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Protein

Ras-like GTP-binding protein rhoA

Gene

rho-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for ventral migration of epidermal cells during ventral enclosure in the embryo and for cell elongation. Also required for ventral migration of P cells during larval development. Involved in asymmetric spindle positioning during anaphase and establishment of cell polarity during embryo development. In adults, involved in regulation of multiple processes including locomotion, pharyngeal pumping, fecundity, ovulation, defecation and body morphology.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 19GTPBy similarity8
Nucleotide bindingi59 – 63GTPBy similarity5
Nucleotide bindingi117 – 120GTPBy similarity4

GO - Molecular functioni

  • GTPase activating protein binding Source: WormBase
  • GTPase activity Source: WormBase
  • GTP binding Source: WormBase
  • protein kinase binding Source: WormBase

GO - Biological processi

  • actomyosin contractile ring assembly Source: WormBase
  • mitotic nuclear division Source: UniProtKB-KW
  • multicellular organism development Source: UniProtKB-KW
  • myosin filament assembly Source: WormBase
  • positive regulation of acetylcholine secretion, neurotransmission Source: WormBase
  • positive regulation of locomotion Source: WormBase
  • regulation of actin filament-based process Source: WormBase
  • skeletal muscle myosin thick filament assembly Source: WormBase
  • small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-114604. GPVI-mediated activation cascade.
R-CEL-193634. Axonal growth inhibition (RHOA activation).
R-CEL-194840. Rho GTPase cycle.
R-CEL-198203. PI3K/AKT activation.
R-CEL-392451. G beta:gamma signalling through PI3Kgamma.
R-CEL-3928663. EPHA-mediated growth cone collapse.
R-CEL-4086400. PCP/CE pathway.
R-CEL-416482. G alpha (12/13) signalling events.
R-CEL-416572. Sema4D induced cell migration and growth-cone collapse.
R-CEL-4420097. VEGFA-VEGFR2 Pathway.
R-CEL-5625740. RHO GTPases activate PKNs.
R-CEL-5625900. RHO GTPases activate CIT.
R-CEL-5627117. RHO GTPases Activate ROCKs.
R-CEL-5663220. RHO GTPases Activate Formins.
R-CEL-5666185. RHO GTPases Activate Rhotekin and Rhophilins.
R-CEL-5689896. Ovarian tumor domain proteases.
R-CEL-6798695. Neutrophil degranulation.
R-CEL-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-like GTP-binding protein rhoA
Gene namesi
Name:rho-1
Synonyms:rhoa
ORF Names:Y51H4A.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiY51H4A.3a; CE25369; WBGene00004357; rho-1.

Subcellular locationi

GO - Cellular componenti

  • cell cortex Source: WormBase
  • cleavage furrow Source: WormBase
  • cytoskeleton Source: UniProtKB-SubCell
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown of the protein causes embryonic arrest in most cases with development of elongation-defective uncoordinated kinky larvae from embryos which escape arrest. Defective ventral migration of P cells leading to defective gonad development. Defective establishment of anterior-posterior cell polarity in one-cell embryos.3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi14G → V: Constitutively active. Normal P cell migration. No effect on viability or fertility. 2 Publications1
Mutagenesisi19T → N: Dominant negative. Defective ventral migration of P cells leading to defective gonad development. Severely uncoordinated. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001988841 – 189Ras-like GTP-binding protein rhoAAdd BLAST189
PropeptideiPRO_0000281237190 – 192Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei189Cysteine methyl esterBy similarity1
Lipidationi189S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

EPDiQ22038.
PaxDbiQ22038.
PeptideAtlasiQ22038.
PRIDEiQ22038.

Expressioni

Tissue specificityi

In larvae and adults, enriched at the tip of the head where the anterior sensory organ is located and in the pharyngeal nerve ring (at protein level). In embryos, enriched at the boundaries of dorsal cells undergoing intercalation, ventral enclosure and elongation.1 Publication

Developmental stagei

Highest expression during larval development with a peak at L3 and lower levels in the embryo and adult (at protein level).2 Publications

Gene expression databases

BgeeiWBGene00004357.

