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Protein

Ras-like GTP-binding protein rhoA

Gene

rho-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for ventral migration of epidermal cells during ventral enclosure in the embryo and for cell elongation (PubMed:24016757). Also required for ventral migration of P cells during larval development (PubMed:11687661). Involved in asymmetric spindle positioning during anaphase and establishment of cell polarity during embryo development (PubMed:16921365). In adults, involved in regulation of multiple processes including locomotion, pharyngeal pumping, fecundity, ovulation, defecation and body morphology (PubMed:21387015). In body wall muscles, regulates organization of myosin thick filaments downstream of unc-89 (PubMed:18801371). Association with the oxidase bli-3 promotes ROS production and this interaction may be modulated by memo-1, in order to control the oxidative stress response and longevity (PubMed:28085666).6 Publications

Enzyme regulationi

GTP hydrolysis is stimulated by unc-89.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 19GTPBy similarity8
Nucleotide bindingi59 – 63GTPBy similarity5
Nucleotide bindingi117 – 120GTPBy similarity4

GO - Molecular functioni

  • GTPase activating protein binding Source: WormBase
  • GTPase activity Source: WormBase
  • GTP binding Source: WormBase
  • protein kinase binding Source: WormBase

GO - Biological processi

  • actomyosin contractile ring assembly Source: WormBase
  • multicellular organism development Source: UniProtKB-KW
  • myosin filament assembly Source: WormBase
  • positive regulation of acetylcholine secretion, neurotransmission Source: WormBase
  • positive regulation of locomotion Source: WormBase
  • regulation of actin filament-based process Source: WormBase
  • skeletal muscle myosin thick filament assembly Source: WormBase
  • small GTPase mediated signal transduction Source: InterPro

Keywordsi

Molecular functionDevelopmental protein
Biological processCell cycle, Cell division, Mitosis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-114604 GPVI-mediated activation cascade
R-CEL-193634 Axonal growth inhibition (RHOA activation)
R-CEL-194840 Rho GTPase cycle
R-CEL-198203 PI3K/AKT activation
R-CEL-392451 G beta:gamma signalling through PI3Kgamma
R-CEL-3928662 EPHB-mediated forward signaling
R-CEL-3928663 EPHA-mediated growth cone collapse
R-CEL-4086400 PCP/CE pathway
R-CEL-416482 G alpha (12/13) signalling events
R-CEL-416572 Sema4D induced cell migration and growth-cone collapse
R-CEL-4420097 VEGFA-VEGFR2 Pathway
R-CEL-5625740 RHO GTPases activate PKNs
R-CEL-5627117 RHO GTPases Activate ROCKs
R-CEL-5663220 RHO GTPases Activate Formins
R-CEL-5666185 RHO GTPases Activate Rhotekin and Rhophilins
R-CEL-5689896 Ovarian tumor domain proteases
R-CEL-6785631 ERBB2 Regulates Cell Motility
R-CEL-6798695 Neutrophil degranulation
R-CEL-8849471 PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases
R-CEL-8985586 SLIT2:ROBO1 increases RHOA activity

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-like GTP-binding protein rhoA
Gene namesi
Name:rho-1Imported
Synonyms:rhoa1 Publication
ORF Names:Y51H4A.3Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiY51H4A.3a ; CE25369 ; WBGene00004357 ; rho-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown predominantly results in embryonic arrest with surviving embryos developing elongation-defective uncoordinated kinky larvae (PubMed:24016757, PubMed:11687661). Defective ventral migration of P cells leading to defective gonad development (PubMed:11687661). Defective establishment of anterior-posterior cell polarity in one-cell embryos (PubMed:16921365). RNAi-mediated knockdown results in disorganized myosin thick filaments in the body wall muscles of the few surviving adults, which is characterized by the formation of abnormal myosin heavy chain myo-3 aggregates, V-shaped crossing of A-bands and areas in which the myosin heavy chain is missing (PubMed:18801371). RNAi-mediated knockdown suppresses reactive oxygen species induction and longevity in the memo-1 mutant (PubMed:28085666). Double RNAi-mediated knockdown with memo-1 eliminates the resistance to oxidative stress in the memo-1 single mutant (PubMed:28085666).5 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi14G → V: Constitutively active. Normal P cell migration. No effect on viability or fertility. 2 Publications1
Mutagenesisi19T → N: Dominant negative. Defective ventral migration of P cells leading to defective gonad development. Severely uncoordinated. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001988841 – 189Ras-like GTP-binding protein rhoAAdd BLAST189
PropeptideiPRO_0000281237190 – 192Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei189Cysteine methyl esterBy similarity1
Lipidationi189S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

EPDiQ22038
PaxDbiQ22038
PeptideAtlasiQ22038
PRIDEiQ22038

Expressioni

Tissue specificityi

In larvae and adults, enriched at the tip of the head where the anterior sensory organ is located and in the pharyngeal nerve ring (at protein level). In embryos, enriched at the boundaries of dorsal cells undergoing intercalation, ventral enclosure and elongation.1 Publication

Developmental stagei

Highest expression during larval development with a peak at L3 and lower levels in the embryo and adult (at protein level).2 Publications

Gene expression databases

BgeeiWBGene00004357

Interactioni

Subunit structurei

May interact with unc-89 (via DN and PH domains) (PubMed:18801371). Interacts with bli-3 and memo-1 (PubMed:28085666).2 Publications

GO - Molecular functioni

  • GTPase activating protein binding Source: WormBase
  • protein kinase binding Source: WormBase

Protein-protein interaction databases

BioGridi43536, 2 interactors
IntActiQ22038, 2 interactors
MINTiQ22038
STRINGi6239.Y51H4A.3

Structurei

3D structure databases

ProteinModelPortaliQ22038
SMRiQ22038
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi34 – 42Effector regionSequence analysis9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiKOG0393 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000119020
HOGENOMiHOG000233974
InParanoidiQ22038
KOiK04513
OMAiQKIGARH
OrthoDBiEOG091G0QVS
PhylomeDBiQ22038

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR003578 Small_GTPase_Rho
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51420 RHO, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q22038-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAIRKKLVI VGDGACGKTC LLIVFSKDQF PDVYVPTVFE NYVADIEVDG
60 70 80 90 100
KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSIDSPD SLENIPEKWT
110 120 130 140 150
PEVRHFCPNV PIILVGNKRD LRSDPQTVRE LAKMKQEPVK PEQGRAIAEQ
160 170 180 190
IGAFAYLECS AKTKDGIREV FEKATQAALQ QKKKKKSKCM IL
Length:192
Mass (Da):21,635
Last modified:November 1, 1997 - v1
Checksum:i0B10A744D8D0CF81
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36965 mRNA Translation: AAC37216.1
AL132952 Genomic DNA Translation: CAB63379.1
PIRiA55492
RefSeqiNP_502959.1, NM_070558.6
UniGeneiCel.17449

Genome annotation databases

EnsemblMetazoaiY51H4A.3a; Y51H4A.3a; WBGene00004357
GeneIDi178458
KEGGicel:CELE_Y51H4A.3
UCSCiY51H4A.3 c. elegans

Similar proteinsi

Entry informationi

Entry nameiRHO1_CAEEL
AccessioniPrimary (citable) accession number: Q22038
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health