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Protein

Probable FAD synthase

Gene

R53.1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme.By similarity

Catalytic activityi

ATP + FMN = diphosphate + FAD.

Cofactori

Mg2+By similarity

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. FMN adenylyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. FAD biosynthetic process Source: UniProtKB-UniPathway
  2. Mo-molybdopterin cofactor biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, FAD, Flavoprotein, FMN, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_238979. Vitamin B2 (riboflavin) metabolism.
UniPathwayiUPA00277; UER00407.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable FAD synthase (EC:2.7.7.2)
Alternative name(s):
FAD pyrophosphorylase
FMN adenylyltransferase
Flavin adenine dinucleotide synthase
Including the following 2 domains:
Molybdenum cofactor biosynthesis protein-like region
FAD synthase region
Gene namesi
ORF Names:R53.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome II

Organism-specific databases

WormBaseiR53.1a; CE02303; WBGene00011271.
R53.1b; CE38215; WBGene00011271.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 519519Probable FAD synthasePRO_0000302743Add
BLAST

Proteomic databases

PaxDbiQ22017.
PRIDEiQ22017.

Expressioni

Gene expression databases

ExpressionAtlasiQ22017. baseline.

Interactioni

Protein-protein interaction databases

STRINGi6239.R53.1a.1.

Structurei

3D structure databases

ProteinModelPortaliQ22017.
SMRiQ22017. Positions 14-195, 308-490.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni17 – 10892Molybdenum cofactor biosynthesis protein-likeAdd
BLAST
Regioni328 – 485158FAD synthaseAdd
BLAST

Domaini

The molybdenum cofactor biosynthesis protein-like region may not be functional.

Sequence similaritiesi

In the N-terminal section; belongs to the MoaB/Mog family.Curated
In the C-terminal section; belongs to the PAPS reductase family. FAD1 subfamily.Curated

Phylogenomic databases

eggNOGiCOG0175.
GeneTreeiENSGT00390000007266.
HOGENOMiHOG000007185.
InParanoidiQ22017.
KOiK00953.
OMAiSFMRINP.
PhylomeDBiQ22017.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.40.980.10. 1 hit.
InterProiIPR012183. FAD_synth_Mopterin-bd.
IPR001453. Mopterin-bd_dom.
IPR002500. PAPS_reduct.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00994. MoCF_biosynth. 1 hit.
PF01507. PAPS_reduct. 1 hit.
[Graphical view]
PIRSFiPIRSF036620. MPTbdFAD. 1 hit.
SMARTiSM00852. MoCF_biosynth. 1 hit.
[Graphical view]
SUPFAMiSSF53218. SSF53218. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform a (identifier: Q22017-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRAMFRTPRM PQRKTAAILV IGDEILKGTT RDTNSHFLCK RLHKLGVNIR
60 70 80 90 100
KISVIGDDIS EISREVQSAS GAYDYVITSG GVGPTHDDKT YLGLAHAFTD
110 120 130 140 150
QMQFSDEIRQ AVNRFLPTYT AKKRAEGVGE GLEEAVRLAT EKLCTIPKMS
160 170 180 190 200
QLLWGTQKIN GSLSTFPVVR ISNVVALPGV PKFCERAFDE LQDQLFPIEE
210 220 230 240 250
RQSLCFETLY TDLDEFDFSK KLTDLAAQFE DRNVQIGSYP ELKNKFFKTK
260 270 280 290 300
LTIETESSET MEAVVTSLRE LLAGHIVYYD SHAWLDIVTK WKAFKKRKAS
310 320 330 340 350
ENQIEFIQKL NEAESIVEEI VEKYPLEQIA LSFNGGKDCT VLLHLLRLKV
360 370 380 390 400
DEKYGPSTPI QGFHIMVEDQ FPEATQFIID AAKFYNIQVL EFPGPLKTGL
410 420 430 440 450
AALKKTRPSI IPVLMGSRAT DPNGKYMKTP VEWTDSDWPQ VLRVCPILNW
460 470 480 490 500
TYTDVWHMLR GLCVPYCKLY DQGYTSLGGR DNTVKHPALR IVSSDGREHY
510
LPAYKLHNDA EERCNRSNI
Length:519
Mass (Da):58,965
Last modified:November 1, 1996 - v1
Checksum:i8B53D20915C85B59
GO
Isoform b (identifier: Q22017-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.

Show »
Length:510
Mass (Da):57,817
Checksum:i907047BF3727BBAC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 99Missing in isoform b. CuratedVSP_027956

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z66515 Genomic DNA. Translation: CAA91346.1.
Z66515 Genomic DNA. Translation: CAI70410.1.
PIRiT24243.
RefSeqiNP_001022286.1. NM_001027115.2. [Q22017-1]
NP_001022287.1. NM_001027116.3. [Q22017-2]
UniGeneiCel.30862.

Genome annotation databases

EnsemblMetazoaiR53.1a.1; R53.1a.1; WBGene00011271. [Q22017-1]
R53.1a.2; R53.1a.2; WBGene00011271. [Q22017-1]
GeneIDi3565030.
KEGGicel:CELE_R53.1.
UCSCiR53.1a.1. c. elegans. [Q22017-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z66515 Genomic DNA. Translation: CAA91346.1.
Z66515 Genomic DNA. Translation: CAI70410.1.
PIRiT24243.
RefSeqiNP_001022286.1. NM_001027115.2. [Q22017-1]
NP_001022287.1. NM_001027116.3. [Q22017-2]
UniGeneiCel.30862.

3D structure databases

ProteinModelPortaliQ22017.
SMRiQ22017. Positions 14-195, 308-490.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.R53.1a.1.

Proteomic databases

PaxDbiQ22017.
PRIDEiQ22017.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiR53.1a.1; R53.1a.1; WBGene00011271. [Q22017-1]
R53.1a.2; R53.1a.2; WBGene00011271. [Q22017-1]
GeneIDi3565030.
KEGGicel:CELE_R53.1.
UCSCiR53.1a.1. c. elegans. [Q22017-1]

Organism-specific databases

CTDi3565030.
WormBaseiR53.1a; CE02303; WBGene00011271.
R53.1b; CE38215; WBGene00011271.

Phylogenomic databases

eggNOGiCOG0175.
GeneTreeiENSGT00390000007266.
HOGENOMiHOG000007185.
InParanoidiQ22017.
KOiK00953.
OMAiSFMRINP.
PhylomeDBiQ22017.

Enzyme and pathway databases

UniPathwayiUPA00277; UER00407.
ReactomeiREACT_238979. Vitamin B2 (riboflavin) metabolism.

Miscellaneous databases

NextBioi954909.
PROiQ22017.

Gene expression databases

ExpressionAtlasiQ22017. baseline.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.40.980.10. 1 hit.
InterProiIPR012183. FAD_synth_Mopterin-bd.
IPR001453. Mopterin-bd_dom.
IPR002500. PAPS_reduct.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00994. MoCF_biosynth. 1 hit.
PF01507. PAPS_reduct. 1 hit.
[Graphical view]
PIRSFiPIRSF036620. MPTbdFAD. 1 hit.
SMARTiSM00852. MoCF_biosynth. 1 hit.
[Graphical view]
SUPFAMiSSF53218. SSF53218. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.

Entry informationi

Entry nameiFLAD1_CAEEL
AccessioniPrimary (citable) accession number: Q22017
Secondary accession number(s): Q58AA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.