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Protein

Calpain-5

Gene

tra-3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the correct female sexual development of the soma and germline in hermaphrodite animals, while being fully dispensable in males. Has calcium-dependent proteolytic activity and is involved in the cleavage of tra-2, for which it acts as a potentiator. Capable of calcium-dependent autolysis.1 Publication

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei831 Publication1
Active sitei252By similarity1
Active sitei290By similarity1

GO - Molecular functioni

  • calcium-dependent cysteine-type endopeptidase activity Source: WormBase
  • cysteine-type endopeptidase activity Source: WormBase

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • female germ-line sex determination Source: UniProtKB
  • feminization of hermaphrodite soma Source: UniProtKB
  • hermaphrodite somatic sex determination Source: WormBase
  • membrane protein intracellular domain proteolysis Source: WormBase
  • protein autoprocessing Source: WormBase
  • proteolysis Source: UniProtKB
  • sex differentiation Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processDifferentiation, Sexual differentiation
LigandCalcium

Protein family/group databases

MEROPSiC02.009

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-5 (EC:3.4.22.-)
Alternative name(s):
Sex-determining transformer protein 3
Gene namesi
Name:tra-3Imported
Synonyms:clp-5Imported
ORF Names:LLC1.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiLLC1.1 ; CE16260 ; WBGene00006606 ; tra-3

Subcellular locationi

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi83C → S: Prevents female sexual development. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002077161 – 648Calpain-5Add BLAST648

Proteomic databases

EPDiQ22036
PaxDbiQ22036
PeptideAtlasiQ22036
PRIDEiQ22036

PTM databases

iPTMnetiQ22036

Expressioni

Gene expression databases

BgeeiWBGene00006606

Interactioni

Protein-protein interaction databases

STRINGi6239.LLC1.1

Structurei

3D structure databases

ProteinModelPortaliQ22036
SMRiQ22036
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 353Calpain catalyticPROSITE-ProRule annotationAdd BLAST326
Domaini528 – 624C2Add BLAST97

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni354 – 509Domain IIIAdd BLAST156

Domaini

The calpain catalytic domain is essential for in vivo function to promote female sexual development.
The C2 domain associated with the domain III is primarily responsible for calcium binding.

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated

Phylogenomic databases

eggNOGiKOG0045 Eukaryota
ENOG410XP0B LUCA
GeneTreeiENSGT00760000118971
HOGENOMiHOG000232036
InParanoidiQ22036
KOiK08574
OMAiMMIRLQN
OrthoDBiEOG091G030A
PhylomeDBiQ22036

Family and domain databases

CDDicd04046 C2_Calpain, 1 hit
cd00214 Calpain_III, 1 hit
cd00044 CysPc, 1 hit
Gene3Di2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR033884 C2_Calpain
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR033883 C2_III
IPR022684 Calpain_cysteine_protease
IPR022682 Calpain_domain_III
IPR022683 Calpain_III
IPR036213 Calpain_III_sf
IPR000169 Pept_cys_AS
IPR001300 Peptidase_C2_calpain_cat
PfamiView protein in Pfam
PF01067 Calpain_III, 1 hit
PF00648 Peptidase_C2, 1 hit
PRINTSiPR00704 CALPAIN
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00720 calpain_III, 1 hit
SM00230 CysPc, 1 hit
SUPFAMiSSF49758 SSF49758, 1 hit
PROSITEiView protein in PROSITE
PS50203 CALPAIN_CAT, 1 hit
PS00139 THIOL_PROTEASE_CYS, 1 hit

Sequencei

Sequence statusi: Complete.

Q22036-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRSEKTRHF GNQNYEKLRK ICIKKKQPFV DTLFPPTNQS LFLEQRQSSD
60 70 80 90 100
IVWKRPGELH PDPHLFVEGA SPNDVTQGIL GNCWFVSACS ALTHNFKLLA
110 120 130 140 150
QVIPDADDQE WSTKHAYAGI FRFRFWRFGK WVEVVIDDLL PTRDGKLLFA
160 170 180 190 200
RSKTPNEFWS ALLEKAFAKL YGCYENLVGG HLSDALQDVS GGVAETLHVR
210 220 230 240 250
KFLKDDPNDT ELKLFNDLKT AFDKGALVVA AIAARTKEEI EESLDCGLVK
260 270 280 290 300
GHAYAVSAVC TIDVTNPNER SFTSFIMGSK RKQNLIRLQN PWGEKEWNGA
310 320 330 340 350
WSDDSPEWQN VSASQLSTMG VQPANSDSDD GDFWMPWESF VHYFTDISLC
360 370 380 390 400
QLFNTSVFSF SRSYDEQIVF SEWTTNGKKS GAPDDRAGGC HNFKATFCNN
410 420 430 440 450
PQYIFDIPSP NCSVMFALIQ NDPSEGLKKR EPFVTIGMHV MKVENNRQYR
460 470 480 490 500
VHTAMHPIAI SDYASGRSVY LHLQSLPRGR YLLIPTTFAP KEQTLFMLRV
510 520 530 540 550
YSDEHIHFSP LTKHAPKLGL LKCKSAQSVT RLTIHGVDFN SASTGTHNVY
560 570 580 590 600
AILKDSRKSF RTKTLSGVKS IQWDEQFLFH KSKNRQQYKI EVWEDRKMAR
610 620 630 640
DHLLAQSVII ALIDNENRDT TLQLTDPRGT VIGTVSVTVS AFDDPMYL
Length:648
Mass (Da):73,614
Last modified:November 1, 1996 - v1
Checksum:iF6F56265FDCB80EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12921, U12516, U12920 Genomic DNA Translation: AAB60256.1
Z82277 Genomic DNA Translation: CAB05248.1
PIRiS71885
RefSeqiNP_502751.1, NM_070350.4
UniGeneiCel.12258

Genome annotation databases

EnsemblMetazoaiLLC1.1a; LLC1.1a; WBGene00006606
GeneIDi178385
KEGGicel:CELE_LLC1.1
UCSCiLLC1.1 c. elegans

Similar proteinsi

Entry informationi

Entry nameiCAN5_CAEEL
AccessioniPrimary (citable) accession number: Q22036
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health