Interactioni

GO - Molecular functioni

  • GTPase activating protein binding Source: WormBase
  • protein kinase binding Source: WormBase

Protein-protein interaction databases

BioGridi43536. 2 interactors.
IntActiQ22038. 2 interactors.
MINTiMINT-1515134.
STRINGi6239.Y51H4A.3.

Structurei

3D structure databases

ProteinModelPortaliQ22038.
SMRiQ22038.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi34 – 42Effector regionSequence analysis9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiKOG0393. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000119020.
HOGENOMiHOG000233974.
InParanoidiQ22038.
KOiK04513.
OMAiKRRGACQ.
OrthoDBiEOG091G0QVS.
PhylomeDBiQ22038.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q22038-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAIRKKLVI VGDGACGKTC LLIVFSKDQF PDVYVPTVFE NYVADIEVDG
60 70 80 90 100
KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSIDSPD SLENIPEKWT
110 120 130 140 150
PEVRHFCPNV PIILVGNKRD LRSDPQTVRE LAKMKQEPVK PEQGRAIAEQ
160 170 180 190
IGAFAYLECS AKTKDGIREV FEKATQAALQ QKKKKKSKCM IL
Length:192
Mass (Da):21,635
Last modified:November 1, 1997 - v1
Checksum:i0B10A744D8D0CF81
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36965 mRNA. Translation: AAC37216.1.
AL132952 Genomic DNA. Translation: CAB63379.1.
PIRiA55492.
RefSeqiNP_502959.1. NM_070558.6.
UniGeneiCel.17449.

Genome annotation databases

EnsemblMetazoaiY51H4A.3a; Y51H4A.3a; WBGene00004357.
GeneIDi178458.
KEGGicel:CELE_Y51H4A.3.
UCSCiY51H4A.3. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36965 mRNA. Translation: AAC37216.1.
AL132952 Genomic DNA. Translation: CAB63379.1.
PIRiA55492.
RefSeqiNP_502959.1. NM_070558.6.
UniGeneiCel.17449.

3D structure databases

ProteinModelPortaliQ22038.
SMRiQ22038.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi43536. 2 interactors.
IntActiQ22038. 2 interactors.
MINTiMINT-1515134.
STRINGi6239.Y51H4A.3.

Proteomic databases

EPDiQ22038.
PaxDbiQ22038.
PeptideAtlasiQ22038.
PRIDEiQ22038.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiY51H4A.3a; Y51H4A.3a; WBGene00004357.
GeneIDi178458.
KEGGicel:CELE_Y51H4A.3.
UCSCiY51H4A.3. c. elegans.

Organism-specific databases

CTDi178458.
WormBaseiY51H4A.3a; CE25369; WBGene00004357; rho-1.

Phylogenomic databases

eggNOGiKOG0393. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000119020.
HOGENOMiHOG000233974.
InParanoidiQ22038.
KOiK04513.
OMAiKRRGACQ.
OrthoDBiEOG091G0QVS.
PhylomeDBiQ22038.

Enzyme and pathway databases

ReactomeiR-CEL-114604. GPVI-mediated activation cascade.
R-CEL-193634. Axonal growth inhibition (RHOA activation).
R-CEL-194840. Rho GTPase cycle.
R-CEL-198203. PI3K/AKT activation.
R-CEL-392451. G beta:gamma signalling through PI3Kgamma.
R-CEL-3928663. EPHA-mediated growth cone collapse.
R-CEL-4086400. PCP/CE pathway.
R-CEL-416482. G alpha (12/13) signalling events.
R-CEL-416572. Sema4D induced cell migration and growth-cone collapse.
R-CEL-4420097. VEGFA-VEGFR2 Pathway.
R-CEL-5625740. RHO GTPases activate PKNs.
R-CEL-5625900. RHO GTPases activate CIT.
R-CEL-5627117. RHO GTPases Activate ROCKs.
R-CEL-5663220. RHO GTPases Activate Formins.
R-CEL-5666185. RHO GTPases Activate Rhotekin and Rhophilins.
R-CEL-5689896. Ovarian tumor domain proteases.
R-CEL-6798695. Neutrophil degranulation.
R-CEL-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.

Miscellaneous databases

PROiQ22038.

Gene expression databases

BgeeiWBGene00004357.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRHO1_CAEEL
AccessioniPrimary (citable) accession number: Q22038
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.