17891142). During embryogenesis, may play an role in epidermal morphogenesis together with plc-1 (PubMed:18369461). Probably downstream of receptor daf-2, regulates male-sex muscle excitability in the absence of food (PubMed:18604269).Curated8 Publications

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.UniRule annotation

Cofactori

Ca2+Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei367PROSITE-ProRule annotation1
Active sitei419PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • intracellular signal transduction Source: InterPro
  • phospholipid catabolic process Source: InterPro
  • sleep Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-CEL-114604. GPVI-mediated activation cascade.
R-CEL-1169408. ISG15 antiviral mechanism.
R-CEL-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-CEL-202433. Generation of second messenger molecules.
R-CEL-5607764. CLEC7A (Dectin-1) signaling.
R-CEL-5654219. Phospholipase C-mediated cascade: FGFR1.
R-CEL-5654221. Phospholipase C-mediated cascade, FGFR2.
R-CEL-5654227. Phospholipase C-mediated cascade, FGFR3.
R-CEL-5654228. Phospholipase C-mediated cascade, FGFR4.
SignaLinkiQ22070.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma plc-3Curated (EC:3.1.4.11UniRule annotation)
Alternative name(s):
Phosphoinositide phospholipase C-gamma plc-3Curated
Phospholipase C-gamma plc-3Curated
Short name:
PLC-gamma plc-3Curated
Gene namesi
Name:plc-3Imported
ORF Names:T01E8.3Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiT01E8.3; CE42905; WBGene00004038; plc-3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown causes sterility resulting from impaired spermatheca dilatation causing a defect in ovulation, and a severe decrease in sheath cell basal and peak ovulatory contractions (PubMed:15194811, PubMed:18369461). The few embryos laid are arrested at the embryonic stage (PubMed:18369461). Cycles of posterior body wall muscle contractions are prolonged and arrhythmic resulting in a defecation defect (PubMed:16186564). Ca2+ oscillations in intestinal cells are arrhythmic and the whole cell current amplitude is severely reduced (PubMed:16186564, PubMed:19923421). RNAi-mediated knockdown in males causes an increase in spontaneous muscle seizures in the absence of food in an unc-103 mutant background (PubMed:18604269). Ca2+ transient increase and avoidance behavior are defective in response to nose touch but not to benzaldehyde (PubMed:19730689). nlp-29 expression is abrogated following fungal infection by D.coniospora and severely reduced following physical injury (PubMed:19380113).7 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004374631 – 13501-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma plc-3CuratedAdd BLAST1350

Proteomic databases

EPDiQ22070.
PaxDbiQ22070.
PeptideAtlasiQ22070.

Expressioni

Tissue specificityi

Expressed in intestine, isthmus of the pharynx, proximal gonad sheath cells, spermatheca and uterine sheath cells (PubMed:15194811, PubMed:16186564). In males, expressed in the valve cell, the vas deferens and retractor and ventral protactor muscles (PubMed:15958491, PubMed:18604269).4 Publications

Interactioni

Protein-protein interaction databases

IntActiQ22070. 2 interactors.
STRINGi6239.T01E8.3.

Structurei

3D structure databases

ProteinModelPortaliQ22070.
SMRiQ22070.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini352 – 503PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST152
Domaini605 – 704SH2 1PROSITE-ProRule annotationAdd BLAST100
Domaini715 – 804SH2 2PROSITE-ProRule annotationAdd BLAST90
Domaini832 – 890SH3PROSITE-ProRule annotationAdd BLAST59
Domaini855 – 960PHPROSITE-ProRule annotationAdd BLAST106
Domaini982 – 1092PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST111
Domaini1098 – 1203C2PROSITE-ProRule annotationAdd BLAST106

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation
Contains 2 SH2 domains.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG1264. Eukaryota.
ENOG410XPXE. LUCA.
GeneTreeiENSGT00730000110782.
HOGENOMiHOG000230864.
InParanoidiQ22070.
KOiK01116.
OMAiPANREEK.
OrthoDBiEOG7W419X.
PhylomeDBiQ22070.

