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Protein

Calpain-5

Gene

tra-3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the correct female sexual development of the soma and germline in hermaphrodite animals, while being fully dispensable in males. Has calcium-dependent proteolytic activity and is involved in the cleavage of tra-2, for which it acts as a potentiator. Capable of calcium-dependent autolysis.1 Publication

Catalytic activityi

Broad endopeptidase specificity.1 Publication

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei83 – 8311 Publication
Active sitei252 – 2521By similarity
Active sitei290 – 2901By similarity

GO - Molecular functioni

  • calcium-dependent cysteine-type endopeptidase activity Source: WormBase
  • cysteine-type endopeptidase activity Source: UniProtKB

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • female germ-line sex determination Source: UniProtKB
  • feminization of hermaphrodite soma Source: UniProtKB
  • hermaphrodite somatic sex determination Source: WormBase
  • membrane protein intracellular domain proteolysis Source: WormBase
  • protein autoprocessing Source: WormBase
  • proteolysis Source: UniProtKB
  • sex differentiation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Differentiation, Sexual differentiation

Keywords - Ligandi

Calcium

Protein family/group databases

MEROPSiC02.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-5 (EC:3.4.22.-)
Alternative name(s):
Sex-determining transformer protein 3
Gene namesi
Name:tra-3Imported
Synonyms:clp-5Imported
ORF Names:LLC1.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiLLC1.1; CE16260; WBGene00006606; tra-3.

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: InterPro
  • membrane Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi83 – 831C → S: Prevents female sexual development. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 648648Calpain-5PRO_0000207716Add
BLAST

Proteomic databases

EPDiQ22036.
PaxDbiQ22036.

PTM databases

iPTMnetiQ22036.

Interactioni

Protein-protein interaction databases

STRINGi6239.LLC1.1.

Structurei

3D structure databases

ProteinModelPortaliQ22036.
SMRiQ22036. Positions 5-504.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 353326Calpain catalyticPROSITE-ProRule annotationAdd
BLAST
Domaini528 – 62497C2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni354 – 509156Domain IIIAdd
BLAST

Domaini

The calpain catalytic domain is essential for in vivo function to promote female sexual development.
The C2 domain associated with the domain III is primarily responsible for calcium binding.

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 C2 domain.Sequence analysis
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232036.
InParanoidiQ22036.
KOiK08574.
OMAiSFIMGSK.
OrthoDBiEOG75J0MJ.
PhylomeDBiQ22036.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00239. C2. 1 hit.
SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q22036-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRSEKTRHF GNQNYEKLRK ICIKKKQPFV DTLFPPTNQS LFLEQRQSSD
60 70 80 90 100
IVWKRPGELH PDPHLFVEGA SPNDVTQGIL GNCWFVSACS ALTHNFKLLA
110 120 130 140 150
QVIPDADDQE WSTKHAYAGI FRFRFWRFGK WVEVVIDDLL PTRDGKLLFA
160 170 180 190 200
RSKTPNEFWS ALLEKAFAKL YGCYENLVGG HLSDALQDVS GGVAETLHVR
210 220 230 240 250
KFLKDDPNDT ELKLFNDLKT AFDKGALVVA AIAARTKEEI EESLDCGLVK
260 270 280 290 300
GHAYAVSAVC TIDVTNPNER SFTSFIMGSK RKQNLIRLQN PWGEKEWNGA
310 320 330 340 350
WSDDSPEWQN VSASQLSTMG VQPANSDSDD GDFWMPWESF VHYFTDISLC
360 370 380 390 400
QLFNTSVFSF SRSYDEQIVF SEWTTNGKKS GAPDDRAGGC HNFKATFCNN
410 420 430 440 450
PQYIFDIPSP NCSVMFALIQ NDPSEGLKKR EPFVTIGMHV MKVENNRQYR
460 470 480 490 500
VHTAMHPIAI SDYASGRSVY LHLQSLPRGR YLLIPTTFAP KEQTLFMLRV
510 520 530 540 550
YSDEHIHFSP LTKHAPKLGL LKCKSAQSVT RLTIHGVDFN SASTGTHNVY
560 570 580 590 600
AILKDSRKSF RTKTLSGVKS IQWDEQFLFH KSKNRQQYKI EVWEDRKMAR
610 620 630 640
DHLLAQSVII ALIDNENRDT TLQLTDPRGT VIGTVSVTVS AFDDPMYL
Length:648
Mass (Da):73,614
Last modified:November 1, 1996 - v1
Checksum:iF6F56265FDCB80EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12921, U12516, U12920 Genomic DNA. Translation: AAB60256.1.
Z82277 Genomic DNA. Translation: CAB05248.1.
PIRiS71885.
RefSeqiNP_502751.1. NM_070350.4.
UniGeneiCel.12258.

Genome annotation databases

EnsemblMetazoaiLLC1.1a; LLC1.1a; WBGene00006606.
GeneIDi178385.
KEGGicel:CELE_LLC1.1.
UCSCiLLC1.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12921, U12516, U12920 Genomic DNA. Translation: AAB60256.1.
Z82277 Genomic DNA. Translation: CAB05248.1.
PIRiS71885.
RefSeqiNP_502751.1. NM_070350.4.
UniGeneiCel.12258.

3D structure databases

ProteinModelPortaliQ22036.
SMRiQ22036. Positions 5-504.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.LLC1.1.

Protein family/group databases

MEROPSiC02.009.

PTM databases

iPTMnetiQ22036.

Proteomic databases

EPDiQ22036.
PaxDbiQ22036.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiLLC1.1a; LLC1.1a; WBGene00006606.
GeneIDi178385.
KEGGicel:CELE_LLC1.1.
UCSCiLLC1.1. c. elegans.

Organism-specific databases

CTDi178385.
WormBaseiLLC1.1; CE16260; WBGene00006606; tra-3.

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232036.
InParanoidiQ22036.
KOiK08574.
OMAiSFIMGSK.
OrthoDBiEOG75J0MJ.
PhylomeDBiQ22036.

Miscellaneous databases

NextBioi900910.
PROiQ22036.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00239. C2. 1 hit.
SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The tra-3 sex determination gene of Caenorhabditis elegans encodes a member of the calpain regulatory protease family."
    Barnes T.M., Hodgkin J.
    EMBO J. 15:4477-4484(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Bristol N2Imported.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2Imported.
  3. "Proteolysis in Caenorhabditis elegans sex determination: cleavage of TRA-2A by TRA-3."
    Sokol S.B., Kuwabara P.E.
    Genes Dev. 14:901-906(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF CYS-83.

Entry informationi

Entry nameiCAN5_CAEEL
AccessioniPrimary (citable) accession number: Q22036
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: November 1, 1996
Last modified: March 16, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.