Family and domain databases

Gene3Di2.30.29.30. 3 hits.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
3.30.505.10. 2 hits.
InterProiIPR000008. C2_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001192. PI-PLC_fam.
IPR016279. PLC-gamma.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
IPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 2 hits.
PfamiPF00168. C2. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF00017. SH2. 2 hits.
PF00018. SH3_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000952. PLC-gamma. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
PR00401. SH2DOMAIN.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
SM00252. SH2. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 2 hits.
SSF51695. SSF51695. 2 hits.
SSF55550. SSF55550. 3 hits.
PROSITEiPS50004. C2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
PS50001. SH2. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q22070-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQHGSLGPSS SSRKTTVTST AGSVHLHHRS SNGFSTASRA QLNRHNEMDF
60 70 80 90 100
GKMFAAMEKG HKVCKIAVLK KWDPAYKQLT LDRYSRQIFL TKLELSAVRN
110 120 130 140 150
KPTILDIRQI REVQTLNYKL NTIKVDDKWK KDREIVNFDS KAILIISYGM
160 170 180 190 200
GFALSYWILL FESEDACKLW SQGLHNLMMD TRDRDSSPHP LRIERFLHKH
210 220 230 240 250
FLSLMSPAND AVARKHMKPF VQTSLQFKVQ SDQLQQVTED QMNSDQFSFA
260 270 280 290 300
SRQLIHVPEL FSSRFADLAE KHERKGLVVT FQNFLRFLDG KQFDELASNR
310 320 330 340 350
TRALEFLNRY FQEDQAYFGD RNEPSMTVFE FCDFLFSREN SLWDPTNEKV
360 370 380 390 400
THDMSRPLSH YWIASSHNTY LTGDQLRSES SLDCYAQALL MGCRCIELDC
410 420 430 440 450
WDGQKKPGSA EFIDIVIYHG YTMTSKILLR DVLHVIRHYA FVTSEYPVIL
460 470 480 490 500
SIEDNCSVPA QRLLAQELKD ILGDYLLTQP ANREEKQLPS PAALKKKIIV
510 520 530 540 550
KHKKLPIESE DLAAVVKTDE FQDTEIISRE CVKKGILMVK NNNSHEWTSH
560 570 580 590 600
VFILFPDRLC YLIQTADPEN PNDDTVSVSG DEEREEETPS GFGVKPEEMH
610 620 630 640 650
VTEEWFHGRC ERDEAKKRIL EHKEKGNGLF MIRDSNLFIG DFSLSILHDG
660 670 680 690 700
KVHHVRIRSK IIDKEKKYYF MDNKVCDTLY ELVSYYTRHY LTTAHFKMVL
710 720 730 740 750
TIPCPQPQPH LNQPWFSATA DKEKAEELLS LVPEDGAFLI RTSSTDSSVY
760 770 780 790 800
VLSLKVDGEF WHYRLKRDGR IFVVNQKVFE NLNQIVEFYA NREFVRGISL
810 820 830 840 850
RFPVNEKDIS HLTAELAEAR TPGCYMDLKD LDKEVQARAL RPYRGTADDE
860 870 880 890 900
LSFPANVIIT VLRKEEGLWR GRYGSLTGWF PSAHVQEILP EKVSTSETSN
910 920 930 940 950
YNTIELAGTL IERIHDADRP NVIRISQSNQ HWMNKQYYLL AASSSEEADG
960 970 980 990 1000
WQNNLFELTR SVNTKMSILR TKEKEKRIAA ELSNLVVYCQ AVPFDPAHIY
1010 1020 1030 1040 1050
NDAFYEMCSF VEGKLDKLVE KGLLPFNSRK LSRVYPNGSR ITSNNYSPVP
1060 1070 1080 1090 1100
MWNAGCHMVA LNYQTGDKPM QLNQGKFLAN GRCGYLLKPD YMLTDDFDPT
1110 1120 1130 1140 1150
NTEKFATAYP IRLNVQVIGG RHLSRKDKNK GICSPFVEIE IIGMPCDTKV
1160 1170 1180 1190 1200
FQTKTIASNG LNPIWNQTFT FEIQCPEVAL IRFHVEDGDF VGPKTDPFIG
1210 1220 1230 1240 1250
QAVFPVDSIR CGFRSVPLKN QYSEELELSS LLVDVQMCSR EGTQLIRSSS
1260 1270 1280 1290 1300
HFLQASRLAP VFAHRKITNG DSIPREMAPR LRTSATDRSL DSPTNSESRA
1310 1320 1330 1340 1350
TLLSGQRGSQ DSMDSAAETS SIASGTISSR DGKKKQNWLK KFSFGKSSKS
Length:1,350
Mass (Da):154,574
Last modified:September 23, 2008 - v2
Checksum:i8F636BE56E7F58F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284602 Genomic DNA. Translation: CAA88745.2.
RefSeqiNP_496205.2. NM_063804.4.
UniGeneiCel.14497.

Genome annotation databases

EnsemblMetazoaiT01E8.3; T01E8.3; WBGene00004038.
GeneIDi174586.
KEGGicel:CELE_T01E8.3.
UCSCiT01E8.3. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284602 Genomic DNA. Translation: CAA88745.2.
RefSeqiNP_496205.2. NM_063804.4.
UniGeneiCel.14497.

3D structure databases

ProteinModelPortaliQ22070.
SMRiQ22070.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ22070. 2 interactors.
STRINGi6239.T01E8.3.

Proteomic databases

EPDiQ22070.
PaxDbiQ22070.
PeptideAtlasiQ22070.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT01E8.3; T01E8.3; WBGene00004038.
GeneIDi174586.
KEGGicel:CELE_T01E8.3.
UCSCiT01E8.3. c. elegans.

Organism-specific databases

CTDi174586.
WormBaseiT01E8.3; CE42905; WBGene00004038; plc-3.

Phylogenomic databases

eggNOGiKOG1264. Eukaryota.
ENOG410XPXE. LUCA.
GeneTreeiENSGT00730000110782.
HOGENOMiHOG000230864.
InParanoidiQ22070.
KOiK01116.
OMAiPANREEK.
OrthoDBiEOG7W419X.
PhylomeDBiQ22070.

Enzyme and pathway databases

ReactomeiR-CEL-114604. GPVI-mediated activation cascade.
R-CEL-1169408. ISG15 antiviral mechanism.
R-CEL-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-CEL-202433. Generation of second messenger molecules.
R-CEL-5607764. CLEC7A (Dectin-1) signaling.
R-CEL-5654219. Phospholipase C-mediated cascade: FGFR1.
R-CEL-5654221. Phospholipase C-mediated cascade, FGFR2.
R-CEL-5654227. Phospholipase C-mediated cascade, FGFR3.
R-CEL-5654228. Phospholipase C-mediated cascade, FGFR4.
SignaLinkiQ22070.

Family and domain databases

Gene3Di2.30.29.30. 3 hits.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
3.30.505.10. 2 hits.
InterProiIPR000008. C2_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001192. PI-PLC_fam.
IPR016279. PLC-gamma.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
IPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 2 hits.
PfamiPF00168. C2. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF00017. SH2. 2 hits.
PF00018. SH3_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000952. PLC-gamma. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
PR00401. SH2DOMAIN.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
SM00252. SH2. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 2 hits.
SSF51695. SSF51695. 2 hits.
SSF55550. SSF55550. 3 hits.
PROSITEiPS50004. C2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
PS50001. SH2. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLCG_CAEEL
AccessioniPrimary (citable) accession number: Q22070
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2016
Last sequence update: September 23, 2008
Last modified: November 30, 2016
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.